UniProtKB - P75954 (YCFS_ECOLI)
Protein
Probable L,D-transpeptidase YcfS
Gene
ycfS
Organism
Escherichia coli (strain K12)
Status
Functioni
Responsible, at least in part, for anchoring of the major outer membrane lipoprotein (Lpp, also known as the Braun lipoprotein) to the peptidoglycan via a meso-diaminopimelyl-L-Lys- bond on the terminal residue of Lpp.1 Publication
: peptidoglycan biosynthesis Pathwayi
This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 217 | Sequence analysis | 1 |
GO - Molecular functioni
- peptidoglycan L,D-transpeptidase activity Source: EcoCyc
- transferase activity, transferring glycosyl groups Source: UniProtKB-KW
GO - Biological processi
- cell wall organization Source: UniProtKB-KW
- peptidoglycan-protein cross-linking Source: EcoCyc
- regulation of cell shape Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosyltransferase, Hydrolase, Transferase |
Biological process | Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:G6571-MONOMER |
UniPathwayi | UPA00219 |
Protein family/group databases
MEROPSi | C82.A05 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ycfS Ordered Locus Names:b1113, JW5820 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Other locations
- Periplasm Curated
Other locations
- periplasmic space Source: UniProtKB-SubCell
Keywords - Cellular componenti
PeriplasmPathology & Biotechi
Disruption phenotypei
Simultaneous disruption of erfK, ybiS, ycfS and ynhG leads to loss of covalent anchoring of the major outer membrane lipoprotein (Lpp) to the peptidoglycan. Complementation with ycfS restores some of this anchoring.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | Sequence analysisAdd BLAST | 23 | |
ChainiPRO_0000013826 | 24 – 320 | Probable L,D-transpeptidase YcfSAdd BLAST | 297 |
Proteomic databases
PaxDbi | P75954 |
PRIDEi | P75954 |
Interactioni
Subunit structurei
Interacts with DsbG.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4260085, 12 interactors |
DIPi | DIP-11546N |
IntActi | P75954, 2 interactors |
STRINGi | 511145.b1113 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 45 – 90 | LysMPROSITE-ProRule annotationAdd BLAST | 46 |
Sequence similaritiesi
Belongs to the YkuD family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG1376, Bacteria COG1388, Bacteria |
HOGENOMi | CLU_046834_0_0_6 |
InParanoidi | P75954 |
PhylomeDBi | P75954 |
Family and domain databases
CDDi | cd00118, LysM, 1 hit cd16913, YkuD_like, 1 hit |
Gene3Di | 2.40.440.10, 1 hit |
InterProi | View protein in InterPro IPR005490, LD_TPept_cat_dom IPR041597, Ldt_C IPR018392, LysM_dom IPR038063, Transpep_catalytic_dom |
Pfami | View protein in Pfam PF17969, Ldt_C, 1 hit PF01476, LysM, 1 hit PF03734, YkuD, 1 hit |
SMARTi | View protein in SMART SM00257, LysM, 1 hit |
SUPFAMi | SSF141523, SSF141523, 1 hit |
PROSITEi | View protein in PROSITE PS51782, LYSM, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P75954-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMIKTRFSRW LTFFTFAAAV ALALPAKANT WPLPPAGSRL VGENKFHVVE
60 70 80 90 100
NDGGSLEAIA KKYNVGFLAL LQANPGVDPY VPRAGSVLTI PLQTLLPDAP
110 120 130 140 150
REGIVINIAE LRLYYYPPGK NSVTVYPIGI GQLGGDTLTP TMVTTVSDKR
160 170 180 190 200
ANPTWTPTAN IRARYKAQGI ELPAVVPAGL DNPMGHHAIR LAAYGGVYLL
210 220 230 240 250
HGTNADFGIG MRVSSGCIRL RDDDIKTLFS QVTPGTKVNI INTPIKVSAE
260 270 280 290 300
PNGARLVEVH QPLSEKIDDD PQLLPITLNS AMQSFKDAAQ TDAEVMQHVM
310 320
DVRSGMPVDV RRHQVSPQTL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74197.1 AP009048 Genomic DNA Translation: BAA35928.1 |
PIRi | F64855 |
RefSeqi | NP_415631.1, NC_000913.3 |
Genome annotation databases
EnsemblBacteriai | AAC74197; AAC74197; b1113 BAA35928; BAA35928; BAA35928 |
GeneIDi | 945666 |
KEGGi | ecj:JW5820 eco:b1113 |
PATRICi | fig|511145.12.peg.1157 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74197.1 AP009048 Genomic DNA Translation: BAA35928.1 |
PIRi | F64855 |
RefSeqi | NP_415631.1, NC_000913.3 |
3D structure databases
SMRi | P75954 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260085, 12 interactors |
DIPi | DIP-11546N |
IntActi | P75954, 2 interactors |
STRINGi | 511145.b1113 |
Protein family/group databases
MEROPSi | C82.A05 |
Proteomic databases
PaxDbi | P75954 |
PRIDEi | P75954 |
Genome annotation databases
EnsemblBacteriai | AAC74197; AAC74197; b1113 BAA35928; BAA35928; BAA35928 |
GeneIDi | 945666 |
KEGGi | ecj:JW5820 eco:b1113 |
PATRICi | fig|511145.12.peg.1157 |
Organism-specific databases
EchoBASEi | EB3211 |
Phylogenomic databases
eggNOGi | COG1376, Bacteria COG1388, Bacteria |
HOGENOMi | CLU_046834_0_0_6 |
InParanoidi | P75954 |
PhylomeDBi | P75954 |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
BioCyci | EcoCyc:G6571-MONOMER |
Miscellaneous databases
PROi | PR:P75954 |
Family and domain databases
CDDi | cd00118, LysM, 1 hit cd16913, YkuD_like, 1 hit |
Gene3Di | 2.40.440.10, 1 hit |
InterProi | View protein in InterPro IPR005490, LD_TPept_cat_dom IPR041597, Ldt_C IPR018392, LysM_dom IPR038063, Transpep_catalytic_dom |
Pfami | View protein in Pfam PF17969, Ldt_C, 1 hit PF01476, LysM, 1 hit PF03734, YkuD, 1 hit |
SMARTi | View protein in SMART SM00257, LysM, 1 hit |
SUPFAMi | SSF141523, SSF141523, 1 hit |
PROSITEi | View protein in PROSITE PS51782, LYSM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | YCFS_ECOLI | |
Accessioni | P75954Primary (citable) accession number: P75954 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | February 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 143 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families