UniProtKB - P71301 (ECPR_ECOLI)
Protein
HTH-type transcriptional regulator EcpR
Gene
ecpR
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the ecpRABCDE operon, which encodes the E.coli common pilus (ECP). ECP is found in both commensal and pathogenic strains and plays a dual role in early-stage biofilm development and host cell recognition. Positively regulates the expression of the ecp operon (By similarity). Also represses expression of the flagellar master operon flhDC, and consequently prevents flagellum biosynthesis and motility. Acts by binding to the regulatory region of the flhDC operon (Probable).By similarityCurated1 Publication
Miscellaneous
Expressed in strain K12.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 162 – 181 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
GO - Biological processi
- regulation of transcription, DNA-templated Source: InterPro
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | EcoCyc:G6165-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: HTH-type transcriptional regulator EcpR |
Gene namesi | Name:ecpR Synonyms:matA, ykgK Ordered Locus Names:b0294, JW5031 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm Curated
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 179 | H → P: Slightly increases motility. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000184199 | 1 – 196 | HTH-type transcriptional regulator EcpRAdd BLAST | 196 |
Proteomic databases
PaxDbi | P71301 |
PRIDEi | P71301 |
Expressioni
Inductioni
Negatively regulated by H-NS. Positively autoregulated. Also positively regulated by IHF (By similarity).By similarity
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259793, 6 interactors 849363, 1 interactor |
IntActi | P71301, 2 interactors |
STRINGi | 511145.b0294 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 138 – 196 | HTH luxR-typePROSITE-ProRule annotationAdd BLAST | 59 |
Sequence similaritiesi
Belongs to the EcpR/MatA family.Curated
Phylogenomic databases
eggNOGi | COG2771, Bacteria |
HOGENOMi | CLU_128111_0_0_6 |
Family and domain databases
CDDi | cd06170, LuxR_C_like, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR016032, Sig_transdc_resp-reg_C-effctor IPR000792, Tscrpt_reg_LuxR_C IPR036388, WH-like_DNA-bd_sf |
Pfami | View protein in Pfam PF00196, GerE, 1 hit |
PRINTSi | PR00038, HTHLUXR |
SMARTi | View protein in SMART SM00421, HTH_LUXR, 1 hit |
SUPFAMi | SSF46894, SSF46894, 1 hit |
PROSITEi | View protein in PROSITE PS50043, HTH_LUXR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P71301-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTWQSDYSRD YEVKNHMECQ NRSDKYIWSP HDAYFYKGLS ELIVDIDRLI
60 70 80 90 100
YLSLEKIRKD FVFINLSTDS LSEFINRDNE WLSAVKGKQV VLIAARKSEA
110 120 130 140 150
LANYWYYNSN IRGVVYAGLS RDIRKELVYV INGRFLRKDI KKDKITDREM
160 170 180 190
EIIRMTAQGM QPKSIARIEN CSVKTVYTHR RNAEAKLYSK IYKLVQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U73857 Genomic DNA Translation: AAB18023.1 U00096 Genomic DNA Translation: AAC73397.1 AP009048 Genomic DNA Translation: BAE76078.1 |
PIRi | F64755 |
RefSeqi | NP_414828.1, NC_000913.3 |
Genome annotation databases
EnsemblBacteriai | AAC73397; AAC73397; b0294 BAE76078; BAE76078; BAE76078 |
GeneIDi | 944966 |
KEGGi | ecj:JW5031 eco:b0294 |
PATRICi | fig|511145.12.peg.298 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U73857 Genomic DNA Translation: AAB18023.1 U00096 Genomic DNA Translation: AAC73397.1 AP009048 Genomic DNA Translation: BAE76078.1 |
PIRi | F64755 |
RefSeqi | NP_414828.1, NC_000913.3 |
3D structure databases
SMRi | P71301 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259793, 6 interactors 849363, 1 interactor |
IntActi | P71301, 2 interactors |
STRINGi | 511145.b0294 |
Proteomic databases
PaxDbi | P71301 |
PRIDEi | P71301 |
Genome annotation databases
EnsemblBacteriai | AAC73397; AAC73397; b0294 BAE76078; BAE76078; BAE76078 |
GeneIDi | 944966 |
KEGGi | ecj:JW5031 eco:b0294 |
PATRICi | fig|511145.12.peg.298 |
Organism-specific databases
EchoBASEi | EB4070 |
Phylogenomic databases
eggNOGi | COG2771, Bacteria |
HOGENOMi | CLU_128111_0_0_6 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6165-MONOMER |
Miscellaneous databases
PROi | PR:P71301 |
Family and domain databases
CDDi | cd06170, LuxR_C_like, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR016032, Sig_transdc_resp-reg_C-effctor IPR000792, Tscrpt_reg_LuxR_C IPR036388, WH-like_DNA-bd_sf |
Pfami | View protein in Pfam PF00196, GerE, 1 hit |
PRINTSi | PR00038, HTHLUXR |
SMARTi | View protein in SMART SM00421, HTH_LUXR, 1 hit |
SUPFAMi | SSF46894, SSF46894, 1 hit |
PROSITEi | View protein in PROSITE PS50043, HTH_LUXR_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ECPR_ECOLI | |
Accessioni | P71301Primary (citable) accession number: P71301 Secondary accession number(s): Q2MCC8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | February 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 138 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families