UniProtKB - P76081 (PAAE_ECOLI)
Protein
1,2-phenylacetyl-CoA epoxidase, subunit E
Gene
paaE
Organism
Escherichia coli (strain K12)
Status
Functioni
Component of 1,2-phenylacetyl-CoA epoxidase multicomponent enzyme system which catalyzes the reduction of phenylacetyl-CoA (PA-CoA) to form 1,2-epoxyphenylacetyl-CoA. The subunit E is a reductase with a preference for NADPH and FAD, capable of reducing cytochrome c.4 Publications
Cofactori
Protein has several cofactor binding sites:- [2Fe-2S] cluster1 PublicationNote: Binds 1 [2Fe-2S] cluster.1 Publication
- FAD1 Publication
: phenylacetate degradation Pathwayi
This protein is involved in the pathway phenylacetate degradation, which is part of Aromatic compound metabolism.View all proteins of this organism that are known to be involved in the pathway phenylacetate degradation and in Aromatic compound metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 299 | Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 304 | Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 307 | Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 337 | Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: EcoCyc
- electron transfer activity Source: InterPro
- flavin adenine dinucleotide binding Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- phenylacetate catabolic process Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Electron transport, Transport |
Ligand | 2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, NAD |
Enzyme and pathway databases
BioCyci | EcoCyc:G6713-MONOMER MetaCyc:G6713-MONOMER |
UniPathwayi | UPA00930 |
Names & Taxonomyi
Protein namesi | Recommended name: 1,2-phenylacetyl-CoA epoxidase, subunit E (EC:1.-.-.-)Alternative name(s): 1,2-phenylacetyl-CoA epoxidase, reductase subunit 1,2-phenylacetyl-CoA monooxygenase, subunit E |
Gene namesi | Name:paaE Synonyms:ydbR Ordered Locus Names:b1392, JW1387 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000058163 | 1 – 356 | 1,2-phenylacetyl-CoA epoxidase, subunit EAdd BLAST | 356 |
Proteomic databases
PaxDbi | P76081 |
PRIDEi | P76081 |
Expressioni
Inductioni
Activated by cAMP receptor protein (CRP), integration host factor (IHF) and by phenylacetyl-coenzyme A (PA-CoA) that prevents PaaX from binding its target sequences. Inhibited by PaaX.2 Publications
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260178, 205 interactors |
ComplexPortali | CPX-2844, paaABCE phenylacetyl-CoA monooxygenase complex |
IntActi | P76081, 2 interactors |
STRINGi | 511145.b1392 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 106 | FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST | 105 | |
Domaini | 262 – 354 | 2Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST | 93 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 112 – 228 | OxidoreductaseSequence analysisAdd BLAST | 117 |
Sequence similaritiesi
In the N-terminal section; belongs to the FAD-binding oxidoreductase type 6 family.Curated
Phylogenomic databases
eggNOGi | COG1018, Bacteria |
HOGENOMi | CLU_003827_14_1_6 |
InParanoidi | P76081 |
PhylomeDBi | P76081 |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
Gene3Di | 3.10.20.30, 1 hit 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR006058, 2Fe2S_fd_BS IPR012675, Beta-grasp_dom_sf IPR008333, Cbr1-like_FAD-bd_dom IPR017927, FAD-bd_FR_type IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR039261, FNR_nucleotide-bd IPR001433, OxRdtase_FAD/NAD-bd IPR011884, PA_CoA_Oase5 IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00970, FAD_binding_6, 1 hit PF00111, Fer2, 1 hit PF00175, NAD_binding_1, 1 hit |
PRINTSi | PR00371, FPNCR |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF54292, SSF54292, 1 hit SSF63380, SSF63380, 1 hit |
TIGRFAMsi | TIGR02160, PA_CoA_Oxy5, 1 hit |
PROSITEi | View protein in PROSITE PS00197, 2FE2S_FER_1, 1 hit PS51085, 2FE2S_FER_2, 1 hit PS51384, FAD_FR, 1 hit |
i Sequence
Sequence statusi: Complete.
