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Protein

Beta-hexosaminidase

Gene

nagZ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in peptidoglycan recycling by cleaving the terminal beta-1,4-linked N-acetylglucosamine (GlcNAc) from peptide-linked peptidoglycan fragments, giving rise to free GlcNAc, anhydro-N-acetylmuramic acid and anhydro-N-acetylmuramic acid-linked peptides. Cleaves GlcNAc linked beta-1,4 to MurNAc tripeptides.

Catalytic activityi

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.UniRule annotation

Activity regulationi

Slightly inhibited by GlcNAc and N-acetylmuramic acid and strongly inhibited by N-acetylglucosaminolactone.

pH dependencei

Optimum pH is 7.7.

Pathwayi: peptidoglycan recycling

This protein is involved in the pathway peptidoglycan recycling, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan recycling and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei62SubstrateUniRule annotation1
Binding sitei70SubstrateUniRule annotation1
Binding sitei133SubstrateUniRule annotation1
Sitei174Important for catalytic activityUniRule annotation1
Active sitei176Proton donor/acceptorUniRule annotation1
Active sitei248NucleophileUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciEcoCyc:G6567-MONOMER
MetaCyc:G6567-MONOMER
UniPathwayi
UPA00544

Protein family/group databases

CAZyiGH3 Glycoside Hydrolase Family 3

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-hexosaminidaseUniRule annotation (EC:3.2.1.52UniRule annotation)
Alternative name(s):
Beta-N-acetylhexosaminidaseUniRule annotation
N-acetyl-beta-glucosaminidaseUniRule annotation
Gene namesi
Name:nagZUniRule annotation
Synonyms:ycfO
Ordered Locus Names:b1107, JW1093
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13433 nagZ

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002107851 – 341Beta-hexosaminidaseAdd BLAST341

Proteomic databases

PaxDbiP75949
PRIDEiP75949

Interactioni

Subunit structurei

Monomer.Curated

Protein-protein interaction databases

BioGridi4259534, 56 interactors
IntActiP75949, 8 interactors
STRINGi316385.ECDH10B_1179

Structurei

3D structure databases

ProteinModelPortaliP75949
SMRiP75949
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni163 – 164Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QPA Bacteria
COG1472 LUCA
HOGENOMiHOG000248526
InParanoidiP75949
KOiK01207
OMAiHKETPRD
PhylomeDBiP75949

Family and domain databases

Gene3Di3.20.20.300, 1 hit
HAMAPiMF_00364 NagZ, 1 hit
InterProiView protein in InterPro
IPR022956 Beta_hexosaminidase_bac
IPR019800 Glyco_hydro_3_AS
IPR001764 Glyco_hydro_3_N
IPR036962 Glyco_hydro_3_N_sf
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00933 Glyco_hydro_3, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00775 GLYCOSYL_HYDROL_F3, 1 hit

Sequencei

Sequence statusi: Complete.

P75949-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGPVMLDVEG YELDAEEREI LAHPLVGGLI LFTRNYHDPA QLRELVRQIR
60 70 80 90 100
AASRNRLVVA VDQEGGRVQR FREGFTRLPA AQSFAALSGM EEGGKLAQEA
110 120 130 140 150
GWLMASEMIA MDIDISFAPV LDVGHISAAI GERSYHADPQ KALAIASRFI
160 170 180 190 200
DGMHEAGMKT TGKHFPGHGA VTADSHKETP CDPRPQAEIR AKDMSVFSSL
210 220 230 240 250
IRENKLDAIM PAHVIYSDVD PRPASGSPYW LKTVLRQELG FDGVIFSDDL
260 270 280 290 300
SMEGAAIMGS YAERGQASLD AGCDMILVCN NRKGAVSVLD NLSPIKAERV
310 320 330 340
TRLYHKGSFS RQELMDSARW KAISTRLNQL HERWQEEKAG H
Length:341
Mass (Da):37,595
Last modified:February 1, 1997 - v1
Checksum:i9E50C8E4DA87C7A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74191.1
AP009048 Genomic DNA Translation: BAA35914.1
PIRiH64854
RefSeqiNP_415625.1, NC_000913.3
WP_000529320.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74191; AAC74191; b1107
BAA35914; BAA35914; BAA35914
GeneIDi945671
KEGGiecj:JW1093
eco:b1107
PATRICifig|1411691.4.peg.1160

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74191.1
AP009048 Genomic DNA Translation: BAA35914.1
PIRiH64854
RefSeqiNP_415625.1, NC_000913.3
WP_000529320.1, NZ_LN832404.1

3D structure databases

ProteinModelPortaliP75949
SMRiP75949
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259534, 56 interactors
IntActiP75949, 8 interactors
STRINGi316385.ECDH10B_1179

Protein family/group databases

CAZyiGH3 Glycoside Hydrolase Family 3

Proteomic databases

PaxDbiP75949
PRIDEiP75949

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74191; AAC74191; b1107
BAA35914; BAA35914; BAA35914
GeneIDi945671
KEGGiecj:JW1093
eco:b1107
PATRICifig|1411691.4.peg.1160

Organism-specific databases

EchoBASEiEB3207
EcoGeneiEG13433 nagZ

Phylogenomic databases

eggNOGiENOG4107QPA Bacteria
COG1472 LUCA
HOGENOMiHOG000248526
InParanoidiP75949
KOiK01207
OMAiHKETPRD
PhylomeDBiP75949

Enzyme and pathway databases

UniPathwayi
UPA00544

BioCyciEcoCyc:G6567-MONOMER
MetaCyc:G6567-MONOMER

Miscellaneous databases

PROiPR:P75949

Family and domain databases

Gene3Di3.20.20.300, 1 hit
HAMAPiMF_00364 NagZ, 1 hit
InterProiView protein in InterPro
IPR022956 Beta_hexosaminidase_bac
IPR019800 Glyco_hydro_3_AS
IPR001764 Glyco_hydro_3_N
IPR036962 Glyco_hydro_3_N_sf
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00933 Glyco_hydro_3, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00775 GLYCOSYL_HYDROL_F3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNAGZ_ECOLI
AccessioniPrimary (citable) accession number: P75949
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: September 12, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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