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Protein

Biofilm-surface layer protein A

Gene

bslA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in biofilm formation (PubMed:18978066, PubMed:21742882). Self-polymerizes and forms a layer on the surface of biofilms that confers hydrophobicity to the biofilm (PubMed:22571672, PubMed:23904481). The layer is stable and capable of resistance to high mechanical force compression (PubMed:28701036). Required for complex colony architecture (PubMed:18978066). May function synergistically with exopolysaccharides and TasA amyloid fibers to facilitate the assembly of the biofilm matrix (PubMed:21742882).5 Publications

Enzyme and pathway databases

BioCyciBSUB:BSU31080-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Biofilm-surface layer protein A1 Publication
Alternative name(s):
ORF-1
Gene namesi
Name:bslA1 Publication
Synonyms:yuaB
Ordered Locus Names:BSU31080
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell wall, Secreted

Pathology & Biotechi

Disruption phenotypei

Overall loss in complex colony architecture and lack of fruiting body-like structures (PubMed:18978066). Disruption of the gene results in the loss of surface repellency and alters the biofilm surface microstructure (PubMed:22571672).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi76L → D: Partial loss of morphological complexity. Biofilm retains nonwetting, hydrophobic nature. 1 Publication1
Mutagenesisi76L → K: Forms flat, unwrinkled biofilm. Biofilm retains nonwetting, hydrophobic nature. 1 Publication1
Mutagenesisi77L → D or K: Forms flat, unwrinkled biofilm. Loss of colony hydrophobicity. 1 Publication1
Mutagenesisi79L → D or K: Forms flat, unwrinkled biofilm. Loss of colony hydrophobicity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000004991129 – 181Biofilm-surface layer protein ASequence analysisAdd BLAST153

Proteomic databases

PaxDbiP71014
PRIDEiP71014

Expressioni

Inductioni

Up-regulated by DegU and Spo0A. Repressed by AbrB.1 Publication

Interactioni

Subunit structurei

Forms polymers.2 Publications

Protein-protein interaction databases

IntActiP71014, 3 interactors
STRINGi224308.Bsubs1_010100016901

Structurei

Secondary structure

1181
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP71014
SMRiP71014
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the BslA/BslB family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000009071
OMAiIGSKFYA

Family and domain databases

CDDicd14670 BslA_like, 1 hit
Gene3Di2.60.40.3490, 1 hit
InterProiView protein in InterPro
IPR038480 YuaB-like_sf
IPR034650 YuaB_like
ProDomiView protein in ProDom or Entries sharing at least one domain
PD034110 PD034110, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P71014-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKRKLLSSLA ISALSLGLLV SAPTASFAAE STSTKAHTES TMRTQSTASL
60 70 80 90 100
FATITGASKT EWSFSDIELT YRPNTLLSLG VMEFTLPSGF TANTKDTLNG
110 120 130 140 150
NALRTTQILN NGKTVRVPLA LDLLGAGEFK LKLNNKTLPA AGTYTFRAEN
160 170 180
KSLSIGNKFY AEASIDVAKR STPPTQPCGC N
Length:181
Mass (Da):19,257
Last modified:July 15, 1998 - v2
Checksum:i2903AF2E5CAAFF1B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA Translation: CAB15086.1
U47861 Genomic DNA Translation: AAC44367.1
PIRiD70005
RefSeqiNP_390986.1, NC_000964.3
WP_003243556.1, NZ_JNCM01000033.1

Genome annotation databases

EnsemblBacteriaiCAB15086; CAB15086; BSU31080
GeneIDi938831
KEGGibsu:BSU31080
PATRICifig|224308.179.peg.3368

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA Translation: CAB15086.1
U47861 Genomic DNA Translation: AAC44367.1
PIRiD70005
RefSeqiNP_390986.1, NC_000964.3
WP_003243556.1, NZ_JNCM01000033.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4BHUX-ray1.91A/B/C/D/E/F/G/H/I/J48-172[»]
ProteinModelPortaliP71014
SMRiP71014
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP71014, 3 interactors
STRINGi224308.Bsubs1_010100016901

Proteomic databases

PaxDbiP71014
PRIDEiP71014

Protocols and materials databases

DNASUi938831
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15086; CAB15086; BSU31080
GeneIDi938831
KEGGibsu:BSU31080
PATRICifig|224308.179.peg.3368

Phylogenomic databases

HOGENOMiHOG000009071
OMAiIGSKFYA

Enzyme and pathway databases

BioCyciBSUB:BSU31080-MONOMER

Family and domain databases

CDDicd14670 BslA_like, 1 hit
Gene3Di2.60.40.3490, 1 hit
InterProiView protein in InterPro
IPR038480 YuaB-like_sf
IPR034650 YuaB_like
ProDomiView protein in ProDom or Entries sharing at least one domain
PD034110 PD034110, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiBSLA_BACSU
AccessioniPrimary (citable) accession number: P71014
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: September 12, 2018
This is version 99 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
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Main funding by: National Institutes of Health

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