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UniProtKB - P72848 (HEM6_SYNY3)
Protein
Oxygen-dependent coproporphyrinogen-III oxidase
Gene
hemF
Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Functioni
Involved in the heme and chlorophyll biosynthesis. Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX.
UniRule annotation1 PublicationCatalytic activityi
- coproporphyrinogen III + 2 H+ + O2 = 2 CO2 + 2 H2O + protoporphyrinogen IXUniRule annotation1 PublicationEC:1.3.3.3UniRule annotation1 Publication
Cofactori
a divalent metal cation1 Publication
: protoporphyrin-IX biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes protoporphyrinogen-IX from coproporphyrinogen-III (O2 route).UniRule annotation This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoporphyrinogen-IX from coproporphyrinogen-III (O2 route), the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 109 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 113 | Divalent metal cationUniRule annotation | 1 | |
Active sitei | 123 | Proton donorUniRule annotation | 1 | |
Metal bindingi | 123 | Divalent metal cationUniRule annotation | 1 | |
Metal bindingi | 157 | Divalent metal cationUniRule annotation | 1 | |
Metal bindingi | 187 | Divalent metal cationUniRule annotation | 1 | |
Sitei | 187 | Important for dimerizationUniRule annotation | 1 |
GO - Molecular functioni
- coproporphyrinogen oxidase activity Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- protein homodimerization activity Source: UniProtKB
GO - Biological processi
- chlorophyll biosynthetic process Source: UniProtKB-UniRule
- protoporphyrinogen IX biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Chlorophyll biosynthesis, Heme biosynthesis, Porphyrin biosynthesis |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
BRENDAi | 1.3.3.3, 382 |
UniPathwayi | UPA00251;UER00322 |
Names & Taxonomyi
Protein namesi | Recommended name: Oxygen-dependent coproporphyrinogen-III oxidaseUniRule annotation (EC:1.3.3.3UniRule annotation)Short name: CPOUniRule annotation Short name: Coprogen oxidaseUniRule annotation Short name: CoproporphyrinogenaseUniRule annotation |
Gene namesi | Name:hemFUniRule annotation Ordered Locus Names:sll1185 |
Organismi | Synechocystis sp. (strain PCC 6803 / Kazusa) |
Taxonomic identifieri | 1111708 [NCBI] |
Taxonomic lineagei | Bacteria › Cyanobacteria › Synechococcales › Merismopediaceae › Synechocystis › unclassified Synechocystis › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Cells lacking this gene fail to grow under aerobic conditions, with accumulation of coproporphyrin-III.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000109925 | 1 – 340 | Oxygen-dependent coproporphyrinogen-III oxidaseAdd BLAST | 340 |
Proteomic databases
PaxDbi | P72848 |
Expressioni
Inductioni
Constitutively expressed.1 Publication
Interactioni
Subunit structurei
Homodimer.
UniRule annotationGO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
STRINGi | 1148.1651937 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 22 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 125 – 127 | Substrate bindingUniRule annotation | 3 | |
Regioni | 278 – 313 | Important for dimerizationUniRule annotationAdd BLAST | 36 | |
Regioni | 296 – 298 | Substrate bindingUniRule annotation | 3 |
Sequence similaritiesi
Belongs to the aerobic coproporphyrinogen-III oxidase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0408, Bacteria |
InParanoidi | P72848 |
OMAi | HDKGTLF |
PhylomeDBi | P72848 |
Family and domain databases
Gene3Di | 3.40.1500.10, 1 hit |
HAMAPi | MF_00333, Coprogen_oxidas, 1 hit |
InterProi | View protein in InterPro IPR001260, Coprogen_oxidase_aer IPR036406, Coprogen_oxidase_aer_sf IPR018375, Coprogen_oxidase_CS |
PANTHERi | PTHR10755, PTHR10755, 1 hit |
Pfami | View protein in Pfam PF01218, Coprogen_oxidas, 1 hit |
PIRSFi | PIRSF000166, Coproporphyri_ox, 1 hit |
PRINTSi | PR00073, COPRGNOXDASE |
SUPFAMi | SSF102886, SSF102886, 1 hit |
PROSITEi | View protein in PROSITE PS01021, COPROGEN_OXIDASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P72848-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTVSPTTQPQ TNHSLPPADA KQRVSQFMQT LQDEICQGLE ALDGKGKFQE
60 70 80 90 100
DSWQREEGGG GRSRVLADGD FLEQGGVNFS EVWGKSLPPS ILKQRPEAEG
110 120 130 140 150
HEFYATGTSM VLHPKNPYIP TVHLNYRYFE AGPVWWFGGG ADLTPYYPFA
160 170 180 190 200
EDAAHFHHTL KNACDQTHGE FYPVFKRWCD EYFYLKHRQE MRGIGGIFFD
210 220 230 240 250
YQDGNAPLYR GPDPNGPAAQ YSNQLAPIEP LGWEDLFSFA QRCGRAFLPA
260 270 280 290 300
YSPIVEKRRN TEYGDRQRQF QLYRRGRYVE FNLVYDRGTI FGLQTNGRTE
310 320 330 340
SILMSLPPLV RWQYCYSPEA GSPEAELTEK FLVPQDWVNS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BA000022 Genomic DNA Translation: BAA16863.1 |
PIRi | S74712 |
Genome annotation databases
EnsemblBacteriai | BAA16863; BAA16863; BAA16863 |
KEGGi | syn:sll1185 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BA000022 Genomic DNA Translation: BAA16863.1 |
PIRi | S74712 |
3D structure databases
AlphaFoldDBi | P72848 |
SMRi | P72848 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 1148.1651937 |
Proteomic databases
PaxDbi | P72848 |
Genome annotation databases
EnsemblBacteriai | BAA16863; BAA16863; BAA16863 |
KEGGi | syn:sll1185 |
Phylogenomic databases
eggNOGi | COG0408, Bacteria |
InParanoidi | P72848 |
OMAi | HDKGTLF |
PhylomeDBi | P72848 |
Enzyme and pathway databases
UniPathwayi | UPA00251;UER00322 |
BRENDAi | 1.3.3.3, 382 |
Family and domain databases
Gene3Di | 3.40.1500.10, 1 hit |
HAMAPi | MF_00333, Coprogen_oxidas, 1 hit |
InterProi | View protein in InterPro IPR001260, Coprogen_oxidase_aer IPR036406, Coprogen_oxidase_aer_sf IPR018375, Coprogen_oxidase_CS |
PANTHERi | PTHR10755, PTHR10755, 1 hit |
Pfami | View protein in Pfam PF01218, Coprogen_oxidas, 1 hit |
PIRSFi | PIRSF000166, Coproporphyri_ox, 1 hit |
PRINTSi | PR00073, COPRGNOXDASE |
SUPFAMi | SSF102886, SSF102886, 1 hit |
PROSITEi | View protein in PROSITE PS01021, COPROGEN_OXIDASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HEM6_SYNY3 | |
Accessioni | P72848Primary (citable) accession number: P72848 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 131 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families