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Entry version 155 (17 Jun 2020)
Sequence version 2 (01 Dec 2000)
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Protein

PTS-dependent dihydroxyacetone kinase operon regulatory protein

Gene

dhaR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Positively regulates the dhaKLM operon from a sigma-70 promoter. Represses its own expression.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi355 – 362ATPPROSITE-ProRule annotation8
Nucleotide bindingi415 – 424ATPPROSITE-ProRule annotation10

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processGlycerol metabolism, Transcription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:G6628-MONOMER
ECOL316407:JW5188-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
PTS-dependent dihydroxyacetone kinase operon regulatory protein1 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:dhaR1 Publication
Synonyms:ycgU
Ordered Locus Names:b1201, JW5188
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000813201 – 639PTS-dependent dihydroxyacetone kinase operon regulatory proteinAdd BLAST639

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P76016

PRoteomics IDEntifications database

More...
PRIDEi
P76016

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Represses its own expression. DhaL and DhaK act antagonistically as coactivator and corepressor of the transcription activator by mutually exclusive binding to the sensing domain of DhaR. In the presence of dihydroxyacetone, DhaK that binds dihydroxyacetone has less affinity for DhaR and is displaced by DhaL-ADP, which stimulates DhaR activity. In the absence of dihydroxyacetone, DhaL-ADP is converted by the PTS to DhaL-ATP, which does not bind to DhaR.1 Publication

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer. DhaR forms complexes with DhaK and DhaL-ADP.

1 Publication

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
4261628, 15 interactors
850110, 1 interactor

Database of interacting proteins

More...
DIPi
DIP-9438N

Protein interaction database and analysis system

More...
IntActi
P76016, 2 interactors

STRING: functional protein association networks

More...
STRINGi
511145.b1201

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1639
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P76016

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini52 – 189GAF1 PublicationAdd BLAST138
Domaini203 – 265PAS1 PublicationAdd BLAST63
Domaini327 – 552Sigma-54 factor interactionPROSITE-ProRule annotationAdd BLAST226

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 318Sensor domain1 PublicationAdd BLAST318

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4108JU9 Bacteria
COG3284 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_000445_8_12_6

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P76016

KEGG Orthology (KO)

More...
KOi
K05880

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P76016

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00130 PAS, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.30.450.40, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR003593 AAA+_ATPase
IPR029016 GAF-like_dom_sf
IPR009057 Homeobox-like_sf
IPR002197 HTH_Fis
IPR027417 P-loop_NTPase
IPR000014 PAS
IPR035965 PAS-like_dom_sf
IPR013767 PAS_fold
IPR002078 Sigma_54_int
IPR025662 Sigma_54_int_dom_ATP-bd_1
IPR025943 Sigma_54_int_dom_ATP-bd_2

