flgJ

Functionⁱ

Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space.By similarity

Miscellaneous

Probably exported via the flagellum-specific export pathway.

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	220	Sequence analysis		1
Active siteⁱ	245	Sequence analysis		1

GO - Molecular functionⁱ

amidase activity Source: InterPro
hydrolase activity, acting on glycosyl bonds Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

bacterial-type flagellum assembly Source: InterPro
bacterial-type flagellum-dependent cell motility
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 25816013
cell wall organization Source: UniProtKB-KW
metabolic process Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Glycosidase, Hydrolase
Biological process	Bacterial flagellum biogenesis, Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCycⁱ	EcoCyc:G366-MONOMER

BioCycⁱ

EcoCyc:G366-MONOMER

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	GH73 Glycoside Hydrolase Family 73

CAZyⁱ

GH73 Glycoside Hydrolase Family 73

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Peptidoglycan hydrolase FlgJ (EC:3.2.1.-) Alternative name(s): Muramidase FlgJ
Gene namesⁱ	Name:flgJ Synonyms:fla FX, flaZ Ordered Locus Names:b1081, JW1068
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG14272 flgJ

EcoGeneⁱ

EG14272 flgJ

Subcellular locationⁱ

Periplasm By similarity

GO - Cellular componentⁱ

periplasmic space Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Periplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000165705	1 – 313	Peptidoglycan hydrolase FlgJAdd BLAST		313

Proteomic databases

PaxDbⁱ	P75942
PRIDEⁱ	P75942

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	4261020, 118 interactors
IntActⁱ	P75942, 11 interactors
STRINGⁱ	316385.ECDH10B_1152

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P75942
SMRⁱ	P75942
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	148 – 313	CatalyticAdd BLAST		166

Sequence similaritiesⁱ

In the N-terminal section; belongs to the FlgJ family.Curated

In the C-terminal section; belongs to the glycosyl hydrolase 73 family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4108ZW7 Bacteria COG1705 LUCA COG3951 LUCA
HOGENOMⁱ	HOG000265620
InParanoidⁱ	P75942
KEGG Orthology (KO) More...KOⁱ	K02395

Family and domain databases

InterProⁱ	View protein in InterPro IPR019301 Flagellar_prot_FlgJ_N IPR013377 FlaJ IPR002901 MGlyc_endo_b_GlcNAc-like_dom
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01832 Glucosaminidase, 1 hit PF10135 Rod-binding, 1 hit
SMARTⁱ	View protein in SMART SM00047 LYZ2, 1 hit
TIGRFAMsⁱ	TIGR02541 flagell_FlgJ, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P75942-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MISDSKLLAS AAWDAQSLNE LKAKAGEDPA ANIRPVARQV EGMFVQMMLK 
        60         70         80         90        100
SMRDALPKDG LFSSEHTRLY TSMYDQQIAQ QMTAGKGLGL AEMMVKQMTP 
       110        120        130        140        150
EQPLPEESTP AAPMKFPLET VVRYQNQALS QLVQKAVPRN YDDSLPGDSK 
       160        170        180        190        200
AFLAQLSLPA QLASQQSGVP HHLILAQAAL ESGWGQRQIR RENGEPSYNL 
       210        220        230        240        250
FGVKASGNWK GPVTEITTTE YENGEAKKVK AKFRVYSSYL EALSDYVGLL 
       260        270        280        290        300
TRNPRYAAVT TAASAEQGAQ ALQDAGYATD PHYARKLTNM IQQMKSISDK 
       310 
VSKTYSMNID NLF

Length:313

Mass (Da):34,475

Last modified:February 1, 1997 - v1

Checksum:ⁱC4B3CB8F36751CDF

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U00096 Genomic DNA Translation: AAC74165.1 AP009048 Genomic DNA Translation: BAA35890.1
PIRⁱ	F64851
NCBI Reference Sequences More...RefSeqⁱ	NP_415599.1, NC_000913.3 WP_001295441.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC74165; AAC74165; b1081 BAA35890; BAA35890; BAA35890
GeneIDⁱ	947456
KEGGⁱ	ecj:JW1068 eco:b1081
PATRICⁱ	fig\|1411691.4.peg.1187

