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UniProtKB - P75820 (AMID_ECOLI)
Protein
N-acetylmuramoyl-L-alanine amidase AmiD
Gene
amiD
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalytic activityi
- Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. EC:3.5.1.28
Cofactori
Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 50 | Zinc; catalytic1 Publication | 1 | |
Active sitei | 119 | Proton acceptor1 Publication | 1 | |
Metal bindingi | 166 | Zinc; catalytic1 Publication | 1 | |
Sitei | 174 | Transition state stabilizer1 Publication | 1 | |
Metal bindingi | 176 | Zinc; catalytic1 Publication | 1 |
GO - Molecular functioni
- N-acetyl-anhydromuramoyl-L-alanine amidase activity Source: EcoCyc
- N-acetylmuramoyl-L-alanine amidase activity Source: EcoCyc
- zinc ion binding Source: EcoliWiki
GO - Biological processi
- cell wall organization Source: UniProtKB-KW
- peptidoglycan catabolic process Source: EcoCyc
- peptidoglycan turnover Source: GO_Central
Keywordsi
Molecular function | Hydrolase |
Biological process | Cell wall biogenesis/degradation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:G6452-MONOMER |
BRENDAi | 3.5.1.28, 2026 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:amiD Synonyms:ybjR Ordered Locus Names:b0867, JW0851 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Other locations
- Cell outer membrane Curated; Lipid-anchor Curated
Other locations
- cell outer membrane Source: UniProtKB-SubCell
- outer membrane Source: EcoliWiki
Keywords - Cellular componenti
Cell outer membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 16 | PROSITE-ProRule annotationAdd BLAST | 16 | |
ChainiPRO_0000164417 | 17 – 276 | N-acetylmuramoyl-L-alanine amidase AmiDAdd BLAST | 260 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 17 | N-palmitoyl cysteinePROSITE-ProRule annotation | 1 | |
Lipidationi | 17 | S-diacylglycerol cysteinePROSITE-ProRule annotation | 1 |
Keywords - PTMi
Lipoprotein, PalmitateProteomic databases
jPOSTi | P75820 |
PaxDbi | P75820 |
PRIDEi | P75820 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259998, 12 interactors |
STRINGi | 511145.b0867 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P75820 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P75820 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 42 – 179 | N-acetylmuramoyl-L-alanine amidaseSequence analysisAdd BLAST | 138 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 51 – 52 | Substrate binding | 2 |
Sequence similaritiesi
Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG3023, Bacteria |
HOGENOMi | CLU_049290_2_1_6 |
InParanoidi | P75820 |
PhylomeDBi | P75820 |
Family and domain databases
CDDi | cd06583, PGRP, 1 hit |
Gene3Di | 1.10.101.10, 1 hit 3.40.80.10, 1 hit |
InterProi | View protein in InterPro IPR036505, Amidase/PGRP_sf IPR002502, Amidase_domain IPR036365, PGBD-like_sf IPR036366, PGBDSf |
Pfami | View protein in Pfam PF01510, Amidase_2, 1 hit |
SMARTi | View protein in SMART SM00644, Ami_2, 1 hit |
SUPFAMi | SSF47090, SSF47090, 1 hit SSF55846, SSF55846, 1 hit |
PROSITEi | View protein in PROSITE PS51257, PROKAR_LIPOPROTEIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P75820-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRRFFWLVAA ALLLAGCAGE KGIVEKEGYQ LDTRRQAQAA YPRIKVLVIH
60 70 80 90 100
YTADDFDSSL ATLTDKQVSS HYLVPAVPPR YNGKPRIWQL VPEQELAWHA
110 120 130 140 150
GISAWRGATR LNDTSIGIEL ENRGWQKSAG VKYFAPFEPA QIQALIPLAK
160 170 180 190 200
DIIARYHIKP ENVVAHADIA PQRKDDPGPL FPWQQLAQQG IGAWPDAQRV
210 220 230 240 250
NFYLAGRAPH TPVDTASLLE LLARYGYDVK PDMTPREQRR VIMAFQMHFR
260 270
PTLYNGEADA ETQAIAEALL EKYGQD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC73954.1 AP009048 Genomic DNA Translation: BAA35581.1 |
PIRi | C64825 |
RefSeqi | NP_415388.1, NC_000913.3 WP_001252135.1, NZ_SSZK01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAC73954; AAC73954; b0867 BAA35581; BAA35581; BAA35581 |
GeneIDi | 945494 |
KEGGi | ecj:JW0851 eco:b0867 |
PATRICi | fig|1411691.4.peg.1410 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC73954.1 AP009048 Genomic DNA Translation: BAA35581.1 |
PIRi | C64825 |
RefSeqi | NP_415388.1, NC_000913.3 WP_001252135.1, NZ_SSZK01000002.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2BH7 | X-ray | 2.20 | A | 18-276 | [»] | |
2WKX | X-ray | 1.80 | A | 18-276 | [»] | |
3D2Y | X-ray | 1.75 | A | 18-276 | [»] | |
3D2Z | X-ray | 2.80 | A | 18-276 | [»] | |
SMRi | P75820 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259998, 12 interactors |
STRINGi | 511145.b0867 |
Proteomic databases
jPOSTi | P75820 |
PaxDbi | P75820 |
PRIDEi | P75820 |
Genome annotation databases
EnsemblBacteriai | AAC73954; AAC73954; b0867 BAA35581; BAA35581; BAA35581 |
GeneIDi | 945494 |
KEGGi | ecj:JW0851 eco:b0867 |
PATRICi | fig|1411691.4.peg.1410 |
Organism-specific databases
EchoBASEi | EB3451 |
Phylogenomic databases
eggNOGi | COG3023, Bacteria |
HOGENOMi | CLU_049290_2_1_6 |
InParanoidi | P75820 |
PhylomeDBi | P75820 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6452-MONOMER |
BRENDAi | 3.5.1.28, 2026 |
Miscellaneous databases
EvolutionaryTracei | P75820 |
PROi | PR:P75820 |
Family and domain databases
CDDi | cd06583, PGRP, 1 hit |
Gene3Di | 1.10.101.10, 1 hit 3.40.80.10, 1 hit |
InterProi | View protein in InterPro IPR036505, Amidase/PGRP_sf IPR002502, Amidase_domain IPR036365, PGBD-like_sf IPR036366, PGBDSf |
Pfami | View protein in Pfam PF01510, Amidase_2, 1 hit |
SMARTi | View protein in SMART SM00644, Ami_2, 1 hit |
SUPFAMi | SSF47090, SSF47090, 1 hit SSF55846, SSF55846, 1 hit |
PROSITEi | View protein in PROSITE PS51257, PROKAR_LIPOPROTEIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AMID_ECOLI | |
Accessioni | P75820Primary (citable) accession number: P75820 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | February 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 154 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families