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Protein

Aldehyde reductase YahK

Gene

yahK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the reduction of a wide range of aldehydes into their corresponding alcohols. Has a strong preference for NADPH over NADH as the electron donor. Cannot use a ketone as substrate. Is a major source of NADPH-dependent aldehyde reductase activity in E.coli. The in vivo functions of YahK has yet to be determined.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Zn2+2 PublicationsNote: Binds 2 Zn2+ ions per subunit.2 Publications

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 11.2 sec(-1) for acetaldehyde reduction. kcat is 11.6 sec(-1) for propionaldehyde reduction. kcat is 12.3 sec(-1) for glyceraldehyde reduction. kcat is 41.6 sec(-1) for butyraldehyde reduction. kcat is 32.1 sec(-1) for isobutyraldehyde reduction. kcat is 32.6 sec(-1) for crotonaldehyde reduction. kcat is 13.4 sec(-1) for glutaraldehyde reduction. kcat is 0.18 sec(-1) for 5-hydroxyvalerate reduction. kcat is 18.3 sec(-1) for hexanaldehyde reduction. kcat is 7.75 sec(-1) for benzaldehyde reduction. kcat is 12.5 sec(-1) for furfural reduction. kcat is 4.7 sec(-1) for butanol oxidation. kcat is 6.7 sec(-1) for 1,4-butanediol oxidation.
  1. KM=13.3 mM for acetaldehyde1 Publication
  2. KM=10.9 mM for propionaldehyde1 Publication
  3. KM=4.4 mM for glyceraldehyde1 Publication
  4. KM=2.1 mM for butyraldehyde1 Publication
  5. KM=2.2 mM for isobutyraldehyde1 Publication
  6. KM=3.6 mM for crotonaldehyde1 Publication
  7. KM=4.1 mM for glutaraldehyde1 Publication
  8. KM=52.6 mM for 5-hydroxyvalerate1 Publication
  9. KM=0.37 mM for hexanaldehyde1 Publication
  10. KM=0.29 mM for benzaldehyde1 Publication
  11. KM=0.135 mM for furfural1 Publication
  12. KM=6.6 mM for butanol1 Publication
  13. KM=38.5 mM for 1,4-butanediol1 Publication
  14. KM=0.011 mM for NADPH1 Publication
  15. KM=0.012 mM for NADP+1 Publication

    Temperature dependencei

    Shows a constant increase in activity until 60 degrees Celsius using butyraldehyde as substrate.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Function’ section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi40Zinc 1; catalytic1
    Metal bindingi62Zinc 1; catalytic1
    Metal bindingi93Zinc 21
    Metal bindingi96Zinc 21
    Metal bindingi99Zinc 21
    Metal bindingi107Zinc 21
    Metal bindingi158Zinc 1; catalytic1

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    • alcohol dehydrogenase (NADP+) activity Source: EcoCyc
    • zinc ion binding Source: InterPro

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionOxidoreductase
    LigandMetal-binding, NADP, Zinc

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    EcoCyc:G6190-MONOMER
    MetaCyc:G6190-MONOMER

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Aldehyde reductase YahK (EC:1.1.1.2)
    Alternative name(s):
    Zinc-dependent alcohol dehydrogenase YahK
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:yahK
    Ordered Locus Names:b0325, JW0317
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    Escherichia coli strain K12 genome database

    More...
    EcoGenei
    EG13595 yahK

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001608881 – 349Aldehyde reductase YahKAdd BLAST349

    Proteomic databases

    Encyclopedia of Proteome Dynamics

    More...
    EPDi
    P75691

    jPOST - Japan Proteome Standard Repository/Database

    More...
    jPOSTi
    P75691

    PaxDb, a database of protein abundance averages across all three domains of life

    More...
    PaxDbi
    P75691

    PRoteomics IDEntifications database

    More...
    PRIDEi
    P75691

    2D gel databases

    Two-dimensional polyacrylamide gel electrophoresis database from the Geneva University Hospital

    More...
    SWISS-2DPAGEi
    P75691

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    Protein-protein interaction databases

    The Biological General Repository for Interaction Datasets (BioGrid)

    More...
    BioGridi
    4259802, 7 interactors

    Database of interacting proteins

    More...
    DIPi
    DIP-11263N

    Protein interaction database and analysis system

    More...
    IntActi
    P75691, 10 interactors

    STRING: functional protein association networks

    More...
    STRINGi
    316407.85674468

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    Secondary structure

    1349
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    Select the link destinations:

    Protein Data Bank Europe

    More...
    PDBei

    Protein Data Bank RCSB

    More...
    RCSB PDBi

    Protein Data Bank Japan

    More...
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1UUFX-ray1.76A2-349[»]

    Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

    More...
    ProteinModelPortali
    P75691

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    P75691

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    Miscellaneous databases

    Relative evolutionary importance of amino acids within a protein sequence

    More...
    EvolutionaryTracei
    P75691

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    <p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    ENOG4105DQ4 Bacteria
    COG1064 LUCA

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    HOG000294667

    InParanoid: Eukaryotic Ortholog Groups

    More...
    InParanoidi
    P75691

    KEGG Orthology (KO)

