Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Entry version 72 (02 Dec 2020)
Sequence version 1 (01 Feb 1997)
Previous versions | rss
Add a publicationFeedback
Protein

2-oxoacid:ferredoxin oxidoreductase subunit alpha

Gene
N/A
Organism
Sulfolobus sp.
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.

3 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

Kcat is 51 sec(-1) for pyruvate as substrate (PubMed:11683888). Kcat is 19 sec(-1) for 2-oxoglutarate as substrate (PubMed:11683888).1 Publication
  1. KM=250 µM for pyruvate (at pH 6.8 and 50 degrees Celsius)1 Publication
  2. KM=280 µM for pyruvate1 Publication
  3. KM=480 µM for 2-oxobutyrate1 Publication
  4. KM=870 µM for 2-oxoglutarate (at pH 6.8 and 50 degrees Celsius)2 Publications
  1. Vmax=86 µmol/min/mg enzyme with 2-oxoglutarate as substrate (at pH 6.8 and 50 degrees Celsius)1 Publication
  2. Vmax=30 µmol/min/mg enzyme with pyruvate as substrate1 Publication
  3. Vmax=28 µmol/min/mg enzyme with 2-oxobutyrate as substrate1 Publication
  4. Vmax=11 µmol/min/mg enzyme with 2-oxoglutarate as substrate1 Publication

pH dependencei

Optimum pH is 8.5.1 Publication

Temperature dependencei

Optimum temperature is 90 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei256SubstrateBy similarity1
Binding sitei344SubstrateBy similarity1
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei353Plays a key role in the broad substrate specificity1 Publication1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionOxidoreductase
LigandPyruvate

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
MetaCyc:MONOMER-11911

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
1.2.7.11, 6164

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
2-oxoacid:ferredoxin oxidoreductase subunit alpha1 Publication (EC:1.2.7.113 Publications)
Short name:
OFOR1 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSulfolobus sp.
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri2288 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobusunclassified Sulfolobus

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi253Y → A or W: Loss of oxidoreductase activity. 1 Publication1
Mutagenesisi253Y → F: Significant decrease in the catalytic efficiency, while the affinity for 2-oxoacid is only slightly affected. 1 Publication1
Mutagenesisi254P → G: Significant decrease in the catalytic efficiency, while the affinity for 2-oxoacid is only slightly affected. 1 Publication1
Mutagenesisi255I → L, M, S or V: Significant decrease in the catalytic efficiency, while the affinity for 2-oxoacid is only slightly affected. 1 Publication1
Mutagenesisi256T → A, S or V: Significant decrease in the catalytic efficiency, while the affinity for 2-oxoacid is only slightly affected. 2 Publications1
Mutagenesisi257P → A, G or V: Loss of oxidoreductase activity. 1 Publication1
Mutagenesisi344R → A or K: Loss of oxidoreductase activity. 1 Publication1
Mutagenesisi353T → I or V: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004455291 – 6322-oxoacid:ferredoxin oxidoreductase subunit alphaAdd BLAST632

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Heterodimer composed of an alpha and a beta subunit.

3 Publications

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P72578

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi253 – 257YPITP motif1 Publication5

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The Tyr-Pro-Ile-Thr-Pro (YPITP) motif is important for the turnover of the reaction, presumably through its flexibility and mobility.1 Publication

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.40.50.920, 1 hit
3.40.920.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR022367, 2-oxoacid/accept_OxRdtase_asu
IPR019752, Pyrv/ketoisovalerate_OxRed_cat
IPR002880, Pyrv_Fd/Flavodoxin_OxRdtase_N
IPR002869, Pyrv_flavodox_OxRed_cen
IPR029061, THDP-binding
IPR009014, Transketo_C/PFOR_II

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01558, POR, 1 hit
PF01855, POR_N, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF52518, SSF52518, 1 hit
SSF52922, SSF52922, 1 hit
SSF53323, SSF53323, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR03710, OAFO_sf, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P72578-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRLSWVIGGA QGTGIDTAAN IFGNAVASAG YYIYGNREYY SNIKGGHSYF
60 70 80 90 100
SLTISDKRVR SNTQKIDILV SFDAETVFQH FYDVKDILIY NKAVETTKID
110 120 130 140 150
AVQSMEPELA ERIKDFLTKQ GYETTVKGAL EYASKNNVTL IPVNYDEIAK
160 170 180 190 200
KVADEMKVPL SVTERVKNIV GITISYKLLG LDVNYLIEAI NSTFKQDLYR
210 220 230 240 250
KMNELAVKDS YDIVESRYNL KPSSKERRRF WLDGNTAVAI GKIYGGVRFQ
260 270 280 290 300
SYYPITPASD ESVYIEAHQD VLMEDPITGD KKKGTIVVVQ AEDELAAINM
310 320 330 340 350
AIGAALTGVR AATATSGPGF SLMVEGLGWA GMNEVPVVIT YYIRGGPSTG
360 370 380 390 400
LPTRTAQSDL IFPIFAGHGE FPKIVLASGD HAEAFKDAIW ALNLAEKYQT
410 420 430 440 450
PVIHLVEKTL ANSYSTIPYE ELELDKLKAE RGKIVESGDI SYKRFKFTED
460 470 480 490 500
GISPRAFLGK ATMYYTGDEH NEEGHISEDV VNRTMMYEKR MKKLEVADKE
510 520 530 540 550
IPEESRVKIY GDLNSRNLII TWGSPTGVLR DILEESNFDF TLLQIRMFSP
560 570 580 590 600
FPKNLVSKLM EGRDKIITVE GNYLAQTSLL VKMYTGKDVT NSILKWNGRP
610 620 630
FLRDELEEAL IKVIKDGEKR VVLNGGIYTS ME
Length:632
Mass (Da):70,759
Last modified:February 1, 1997 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i362566F1D59D72D9
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
D64024 Genomic DNA Translation: BAA10898.1

Protein sequence database of the Protein Information Resource

More...
PIRi
JC4919

Genome annotation databases

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ag:BAA10898

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D64024 Genomic DNA Translation: BAA10898.1
PIRiJC4919

3D structure databases

SMRiP72578
ModBaseiSearch...

Genome annotation databases

KEGGiag:BAA10898

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-11911
BRENDAi1.2.7.11, 6164

Family and domain databases

Gene3Di3.40.50.920, 1 hit
3.40.920.10, 1 hit
InterProiView protein in InterPro
IPR022367, 2-oxoacid/accept_OxRdtase_asu
IPR019752, Pyrv/ketoisovalerate_OxRed_cat
IPR002880, Pyrv_Fd/Flavodoxin_OxRdtase_N
IPR002869, Pyrv_flavodox_OxRed_cen
IPR029061, THDP-binding
IPR009014, Transketo_C/PFOR_II
PfamiView protein in Pfam
PF01558, POR, 1 hit
PF01855, POR_N, 1 hit
SUPFAMiSSF52518, SSF52518, 1 hit
SSF52922, SSF52922, 1 hit
SSF53323, SSF53323, 1 hit
TIGRFAMsiTIGR03710, OAFO_sf, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiOFOA_SULSP
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P72578
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2018
Last sequence update: February 1, 1997
Last modified: December 2, 2020
This is version 72 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Direct protein sequencing
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again