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Protein

Hydroxylamine reductase

Gene

hcp

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the reduction of hydroxylamine to form NH3 and H2O. Is also able to reduce hydroxylamine analogs such as methylhydroxylamine and hydroxyquinone. Might have a role as a scavenger of potentially toxic by-products of nitrate metabolism.UniRule annotation2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Protein has several cofactor binding sites:

<p>This subsection of the ‘Function’ section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Inhibited by oxygen. Activated by cyanide except in the prolonged presence of excess cyanide, where the enzyme is inactivated.1 Publication

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=2.5 mM for hydroxylamine (at pH 9)1 Publication
  1. Vmax=92 µmol/min/mg enzyme (at pH 7.5)1 Publication
  2. Vmax=458 µmol/min/mg enzyme (at pH 9)1 Publication

pH dependencei

Optimum pH is 9.0.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Function’ section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi3Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi6Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi18Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi25Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi249Iron-oxo-sulfur (4Fe-2O-2S); via tele nitrogenUniRule annotation1
Metal bindingi273Iron-oxo-sulfur (4Fe-2O-2S)UniRule annotation1
Metal bindingi317Iron-oxo-sulfur (4Fe-2O-2S)UniRule annotation1
Metal bindingi405Iron-oxo-sulfur (4Fe-2O-2S); via persulfide groupUniRule annotation1
Metal bindingi433Iron-oxo-sulfur (4Fe-2O-2S)UniRule annotation1
Metal bindingi458Iron-oxo-sulfur (4Fe-2O-2S)UniRule annotation1
Metal bindingi492Iron-oxo-sulfur (4Fe-2O-2S)UniRule annotation1
Metal bindingi494Iron-oxo-sulfur (4Fe-2O-2S)UniRule annotation1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

  • nitric oxide catabolic process Source: EcoCyc
  • nitrogen compound metabolic process Source: EcoCyc
  • oxidation-reduction process Source: EcoliWiki
  • response to hydrogen peroxide Source: EcoliWiki

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionOxidoreductase
Ligand2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:G6457-MONOMER
MetaCyc:G6457-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Hydroxylamine reductase1 Publication (EC:1.7.99.11 Publication)
Alternative name(s):
Hybrid-cluster proteinUniRule annotation
Short name:
HCPUniRule annotation
Prismane protein1 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:hcpUniRule annotation
Synonyms:ybjW
Ordered Locus Names:b0873, JW0857
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

Escherichia coli strain K12 genome database

More...
EcoGenei
EG13692 hcp

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001516741 – 550Hydroxylamine reductaseAdd BLAST550

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei405Cysteine persulfideUniRule annotation1

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P75825

PRoteomics IDEntifications database

More...
PRIDEi
P75825

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

By Fnr, NarL and NarP under anaerobic conditions in the presence of either nitrate or nitrite.1 Publication

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
4262100, 10 interactors

Database of interacting proteins

More...
DIPi
DIP-9870N

Protein interaction database and analysis system

More...
IntActi
P75825, 5 interactors

STRING: functional protein association networks

More...
STRINGi
316385.ECDH10B_0943

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

More...
ProteinModelPortali
P75825

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P75825

Database of comparative protein structure models

More...
ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the HCP family.UniRule annotation

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4105EJ7 Bacteria
COG1151 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000007176

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P75825

KEGG Orthology (KO)

More...
KOi
K05601

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P75825

Family and domain databases

Conserved Domains Database

More...
CDDi
cd01914 HCP, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.20.1270.20, 2 hits
3.40.50.2030, 2 hits

HAMAP database of protein families

More...
HAMAPi
MF_00069 Hydroxylam_reduct, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR004137 HCP/CODH
IPR010048 Hydroxylam_reduct
IPR016099 Prismane-like_a/b-sand
IPR011254 Prismane-like_sf
IPR016100 Prismane_a-bundle