    More...
    KOi
    K13979

    Database for complete collections of gene phylogenies

    More...
    PhylomeDBi
    P75691

    Family and domain databases

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR013149 ADH_C
    IPR013154 ADH_N
    IPR002328 ADH_Zn_CS
    IPR029752 D-isomer_DH_CS1
    IPR011032 GroES-like_sf
    IPR036291 NAD(P)-bd_dom_sf
    IPR020843 PKS_ER

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF08240 ADH_N, 1 hit
    PF00107 ADH_zinc_N, 1 hit

    Simple Modular Architecture Research Tool; a protein domain database

    More...
    SMARTi
    View protein in SMART
    SM00829 PKS_ER, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF50129 SSF50129, 1 hit
    SSF51735 SSF51735, 1 hit

    PROSITE; a protein domain and family database

    More...
    PROSITEi
    View protein in PROSITE
    PS00059 ADH_ZINC, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    P75691-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MKIKAVGAYS AKQPLEPMDI TRREPGPNDV KIEIAYCGVC HSDLHQVRSE
    60 70 80 90 100
    WAGTVYPCVP GHEIVGRVVA VGDQVEKYAP GDLVGVGCIV DSCKHCEECE
    110 120 130 140 150
    DGLENYCDHM TGTYNSPTPD EPGHTLGGYS QQIVVHERYV LRIRHPQEQL
    160 170 180 190 200
    AAVAPLLCAG ITTYSPLRHW QAGPGKKVGV VGIGGLGHMG IKLAHAMGAH
    210 220 230 240 250
    VVAFTTSEAK REAAKALGAD EVVNSRNADE MAAHLKSFDF ILNTVAAPHN
    260 270 280 290 300
    LDDFTTLLKR DGTMTLVGAP ATPHKSPEVF NLIMKRRAIA GSMIGGIPET
    310 320 330 340
    QEMLDFCAEH GIVADIEMIR ADQINEAYER MLRGDVKYRF VIDNRTLTD
    Length:349
    Mass (Da):37,978
    Last modified:February 1, 1997 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iAD0E2DF4D43C9B09
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti116S → L in AAB18051 (Ref. 1) Curated1
    Sequence conflicti145H → Y in AAB18051 (Ref. 1) Curated1

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    U73857 Genomic DNA Translation: AAB18051.1
    U00096 Genomic DNA Translation: AAC73428.1
    AP009048 Genomic DNA Translation: BAE76108.1

    Protein sequence database of the Protein Information Resource

    More...
    PIRi
    E64759

    NCBI Reference Sequences

    More...
    RefSeqi
    NP_414859.1, NC_000913.3
    WP_000692754.1, NZ_LN832404.1

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...
    EnsemblBacteriai
    AAC73428; AAC73428; b0325
    BAE76108; BAE76108; BAE76108

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    944975

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    ecj:JW0317
    eco:b0325

    Pathosystems Resource Integration Center (PATRIC)

    More...
    PATRICi
    fig|1411691.4.peg.1952

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U73857 Genomic DNA Translation: AAB18051.1
    U00096 Genomic DNA Translation: AAC73428.1
    AP009048 Genomic DNA Translation: BAE76108.1
    PIRiE64759
    RefSeqiNP_414859.1, NC_000913.3
    WP_000692754.1, NZ_LN832404.1

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1UUFX-ray1.76A2-349[»]
    ProteinModelPortaliP75691
    SMRiP75691
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4259802, 7 interactors
    DIPiDIP-11263N
    IntActiP75691, 10 interactors
    STRINGi316407.85674468

    2D gel databases

    SWISS-2DPAGEiP75691

    Proteomic databases

    EPDiP75691
    jPOSTiP75691
    PaxDbiP75691
    PRIDEiP75691

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC73428; AAC73428; b0325
    BAE76108; BAE76108; BAE76108
    GeneIDi944975
    KEGGiecj:JW0317
    eco:b0325
    PATRICifig|1411691.4.peg.1952

    Organism-specific databases

    EchoBASE - an integrated post-genomic database for E. coli

    More...
    EchoBASEi
    EB3364
    EcoGeneiEG13595 yahK

    Phylogenomic databases

    eggNOGiENOG4105DQ4 Bacteria
    COG1064 LUCA
    HOGENOMiHOG000294667
    InParanoidiP75691
    KOiK13979
    PhylomeDBiP75691

    Enzyme and pathway databases

    BioCyciEcoCyc:G6190-MONOMER
    MetaCyc:G6190-MONOMER

    Miscellaneous databases

    EvolutionaryTraceiP75691

    Protein Ontology

    More...
    PROi
    PR:P75691

    Family and domain databases

    InterProiView protein in InterPro
    IPR013149 ADH_C
    IPR013154 ADH_N
    IPR002328 ADH_Zn_CS
    IPR029752 D-isomer_DH_CS1
    IPR011032 GroES-like_sf
    IPR036291 NAD(P)-bd_dom_sf
    IPR020843 PKS_ER
    PfamiView protein in Pfam
    PF08240 ADH_N, 1 hit
    PF00107 ADH_zinc_N, 1 hit
    SMARTiView protein in SMART
    SM00829 PKS_ER, 1 hit
    SUPFAMiSSF50129 SSF50129, 1 hit
    SSF51735 SSF51735, 1 hit
    PROSITEiView protein in PROSITE
    PS00059 ADH_ZINC, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiYAHK_ECOLI
    <p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P75691
    Secondary accession number(s): P71306, Q2MC98
    <p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: January 16, 2019
    This is version 149 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
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