UniProtKB - P75966 (RLUE_ECOLI)
Protein
Ribosomal large subunit pseudouridine synthase E
Gene
rluE
Organism
Escherichia coli (strain K12)
Status
Functioni
Responsible for synthesis of pseudouridine from uracil-2457 in 23S ribosomal RNA.1 Publication
Catalytic activityi
- EC:5.4.99.201 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 79 | Nucleophile1 Publication | 1 |
GO - Molecular functioni
- pseudouridine synthase activity Source: EcoCyc
- RNA binding Source: InterPro
- rRNA pseudouridine synthase activity Source: EcoCyc
GO - Biological processi
- enzyme-directed rRNA pseudouridine synthesis Source: EcoCyc
Keywordsi
Molecular function | Isomerase |
Biological process | rRNA processing |
Enzyme and pathway databases
BioCyci | EcoCyc:G6581-MONOMER MetaCyc:G6581-MONOMER |
BRENDAi | 5.4.99.20, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Ribosomal large subunit pseudouridine synthase E (EC:5.4.99.20)Alternative name(s): rRNA pseudouridylate synthase E rRNA-uridine isomerase E |
Gene namesi | Name:rluE Synonyms:ymfC Ordered Locus Names:b1135, JW1121 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 79 | D → N or T: Loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000100001 | 1 – 217 | Ribosomal large subunit pseudouridine synthase EAdd BLAST | 217 |
Proteomic databases
jPOSTi | P75966 |
PaxDbi | P75966 |
PRIDEi | P75966 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4263127, 32 interactors 850073, 1 interactor |
DIPi | DIP-12716N |
IntActi | P75966, 8 interactors |
STRINGi | 511145.b1135 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P75966 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P75966 |
Family & Domainsi
Sequence similaritiesi
Belongs to the pseudouridine synthase RsuA family.Curated
Phylogenomic databases
eggNOGi | COG1187, Bacteria |
HOGENOMi | CLU_024979_8_0_6 |
InParanoidi | P75966 |
PhylomeDBi | P75966 |
Family and domain databases
Gene3Di | 3.30.70.1560, 1 hit |
InterProi | View protein in InterPro IPR042092, PsdUridine_s_RsuA/RluB/E/F_cat IPR020103, PsdUridine_synth_cat_dom_sf IPR006145, PsdUridine_synth_RsuA/RluA IPR000748, PsdUridine_synth_RsuA/RluB/E/F IPR018496, PsdUridine_synth_RsuA/RluB_CS |
Pfami | View protein in Pfam PF00849, PseudoU_synth_2, 1 hit |
SUPFAMi | SSF55120, SSF55120, 1 hit |
TIGRFAMsi | TIGR00093, TIGR00093, 1 hit |
PROSITEi | View protein in PROSITE PS01149, PSI_RSU, 1 hit |
i Sequence
Sequence statusi: Complete.
P75966-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRQFIISENT MQKTSFRNHQ VKRFSSQRST RRKPENQPTR VILFNKPYDV
60 70 80 90 100
LPQFTDEAGR KTLKEFIPVQ GVYAAGRLDR DSEGLLVLTN NGALQARLTQ
110 120 130 140 150
PGKRTGKIYY VQVEGIPTQD ALEALRNGVT LNDGPTLPAG AELVDEPAWL
160 170 180 190 200
WPRNPPIRER KSIPTSWLKI TLYEGRNRQV RRMTAHVGFP TLRLIRYAMG
210
DYSLDNLANG EWREVTD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74219.2 AP009048 Genomic DNA Translation: BAA35957.1 |
PIRi | D64858 |
RefSeqi | NP_415653.4, NC_000913.3 WP_001248691.1, NZ_STEB01000016.1 |
Genome annotation databases
EnsemblBacteriai | AAC74219; AAC74219; b1135 BAA35957; BAA35957; BAA35957 |
GeneIDi | 945701 |
KEGGi | ecj:JW1121 eco:b1135 |
PATRICi | fig|1411691.4.peg.1131 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74219.2 AP009048 Genomic DNA Translation: BAA35957.1 |
PIRi | D64858 |
RefSeqi | NP_415653.4, NC_000913.3 WP_001248691.1, NZ_STEB01000016.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2OLW | X-ray | 1.60 | A/B | 1-217 | [»] | |
2OML | X-ray | 1.20 | A | 29-217 | [»] | |
SMRi | P75966 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263127, 32 interactors 850073, 1 interactor |
DIPi | DIP-12716N |
IntActi | P75966, 8 interactors |
STRINGi | 511145.b1135 |
Proteomic databases
jPOSTi | P75966 |
PaxDbi | P75966 |
PRIDEi | P75966 |
Genome annotation databases
EnsemblBacteriai | AAC74219; AAC74219; b1135 BAA35957; BAA35957; BAA35957 |
GeneIDi | 945701 |
KEGGi | ecj:JW1121 eco:b1135 |
PATRICi | fig|1411691.4.peg.1131 |
Organism-specific databases
EchoBASEi | EB3221 |
Phylogenomic databases
eggNOGi | COG1187, Bacteria |
HOGENOMi | CLU_024979_8_0_6 |
InParanoidi | P75966 |
PhylomeDBi | P75966 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6581-MONOMER MetaCyc:G6581-MONOMER |
BRENDAi | 5.4.99.20, 2026 |
Miscellaneous databases
EvolutionaryTracei | P75966 |
PROi | PR:P75966 |
Family and domain databases
Gene3Di | 3.30.70.1560, 1 hit |
InterProi | View protein in InterPro IPR042092, PsdUridine_s_RsuA/RluB/E/F_cat IPR020103, PsdUridine_synth_cat_dom_sf IPR006145, PsdUridine_synth_RsuA/RluA IPR000748, PsdUridine_synth_RsuA/RluB/E/F IPR018496, PsdUridine_synth_RsuA/RluB_CS |
Pfami | View protein in Pfam PF00849, PseudoU_synth_2, 1 hit |
SUPFAMi | SSF55120, SSF55120, 1 hit |
TIGRFAMsi | TIGR00093, TIGR00093, 1 hit |
PROSITEi | View protein in PROSITE PS01149, PSI_RSU, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RLUE_ECOLI | |
Accessioni | P75966Primary (citable) accession number: P75966 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | December 15, 1998 | |
Last modified: | December 2, 2020 | |
This is version 147 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families