The PANTHER Classification System

More...
PANTHERi
PTHR30109 PTHR30109, 1 hit
PTHR30109:SF0 PTHR30109:SF0, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF03063 Prismane, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF000076 HCP, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF56821 SSF56821, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR01703 hybrid_clust, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P75825-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFCVQCEQTI RTPAGNGCSY AQGMCGKTAE TSDLQDLLIA ALQGLSAWAV
60 70 80 90 100
KAREYGIINH DVDSFAPRAF FSTLTNVNFD SPRIVGYARE AIALREALKA
110 120 130 140 150
QCLAVDANAR VDNPMADLQL VSDDLGELQR QAAEFTPNKD KAAIGENILG
160 170 180 190 200
LRLLCLYGLK GAAAYMEHAH VLGQYDNDIY AQYHKIMAWL GTWPADMNAL
210 220 230 240 250
LECSMEIGQM NFKVMSILDA GETGKYGHPT PTQVNVKATA GKCILISGHD
260 270 280 290 300
LKDLYNLLEQ TEGTGVNVYT HGEMLPAHGY PELRKFKHLV GNYGSGWQNQ
310 320 330 340 350
QVEFARFPGP IVMTSNCIID PTVGAYDDRI WTRSIVGWPG VRHLDGDDFS
360 370 380 390 400
AVITQAQQMA GFPYSEIPHL ITVGFGRQTL LGAADTLIDL VSREKLRHIF
410 420 430 440 450
LLGGCDGARG ERHYFTDFAT SVPDDCLILT LACGKYRFNK LEFGDIEGLP
460 470 480 490 500
RLVDAGQCND AYSAIILAVT LAEKLGCGVN DLPLSLVLSW FEQKAIVILL
510 520 530 540 550
TLLSLGVKNI VTGPTAPGFL TPDLLAVLNE KFGLRSITTV EEDMKQLLSA
Length:550
Mass (Da):60,064
Last modified:May 30, 2000 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iCA4958B3143B1F48
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti134E → V in AAC73960 (PubMed:8905232).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC73960.2
AP009048 Genomic DNA Translation: BAA35587.2

Protein sequence database of the Protein Information Resource

More...
PIRi
A64826

NCBI Reference Sequences

More...
RefSeqi
NP_415394.4, NC_000913.3
WP_000458809.1, NZ_LN832404.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC73960; AAC73960; b0873
BAA35587; BAA35587; BAA35587

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
946592

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW0857
eco:b0873

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.1404

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC73960.2
AP009048 Genomic DNA Translation: BAA35587.2
PIRiA64826
RefSeqiNP_415394.4, NC_000913.3
WP_000458809.1, NZ_LN832404.1

3D structure databases

ProteinModelPortaliP75825
SMRiP75825
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262100, 10 interactors
DIPiDIP-9870N
IntActiP75825, 5 interactors
STRINGi316385.ECDH10B_0943

Proteomic databases

PaxDbiP75825
PRIDEiP75825

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73960; AAC73960; b0873
BAA35587; BAA35587; BAA35587
GeneIDi946592
KEGGiecj:JW0857
eco:b0873
PATRICifig|1411691.4.peg.1404

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB3456
EcoGeneiEG13692 hcp

Phylogenomic databases

eggNOGiENOG4105EJ7 Bacteria
COG1151 LUCA
HOGENOMiHOG000007176
InParanoidiP75825
KOiK05601
PhylomeDBiP75825

Enzyme and pathway databases

BioCyciEcoCyc:G6457-MONOMER
MetaCyc:G6457-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P75825

Family and domain databases

CDDicd01914 HCP, 1 hit
Gene3Di1.20.1270.20, 2 hits
3.40.50.2030, 2 hits
HAMAPiMF_00069 Hydroxylam_reduct, 1 hit
InterProiView protein in InterPro
IPR004137 HCP/CODH
IPR010048 Hydroxylam_reduct
IPR016099 Prismane-like_a/b-sand
IPR011254 Prismane-like_sf
IPR016100 Prismane_a-bundle
PANTHERiPTHR30109 PTHR30109, 1 hit
PTHR30109:SF0 PTHR30109:SF0, 1 hit
PfamiView protein in Pfam
PF03063 Prismane, 1 hit
PIRSFiPIRSF000076 HCP, 1 hit
SUPFAMiSSF56821 SSF56821, 1 hit
TIGRFAMsiTIGR01703 hybrid_clust, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiHCP_ECOLI
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P75825
Secondary accession number(s): Q9R7R0, Q9R7R1
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 30, 2000
Last modified: December 5, 2018
This is version 149 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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