UniProtKB - P75830 (MACA_ECOLI)
Protein
Macrolide export protein MacA
Gene
macA
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the tripartite efflux system MacAB-TolC. MacA stimulates the ATPase activity of MacB by promoting the closed ATP-bound state of MacB, increases the capacity of MacB to bind macrolides such as erythromycin, and provides a physical link between MacB and TolC. When overexpressed, the system confers resistance against macrolides composed of 14- and 15-membered lactones but no or weak resistance against 16-membered ones. In addition, MacA binds tightly rough-core lipopolysaccharide (R-LPS), suggesting that the system could also transport R-LPS or a similar glycolipid.5 Publications
GO - Molecular functioni
- efflux transmembrane transporter activity Source: GO_Central
- identical protein binding Source: EcoCyc
GO - Biological processi
- response to antibiotic Source: UniProtKB-KW
- xenobiotic detoxification by transmembrane export across the plasma membrane Source: InterPro
Keywordsi
Biological process | Antibiotic resistance, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:MACA MetaCyc:MACA |
Protein family/group databases
TCDBi | 3.A.1.122.1, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Macrolide export protein MacA |
Gene namesi | Name:macA Synonyms:ybjY Ordered Locus Names:b0878, JW0862 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 2 Publications; Single-pass membrane protein 2 Publications; Periplasmic side 2 Publications
Plasma Membrane
- plasma membrane Source: EcoCyc
Other locations
- efflux pump complex Source: GO_Central
- extrinsic component of membrane Source: EcoliWiki
- MacAB-TolC complex Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 10 | CytoplasmicSequence analysis | 10 | |
Transmembranei | 11 – 31 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 32 – 371 | PeriplasmicSequence analysisAdd BLAST | 340 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 353 | G → A: 2-fold increase in susceptibility to macrolides. Does not affect oligomerization and ability to interact with MacB and TolC, but changes the structure of the MP region. Does not stimulate MacB ATPase activity. 1 Publication | 1 | |
Mutagenesisi | 353 | G → C: 4-fold increase in susceptibility to macrolides. Does not stimulate MacB ATPase activity. 1 Publication | 1 | |
Mutagenesisi | 353 | G → S: 2-fold increase in susceptibility to macrolides. 1 Publication | 1 | |
Mutagenesisi | 357 | G → A: 2-fold increase in susceptibility to macrolides. Does not stimulate MacB ATPase activity. 1 Publication | 1 | |
Mutagenesisi | 357 | G → C or S: Does not affect susceptibility to macrolides. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000018695 | 1 – 371 | Macrolide export protein MacAAdd BLAST | 371 |
Proteomic databases
jPOSTi | P75830 |
PaxDbi | P75830 |
PRIDEi | P75830 |
Expressioni
Inductioni
Expressed at very low levels under standard laboratory conditions.1 Publication
Interactioni
Subunit structurei
Homohexamer. Part of the tripartite efflux system MacAB-TolC, which is composed of an inner membrane transporter, MacB, a periplasmic membrane fusion protein, MacA, and an outer membrane component, TolC. The complex forms a large protein conduit and can translocate molecules across both the inner and outer membranes. MacA interacts with MacB and TolC, and this interaction changes its conformation.
5 PublicationsBinary interactionsi
Hide detailsP75830
With | #Exp. | IntAct |
---|---|---|
itself | 4 | EBI-551961,EBI-551961 |
macB [P75831] | 3 | EBI-551961,EBI-1125580 |
tolC [P02930] | 3 | EBI-551961,EBI-875614 |
GO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4260003, 406 interactors |
ComplexPortali | CPX-2107, MacAB-TolC ABC transporter complex |
DIPi | DIP-11460N |
IntActi | P75830, 11 interactors |
STRINGi | 511145.b0878 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P75830 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P75830 |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 92 – 137 | Sequence analysisAdd BLAST | 46 |
Domaini
The periplasmic domain is sufficient to bind MacB. The membrane proximal (MP) region plays a critical role in the stimulation of MacB ATPase activity.2 Publications
Sequence similaritiesi
Keywords - Domaini
Coiled coil, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0845, Bacteria |
HOGENOMi | CLU_018816_14_1_6 |
InParanoidi | P75830 |
PhylomeDBi | P75830 |
Family and domain databases
InterProi | View protein in InterPro IPR032317, HlyD_D23 IPR030190, MacA IPR006143, RND_pump_MFP |
PANTHERi | PTHR30469:SF34, PTHR30469:SF34, 1 hit |
Pfami | View protein in Pfam PF16576, HlyD_D23, 1 hit |
TIGRFAMsi | TIGR01730, RND_mfp, 1 hit |
i Sequence
Sequence statusi: Complete.
