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Entry version 122 (13 Nov 2019)
Sequence version 1 (01 Feb 1997)
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Protein

DNA polymerase

Gene

pol

Organism
Thermococcus fumicolans
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

PI-TfuI recognizes and cleaves a minimal sequence of 16 base pairs (bp) on supercoiled DNA with either Mn2+ or Mg2+ as cofactor. It cleaves linear DNA only with Mn2+ and requires a 19-bp minimal recognition sequence.
PI-TfuII is a highly active homing endonuclease using Mg2+ as cofactor. Its minimal recognition and cleavage site is 21 bp long either on linear or circular DNA substrates. Its endonuclease activity is strongly inhibited by the 3' digestion product, which remains bound to the enzyme after the cleavage reaction.

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Temperature dependencei

Optimum temperature is 70 degrees Celsius.

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase
Biological processDNA replication, Intron homing
LigandMagnesium, Manganese

Enzyme and pathway databases

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
2.7.7.7 6299

Protein family/group databases

Restriction enzymes and methylases database

More...
REBASEi
4500 PI-TfuI
4501 PI-TfuII

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
DNA polymerase (EC:2.7.7.7)
Alternative name(s):
Pol Tfu
Cleaved into the following 2 chains:
Endonuclease PI-TfuI (EC:3.1.-.-)
Alternative name(s):
Tfu pol-1 intein
Endonuclease PI-TfuII (EC:3.1.-.-)
Alternative name(s):
Tfu pol-2 intein
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:pol
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiThermococcus fumicolans
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri46540 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000073481 – 406DNA polymerase, 1st partAdd BLAST406
ChainiPRO_0000007349407 – 766Endonuclease PI-TfuIAdd BLAST360
ChainiPRO_0000007350767 – 900DNA polymerase, 2nd partAdd BLAST134
ChainiPRO_0000007351901 – 1282Endonuclease PI-TfuIIAdd BLAST382
ChainiPRO_00000073521283 – 1523DNA polymerase, 3rd partAdd BLAST241

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the two intervening regions (inteins) followed by peptide ligation.

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
P74918

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini524 – 665DOD-type homing endonuclease 1PROSITE-ProRule annotationAdd BLAST142
Domaini1047 – 1186DOD-type homing endonuclease 2PROSITE-ProRule annotationAdd BLAST140

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the DNA polymerase type-B family.Curated

Keywords - Domaini

Repeat

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.132.60, 1 hit
3.10.28.10, 2 hits
3.30.420.10, 1 hit
3.90.1600.10, 3 hits

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR006172 DNA-dir_DNA_pol_B
IPR006133 DNA-dir_DNA_pol_B_exonuc
IPR006134 DNA-dir_DNA_pol_B_multi_dom
IPR042087 DNA_pol_B_C
IPR023211 DNA_pol_palm_dom_sf
IPR003586 Hint_dom_C
IPR003587 Hint_dom_N
IPR036844 Hint_dom_sf
IPR027434 Homing_endonucl
IPR006142 INTEIN
IPR030934 Intein_C
IPR004042 Intein_endonuc
IPR006141 Intein_N
IPR004860 LAGLIDADG_2
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00136 DNA_pol_B, 2 hits
PF03104 DNA_pol_B_exo1, 2 hits
PF14528 LAGLIDADG_3, 2 hits

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR00379 INTEIN

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00305 HintC, 2 hits
SM00306 HintN, 2 hits
SM00486 POLBc, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF51294 SSF51294, 2 hits
SSF53098 SSF53098, 1 hit
SSF55608 SSF55608, 2 hits

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR01443 intein_Cterm, 2 hits
TIGR01445 intein_Nterm, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50818 INTEIN_C_TER, 2 hits
PS50819 INTEIN_ENDONUCLEASE, 2 hits
PS50817 INTEIN_N_TER, 2 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

