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UniProtKB - P74879 (PDUW_SALTY)
Protein
Propionate kinase PduW
Gene
pduW
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Functioni
Works with phosphate acetyltransferase (pta) to capture exogenous propionate and regenerate propionyl-CoA during degradation of propionate and 1,2-propanediol (1,2-PD). Ectopic expression partially complements a cobB deletion allowing some growth on propionate (PubMed:12700259).
Restores growth to an eutQ deletion on ethanolamine and tetrathionate under anoxic conditions (PubMed:26448059).
2 PublicationsCaution
Was originally (Ref. 1) thought to be AckA.Curated
Catalytic activityi
- EC:2.7.2.15UniRule annotation1 Publication
: 1,2-propanediol degradation Pathwayi
This protein is involved in the pathway 1,2-propanediol degradation, which is part of Polyol metabolism.1 Publication1 PublicationView all proteins of this organism that are known to be involved in the pathway 1,2-propanediol degradation and in Polyol metabolism.
Pathwayi: propanoate degradation
This protein is involved in the pathway propanoate degradation, which is part of Organic acid metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway propanoate degradation and in Organic acid metabolism.
GO - Molecular functioni
- acetate kinase activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- propionate kinase activity Source: UniProtKB-UniRule
GO - Biological processi
- acetate metabolic process Source: GO_Central
- propanediol catabolic process Source: InterPro
- propionate catabolic process Source: InterPro
Keywordsi
Molecular function | Kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
UniPathwayi | UPA00621 UPA00946 |
Names & Taxonomyi
Protein namesi | Recommended name: Propionate kinase PduW1 PublicationUniRule annotation (EC:2.7.2.15UniRule annotation1 Publication) |
Gene namesi | Name:pduW1 PublicationUniRule annotation Ordered Locus Names:STM2057 |
Organismi | Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) |
Taxonomic identifieri | 99287 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Salmonella › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation1 Publication
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Decreased propionate kinase activity, cells grow to lower density on 1,2-PD and excrete more propionate into the medium. A double pduW-pduX deletion grows as well as wild-type on 1,2-PD.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000107651 | 1 – 404 | Propionate kinase PduWAdd BLAST | 404 |
Proteomic databases
PaxDbi | P74879 |
Expressioni
Inductioni
The BMC operon and BMC production is induced by growth on 1,2-PD vitamin B12 medium.1 Publication
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
HOGENOMi | CLU_020352_0_1_6 |
OMAi | PLPWRYY |
PhylomeDBi | P74879 |
Family and domain databases
HAMAPi | MF_00020, Acetate_kinase, 1 hit MF_01882, Propion_kin_subfam2, 1 hit |
InterProi | View protein in InterPro IPR004372, Ac/propionate_kinase IPR000890, Aliphatic_acid_kin_short-chain IPR023865, Aliphatic_acid_kinase_CS IPR043129, ATPase_NBD IPR024896, Propionate_kinase_PduW |
PANTHERi | PTHR21060, PTHR21060, 1 hit |
Pfami | View protein in Pfam PF00871, Acetate_kinase, 1 hit |
PIRSFi | PIRSF000722, Acetate_prop_kin, 1 hit |
PRINTSi | PR00471, ACETATEKNASE |
SUPFAMi | SSF53067, SSF53067, 2 hits |
TIGRFAMsi | TIGR00016, ackA, 1 hit |
PROSITEi | View protein in PROSITE PS01075, ACETATE_KINASE_1, 1 hit PS01076, ACETATE_KINASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P74879-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSYKIMAINA GSSSLKFQLL EMPQGDMLCQ GLIERIGMAD AQVTIKTHSQ
60 70 80 90 100
KWQETVPVAD HRDAVTLLLE KLLGYQIINS LRDIDGVGHR VAHGGEFFKD
110 120 130 140 150
STLVTDETLA QIERLAELAP LHNPVNALGI HVFRQLLPDA PSVAVFDTAF
160 170 180 190 200
HQTLDEPAYI YPLPWHYYAE LGIRRYGFHG TSHKYVSGVL AEKLGVPLSA
210 220 230 240 250
LRVICCHLGN GSSICAIKNG RSVNTSMGFT PQSGVMMGTR SGDIDPSILP
260 270 280 290 300
WIAQRESKTP QQLNQLLNNE SGLLGVSGVS SDYRDVEQAA NTGNRQAKLA
310 320 330 340 350
LTLFAERIRA TIGSYIMQMG GLDALVFTGG IGENSARARS AVCHNLQFLG
360 370 380 390 400
LAVDEEKNQR NATFIQTENA LVKVAVINTN EELMIAQDVM RIALPATEGL
CVPA
Sequence cautioni
The sequence AAD39021 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 25 | G → R in AAB09552 (Ref. 1) Curated | 1 | |
Sequence conflicti | 60 – 67 | DHRDAVTL → RSSRCVNW in AAB09552 (Ref. 1) Curated | 8 | |
Sequence conflicti | 92 | A → R in AAB09552 (Ref. 1) Curated | 1 | |
Sequence conflicti | 212 | S → T in AAB09552 (Ref. 1) Curated | 1 | |
Sequence conflicti | 247 – 261 | SILPW…SKTPQ → HSAVDSPARNENAE in AAB09552 (Ref. 1) CuratedAdd BLAST | 15 | |
Sequence conflicti | 300 – 309 | ALTLFAERIR → RSHSVCRTHS in AAB09552 (Ref. 1) Curated | 10 | |
Sequence conflicti | 342 – 352 | VCHNLQFLGLA → CAIISIFGLS in AAB09552 (Ref. 1) CuratedAdd BLAST | 11 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U67435 Genomic DNA Translation: AAB09552.1 AF026270 Genomic DNA Translation: AAD39021.1 Different initiation. AE006468 Genomic DNA Translation: AAL20961.1 |
RefSeqi | NP_461002.1, NC_003197.2 WP_000120647.1, NC_003197.2 |
Genome annotation databases
EnsemblBacteriai | AAL20961; AAL20961; STM2057 |
GeneIDi | 1253578 |
KEGGi | stm:STM2057 |
PATRICi | fig|99287.12.peg.2179 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U67435 Genomic DNA Translation: AAB09552.1 AF026270 Genomic DNA Translation: AAD39021.1 Different initiation. AE006468 Genomic DNA Translation: AAL20961.1 |
RefSeqi | NP_461002.1, NC_003197.2 WP_000120647.1, NC_003197.2 |
3D structure databases
AlphaFoldDBi | P74879 |
SMRi | P74879 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 99287.STM2057 |
Proteomic databases
PaxDbi | P74879 |
Genome annotation databases
EnsemblBacteriai | AAL20961; AAL20961; STM2057 |
GeneIDi | 1253578 |
KEGGi | stm:STM2057 |
PATRICi | fig|99287.12.peg.2179 |
Phylogenomic databases
HOGENOMi | CLU_020352_0_1_6 |
OMAi | PLPWRYY |
PhylomeDBi | P74879 |
Enzyme and pathway databases
UniPathwayi | UPA00621 UPA00946 |
Family and domain databases
HAMAPi | MF_00020, Acetate_kinase, 1 hit MF_01882, Propion_kin_subfam2, 1 hit |
InterProi | View protein in InterPro IPR004372, Ac/propionate_kinase IPR000890, Aliphatic_acid_kin_short-chain IPR023865, Aliphatic_acid_kinase_CS IPR043129, ATPase_NBD IPR024896, Propionate_kinase_PduW |
PANTHERi | PTHR21060, PTHR21060, 1 hit |
Pfami | View protein in Pfam PF00871, Acetate_kinase, 1 hit |
PIRSFi | PIRSF000722, Acetate_prop_kin, 1 hit |
PRINTSi | PR00471, ACETATEKNASE |
SUPFAMi | SSF53067, SSF53067, 2 hits |
TIGRFAMsi | TIGR00016, ackA, 1 hit |
PROSITEi | View protein in PROSITE PS01075, ACETATE_KINASE_1, 1 hit PS01076, ACETATE_KINASE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PDUW_SALTY | |
Accessioni | P74879Primary (citable) accession number: P74879 Secondary accession number(s): Q9XDM5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | May 30, 2000 | |
Last modified: | May 25, 2022 | |
This is version 118 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families