P75830-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKRKTVKKR YVIALVIVIA GLITLWRILN APVPTYQTLI VRPGDLQQSV
60 70 80 90 100
LATGKLDALR KVDVGAQVSG QLKTLSVAIG DKVKKDQLLG VIDPEQAENQ
110 120 130 140 150
IKEVEATLME LRAQRQQAEA ELKLARVTYS RQQRLAQTKA VSQQDLDTAA
160 170 180 190 200
TEMAVKQAQI GTIDAQIKRN QASLDTAKTN LDYTRIVAPM AGEVTQITTL
210 220 230 240 250
QGQTVIAAQQ APNILTLADM SAMLVKAQVS EADVIHLKPG QKAWFTVLGD
260 270 280 290 300
PLTRYEGQIK DVLPTPEKVN DAIFYYARFE VPNPNGLLRL DMTAQVHIQL
310 320 330 340 350
TDVKNVLTIP LSALGDPVGD NRYKVKLLRN GETREREVTI GARNDTDVEI
360 370
VKGLEAGDEV VIGEAKPGAA Q
Sequence cautioni
The sequence BAB64541 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB071145 Genomic DNA Translation: BAB64541.1 Different initiation. U00096 Genomic DNA Translation: AAC73965.2 AP009048 Genomic DNA Translation: BAA35597.1 |
PIRi | F64826 |
RefSeqi | NP_415399.4, NC_000913.3 WP_000746442.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC73965; AAC73965; b0878 BAA35597; BAA35597; BAA35597 |
GeneIDi | 947322 |
KEGGi | ecj:JW0862 eco:b0878 |
PATRICi | fig|1411691.4.peg.1399 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB071145 Genomic DNA Translation: BAB64541.1 Different initiation. U00096 Genomic DNA Translation: AAC73965.2 AP009048 Genomic DNA Translation: BAA35597.1 |
PIRi | F64826 |
RefSeqi | NP_415399.4, NC_000913.3 WP_000746442.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3FPP | X-ray | 2.99 | A/B | 31-371 | [»] | |
5NIK | electron microscopy | 3.30 | D/E/F/G/H/I | 1-371 | [»] | |
5NIL | electron microscopy | 5.30 | D/E/F/G/H/I | 1-371 | [»] | |
SMRi | P75830 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260003, 406 interactors |
ComplexPortali | CPX-2107, MacAB-TolC ABC transporter complex |
DIPi | DIP-11460N |
IntActi | P75830, 11 interactors |
STRINGi | 511145.b0878 |
Protein family/group databases
TCDBi | 3.A.1.122.1, the atp-binding cassette (abc) superfamily |
Proteomic databases
jPOSTi | P75830 |
PaxDbi | P75830 |
PRIDEi | P75830 |
Genome annotation databases
EnsemblBacteriai | AAC73965; AAC73965; b0878 BAA35597; BAA35597; BAA35597 |
GeneIDi | 947322 |
KEGGi | ecj:JW0862 eco:b0878 |
PATRICi | fig|1411691.4.peg.1399 |
Organism-specific databases
EchoBASEi | EB3458 |
Phylogenomic databases
eggNOGi | COG0845, Bacteria |
HOGENOMi | CLU_018816_14_1_6 |
InParanoidi | P75830 |
PhylomeDBi | P75830 |
Enzyme and pathway databases
BioCyci | EcoCyc:MACA MetaCyc:MACA |
Miscellaneous databases
EvolutionaryTracei | P75830 |
PROi | PR:P75830 |
Family and domain databases
InterProi | View protein in InterPro IPR032317, HlyD_D23 IPR030190, MacA IPR006143, RND_pump_MFP |
PANTHERi | PTHR30469:SF34, PTHR30469:SF34, 1 hit |
Pfami | View protein in Pfam PF16576, HlyD_D23, 1 hit |
TIGRFAMsi | TIGR01730, RND_mfp, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MACA_ECOLI | |
Accessioni | P75830Primary (citable) accession number: P75830 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | May 30, 2000 | |
Last modified: | April 7, 2021 | |
This is version 168 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families