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Protein

Ribosomal RNA large subunit methyltransferase K/L

Gene

rlmL

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specifically methylates the guanine in position 2445 (m2G2445) and the guanine in position 2069 (m7G2069) of 23S rRNA. Methylation occurs before assembly of 23S rRNA into 50S subunits.3 Publications

Catalytic activityi

S-adenosyl-L-methionine + guanine(2445) in 23S rRNA = S-adenosyl-L-homocysteine + N2-methylguanine(2445) in 23S rRNA.
S-adenosyl-L-methionine + guanine(2069) in 23S rRNA = S-adenosyl-L-homocysteine + N7-methylguanine(2069) in 23S rRNA.

Activity regulationi

The N-terminal RlmL activity is enhanced by the C-terminal RlmK activity.1 Publication

GO - Molecular functioni

GO - Biological processi

  • rRNA base methylation Source: EcoCyc
  • rRNA methylation Source: UniProtKB

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase
Biological processrRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:G6488-MONOMER
MetaCyc:G6488-MONOMER
BRENDAi2.1.1.173 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA large subunit methyltransferase K/L
Including the following 2 domains:
23S rRNA m2G2445 methyltransferase (EC:2.1.1.173)
Alternative name(s):
rRNA (guanine-N(2)-)-methyltransferase RlmL
23S rRNA m7G2069 methyltransferase (EC:2.1.1.264)
Alternative name(s):
rRNA (guanine-N(7)-)-methyltransferase RlmK
Gene namesi
Name:rlmL
Synonyms:rlmK, rlmKL, ycbY
Ordered Locus Names:b0948, JW0931
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13717 rlmL

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: GO_Central

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi195D → A: Does not affect methyltransferase activity. 1 Publication1
Mutagenesisi309N → A: Impairs m2G2445 formation, but does not affect m7G2069 formation. 1 Publication1
Mutagenesisi397N → A: Impairs m2G2445 formation, but does not affect m7G2069 formation. 1 Publication1
Mutagenesisi530R → A: Does not affect methyltransferase activity. 1 Publication1
Mutagenesisi568D → A: Impairs m7G2069 formation, but does not affect m2G2445 formation. 1 Publication1
Mutagenesisi597D → A: Does not affect methyltransferase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001404731 – 702Ribosomal RNA large subunit methyltransferase K/LAdd BLAST702

Proteomic databases

PaxDbiP75864
PRIDEiP75864

Interactioni

Subunit structurei

Monomer.1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi4260025, 148 interactors
DIPiDIP-11486N
IntActiP75864, 48 interactors
STRINGi316385.ECDH10B_1018

Structurei

Secondary structure

1702
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP75864
SMRiP75864
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 154THUMPAdd BLAST112

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 397RlmLAdd BLAST397
Regioni398 – 702RlmKAdd BLAST305

Domaini

Consists of a N-terminal RlmL domain and a C-terminal RlmK domain, linked by a highly flexible loop.2 Publications

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C4H Bacteria
COG0116 LUCA
COG1092 LUCA
HOGENOMiHOG000218371
InParanoidiP75864
KOiK12297
OMAiSWDVQRD
PhylomeDBiP75864

Family and domain databases

HAMAPiMF_01858 23SrRNA_methyltr_KL, 1 hit
InterProiView protein in InterPro
IPR017244 23SrRNA_methyltr_KL
IPR002052 DNA_methylase_N6_adenine_CS
IPR000241 RNA_methylase_dom
IPR019614 SAM-dep_methyl-trfase
IPR029063 SAM-dependent_MTases
IPR004114 THUMP_dom
PfamiView protein in Pfam
PF10672 Methyltrans_SAM, 1 hit
PF02926 THUMP, 1 hit
PF01170 UPF0020, 1 hit
PIRSFiPIRSF037618 RNA_Mtase_bacteria_prd, 1 hit
SMARTiView protein in SMART
SM00981 THUMP, 1 hit
SUPFAMiSSF53335 SSF53335, 3 hits
PROSITEiView protein in PROSITE
PS51165 THUMP, 1 hit
PS01261 UPF0020, 1 hit

Sequencei

Sequence statusi: Complete.