Protein	Similar proteins		Species	Score	Length	Source
P75942	Flagellar protein FlgJ		Escherichia coli G3/10		323	UniRef100_P75942
Flagellar rod assembly protein/muramidase FlgJ (Fragment)		uncultured bacterium Contig651		74
Flagellar rod assembly protein/muramidase FlgJ		Escherichia coli H386		323
Flagellar rod assembly protein/muramidase FlgJ		Escherichia coli H591		323
Flagellar rod assembly protein/muramidase FlgJ		Escherichia coli H736		323
+85

Protein	Similar proteins		Species	Score	Length	Source
P75942	Flagellar protein FlgJ		Escherichia coli G3/10		323	UniRef90_P75942
Flagellar rod assembly protein/muramidase FlgJ (Fragment)		uncultured bacterium Contig651		74
Flagellar rod assembly protein/muramidase FlgJ		Escherichia coli H386		323
Flagellar rod assembly protein/muramidase FlgJ		Escherichia coli H591		323
Flagellar rod assembly protein/muramidase FlgJ		Escherichia coli H736		323
+85

Protein	Similar proteins		Species	Score	Length	Source
P75942	Flagellar protein FlgJ		Escherichia coli G3/10		323	UniRef50_P75942
Flagellar rod assembly protein/muramidase FlgJ (Fragment)		uncultured bacterium Contig651		74
Flagellar rod assembly protein/muramidase FlgJ		Escherichia coli H386		323
Flagellar rod assembly protein/muramidase FlgJ		Escherichia coli H591		323
Flagellar rod assembly protein/muramidase FlgJ		Escherichia coli H736		323
+114

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U00096 Genomic DNA Translation: AAC74165.1 AP009048 Genomic DNA Translation: BAA35890.1
PIRⁱ	F64851
RefSeqⁱ	NP_415599.1, NC_000913.3 WP_001295441.1, NZ_LN832404.1

3D structure databases

ProteinModelPortalⁱ	P75942
SMRⁱ	P75942
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4261020, 118 interactors
IntActⁱ	P75942, 11 interactors
STRINGⁱ	316385.ECDH10B_1152

Protein family/group databases

CAZyⁱ	GH73 Glycoside Hydrolase Family 73

CAZyⁱ

GH73 Glycoside Hydrolase Family 73

Proteomic databases

PaxDbⁱ	P75942
PRIDEⁱ	P75942

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC74165; AAC74165; b1081 BAA35890; BAA35890; BAA35890
GeneIDⁱ	947456
KEGGⁱ	ecj:JW1068 eco:b1081
PATRICⁱ	fig\|1411691.4.peg.1187

Organism-specific databases

EchoBASEⁱ	EB4020
EcoGeneⁱ	EG14272 flgJ

Phylogenomic databases

eggNOGⁱ	ENOG4108ZW7 Bacteria COG1705 LUCA COG3951 LUCA
HOGENOMⁱ	HOG000265620
InParanoidⁱ	P75942
KOⁱ	K02395

Enzyme and pathway databases

BioCycⁱ	EcoCyc:G366-MONOMER

BioCycⁱ

EcoCyc:G366-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P75942

PROⁱ

PR:P75942

Family and domain databases

InterProⁱ	View protein in InterPro IPR019301 Flagellar_prot_FlgJ_N IPR013377 FlaJ IPR002901 MGlyc_endo_b_GlcNAc-like_dom
Pfamⁱ	View protein in Pfam PF01832 Glucosaminidase, 1 hit PF10135 Rod-binding, 1 hit
SMARTⁱ	View protein in SMART SM00047 LYZ2, 1 hit
TIGRFAMsⁱ	TIGR02541 flagell_FlgJ, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	FLGJ_ECOLI
Accessionⁱ	P75942Primary (citable) accession number: P75942
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
	Last sequence update:	February 1, 1997
	Last modified:	November 7, 2018
	This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P75942 (FLGJ_ECOLI)

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Peptidoglycan hydrolase FlgJ

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Sites

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GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