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Protein

Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase

Gene

pgaB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide. N-deacetylation promotes PGA export through the PgaA porin.2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyciEcoCyc:G6530-MONOMER
MetaCyc:G6530-MONOMER
BRENDAi3.1.1.58 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase (EC:3.5.1.-)
Short name:
PGA N-deacetylase
Short name:
Poly-beta-1,6-GlcNAc N-deacetylase
Gene namesi
Name:pgaB
Synonyms:ycdR
Ordered Locus Names:b1023, JW5142
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13864 pgaB

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion of pgaB does not prevent PGA synthesis but does block its export and biofilm formation. The synthesized PGA is retained in the periplasm and accumulates at the cell poles.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi115D → A: High decrease in catalytic activity. Unable to support biofilm formation and PGA secretion. 1 Publication1
Mutagenesisi184H → A: Unable to support biofilm formation and PGA secretion. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20PROSITE-ProRule annotationAdd BLAST20
ChainiPRO_000002484421 – 672Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylaseAdd BLAST652

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi21N-palmitoyl cysteinePROSITE-ProRule annotation1
Lipidationi21S-diacylglycerol cysteinePROSITE-ProRule annotation1

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiP75906
PRIDEiP75906

Interactioni

Protein-protein interaction databases

BioGridi4260050, 16 interactors
DIPiDIP-11513N
STRINGi316385.ECDH10B_1096

Chemistry databases

BindingDBiP75906

Structurei

Secondary structure

1672
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP75906
SMRiP75906
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini107 – 349NodB homologyPROSITE-ProRule annotationAdd BLAST243

Domaini

Contains a N-terminal polysaccharide deacetylase domain, and a C-terminal domain required for PGA N-deacetylation that may be involved in binding to unmodified poly-beta-1,6-GlcNAc and thereby assists catalysis by the deacetylase domain.1 Publication

Sequence similaritiesi

Belongs to the polysaccharide deacetylase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107BJW Bacteria
COG0726 LUCA
HOGENOMiHOG000170240
KOiK11931

Family and domain databases

InterProiView protein in InterPro
IPR011330 Glyco_hydro/deAcase_b/a-brl
IPR002509 NODB_dom
IPR023854 PGA_deacetylase_PgaB
IPR032772 PGA_deacetylase_PgaB_C
PfamiView protein in Pfam
PF14883 GHL13, 1 hit
PF01522 Polysacc_deac_1, 1 hit
SUPFAMiSSF88713 SSF88713, 1 hit
TIGRFAMsiTIGR03938 deacetyl_PgaB, 1 hit
PROSITEiView protein in PROSITE
PS51677 NODB, 1 hit
PS51257 PROKAR_LIPOPROTEIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P75906-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLRNGNKYLL MLVSIIMLTA CISQSRTSFI PPQDRESLLA EQPWPHNGFV
60 70 80 90 100
AISWHNVEDE AADQRFMSVR TSALREQFAW LRENGYQPVS IAQIREAHRG
110 120 130 140 150
GKPLPEKAVV LTFDDGYQSF YTRVFPILQA FQWPAVWAPV GSWVDTPADK
160 170 180 190 200
QVKFGDELVD REYFATWQQV REVARSRLVE LASHTWNSHY GIQANATGSL
210 220 230 240 250
LPVYVNRAYF TDHARYETAA EYRERIRLDA VKMTEYLRTK VEVNPHVFVW
260 270 280 290 300
PYGEANGIAI EELKKLGYDM FFTLESGLAN ASQLDSIPRV LIANNPSLKE
310 320 330 340 350
FAQQIITVQE KSPQRIMHID LDYVYDENLQ QMDRNIDVLI QRVKDMQIST
360 370 380 390 400
VYLQAFADPD GDGLVKEVWF PNRLLPMKAD IFSRVAWQLR TRSGVNIYAW
410 420 430 440 450
MPVLSWDLDP TLTRVKYLPT GEKKAQIHPE QYHRLSPFDD RVRAQVGMLY
460 470 480 490 500
EDLAGHAAFD GILFHDDALL SDYEDASAPA ITAYQQAGFS GSLSEIRQNP
510 520 530 540 550
EQFKQWARFK SRALTDFTLE LSARVKAIRG PHIKTARNIF ALPVIQPESE
560 570 580 590 600
AWFAQNYADF LKSYDWTAIM AMPYLEGVAE KSADQWLIQL TNQIKNIPQA
610 620 630 640 650
KDKSILELQA QNWQKNGQHQ AISSQQLAHW MSLLQLNGVK NYGYYPDNFL
660 670
HNQPEIDLIR PEFSTAWYPK ND
Length:672
Mass (Da):77,413
Last modified:February 1, 1997 - v1
Checksum:i8B68D0FFB657F4F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74108.1
AP009048 Genomic DNA Translation: BAA35805.2
PIRiE64844
RefSeqiNP_415542.1, NC_000913.3
WP_000945561.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74108; AAC74108; b1023
BAA35805; BAA35805; BAA35805
GeneIDi945604
KEGGiecj:JW5142
eco:b1023
PATRICifig|1411691.4.peg.1246

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74108.1
AP009048 Genomic DNA Translation: BAA35805.2
PIRiE64844
RefSeqiNP_415542.1, NC_000913.3
WP_000945561.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VUSX-ray1.65A/B42-309[»]
4F9DX-ray1.90A/B42-655[»]
4F9JX-ray2.10A/B42-655[»]
4P7LX-ray1.80A310-672[»]
4P7NX-ray1.89A310-672[»]
4P7OX-ray1.48A/B310-672[»]
4P7QX-ray1.65A310-672[»]
4P7RX-ray1.80A310-672[»]
ProteinModelPortaliP75906
SMRiP75906
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260050, 16 interactors
DIPiDIP-11513N
STRINGi316385.ECDH10B_1096

Chemistry databases

BindingDBiP75906

Proteomic databases

PaxDbiP75906
PRIDEiP75906

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74108; AAC74108; b1023
BAA35805; BAA35805; BAA35805
GeneIDi945604
KEGGiecj:JW5142
eco:b1023
PATRICifig|1411691.4.peg.1246

Organism-specific databases

EchoBASEiEB3624
EcoGeneiEG13864 pgaB

Phylogenomic databases

eggNOGiENOG4107BJW Bacteria
COG0726 LUCA
HOGENOMiHOG000170240
KOiK11931

Enzyme and pathway databases

BioCyciEcoCyc:G6530-MONOMER
MetaCyc:G6530-MONOMER
BRENDAi3.1.1.58 2026

Miscellaneous databases

PROiPR:P75906

Family and domain databases

InterProiView protein in InterPro
IPR011330 Glyco_hydro/deAcase_b/a-brl
IPR002509 NODB_dom
IPR023854 PGA_deacetylase_PgaB
IPR032772 PGA_deacetylase_PgaB_C
PfamiView protein in Pfam
PF14883 GHL13, 1 hit
PF01522 Polysacc_deac_1, 1 hit
SUPFAMiSSF88713 SSF88713, 1 hit
TIGRFAMsiTIGR03938 deacetyl_PgaB, 1 hit
PROSITEiView protein in PROSITE
PS51677 NODB, 1 hit
PS51257 PROKAR_LIPOPROTEIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPGAB_ECOLI
AccessioniPrimary (citable) accession number: P75906
Secondary accession number(s): Q9R7P5, Q9R7P7, Q9R7P8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 7, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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