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Entry version 147 (23 Feb 2022)
Sequence version 1 (01 Feb 1997)
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Protein

D-guloside 3-dehydrogenase

Gene

ycjQ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the NAD+-dependent oxidation of the hydroxyl group at C3 of D-gulosides leading to 3-dehydro-D-gulosides. Probably functions in a metabolic pathway that transforms D-gulosides to D-glucosides. Is also able to catalyze the reverse reactions, i.e. the NADH-dependent reduction of the oxo group at C3 of 3-dehydro-D-gulosides leading to D-gulosides. In vitro, can oxidize D-gulose and methyl beta-D-guloside, and reduce methyl alpha-3-dehydro-D-guloside and methyl beta-3-dehydro-D-guloside. However, the actual specific physiological substrates for this metabolic pathway are unknown.

1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Zn2+1 PublicationNote: Binds 1 Zn2+ ions per subunit.1 Publication

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 0.18 sec(-1) for the NAD+-dependent oxidation of D-gulose (at pH 8.0 and 30 degrees Celsius). kcat is 0.23 sec(-1) for the NAD+-dependent oxidation of D-gulose (at pH 9.0 and 30 degrees Celsius). kcat is 0.35 sec(-1) for the NAD+-dependent oxidation of methyl beta-D-guloside (at pH 8.0 and 30 degrees Celsius). kcat is 1.2 sec(-1) for the NAD+-dependent oxidation of methyl beta-D-guloside (at pH 9.0 and 30 degrees Celsius). kcat is 18.5 sec(-1) for the NADH-dependent reduction of methyl alpha-3-dehydro-D-guloside (at pH 7.0 and 30 degrees Celsius). kcat is 3.8 sec(-1) for the NADH-dependent reduction of methyl alpha-3-dehydro-D-guloside (at pH 8.0 and 30 degrees Celsius). kcat is 7.0 sec(-1) for the NADH-dependent reduction of methyl beta-3-dehydro-D-guloside (at pH 7.0 and 30 degrees Celsius). kcat is 9.7 sec(-1) for the NADH-dependent reduction of methyl beta-3-dehydro-D-guloside (at pH 8.0 and 30 degrees Celsius).1 Publication
  1. KM=5.1 mM for D-gulose (at pH 8.0 and 30 degrees Celsius)1 Publication
  2. KM=5.6 mM for D-gulose (at pH 9.0 and 30 degrees Celsius)1 Publication
  3. KM=12.5 mM for methyl beta-D-guloside (at pH 8.0 and 30 degrees Celsius)1 Publication
  4. KM=12.0 mM for methyl beta-D-guloside (at pH 9.0 and 30 degrees Celsius)1 Publication
  5. KM=9.4 mM for methyl alpha-3-dehydro-D-guloside (at pH 7.0 and 30 degrees Celsius)1 Publication
  6. KM=1.5 mM for methyl alpha-3-dehydro-D-guloside (at pH 8.0 and 30 degrees Celsius)1 Publication
  7. KM=2.3 mM for methyl beta-3-dehydro-D-guloside (at pH 7.0 and 30 degrees Celsius)1 Publication
  8. KM=7.0 mM for methyl beta-3-dehydro-D-guloside (at pH 8.0 and 30 degrees Celsius)1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionOxidoreductase
Biological processCarbohydrate metabolism
LigandMetal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:G6651-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
D-guloside 3-dehydrogenase1 Publication (EC:1.1.1.-1 Publication)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:ycjQ
Ordered Locus Names:b1313, JW1306
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001608901 – 350D-guloside 3-dehydrogenaseAdd BLAST350

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P76043

PRoteomics IDEntifications database

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PRIDEi
P76043

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
4263191, 8 interactors

Database of interacting proteins

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DIPi
DIP-11608N

STRING: functional protein association networks

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STRINGi
511145.b1313

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
P76043

Database of comparative protein structure models

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ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

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eggNOGi
COG1063, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_026673_9_0_6

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
P76043

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P76043

Family and domain databases

Integrated resource of protein families, domains and functional sites

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InterProi
View protein in InterPro
IPR013149, ADH_C
IPR011032, GroES-like_sf
IPR036291, NAD(P)-bd_dom_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00107, ADH_zinc_N, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF50129, SSF50129, 1 hit
SSF51735, SSF51735, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P76043-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKKLVATAPR VAALVEYEDR AILANEVKIR VRFGAPKHGT EVVDFRAASP
60 70 80 90 100
FIDEDFNGEW QMFTPRPADA PRGIEFGKFQ LGNMVVGDII ECGSDVTDYA
110 120 130 140 150
VGDSVCGYGP LSETVIINAV NNYKLRKMPQ GSSWKNAVCY DPAQFAMSGV
160 170 180 190 200
RDANVRVGDF VVVVGLGAIG QIAIQLAKRA GASVVIGVDP IAHRCDIARR
210 220 230 240 250
HGADFCLNPI GTDVGKEIKT LTGKQGADVI IETSGYADAL QSALRGLAYG
260 270 280 290 300
GTISYVAFAK PFAEGFNLGR EAHFNNAKIV FSRACSEPNP DYPRWSRKRI
310 320 330 340 350
EETCWELLMN GYLNCEDLID PVVTFANSPE SYMQYVDQHP EQSIKMGVTF
Length:350
Mass (Da):38,217
Last modified:February 1, 1997 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iA539943AF7A9ECB7
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

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DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74395.1
AP009048 Genomic DNA Translation: BAA14889.2

Protein sequence database of the Protein Information Resource

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PIRi
D64880

NCBI Reference Sequences

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RefSeqi
NP_415829.1, NC_000913.3
WP_000737347.1, NZ_SSZK01000012.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC74395; AAC74395; b1313
BAA14889; BAA14889; BAA14889

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
66674859
945971

KEGG: Kyoto Encyclopedia of Genes and Genomes

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KEGGi
ecj:JW1306
eco:b1313

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.966

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74395.1
AP009048 Genomic DNA Translation: BAA14889.2
PIRiD64880
RefSeqiNP_415829.1, NC_000913.3
WP_000737347.1, NZ_SSZK01000012.1

3D structure databases

SMRiP76043
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi4263191, 8 interactors
DIPiDIP-11608N
STRINGi511145.b1313

Proteomic databases

PaxDbiP76043
PRIDEiP76043

Genome annotation databases

EnsemblBacteriaiAAC74395; AAC74395; b1313
BAA14889; BAA14889; BAA14889
GeneIDi66674859
945971
KEGGiecj:JW1306
eco:b1313
PATRICifig|1411691.4.peg.966

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB3673

Phylogenomic databases

eggNOGiCOG1063, Bacteria
HOGENOMiCLU_026673_9_0_6
InParanoidiP76043
PhylomeDBiP76043

Enzyme and pathway databases

BioCyciEcoCyc:G6651-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P76043

Family and domain databases

InterProiView protein in InterPro
IPR013149, ADH_C
IPR011032, GroES-like_sf
IPR036291, NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00107, ADH_zinc_N, 1 hit
SUPFAMiSSF50129, SSF50129, 1 hit
SSF51735, SSF51735, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiYCJQ_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P76043
Secondary accession number(s): P78306
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: February 23, 2022
This is version 147 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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