P76081-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTTFHSLTVA KVESETRDAV TITFAVPQPL QEAYRFRPGQ HLTLKASFDG
60 70 80 90 100
EELRRCYSIC RSYLPGEISV AVKAIEGGRF SRYAREHIRQ GMTLEVMVPQ
110 120 130 140 150
GHFGYQPQAE RQGRYLAIAA GSGITPMLAI IATTLQTEPE SQFTLIYGNR
160 170 180 190 200
TSQSMMFRQA LADLKDKYPQ RLQLLCIFSQ ETLDSDLLHG RIDGEKLQSL
210 220 230 240 250
GASLINFRLY DEAFICGPAA MMDDAETALK ALGMPDKTIH LERFNTPGTR
260 270 280 290 300
VKRSVNVQSD GQKVTVRQDG RDREIVLNAD DESILDAALR QGADLPYACK
310 320 330 340 350
GGVCATCKCK VLRGKVAMET NYSLEPDELA AGYVLSCQAL PLTSDVVVDF
DAKGMA
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 14 | S → P in strain: W. | 1 | |
Natural varianti | 169 | P → S in strain: W. | 1 | |
Natural varianti | 224 – 227 | DAET → ETEA in strain: W. | 4 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X97452 Genomic DNA Translation: CAA66094.1 U00096 Genomic DNA Translation: AAC74474.1 AP009048 Genomic DNA Translation: BAA14998.2 |
PIRi | C64890 |
RefSeqi | NP_415910.1, NC_000913.3 WP_000206388.1, NZ_SSZK01000012.1 |
Genome annotation databases
EnsemblBacteriai | AAC74474; AAC74474; b1392 BAA14998; BAA14998; BAA14998 |
GeneIDi | 57732132 945962 |
KEGGi | ecj:JW1387 eco:b1392 |
PATRICi | fig|1411691.4.peg.879 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X97452 Genomic DNA Translation: CAA66094.1 U00096 Genomic DNA Translation: AAC74474.1 AP009048 Genomic DNA Translation: BAA14998.2 |
PIRi | C64890 |
RefSeqi | NP_415910.1, NC_000913.3 WP_000206388.1, NZ_SSZK01000012.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4260178, 205 interactors |
ComplexPortali | CPX-2844, paaABCE phenylacetyl-CoA monooxygenase complex |
IntActi | P76081, 2 interactors |
STRINGi | 511145.b1392 |
Proteomic databases
PaxDbi | P76081 |
PRIDEi | P76081 |
Genome annotation databases
EnsemblBacteriai | AAC74474; AAC74474; b1392 BAA14998; BAA14998; BAA14998 |
GeneIDi | 57732132 945962 |
KEGGi | ecj:JW1387 eco:b1392 |
PATRICi | fig|1411691.4.peg.879 |
Organism-specific databases
EchoBASEi | EB3502 |
Phylogenomic databases
eggNOGi | COG1018, Bacteria |
HOGENOMi | CLU_003827_14_1_6 |
InParanoidi | P76081 |
PhylomeDBi | P76081 |
Enzyme and pathway databases
UniPathwayi | UPA00930 |
BioCyci | EcoCyc:G6713-MONOMER MetaCyc:G6713-MONOMER |
Miscellaneous databases
PROi | PR:P76081 |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
Gene3Di | 3.10.20.30, 1 hit 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR006058, 2Fe2S_fd_BS IPR012675, Beta-grasp_dom_sf IPR008333, Cbr1-like_FAD-bd_dom IPR017927, FAD-bd_FR_type IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR039261, FNR_nucleotide-bd IPR001433, OxRdtase_FAD/NAD-bd IPR011884, PA_CoA_Oase5 IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00970, FAD_binding_6, 1 hit PF00111, Fer2, 1 hit PF00175, NAD_binding_1, 1 hit |
PRINTSi | PR00371, FPNCR |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF54292, SSF54292, 1 hit SSF63380, SSF63380, 1 hit |
TIGRFAMsi | TIGR02160, PA_CoA_Oxy5, 1 hit |
PROSITEi | View protein in PROSITE PS00197, 2FE2S_FER_1, 1 hit PS51085, 2FE2S_FER_2, 1 hit PS51384, FAD_FR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PAAE_ECOLI | |
Accessioni | P76081Primary (citable) accession number: P76081 Secondary accession number(s): O53013, P77233 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | February 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 162 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families