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Protein

N-acetylmuramoyl-L-alanine amidase AmiD

Gene

amiD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

Cofactori

Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi50Zinc; catalytic1 Publication1
Active sitei119Proton acceptor1 Publication1
Metal bindingi166Zinc; catalytic1 Publication1
Sitei174Transition state stabilizer1
Metal bindingi176Zinc; catalytic1 Publication1

GO - Molecular functioni

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • peptidoglycan catabolic process Source: EcoCyc
  • peptidoglycan turnover Source: GO_Central

Keywordsi

Molecular functionHydrolase
Biological processCell wall biogenesis/degradation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:G6452-MONOMER
MetaCyc:G6452-MONOMER
BRENDAi3.5.1.28 2026

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylmuramoyl-L-alanine amidase AmiD (EC:3.5.1.28)
Gene namesi
Name:amiD
Synonyms:ybjR
Ordered Locus Names:b0867, JW0851
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13687 amiD

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16PROSITE-ProRule annotationAdd BLAST16
ChainiPRO_000016441717 – 276N-acetylmuramoyl-L-alanine amidase AmiDAdd BLAST260

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi17N-palmitoyl cysteinePROSITE-ProRule annotation1
Lipidationi17S-diacylglycerol cysteinePROSITE-ProRule annotation1

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiP75820
PRIDEiP75820

Interactioni

Protein-protein interaction databases

BioGridi4259998, 12 interactors
STRINGi316385.ECDH10B_0937

Structurei

Secondary structure

1276
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP75820
SMRiP75820
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP75820

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 179N-acetylmuramoyl-L-alanine amidaseSequence analysisAdd BLAST138

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni51 – 52Substrate binding2

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108VBV Bacteria
COG3023 LUCA
HOGENOMiHOG000255964
InParanoidiP75820
KOiK11066
PhylomeDBiP75820

Family and domain databases

CDDicd06583 PGRP, 1 hit
Gene3Di1.10.101.10, 1 hit
3.40.80.10, 1 hit
InterProiView protein in InterPro
IPR036505 Amidase/PGRP_sf
IPR002502 Amidase_domain
IPR036365 PGBD-like_sf
IPR036366 PGBDSf
PfamiView protein in Pfam
PF01510 Amidase_2, 1 hit
SMARTiView protein in SMART
SM00644 Ami_2, 1 hit
SUPFAMiSSF47090 SSF47090, 1 hit
SSF55846 SSF55846, 1 hit
PROSITEiView protein in PROSITE
PS51257 PROKAR_LIPOPROTEIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P75820-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRRFFWLVAA ALLLAGCAGE KGIVEKEGYQ LDTRRQAQAA YPRIKVLVIH
60 70 80 90 100
YTADDFDSSL ATLTDKQVSS HYLVPAVPPR YNGKPRIWQL VPEQELAWHA
110 120 130 140 150
GISAWRGATR LNDTSIGIEL ENRGWQKSAG VKYFAPFEPA QIQALIPLAK
160 170 180 190 200
DIIARYHIKP ENVVAHADIA PQRKDDPGPL FPWQQLAQQG IGAWPDAQRV
210 220 230 240 250
NFYLAGRAPH TPVDTASLLE LLARYGYDVK PDMTPREQRR VIMAFQMHFR
260 270
PTLYNGEADA ETQAIAEALL EKYGQD
Length:276
Mass (Da):31,072
Last modified:February 1, 1997 - v1
Checksum:iE6E4F642AFF548CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC73954.1
AP009048 Genomic DNA Translation: BAA35581.1
PIRiC64825
RefSeqiNP_415388.1, NC_000913.3
WP_001252135.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73954; AAC73954; b0867
BAA35581; BAA35581; BAA35581
GeneIDi945494
KEGGiecj:JW0851
eco:b0867
PATRICifig|1411691.4.peg.1410

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC73954.1
AP009048 Genomic DNA Translation: BAA35581.1
PIRiC64825
RefSeqiNP_415388.1, NC_000913.3
WP_001252135.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BH7X-ray2.20A18-276[»]
2WKXX-ray1.80A18-276[»]
3D2YX-ray1.75A18-276[»]
3D2ZX-ray2.80A18-276[»]
ProteinModelPortaliP75820
SMRiP75820
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259998, 12 interactors
STRINGi316385.ECDH10B_0937

Proteomic databases

PaxDbiP75820
PRIDEiP75820

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73954; AAC73954; b0867
BAA35581; BAA35581; BAA35581
GeneIDi945494
KEGGiecj:JW0851
eco:b0867
PATRICifig|1411691.4.peg.1410

Organism-specific databases

EchoBASEiEB3451
EcoGeneiEG13687 amiD

Phylogenomic databases

eggNOGiENOG4108VBV Bacteria
COG3023 LUCA
HOGENOMiHOG000255964
InParanoidiP75820
KOiK11066
PhylomeDBiP75820

Enzyme and pathway databases

BioCyciEcoCyc:G6452-MONOMER
MetaCyc:G6452-MONOMER
BRENDAi3.5.1.28 2026

Miscellaneous databases

EvolutionaryTraceiP75820
PROiPR:P75820

Family and domain databases

CDDicd06583 PGRP, 1 hit
Gene3Di1.10.101.10, 1 hit
3.40.80.10, 1 hit
InterProiView protein in InterPro
IPR036505 Amidase/PGRP_sf
IPR002502 Amidase_domain
IPR036365 PGBD-like_sf
IPR036366 PGBDSf
PfamiView protein in Pfam
PF01510 Amidase_2, 1 hit
SMARTiView protein in SMART
SM00644 Ami_2, 1 hit
SUPFAMiSSF47090 SSF47090, 1 hit
SSF55846 SSF55846, 1 hit
PROSITEiView protein in PROSITE
PS51257 PROKAR_LIPOPROTEIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAMID_ECOLI
AccessioniPrimary (citable) accession number: P75820
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: February 1, 1997
Last modified: November 7, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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