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UniProtKB - P75189 (ENO_MYCPN)
Protein
Enolase
Gene
eno
Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Functioni
Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.
UniRule annotationCatalytic activityi
- EC:4.2.1.11UniRule annotation
Cofactori
Mg2+UniRule annotation
Activity regulationi
The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation
: glycolysis Pathwayi
This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 169 | SubstrateUniRule annotation | 1 | |
Binding sitei | 178 | SubstrateUniRule annotation | 1 | |
Active sitei | 219 | Proton donorUniRule annotation | 1 | |
Metal bindingi | 256 | MagnesiumUniRule annotation | 1 | |
Metal bindingi | 310 | MagnesiumUniRule annotation | 1 | |
Binding sitei | 310 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 337 | MagnesiumUniRule annotation | 1 | |
Binding sitei | 337 | SubstrateUniRule annotation | 1 | |
Active sitei | 362 | Proton acceptorUniRule annotation | 1 | |
Binding sitei | 362 | Substrate; covalent; in inhibited formUniRule annotation | 1 | |
Binding sitei | 413 | SubstrateUniRule annotation | 1 |
GO - Molecular functioni
- magnesium ion binding Source: UniProtKB-UniRule
- phosphopyruvate hydratase activity Source: UniProtKB-UniRule
GO - Biological processi
- glycolytic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Lyase |
Biological process | Glycolysis |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00109;UER00187 |
Names & Taxonomyi
Protein namesi | Recommended name: EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation)Alternative name(s): 2-phospho-D-glycerate hydro-lyaseUniRule annotation 2-phosphoglycerate dehydrataseUniRule annotation |
Gene namesi | Name:enoUniRule annotation Ordered Locus Names:MPN_606 ORF Names:MP236 |
Organismi | Mycoplasma pneumoniae (strain ATCC 29342 / M129) |
Taxonomic identifieri | 272634 [NCBI] |
Taxonomic lineagei | Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Extracellular region or secreted
- Secreted UniRule annotation
Other locations
- Cell surface UniRule annotation
Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation
Cytosol
- cytosol Source: AgBase
- phosphopyruvate hydratase complex Source: InterPro
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Other locations
- cell surface Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000133930 | 1 – 456 | EnolaseAdd BLAST | 456 |
Interactioni
Protein-protein interaction databases
IntActi | P75189, 6 interactors |
STRINGi | 272634.MPN_606 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 389 – 392 | Substrate bindingUniRule annotation | 4 |
Sequence similaritiesi
Belongs to the enolase family.UniRule annotation
Phylogenomic databases
HOGENOMi | CLU_031223_2_1_14 |
OMAi | EFMIIPV |
Family and domain databases
CDDi | cd03313, enolase, 1 hit |
Gene3Di | 3.20.20.120, 1 hit 3.30.390.10, 1 hit |
HAMAPi | MF_00318, Enolase, 1 hit |
InterProi | View protein in InterPro IPR000941, Enolase IPR036849, Enolase-like_C_sf IPR029017, Enolase-like_N IPR020810, Enolase_C IPR020809, Enolase_CS IPR020811, Enolase_N |
PANTHERi | PTHR11902, PTHR11902, 1 hit |
Pfami | View protein in Pfam PF00113, Enolase_C, 1 hit PF03952, Enolase_N, 1 hit |
PIRSFi | PIRSF001400, Enolase, 1 hit |
