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UniProtKB - P75168 (PT1_MYCPN)
Protein
Phosphoenolpyruvate-protein phosphotransferase
Gene
ptsI
Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Functioni
General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr).
By similarityMiscellaneous
The reaction takes place in three steps, mediated by a phosphocarrier histidine residue located on the surface of the central domain. The two first partial reactions are catalyzed at an active site located on the N-terminal domain, and the third partial reaction is catalyzed at an active site located on the C-terminal domain. For catalytic turnover, the central domain swivels from the concave surface of the N-terminal domain to that of the C-terminal domain.By similarity
Catalytic activityi
- L-histidyl-[protein] + phosphoenolpyruvate = Nπ-phospho-L-histidyl-[protein] + pyruvateBy similarityEC:2.7.3.9By similarity
Cofactori
Mg2+By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 190 | Tele-phosphohistidine intermediateBy similarity | 1 | |
Binding sitei | 297 | PEPBy similarity | 1 | |
Binding sitei | 333 | PEPBy similarity | 1 | |
Metal bindingi | 427 | MagnesiumBy similarity | 1 | |
Metal bindingi | 451 | MagnesiumBy similarity | 1 | |
Binding sitei | 461 | PEPBy similarity | 1 | |
Active sitei | 498 | Proton donorBy similarity | 1 |
GO - Molecular functioni
- kinase activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- phosphoenolpyruvate-protein phosphotransferase activity Source: UniProtKB-EC
GO - Biological processi
- phosphoenolpyruvate-dependent sugar phosphotransferase system Source: UniProtKB-KW
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Phosphotransferase system, Sugar transport, Transport |
Ligand | Magnesium, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphoenolpyruvate-protein phosphotransferaseBy similarity (EC:2.7.3.9By similarity)Alternative name(s): Phosphotransferase system, enzyme IBy similarity |
Gene namesi | Name:ptsI Ordered Locus Names:MPN_627 ORF Names:MP215 |
Organismi | Mycoplasma pneumoniae (strain ATCC 29342 / M129) |
Taxonomic identifieri | 272634 [NCBI] |
Taxonomic lineagei | Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000147077 | 1 – 572 | Phosphoenolpyruvate-protein phosphotransferaseAdd BLAST | 572 |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
IntActi | P75168, 1 interactor |
STRINGi | 272634.MPN_627 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 450 – 451 | PEP bindingBy similarity | 2 |
Domaini
The N-terminal domain contains the HPr binding site, the central domain the pyrophosphate/phosphate carrier histidine, and the C-terminal domain the pyruvate binding site.By similarity
Sequence similaritiesi
Belongs to the PEP-utilizing enzyme family.Curated
Phylogenomic databases
HOGENOMi | CLU_007308_7_0_14 |
OMAi | CNAEWAL |
Family and domain databases
Gene3Di | 1.10.274.10, 1 hit 3.20.20.60, 1 hit |
InterProi | View protein in InterPro IPR008279, PEP-util_enz_mobile_dom IPR018274, PEP_util_AS IPR000121, PEP_util_C IPR023151, PEP_util_CS IPR036637, Phosphohistidine_dom_sf IPR024692, PTS_EI IPR006318, PTS_EI-like IPR008731, PTS_EIN IPR036618, PtsI_HPr-bd_sf IPR015813, Pyrv/PenolPyrv_Kinase-like_dom IPR040442, Pyrv_Kinase-like_dom_sf |
Pfami | View protein in Pfam PF05524, PEP-utilisers_N, 1 hit PF00391, PEP-utilizers, 1 hit PF02896, PEP-utilizers_C, 1 hit |
PIRSFi | PIRSF000732, PTS_enzyme_I, 1 hit |
SUPFAMi | SSF47831, SSF47831, 1 hit SSF51621, SSF51621, 1 hit SSF52009, SSF52009, 1 hit |
TIGRFAMsi | TIGR01417, PTS_I_fam, 1 hit |
PROSITEi | View protein in PROSITE PS00742, PEP_ENZYMES_2, 1 hit PS00370, PEP_ENZYMES_PHOS_SITE, 1 hit |
i Sequence
Sequence statusi: Complete.
