UniProtKB - P75897 (RUTB_ECOLI)
Protein
Ureidoacrylate amidohydrolase RutB
Gene
rutB
Organism
Escherichia coli (strain K12)
Status
Functioni
Hydrolyzes ureidoacrylate to form aminoacrylate and carbamate. The carbamate hydrolyzes spontaneously, thereby releasing one of the nitrogen atoms of the pyrimidine ring as ammonia and one of its carbon atoms as CO2.2 Publications
Miscellaneous
The Rut pathway degrades exogenous pyrimidines as the sole nitrogen source at room temperature but not at 37 degrees Celsius, a restriction that is apparently a consequence of an inadequate ability to remove toxic malonic semialdehyde at the higher temperature (RutE/YdfG function).
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 166 | NucleophileBy similarity | 1 |
GO - Molecular functioni
- hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides Source: UniProtKB
GO - Biological processi
- nitrogen utilization Source: UniProtKB
- pyrimidine nucleobase catabolic process Source: EcoCyc
- uracil catabolic process Source: UniProtKB
Keywordsi
Molecular function | Hydrolase |
Enzyme and pathway databases
BioCyci | EcoCyc:G6522-MONOMER MetaCyc:G6522-MONOMER |
BRENDAi | 3.5.1.110, 2026 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:rutB Synonyms:ycdL Ordered Locus Names:b1011, JW5139 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Cells lacking this gene fail to grow on uridine as the sole source of nitrogen at room temperature.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000201833 | 1 – 230 | Ureidoacrylate amidohydrolase RutBAdd BLAST | 230 |
Proteomic databases
PaxDbi | P75897 |
PRIDEi | P75897 |
Expressioni
Inductioni
Up-regulated by the nitrogen regulatory protein C (NtrC also called GlnG) and repressed by RutR.2 Publications
Interactioni
Protein-protein interaction databases
BioGRIDi | 4261997, 1 interactor |
DIPi | DIP-28106N |
STRINGi | 511145.b1011 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1335, Bacteria |
InParanoidi | P75897 |
PhylomeDBi | P75897 |
Family and domain databases
Gene3Di | 3.40.50.850, 1 hit |
HAMAPi | MF_00830, RutB, 1 hit |
InterProi | View protein in InterPro IPR000868, Isochorismatase-like IPR036380, Isochorismatase-like_sf IPR019916, RutB |
Pfami | View protein in Pfam PF00857, Isochorismatase, 1 hit |
SUPFAMi | SSF52499, SSF52499, 1 hit |
TIGRFAMsi | TIGR03614, RutB, 1 hit |
i Sequence
Sequence statusi: Complete.
P75897-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTTLTARPEA ITFDPQQSAL IVVDMQNAYA TPGGYLDLAG FDVSTTRPVI
60 70 80 90 100
ANIQTAVTAA RAAGMLIIWF QNGWDEQYVE AGGPGSPNFH KSNALKTMRK
110 120 130 140 150
QPQLQGKLLA KGSWDYQLVD ELVPQPGDIV LPKPRYSGFF NTPLDSILRS
160 170 180 190 200
RGIRHLVFTG IATNVCVEST LRDGFFLEYF GVVLEDATHQ AGPKFAQKAA
210 220 230
LFNIETFFGW VSDVETFCDA LSPTSFAHIA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74096.2 AP009048 Genomic DNA Translation: BAA35778.1 |
PIRi | A64843 |
RefSeqi | NP_415531.2, NC_000913.3 WP_001393558.1, NZ_SSZK01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAC74096; AAC74096; b1011 BAA35778; BAA35778; BAA35778 |
GeneIDi | 945699 |
KEGGi | ecj:JW5139 eco:b1011 |
PATRICi | fig|511145.12.peg.1049 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74096.2 AP009048 Genomic DNA Translation: BAA35778.1 |
PIRi | A64843 |
RefSeqi | NP_415531.2, NC_000913.3 WP_001393558.1, NZ_SSZK01000002.1 |
3D structure databases
SMRi | P75897 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261997, 1 interactor |
DIPi | DIP-28106N |
STRINGi | 511145.b1011 |
Proteomic databases
PaxDbi | P75897 |
PRIDEi | P75897 |
Genome annotation databases
EnsemblBacteriai | AAC74096; AAC74096; b1011 BAA35778; BAA35778; BAA35778 |
GeneIDi | 945699 |
KEGGi | ecj:JW5139 eco:b1011 |
PATRICi | fig|511145.12.peg.1049 |
Organism-specific databases
EchoBASEi | EB3618 |
Phylogenomic databases
eggNOGi | COG1335, Bacteria |
InParanoidi | P75897 |
PhylomeDBi | P75897 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6522-MONOMER MetaCyc:G6522-MONOMER |
BRENDAi | 3.5.1.110, 2026 |
Miscellaneous databases
PROi | PR:P75897 |
Family and domain databases
Gene3Di | 3.40.50.850, 1 hit |
HAMAPi | MF_00830, RutB, 1 hit |
InterProi | View protein in InterPro IPR000868, Isochorismatase-like IPR036380, Isochorismatase-like_sf IPR019916, RutB |
Pfami | View protein in Pfam PF00857, Isochorismatase, 1 hit |
SUPFAMi | SSF52499, SSF52499, 1 hit |
TIGRFAMsi | TIGR03614, RutB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RUTB_ECOLI | |
Accessioni | P75897Primary (citable) accession number: P75897 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 27, 2002 |
Last sequence update: | March 27, 2002 | |
Last modified: | December 2, 2020 | |
This is version 134 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families