P75864-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNSLFASTAR GLEELLKTEL ENLGAVECQV VQGGVHFKGD TRLVYQSLMW
60 70 80 90 100
SRLASRIMLP LGECKVYSDL DLYLGVQAIN WTEMFNPGAT FAVHFSGLND
110 120 130 140 150
TIRNSQYGAM KVKDAIVDAF TRKNLPRPNV DRDAPDIRVN VWLHKETASI
160 170 180 190 200
ALDLSGDGLH LRGYRDRAGI APIKETLAAA IVMRSGWQPG TPLLDPMCGS
210 220 230 240 250
GTLLIEAAML ATDRAPGLHR GRWGFSGWAQ HDEAIWQEVK AEAQTRARKG
260 270 280 290 300
LAEYSSHFYG SDSDARVIQR ARTNARLAGI GELITFEVKD VAQLTNPLPK
310 320 330 340 350
GPYGTVLSNP PYGERLDSEP ALIALHSLLG RIMKNQFGGW NLSLFSASPD
360 370 380 390 400
LLSCLQLRAD KQYKAKNGPL DCVQKNYHVA ESTPDSKPAM VAEDYTNRLR
410 420 430 440 450
KNLKKFEKWA RQEGIECYRL YDADLPEYNV AVDRYADWVV VQEYAPPKTI
460 470 480 490 500
DAHKARQRLF DIIAATISVL GIAPNKLVLK TRERQKGKNQ YQKLGEKGEF
510 520 530 540 550
LEVTEYNAHL WVNLTDYLDT GLFLDHRIAR RMLGQMSKGK DFLNLFSYTG
560 570 580 590 600
SATVHAGLGG ARSTTTVDMS RTYLEWAERN LRLNGLTGRA HRLIQADCLA
610 620 630 640 650
WLREANEQFD LIFIDPPTFS NSKRMEDAFD VQRDHLALMK DLKRLLRAGG
660 670 680 690 700
TIMFSNNKRG FRMDLDGLAK LGLKAQEITQ KTLSQDFARN RQIHNCWLIT

AA
Length:702
Mass (Da):78,854
Last modified:February 1, 1997 - v1
Checksum:i9EF24AD7A32AC429
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74034.1
AP009048 Genomic DNA Translation: BAA35703.1
PIRiC64835
RefSeqiNP_415468.1, NC_000913.3
WP_001086539.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74034; AAC74034; b0948
BAA35703; BAA35703; BAA35703
GeneIDi945564
KEGGiecj:JW0931
eco:b0948
PATRICifig|1411691.4.peg.1326

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74034.1
AP009048 Genomic DNA Translation: BAA35703.1
PIRiC64835
RefSeqiNP_415468.1, NC_000913.3
WP_001086539.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3V8VX-ray2.60A/B1-702[»]
3V97X-ray2.20A/B1-702[»]
ProteinModelPortaliP75864
SMRiP75864
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260025, 148 interactors
DIPiDIP-11486N
IntActiP75864, 48 interactors
STRINGi316385.ECDH10B_1018

Proteomic databases

PaxDbiP75864
PRIDEiP75864

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74034; AAC74034; b0948
BAA35703; BAA35703; BAA35703
GeneIDi945564
KEGGiecj:JW0931
eco:b0948
PATRICifig|1411691.4.peg.1326

Organism-specific databases

EchoBASEiEB3481
EcoGeneiEG13717 rlmL

Phylogenomic databases

eggNOGiENOG4105C4H Bacteria
COG0116 LUCA
COG1092 LUCA
HOGENOMiHOG000218371
InParanoidiP75864
KOiK12297
OMAiSWDVQRD
PhylomeDBiP75864

Enzyme and pathway databases

BioCyciEcoCyc:G6488-MONOMER
MetaCyc:G6488-MONOMER
BRENDAi2.1.1.173 2026

Miscellaneous databases

PROiPR:P75864

Family and domain databases

HAMAPiMF_01858 23SrRNA_methyltr_KL, 1 hit
InterProiView protein in InterPro
IPR017244 23SrRNA_methyltr_KL
IPR002052 DNA_methylase_N6_adenine_CS
IPR000241 RNA_methylase_dom
IPR019614 SAM-dep_methyl-trfase
IPR029063 SAM-dependent_MTases
IPR004114 THUMP_dom
PfamiView protein in Pfam
PF10672 Methyltrans_SAM, 1 hit
PF02926 THUMP, 1 hit
PF01170 UPF0020, 1 hit
PIRSFiPIRSF037618 RNA_Mtase_bacteria_prd, 1 hit
SMARTiView protein in SMART
SM00981 THUMP, 1 hit
SUPFAMiSSF53335 SSF53335, 3 hits
PROSITEiView protein in PROSITE
PS51165 THUMP, 1 hit
PS01261 UPF0020, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRLMKL_ECOLI
AccessioniPrimary (citable) accession number: P75864
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: October 10, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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