UniProtKB - P72158 (PURK_PSEAE)
Protein
N5-carboxyaminoimidazole ribonucleotide synthase
Gene
purK
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Functioni
Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO3- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).UniRule annotation
Catalytic activityi
- 5-amino-1-(5-phospho-β-D-ribosyl)imidazole + ATP + hydrogencarbonate = 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + ADP + 2 H+ + phosphateUniRule annotationEC:6.3.4.18UniRule annotation
: IMP biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route).UniRule annotationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- N5-carboxyaminoimidazole ribonucleotide synthase (purK)
- N5-carboxyaminoimidazole ribonucleotide mutase (purE)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 98 | ATPUniRule annotation | 1 | |
Binding sitei | 138 | ATPUniRule annotation | 1 | |
Binding sitei | 181 | ATPUniRule annotation | 1 | |
Binding sitei | 204 | ATPUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 143 – 149 | ATPUniRule annotation | 7 | |
Nucleotide bindingi | 173 – 176 | ATPUniRule annotation | 4 | |
Nucleotide bindingi | 255 – 256 | ATPUniRule annotation | 2 |
GO - Molecular functioni
- 5-(carboxyamino)imidazole ribonucleotide synthase activity Source: UniProtKB-UniRule
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: InterPro
- phosphoribosylaminoimidazole carboxylase activity Source: InterPro
GO - Biological processi
- 'de novo' IMP biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Ligase |
Biological process | Purine biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | PAER208964:G1FZ6-5552-MONOMER |
UniPathwayi | UPA00074;UER00942 |
Names & Taxonomyi
Protein namesi | Recommended name: N5-carboxyaminoimidazole ribonucleotide synthaseUniRule annotation (EC:6.3.4.18UniRule annotation)Short name: N5-CAIR synthaseUniRule annotation Alternative name(s): 5-(carboxyamino)imidazole ribonucleotide synthetaseUniRule annotation |
Gene namesi | Name:purKUniRule annotation Ordered Locus Names:PA5425 |
Organismi | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
Taxonomic identifieri | 208964 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › |
Proteomesi |
|
Organism-specific databases
PseudoCAPi | PA5425 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000075002 | 1 – 360 | N5-carboxyaminoimidazole ribonucleotide synthaseAdd BLAST | 360 |
Proteomic databases
PaxDbi | P72158 |
PRIDEi | P72158 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 102 – 285 | ATP-graspUniRule annotationAdd BLAST | 184 |
Sequence similaritiesi
Belongs to the PurK/PurT family.UniRule annotation
Phylogenomic databases
HOGENOMi | CLU_011534_0_1_6 |
InParanoidi | P72158 |
OMAi | ITFDHEH |
PhylomeDBi | P72158 |
Family and domain databases
Gene3Di | 3.30.1490.20, 1 hit |
HAMAPi | MF_01928, PurK, 1 hit |
InterProi | View protein in InterPro IPR011761, ATP-grasp IPR003135, ATP-grasp_carboxylate-amine IPR013815, ATP_grasp_subdomain_1 IPR016185, PreATP-grasp_dom_sf IPR005875, PurK IPR040686, PurK_C IPR011054, Rudment_hybrid_motif |
Pfami | View protein in Pfam PF02222, ATP-grasp, 1 hit PF17769, PurK_C, 1 hit |
SUPFAMi | SSF51246, SSF51246, 1 hit SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR01161, purK, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit |
i Sequence
Sequence statusi: Complete.
P72158-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKIGVIGGGQ LGRMLALAGT PLGMNFAFLD PAPDACAASL GEHIRADYGD
60 70 80 90 100
QEHLRQLADE VDLVTFEFES VPAETVAFLS QFVPVYPNAE SLRIARDRWF
110 120 130 140 150
EKSMFKDLGI PTPDFADVQS QADLDAAAAA IGLPAVLKTR TLGYDGKGQK
160 170 180 190 200
VLRQPADVQG AFAELGSVPC ILEGFVPFTG EVSLVAVRAR DGETRFYPLV
210 220 230 240 250
HNTHDSGILK LSVASSGHPL QALAEDYVGR VLARLDYVGV LAFEFFEVDG
260 270 280 290 300
GLKANEIAPR VHNSGHWTIE GAECSQFENH LRAVAGLPLG STAKVGESAM
310 320 330 340 350
LNFIGAVPPV AQVVAVADCH LHHYGKAFKN GRKVGHATLR CADRATLQAR
360
IAEVEALIEA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 124 | L → V in AAB17258 (PubMed:8816818).Curated | 1 | |
Sequence conflicti | 133 | L → V in AAB17258 (PubMed:8816818).Curated | 1 | |
Sequence conflicti | 196 | F → L in AAB17258 (PubMed:8816818).Curated | 1 | |
Sequence conflicti | 217 | G → A in AAB17258 (PubMed:8816818).Curated | 1 | |
Sequence conflicti | 353 | E → D in AAB17258 (PubMed:8816818).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U58364 Genomic DNA Translation: AAB17258.1 AE004091 Genomic DNA Translation: AAG08810.1 |
PIRi | G82967 |
RefSeqi | NP_254112.1, NC_002516.2 WP_003096798.1, NZ_QZGE01000012.1 |
Genome annotation databases
EnsemblBacteriai | AAG08810; AAG08810; PA5425 |
GeneIDi | 878440 |
KEGGi | pae:PA5425 |
PATRICi | fig|208964.12.peg.5685 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U58364 Genomic DNA Translation: AAB17258.1 AE004091 Genomic DNA Translation: AAG08810.1 |
PIRi | G82967 |
RefSeqi | NP_254112.1, NC_002516.2 WP_003096798.1, NZ_QZGE01000012.1 |
3D structure databases
SMRi | P72158 |
ModBasei | Search... |
Proteomic databases
PaxDbi | P72158 |
PRIDEi | P72158 |
Genome annotation databases
EnsemblBacteriai | AAG08810; AAG08810; PA5425 |
GeneIDi | 878440 |
KEGGi | pae:PA5425 |
PATRICi | fig|208964.12.peg.5685 |
Organism-specific databases
PseudoCAPi | PA5425 |
Phylogenomic databases
HOGENOMi | CLU_011534_0_1_6 |
InParanoidi | P72158 |
OMAi | ITFDHEH |
PhylomeDBi | P72158 |
Enzyme and pathway databases
UniPathwayi | UPA00074;UER00942 |
BioCyci | PAER208964:G1FZ6-5552-MONOMER |
Family and domain databases
Gene3Di | 3.30.1490.20, 1 hit |
HAMAPi | MF_01928, PurK, 1 hit |
InterProi | View protein in InterPro IPR011761, ATP-grasp IPR003135, ATP-grasp_carboxylate-amine IPR013815, ATP_grasp_subdomain_1 IPR016185, PreATP-grasp_dom_sf IPR005875, PurK IPR040686, PurK_C IPR011054, Rudment_hybrid_motif |
Pfami | View protein in Pfam PF02222, ATP-grasp, 1 hit PF17769, PurK_C, 1 hit |
SUPFAMi | SSF51246, SSF51246, 1 hit SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR01161, purK, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PURK_PSEAE | |
Accessioni | P72158Primary (citable) accession number: P72158 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | December 8, 2000 | |
Last modified: | December 2, 2020 | |
This is version 130 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families