P74918-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MILDTDYITE DGRPVIRVFK KENGEFKIEY DRDFEPYIYA LLKDDSAIED
60 70 80 90 100
VKKITASRHG TTVRVVRAGK VKKKFLGRPI EVWKLYFTHP QDVPAIRDKI
110 120 130 140 150
REHPAVVDIY EYDIPFAKRY LIDKGLIPME GDEELKMLAF DIETLYHEGE
160 170 180 190 200
EFAEGPILMI SYADEEGARV ITWKKIDLPY VDVVSTEKEM IKRFLKVVKE
210 220 230 240 250
KDPDVLITYN GDNFDFAYLK KRSEKLGVKF ILGRDGSEPK IQRMGDRFAV
260 270 280 290 300
EVKGRIHFDL YPVIRHTINL PTYTLEAVYE AIFGQPKEKV YAEEIAQAWE
310 320 330 340 350
TGEGLERVAR YSMEDAKVTY ELGREFFPME AQLSRLVGQS FWDVSRSSTG
360 370 380 390 400
NLVEWYLLRK AYERNELAPN KPSGRELERR RGGYAGGYVK EPERGLWENI
410 420 430 440 450
AYLDFRCHPA DTKVIVKGKG VVNISEVREG DYVLGIDGWQ KVQRVWEYDY
460 470 480 490 500
EGELVNINGL KCTPNHKLPV VRRTERQTAI RDSLAKSFLT KKVKGKLITT
510 520 530 540 550
PLFEKIGKIE REDVPEEEIL KGELAGIILA EGTLLRKDVE YFDSSRGKKR
560 570 580 590 600
VSHQYRVEIT VGAQEEDFQR RIVYIFERLF GVTPSVYRKK NTNAITFKVA
610 620 630 640 650
KKEVYLRVRE IMDGIENLHA PSVLRGFFEG DGSVNKVRKT VVVNQGTNNE
660 670 680 690 700
WKIEVVSKLL NKLGIPHRRY TYDYTEREKT MTTHILEIAG RDGLILFQTI
710 720 730 740 750
VGFISTEKNM ALEEAIRNRE VNRLENNAFY TLADFTAKTE YYKGKVYDLT
760 770 780 790 800
LEGTPYYFAN GILTHNSLYP SIIISHNVSP DTLNREGCGE YDEAPQVGHR
810 820 830 840 850
FCKDFPGFIP SLLGDLLDER QKVKKHMKAT VDPIEKKLLD YRQRAIKILA
860 870 880 890 900
NSFYGYYGYA KARWYCKECA ESVTAWGRQY IETTMREIEE KFGFKVLYAD
910 920 930 940 950
SVTGDTEVTI RRNGRIEFVP IEKLFERVDH RVGEKEYCVL GGVEALTLDN
960 970 980 990 1000
RGRLVWKKVP YVMRHKTDKR IYRVWFTNSW YLDVTEDHSL IGYLNTSKVK
1010 1020 1030 1040 1050
PGKPLKERLV EVKPEELGGK VKSLITPNRP IARTIKANPI AVKLWELIGL
1060 1070 1080 1090 1100
LVGDGNWGGQ SNWAKYYVGL SCGLDKAEIE RKVLNPLREA SVISNYYDKS
1110 1120 1130 1140 1150
KKGDVSILSK WLAGFMVKYF KDENGNKAIP SFMFNLPREY IEAFLRGLFS
1160 1170 1180 1190 1200
ADGTVSLRRG IPEIRLTSVN RELSDAVRKL LWLVGVSNSL FTETKPNRYL
1210 1220 1230 1240 1250
EKESGTHSIH VRIKNKHRFA DRIGFLIDRK STKLSENLGG HTNKKRAYKY
1260 1270 1280 1290 1300
DFDLVYPRKI EEITYDGYVY DIEVEGTHRF FANGILVHNT DGFFATIPGA
1310 1320 1330 1340 1350
DAETVKKKAR EFLNYINPKL PGLLELEYEG FYRRGFFVTK KKYAVIDEEG
1360 1370 1380 1390 1400
KITTRGLEIV RRDWSEVAKE TQARVLEAIL RHGDVEEAVR IVKEVTEKLS
1410 1420 1430 1440 1450
KYEVPPEKLV IHEQITRELK DYKATGPHVA IAKRLAARGI KVRPGTVISY
1460 1470 1480 1490 1500
IVLKGSGRIG DRTIPFDEFD PTKHRYDAEY YIENQVLPAV ERILKAFGYK
1510 1520
KEDLRYQKTR QVGLGAWLKM GKK
Length:1,523
Mass (Da):175,918
Last modified:February 1, 1997 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i7A2AC8236BF2E5F5
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
Z69882 Genomic DNA Translation: CAA93738.1

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z69882 Genomic DNA Translation: CAA93738.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein family/group databases

REBASEi4500 PI-TfuI
4501 PI-TfuII

Proteomic databases

PRIDEiP74918

Enzyme and pathway databases

BRENDAi2.7.7.7 6299

Family and domain databases

Gene3Di1.10.132.60, 1 hit
3.10.28.10, 2 hits
3.30.420.10, 1 hit
3.90.1600.10, 3 hits
InterProiView protein in InterPro
IPR006172 DNA-dir_DNA_pol_B
IPR006133 DNA-dir_DNA_pol_B_exonuc
IPR006134 DNA-dir_DNA_pol_B_multi_dom
IPR042087 DNA_pol_B_C
IPR023211 DNA_pol_palm_dom_sf
IPR003586 Hint_dom_C
IPR003587 Hint_dom_N
IPR036844 Hint_dom_sf
IPR027434 Homing_endonucl
IPR006142 INTEIN
IPR030934 Intein_C
IPR004042 Intein_endonuc
IPR006141 Intein_N
IPR004860 LAGLIDADG_2
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PfamiView protein in Pfam
PF00136 DNA_pol_B, 2 hits
PF03104 DNA_pol_B_exo1, 2 hits
PF14528 LAGLIDADG_3, 2 hits
PRINTSiPR00379 INTEIN
SMARTiView protein in SMART
SM00305 HintC, 2 hits
SM00306 HintN, 2 hits
SM00486 POLBc, 1 hit
SUPFAMiSSF51294 SSF51294, 2 hits
SSF53098 SSF53098, 1 hit
SSF55608 SSF55608, 2 hits
TIGRFAMsiTIGR01443 intein_Cterm, 2 hits
TIGR01445 intein_Nterm, 1 hit
PROSITEiView protein in PROSITE
PS50818 INTEIN_C_TER, 2 hits
PS50819 INTEIN_ENDONUCLEASE, 2 hits
PS50817 INTEIN_N_TER, 2 hits

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiDPOL_THEFM
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P74918
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 1, 1997
Last modified: November 13, 2019
This is version 122 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families
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