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF02954 HTH_8, 1 hit
PF00989 PAS, 1 hit
PF00158 Sigma54_activat, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00382 AAA, 1 hit
SM00091 PAS, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF46689 SSF46689, 1 hit
SSF52540 SSF52540, 1 hit
SSF55785 SSF55785, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00675 SIGMA54_INTERACT_1, 1 hit
PS00676 SIGMA54_INTERACT_2, 1 hit
PS50045 SIGMA54_INTERACT_4, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P76016-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSGAFNNDGR GISPLIATSW ERCNKLMKRE TWNVPHQAQG VTFASIYRRK
60 70 80 90 100
KAMLTLGQAA LEDAWEYMAP RECALFILDE TACILSRNGD PQTLQQLSAL
110 120 130 140 150
GFNDGTYCAE GIIGTCALSL AAISGQAVKT MADQHFKQVL WNWAFCATPL
160 170 180 190 200
FDSKGRLTGT IALACPVEQT TAADLPLTLA IAREVGNLLL TDSLLAETNR
210 220 230 240 250
HLNQLNALLE SMDDGVISWD EQGNLQFINA QAARVLRLDA TASQGRAITE
260 270 280 290 300
LLTLPAVLQQ AIKQAHPLKH VEATFESQHQ FIDAVITLKP IIETQGTSFI
310 320 330 340 350
LLLHPVEQMR QLMTSQLGKV SHTFAHMPQD DPQTRRLIHF GRQAARSSFP
360 370 380 390 400
VLLCGEEGVG KALLSQAIHN ESERAAGPYI AVNCELYGDA ALAEEFIGGD
410 420 430 440 450
RTDNENGRLS RLELAHGGTL FLEKIEYLAV ELQSALLQVI KQGVITRLDA
460 470 480 490 500
RRLIPIDVKV IATTTADLAM LVEQNRFSRQ LYYALHAFEI TIPPLRMRRG
510 520 530 540 550
SIPALVNNKL RSLEKRFSTR LKIDDDALAR LVSCAWPGND FELYSVIENL
560 570 580 590 600
ALSSDNGRIR VSDLPEHLFT EQATDDVSAT RLSTSLSFAE VEKEAIINAA
610 620 630
QVTGGRIQEM SALLGIGRTT LWRKMKQHGI DAGQFKRRV
Length:639
Mass (Da):70,562
Last modified:December 1, 2000 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i2DD327F3710E1CA7
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74285.2
AP009048 Genomic DNA Translation: BAA36058.2

Protein sequence database of the Protein Information Resource

More...
PIRi
F64866

NCBI Reference Sequences

More...
RefSeqi
NP_415719.2, NC_000913.3
WP_001350505.1, NZ_SSZK01000010.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC74285; AAC74285; b1201
BAA36058; BAA36058; BAA36058

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
945743

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW5188
eco:b1201

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.1084

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74285.2
AP009048 Genomic DNA Translation: BAA36058.2
PIRiF64866
RefSeqiNP_415719.2, NC_000913.3
WP_001350505.1, NZ_SSZK01000010.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LRXX-ray3.25C/D1-318[»]
4LRYX-ray2.83C/D1-318[»]
4LRZX-ray2.32E/F/G/H1-318[»]
SMRiP76016
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi4261628, 15 interactors
850110, 1 interactor
DIPiDIP-9438N
IntActiP76016, 2 interactors
STRINGi511145.b1201

Proteomic databases

PaxDbiP76016
PRIDEiP76016

Genome annotation databases

EnsemblBacteriaiAAC74285; AAC74285; b1201
BAA36058; BAA36058; BAA36058
GeneIDi945743
KEGGiecj:JW5188
eco:b1201
PATRICifig|1411691.4.peg.1084

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB3661

Phylogenomic databases

eggNOGiENOG4108JU9 Bacteria
COG3284 LUCA
HOGENOMiCLU_000445_8_12_6
InParanoidiP76016
KOiK05880
PhylomeDBiP76016

Enzyme and pathway databases

BioCyciEcoCyc:G6628-MONOMER
ECOL316407:JW5188-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P76016

Family and domain databases

CDDicd00130 PAS, 1 hit
Gene3Di3.30.450.40, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR029016 GAF-like_dom_sf
IPR009057 Homeobox-like_sf
IPR002197 HTH_Fis
IPR027417 P-loop_NTPase
IPR000014 PAS
IPR035965 PAS-like_dom_sf
IPR013767 PAS_fold
IPR002078 Sigma_54_int
IPR025662 Sigma_54_int_dom_ATP-bd_1
IPR025943 Sigma_54_int_dom_ATP-bd_2
PfamiView protein in Pfam
PF02954 HTH_8, 1 hit
PF00989 PAS, 1 hit
PF00158 Sigma54_activat, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00091 PAS, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
SSF52540 SSF52540, 1 hit
SSF55785 SSF55785, 1 hit
PROSITEiView protein in PROSITE
PS00675 SIGMA54_INTERACT_1, 1 hit
PS00676 SIGMA54_INTERACT_2, 1 hit
PS50045 SIGMA54_INTERACT_4, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiDHAR_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P76016
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: June 17, 2020
This is version 155 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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