UniProtKB - P75800 (PDEI_ECOLI)
Protein
Probable cyclic di-GMP phosphodiesterase PdeI
Gene
pdeI
Organism
Escherichia coli (strain K12)
Status
Functioni
Phosphodiesterase (PDE) that catalyzes the hydrolysis of cyclic-di-GMP (c-di-GMP) to 5'-pGpG (By similarity). Overexpression reduces biofilm formation. Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria (PubMed:19460094).By similarity1 Publication
Catalytic activityi
- EC:3.1.4.52By similarity
GO - Molecular functioni
- cyclic-guanylate-specific phosphodiesterase activity Source: EcoCyc
Keywordsi
Molecular function | Hydrolase |
Ligand | c-di-GMP |
Enzyme and pathway databases
BioCyci | EcoCyc:G6433-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:pdeI1 Publication Synonyms:yliE Ordered Locus Names:b0833, JW0817 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell membrane Curated; Multi-pass membrane protein Sequence analysis
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 12 – 32 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 286 – 306 | HelicalSequence analysisAdd BLAST | 21 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Cells lacking this gene have a weak reduction in swarm size.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000168729 | 1 – 782 | Probable cyclic di-GMP phosphodiesterase PdeIAdd BLAST | 782 |
Proteomic databases
jPOSTi | P75800 |
PaxDbi | P75800 |
PRIDEi | P75800 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259986, 11 interactors |
STRINGi | 511145.b0833 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 527 – 781 | EALPROSITE-ProRule annotationAdd BLAST | 255 |
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2200, Bacteria |
HOGENOMi | CLU_000445_78_2_6 |
PhylomeDBi | P75800 |
Family and domain databases
CDDi | cd01948, EAL, 1 hit |
Gene3Di | 3.20.20.450, 1 hit |
InterProi | View protein in InterPro IPR033418, CHASE9 IPR001633, EAL_dom IPR035919, EAL_sf |
Pfami | View protein in Pfam PF17153, CHASE9, 1 hit PF00563, EAL, 1 hit |
SMARTi | View protein in SMART SM00052, EAL, 1 hit |
SUPFAMi | SSF141868, SSF141868, 1 hit |
PROSITEi | View protein in PROSITE PS50883, EAL, 1 hit |
i Sequence
Sequence statusi: Complete.
P75800-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLSLYEKIKI RLIILFLLAA LSFIGLFFII NYQLVSERAV KRADSRFELI
60 70 80 90 100
QKNVGYFFKD IERSALTLKD SLYLLKNTEE IQRAVILKME MMPFLDSVGL
110 120 130 140 150
VLDDNKYYLF SRRANDKIVV YHQEQVNGPL VDESGRVIFA DFNPSKRPWS
160 170 180 190 200
VASDDSNNSW NPAYNCFDRP GKKCISFTLH INGKDHDLLA VDKIHVDLNW
210 220 230 240 250
RYLNEYLDQI SANDEVLFLK QGHEIIAKNQ LAREKLIIYN SEGNYNIIDS
260 270 280 290 300
VDTEYIEKTS AVPNNALFEI YFYYPGGNLL NASDKLFYLP FAFIIIVLLV
310 320 330 340 350
VYLMTTRVFR RQFSEMTELV NTLAFLPDST DQIEALKIRE GDAKEIISIK
360 370 380 390 400
NSIAEMKDAE IERSNKLLSL ISYDQESGFI KNMAIIESNN NQYLAVGIIK
410 420 430 440 450
LCGLEAVEAV FGVDERNKIV RKLCQRIAEK YAQCCDIVTF NADLYLLLCR
460 470 480 490 500
ENVQTFTRKI AMVNDFDSSF GYRNLRIHKS AICEPLQGEN AWSYAEKLKL
510 520 530 540 550
AISSIRDHMF SEFIFCDDAK LNEIEENIWI ARNIRHAMEI GELFLVYQPI
560 570 580 590 600
VDINTRAILG AEALCRWVSA ERGIISPLKF ITIAEDIGFI NELGYQIIKT
610 620 630 640 650
AMGEFRHFSQ RASLKDDFLL HINVSPWQLN EPHFHERFTT IMKENGLKAN
660 670 680 690 700
SLCVEITETV IERINEHFYL NIEQLRKQGV RISIDDFGTG LSNLKRFYEI
710 720 730 740 750
NPDSIKVDSQ FTGDIFGTAG KIVRIIFDLA RYNRIPVIAE GVESEDVARE
760 770 780
LIKLGCVQAQ GYLYQKPMPF SAWDKSGKLV KE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC73920.1 AP009048 Genomic DNA Translation: BAA35528.1 |
PIRi | A64821 |
RefSeqi | NP_415354.1, NC_000913.3 WP_001360263.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC73920; AAC73920; b0833 BAA35528; BAA35528; BAA35528 |
GeneIDi | 945462 |
KEGGi | ecj:JW0817 eco:b0833 |
PATRICi | fig|1411691.4.peg.1445 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC73920.1 AP009048 Genomic DNA Translation: BAA35528.1 |
PIRi | A64821 |
RefSeqi | NP_415354.1, NC_000913.3 WP_001360263.1, NZ_LN832404.1 |
3D structure databases
SMRi | P75800 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259986, 11 interactors |
STRINGi | 511145.b0833 |
Proteomic databases
jPOSTi | P75800 |
PaxDbi | P75800 |
PRIDEi | P75800 |
Genome annotation databases
EnsemblBacteriai | AAC73920; AAC73920; b0833 BAA35528; BAA35528; BAA35528 |
GeneIDi | 945462 |
KEGGi | ecj:JW0817 eco:b0833 |
PATRICi | fig|1411691.4.peg.1445 |
Organism-specific databases
EchoBASEi | EB3249 |
Phylogenomic databases
eggNOGi | COG2200, Bacteria |
HOGENOMi | CLU_000445_78_2_6 |
PhylomeDBi | P75800 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6433-MONOMER |
Miscellaneous databases
PROi | PR:P75800 |
Family and domain databases
CDDi | cd01948, EAL, 1 hit |
Gene3Di | 3.20.20.450, 1 hit |
InterProi | View protein in InterPro IPR033418, CHASE9 IPR001633, EAL_dom IPR035919, EAL_sf |
Pfami | View protein in Pfam PF17153, CHASE9, 1 hit PF00563, EAL, 1 hit |
SMARTi | View protein in SMART SM00052, EAL, 1 hit |
SUPFAMi | SSF141868, SSF141868, 1 hit |
PROSITEi | View protein in PROSITE PS50883, EAL, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PDEI_ECOLI | |
Accessioni | P75800Primary (citable) accession number: P75800 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | February 1, 1997 | |
Last modified: | October 7, 2020 | |
This is version 124 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |