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Protein

DNA polymerase III subunit alpha

Gene

dnaE-N

more
Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase (By similarity).By similarity

Miscellaneous

The intein is a split intein capable of protein trans-splicing. The N- and C-terminal halves of dnaE are encoded by two separate genes located 745 kb apart in the genome and on opposite DNA strands. The dnaE-N product consists of a N-extein sequence followed by a 123-aa intein sequence (Ssp dnaE1), whereas the dnaE-C product consists of a 36-aa intein sequence (Ssp dnaE2) followed by a C-extein sequence. The N- and C-extein sequences together reconstitute a complete DnaE sequence that is interrupted by the intein sequences inside the beta- and tau-binding domains.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-directed DNA polymerase, Nucleotidyltransferase, Transferase
Biological processDNA replication

Protein family/group databases

MEROPSiN10.003

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase III subunit alpha (EC:2.7.7.7)
Cleaved into the following chain:
Gene namesi
Name:dnaE-N
Ordered Locus Names:slr0603
AND
Name:dnaE-C
Ordered Locus Names:sll1572
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000073641 – 774DNA polymerase III subunit alpha, 1st partAdd BLAST774
ChainiPRO_0000007365775 – 897Ssp dnaE intein, 1st partAdd BLAST123
ChainiPRO_0000007366898 – 933Ssp dnaE intein, 2nd partAdd BLAST36
ChainiPRO_0000007367934 – 1355DNA polymerase III subunit alpha, 2nd partAdd BLAST422

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Proteomic databases

PaxDbiP74750
PRIDEiP74750

Interactioni

Subunit structurei

DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the PolIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex (By similarity).By similarity

Protein-protein interaction databases

IntActiP74750, 2 interactors
STRINGi1148.SYNGTS_3164

Structurei

Secondary structure

11355
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi781 – 784Combined sources4
Turni785 – 787Combined sources3
Beta strandi788 – 791Combined sources4
Helixi792 – 797Combined sources6
Beta strandi803 – 807Combined sources5
Beta strandi811 – 817Combined sources7
Beta strandi820 – 834Combined sources15
Beta strandi839 – 842Combined sources4
Beta strandi847 – 850Combined sources4
Beta strandi855 – 857Combined sources3
Helixi858 – 864Combined sources7
Beta strandi868 – 871Combined sources4
Beta strandi877 – 879Combined sources3
Turni883 – 885Combined sources3
Beta strandi887 – 889Combined sources3
Helixi890 – 893Combined sources4
Beta strandi896 – 899Combined sources4
Beta strandi901 – 916Combined sources16
Beta strandi923 – 925Combined sources3
Beta strandi928 – 932Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZD7X-ray1.70A/B775-933[»]
1ZDEX-ray1.95A770-936[»]
3NZMX-ray1.55A772-933[»]
4GIGX-ray1.80A775-933[»]
5OL5X-ray2.33A/B/C/D776-931[»]
ProteinModelPortaliP74750
SMRiP74750
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP74750

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000021784
InParanoidiP74750
KOiK02337
PhylomeDBiP74750

Family and domain databases

InterProiView protein in InterPro
IPR011708 DNA_pol3_alpha
IPR029460 DNAPol_HHH
IPR003587 Hint_dom_N
IPR036844 Hint_dom_sf
IPR030934 Intein_C
IPR006141 Intein_N
IPR004365 NA-bd_OB_tRNA
IPR004013 PHP_dom
IPR003141 Pol/His_phosphatase_N
IPR016195 Pol/histidinol_Pase-like
IPR004805 PolC_alpha
PANTHERiPTHR32294 PTHR32294, 1 hit
PfamiView protein in Pfam
PF07733 DNA_pol3_alpha, 1 hit
PF14579 HHH_6, 1 hit
PF02811 PHP, 1 hit
PF01336 tRNA_anti-codon, 1 hit
SMARTiView protein in SMART
SM00306 HintN, 1 hit
SM00481 POLIIIAc, 1 hit
SUPFAMiSSF51294 SSF51294, 1 hit
SSF89550 SSF89550, 1 hit
TIGRFAMsiTIGR01443 intein_Cterm, 1 hit
TIGR01445 intein_Nterm, 1 hit
TIGR00594 polc, 1 hit
PROSITEiView protein in PROSITE
PS50818 INTEIN_C_TER, 1 hit
PS50817 INTEIN_N_TER, 1 hit

Sequencei

Sequence statusi: Fragments.

Sequence processingi: The displayed sequence is further processed into a mature form.

P74750-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFVGLHIHS DYSLLDGASQ LPALIDRAIE LGMPAIALTD HGVMYGAVEL
60 70 80 90 100
LKVCRGKPIK PIIGNEMYVI NGDIEINKRH RRFHQVVLAK NNQGYRNLVK
110 120 130 140 150
LTTISNLKGI QGSGIFARPC INKELLEQYK EGLIVTSACL GGEVPQAILK
160 170 180 190 200
GDLDHARQVA KWYKNLFGDD YYLEIQDHGS VEDRLVNINL VKIAQELDIK
210 220 230 240 250
IVATNDSHFI SCNDVEAHDA LLCIQTGKLI TEEKRLRYSG TEYLKSAEEM
260 270 280 290 300
HLLFRDHLPD DIIETAIANT LEVADKVEAY SILGEPRIPH YPIPPNHTPE
310 320 330 340 350
TYVEDIAWDG LLERLKCRER ADITPVYKER LEYELRMLEK MGFCTYFLVV
360 370 380 390 400
WDYIKYARDH GIPVGPGRGS AAGSLVAYCM KITNIDPVHH GLIFERFLNP
410 420 430 440 450
ERKSMPDVDT DFCIDRRDEM IEYVTQKYGE DKVAQIITFN RMTSKAVLKD
460 470 480 490 500
VARVLDIPYA ESDKMAKMIP VSRGKPAKLK VMISDQTPEP QFKARYDNEP
510 520 530 540 550
WVKKWIDMAI RIEGTNKTFG VHAAGVVISS EPLDQIVPLQ KNNDGAVITQ
560 570 580 590 600
YYMEDVEAMG LLKMDFLGLK NLTTLQRAVE LVAETKGIEL DLDDLPLQER
610 620 630 640 650
KALQIRARTG SKKLPDDVKK THKLLEAGDL EGIFQLESQG MKQIVRDLKP
660 670 680 690 700
SGIEDISSIL ALYRPGPLDA GLIPIFINRK HGREEISYQH KLLEPILNET
710 720 730 740 750
YGVLVYQEQI MKMAQDLADY SLGEADLLRR AMGKKKAEEM QKHRAKFVDG
760 770 780 790 800
STKHGVPSRI AENLFDQMVK FAEYCLSFGT EILTVEYGPL PIGKIVSEEI
810 820 830 840 850
NCSVYSVDPE GRVYTQAIAQ WHDRGEQEVL EYELEDGSVI RATSDHRFLT
860 870 880 890 900
TDYQLLAIEE IFARQLDLLT LENIKQTEEA LDNHRLPFPL LDAGTIKMVK
910 920 930 940 950
VIGRRSLGVQ RIFDIGLPQD HNFLLANGAI AANCFNKSHS TAYAYVTYQT
960 970 980 990 1000
AYLKANYPVE YMAALLTASS DSQEKVEKYR ENCQKMGITV EPPDINRSQR
1010 1020 1030 1040 1050
HFTPLGEAIL FGLSAVRNLG EGAIEQIITA RDNSEEKRFK SLADFCTQVD
1060 1070 1080 1090 1100
LRVVNRRAIE TLIMAGAFDG VQSNRNQLLH DLELVIAWAQ KRAKEKETGQ
1110 1120 1130 1140 1150
LNIFDSLTAG ESIKAKEAAN NGFEQEPSAP PVAEFSLQEK LQLEKEHLGF
1160 1170 1180 1190 1200
YVSEHPLKSV QRSARLLSPI NLVDLDQYKV RQKVSAVAIL VAVKKIITKK
1210 1220 1230 1240 1250
NGQPMAFLTL EDMSGQSEAV VFPSNYERLQ DVLIEGSQQM IWGKVDRRDD
1260 1270 1280 1290 1300
QYQLIVEDLE PVEEVKMVML DLTPQEIANT STQARLKQIL QSHAPQKEQM
1310 1320 1330 1340 1350
KIPVVACINE GPTKHFIRFG ENYWVNDYGT AVASLQELGF RARLEPLLPQ

SQSVG
Length:1,355
Mass (Da):153,001
Last modified:September 26, 2001 - v2
Checksum:i7C4F61EE4C36EE0F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-adjacent residuesi897 – 898Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA Translation: BAA18870.1 Sequence problems.
BA000022 Genomic DNA Translation: BAA17242.1 Sequence problems.
PIRiA59016
S75328
S76958

Genome annotation databases

EnsemblBacteriaiBAA17242; BAA17242; BAA17242
BAA18870; BAA18870; BAA18870
KEGGisyn:sll1572
syn:slr0603

Similar proteinsi

Entry informationi

Entry nameiDPO3A_SYNY3
AccessioniPrimary (citable) accession number: P74750
Secondary accession number(s): P73215
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: September 26, 2001
Last modified: March 28, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health