UniProtKB - P76037 (PUUP_ECOLI)
Protein
Putrescine importer PuuP
Gene
puuP
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in the uptake of putrescine (PubMed:15590624, PubMed:19181795, PubMed:27803167, PubMed:23719730). Imports putrescine for its utilization as an energy source in the absence of glucose (PubMed:23719730).4 Publications
Activity regulationi
Inhibited by CCCP.1 Publication
Kineticsi
- KM=3.7 µM for putrescine1 Publication
- KM=6.2 µM for putrscine1 Publication
- Vmax=19.9 nmol/min/mg enzyme1 Publication
- Vmax=8.3 nmol/min/mg enzyme1 Publication
: putrescine degradation Pathwayi
This protein is involved in the pathway putrescine degradation, which is part of Amine and polyamine degradation.View all proteins of this organism that are known to be involved in the pathway putrescine degradation and in Amine and polyamine degradation.
GO - Molecular functioni
- putrescine transmembrane transporter activity Source: EcoCyc
- solute:proton symporter activity Source: EcoCyc
GO - Biological processi
- amino acid transport Source: UniProtKB-KW
- cellular response to DNA damage stimulus Source: EcoliWiki
- putrescine catabolic process Source: EcoCyc
- putrescine transport Source: EcoCyc
Keywordsi
Biological process | Amino-acid transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:B1296-MONOMER MetaCyc:B1296-MONOMER |
UniPathwayi | UPA00188 |
Protein family/group databases
TCDBi | 2.A.3.1.13, the amino acid-polyamine-organocation (apc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Putrescine importer PuuP |
Gene namesi | Name:puuP Synonyms:ycjJ Ordered Locus Names:b1296, JW1289 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane Curated; Multi-pass membrane protein Curated
Plasma Membrane
- plasma membrane Source: EcoCyc
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 27 – 47 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 54 – 74 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 100 – 120 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 125 – 145 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 162 – 182 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 204 – 224 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 242 – 262 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 275 – 295 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 344 – 364 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 365 – 385 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 399 – 419 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 422 – 442 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
No visible growth phenotype, no change in extracellular putrescine levels, if the putrescine export operon sapBCDF is also deleted growth is slower.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 110 | Y → X: The uptake activity is reduced to one-eighth of that of wild-type. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000054211 | 1 – 461 | Putrescine importer PuuPAdd BLAST | 461 |
Proteomic databases
PaxDbi | P76037 |
PRIDEi | P76037 |
Expressioni
Inductioni
Repressed by PuuR.2 Publications
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260139, 3 interactors |
DIPi | DIP-11601N |
IntActi | P76037, 3 interactors |
STRINGi | 511145.b1296 |
Family & Domainsi
Sequence similaritiesi
Belongs to the amino acid-polyamine-organocation (APC) superfamily.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0531, Bacteria |
HOGENOMi | CLU_007946_6_0_6 |
InParanoidi | P76037 |
PhylomeDBi | P76037 |
Family and domain databases
InterProi | View protein in InterPro IPR004841, AA-permease/SLC12A_dom |
Pfami | View protein in Pfam PF00324, AA_permease, 1 hit |
i Sequence
Sequence statusi: Complete.
P76037-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAINSPLNIA AQPGKTRLRK SLKLWQVVMM GLAYLTPMTV FDTFGIVSGI
60 70 80 90 100
SDGHVPASYL LALAGVLFTA ISYGKLVRQF PEAGSAYTYA QKSINPHVGF
110 120 130 140 150
MVGWSSLLDY LFLPMINVLL AKIYLSALFP EVPPWVWVVT FVAILTAANL
160 170 180 190 200
KSVNLVANFN TLFVLVQISI MVVFIFLVVQ GLHKGEGVGT VWSLQPFISE
210 220 230 240 250
NAHLIPIITG ATIVCFSFLG FDAVTTLSEE TPDAARVIPK AIFLTAVYGG
260 270 280 290 300
VIFIAASFFM QLFFPDISRF KDPDAALPEI ALYVGGKLFQ SIFLCTTFVN
310 320 330 340 350
TLASGLASHA SVSRLLYVMG RDNVFPERVF GYVHPKWRTP ALNVIMVGIV
360 370 380 390 400
ALSALFFDLV TATALINFGA LVAFTFVNLS VFNHFWRRKG MNKSWKDHFH
410 420 430 440 450
YLLMPLVGAL TVGVLWVNLE STSLTLGLVW ASLGGAYLWY LIRRYRKVPL
460
YDGDRTPVSE T
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74378.2 AP009048 Genomic DNA Translation: BAA14856.2 |
PIRi | C64878 |
RefSeqi | NP_415812.4, NC_000913.3 WP_000996856.1, NZ_SSZK01000012.1 |
Genome annotation databases
EnsemblBacteriai | AAC74378; AAC74378; b1296 BAA14856; BAA14856; BAA14856 |
GeneIDi | 57732066 946287 |
KEGGi | ecj:JW1289 eco:b1296 |
PATRICi | fig|511145.12.peg.1351 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74378.2 AP009048 Genomic DNA Translation: BAA14856.2 |
PIRi | C64878 |
RefSeqi | NP_415812.4, NC_000913.3 WP_000996856.1, NZ_SSZK01000012.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4260139, 3 interactors |
DIPi | DIP-11601N |
IntActi | P76037, 3 interactors |
STRINGi | 511145.b1296 |
Protein family/group databases
TCDBi | 2.A.3.1.13, the amino acid-polyamine-organocation (apc) family |
Proteomic databases
PaxDbi | P76037 |
PRIDEi | P76037 |
Genome annotation databases
EnsemblBacteriai | AAC74378; AAC74378; b1296 BAA14856; BAA14856; BAA14856 |
GeneIDi | 57732066 946287 |
KEGGi | ecj:JW1289 eco:b1296 |
PATRICi | fig|511145.12.peg.1351 |
Organism-specific databases
EchoBASEi | EB3666 |
Phylogenomic databases
eggNOGi | COG0531, Bacteria |
HOGENOMi | CLU_007946_6_0_6 |
InParanoidi | P76037 |
PhylomeDBi | P76037 |
Enzyme and pathway databases
UniPathwayi | UPA00188 |
BioCyci | EcoCyc:B1296-MONOMER MetaCyc:B1296-MONOMER |
Miscellaneous databases
PROi | PR:P76037 |
Family and domain databases
InterProi | View protein in InterPro IPR004841, AA-permease/SLC12A_dom |
Pfami | View protein in Pfam PF00324, AA_permease, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PUUP_ECOLI | |
Accessioni | P76037Primary (citable) accession number: P76037 Secondary accession number(s): P77557 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | December 15, 1998 | |
Last modified: | February 10, 2021 | |
This is version 157 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families