PRINTSi | PR00148, ENOLASE |
SFLDi | SFLDF00002, enolase, 1 hit |
SMARTi | View protein in SMART SM01192, Enolase_C, 1 hit SM01193, Enolase_N, 1 hit |
SUPFAMi | SSF51604, SSF51604, 1 hit SSF54826, SSF54826, 1 hit |
TIGRFAMsi | TIGR01060, eno, 1 hit |
PROSITEi | View protein in PROSITE PS00164, ENOLASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P75189-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSAQTGTDLF KIADLFAYQV FDSRGFPTVA CVVKLASGHT GEAMVPSGAS
60 70 80 90 100
TGEKEAIELR DGDPKAYFGK GVSQAVQNVN QTIAPKLIGL NATDQAAIDA
110 120 130 140 150
LMIQLDGTPN KAKLGANAIL AVSLAVAKAA ASAQKTSLFK YLANQVMGLN
160 170 180 190 200
KTEFILTVPM LNVINGGAHA DNNIDFQEFM IMPLGANSMH QALKMASETF
210 220 230 240 250
HALQKLLKQR GLNTNKGDEG GFAPNLKLAE EALDLMVEAI KAAGYQPGSD
260 270 280 290 300
IAIALDVAAS EFYDDTTKRY VFKKGIKAKI LDEKEWSLTT AQMIAYLKKL
310 320 330 340 350
TEQYPIISIE DGLSEHDWEG METLTKTLGQ HIQIVGDDLY CTNPAIAEKG
360 370 380 390 400
VAHKATNSIL IKLNQIGTLT ETIKAINIAK DANWSQVISH RSGETEDTTI
410 420 430 440 450
ADLAVAACTG QIKTGSMSRS ERIAKYNRLL QIELELGNNA KYLGWNTFKN
IKPQKA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 309 – 311 | IED → HRS (PubMed:8604303).Curated | 3 | |
Sequence conflicti | 327 – 332 | TLGQHI → HWSTH in AAC43651 (PubMed:8604303).Curated | 6 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00089 Genomic DNA Translation: AAB95884.1 U43738 Genomic DNA Translation: AAC43651.1 |
PIRi | S73562 |
RefSeqi | NP_110295.1, NC_000912.1 WP_010874963.1, NC_000912.1 |
Genome annotation databases
EnsemblBacteriai | AAB95884; AAB95884; MPN_606 |
GeneIDi | 66608709 |
KEGGi | mpn:MPN_606 |
PATRICi | fig|272634.6.peg.669 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00089 Genomic DNA Translation: AAB95884.1 U43738 Genomic DNA Translation: AAC43651.1 |
PIRi | S73562 |
RefSeqi | NP_110295.1, NC_000912.1 WP_010874963.1, NC_000912.1 |
3D structure databases
SMRi | P75189 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P75189, 6 interactors |
STRINGi | 272634.MPN_606 |
Genome annotation databases
EnsemblBacteriai | AAB95884; AAB95884; MPN_606 |
GeneIDi | 66608709 |
KEGGi | mpn:MPN_606 |
PATRICi | fig|272634.6.peg.669 |
Phylogenomic databases
HOGENOMi | CLU_031223_2_1_14 |
OMAi | EFMIIPV |
Enzyme and pathway databases
UniPathwayi | UPA00109;UER00187 |
Family and domain databases
CDDi | cd03313, enolase, 1 hit |
Gene3Di | 3.20.20.120, 1 hit 3.30.390.10, 1 hit |
HAMAPi | MF_00318, Enolase, 1 hit |
InterProi | View protein in InterPro IPR000941, Enolase IPR036849, Enolase-like_C_sf IPR029017, Enolase-like_N IPR020810, Enolase_C IPR020809, Enolase_CS IPR020811, Enolase_N |
PANTHERi | PTHR11902, PTHR11902, 1 hit |
Pfami | View protein in Pfam PF00113, Enolase_C, 1 hit PF03952, Enolase_N, 1 hit |
PIRSFi | PIRSF001400, Enolase, 1 hit |
PRINTSi | PR00148, ENOLASE |
SFLDi | SFLDF00002, enolase, 1 hit |
SMARTi | View protein in SMART SM01192, Enolase_C, 1 hit SM01193, Enolase_N, 1 hit |
SUPFAMi | SSF51604, SSF51604, 1 hit SSF54826, SSF54826, 1 hit |
TIGRFAMsi | TIGR01060, eno, 1 hit |
PROSITEi | View protein in PROSITE PS00164, ENOLASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ENO_MYCPN | |
Accessioni | P75189Primary (citable) accession number: P75189 Secondary accession number(s): Q50324 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families