P75168-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKLSGIGVS DGMALAKAFL VKTPEFAVNK YLKHQLTKAQ AKRLLDSAFK
60 70 80 90 100
KAVKDLEEIK EITVNNINTE AGMIFDAHIQ MLNDPTITEQ LEQQLAQNVH
110 120 130 140 150
PVIAVDTVFS QTATMFSQMQ DKYFQERAAD ILDLRQRLLA YLTGQKPHDL
160 170 180 190 200
VKIKSDVIIV AHDLTPSQTA TLNKKYVKGF LTEIGGRTSH AAIMARSLEI
210 220 230 240 250
PAVVGIKGIT TKVKDGQIVG VDGRKGIAGL DLNSKDTTEW KKQKALEEKY
260 270 280 290 300
QQELKQYTNK ETVTLDGHAV VVAANIGNVK DMELACQYNT NGVGLFRTEF
310 320 330 340 350
LYMNSQEWPD EETQYQAYKA VLEQAHGDLV IIRTLDIGGD KKLNYYEFPH
360 370 380 390 400
EDNPFLGYRA LRLTLDKQDI FKTQLRALLR AADHGQLGIM FPMVATLDEL
410 420 430 440 450
LQAKQLLNQV HQELGGNKQF KLGIMIEIPA AVLAANTLSH HVDFFSIGTN
460 470 480 490 500
DLIQYSFAAD RMNKNVSYLY QPLNPALLKL IYLTIEGGKV NDIWTGMCGE
510 520 530 540 550
MAGEPLAIPL LLGLGLKEFS MSASSMFKAR MIIAKLNYTE CQTLAQKALT
560 570
LANAKEVEKL VEKFFKKKDI FI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00089 Genomic DNA Translation: AAB95863.1 |
PIRi | S73541 |
RefSeqi | NP_110316.1, NC_000912.1 WP_010874984.1, NC_000912.1 |
Genome annotation databases
EnsemblBacteriai | AAB95863; AAB95863; MPN_627 |
KEGGi | mpn:MPN_627 |
PATRICi | fig|272634.6.peg.691 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00089 Genomic DNA Translation: AAB95863.1 |
PIRi | S73541 |
RefSeqi | NP_110316.1, NC_000912.1 WP_010874984.1, NC_000912.1 |
3D structure databases
SMRi | P75168 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P75168, 1 interactor |
STRINGi | 272634.MPN_627 |
Genome annotation databases
EnsemblBacteriai | AAB95863; AAB95863; MPN_627 |
KEGGi | mpn:MPN_627 |
PATRICi | fig|272634.6.peg.691 |
Phylogenomic databases
HOGENOMi | CLU_007308_7_0_14 |
OMAi | CNAEWAL |
Family and domain databases
Gene3Di | 1.10.274.10, 1 hit 3.20.20.60, 1 hit |
InterProi | View protein in InterPro IPR008279, PEP-util_enz_mobile_dom IPR018274, PEP_util_AS IPR000121, PEP_util_C IPR023151, PEP_util_CS IPR036637, Phosphohistidine_dom_sf IPR024692, PTS_EI IPR006318, PTS_EI-like IPR008731, PTS_EIN IPR036618, PtsI_HPr-bd_sf IPR015813, Pyrv/PenolPyrv_Kinase-like_dom IPR040442, Pyrv_Kinase-like_dom_sf |
Pfami | View protein in Pfam PF05524, PEP-utilisers_N, 1 hit PF00391, PEP-utilizers, 1 hit PF02896, PEP-utilizers_C, 1 hit |
PIRSFi | PIRSF000732, PTS_enzyme_I, 1 hit |
SUPFAMi | SSF47831, SSF47831, 1 hit SSF51621, SSF51621, 1 hit SSF52009, SSF52009, 1 hit |
TIGRFAMsi | TIGR01417, PTS_I_fam, 1 hit |
PROSITEi | View protein in PROSITE PS00742, PEP_ENZYMES_2, 1 hit PS00370, PEP_ENZYMES_PHOS_SITE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PT1_MYCPN | |
Accessioni | P75168Primary (citable) accession number: P75168 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 120 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families