UniProtKB - P70698 (PYRG1_MOUSE)
Protein
CTP synthase 1
Gene
Ctps1
Organism
Mus musculus (Mouse)
Status
Functioni
This enzyme is involved in the de novo synthesis of CTP, a precursor of DNA, RNA and phospholipids. Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as a source of nitrogen. This enzyme and its product, CTP, play a crucial role in the proliferation of activated lymphocytes and therefore in immunity.By similarity
Catalytic activityi
- EC:6.3.4.2By similarity
Activity regulationi
Activated by GTP and inhibited by CTP.
: CTP biosynthesis via de novo pathway Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes CTP from UDP.By similarityProteins known to be involved in the 2 steps of the subpathway in this organism are:
- no protein annotated in this organism
- CTP synthase 2 (Ctps2), CTP synthase 1 (Ctps1), CTP synthase (glutamine hydrolyzing) (Ctps2)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CTP from UDP, the pathway CTP biosynthesis via de novo pathway and in Pyrimidine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 399 | For GATase activityBy similarity | 1 | |
Active sitei | 526 | For GATase activityBy similarity | 1 | |
Active sitei | 528 | For GATase activityBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- CTP synthase activity Source: UniProtKB
- identical protein binding Source: MGI
GO - Biological processi
- 'de novo' CTP biosynthetic process Source: UniProtKB-UniPathway
- B cell proliferation Source: UniProtKB
- CTP biosynthetic process Source: UniProtKB
- glutamine metabolic process Source: UniProtKB-KW
- pyrimidine nucleobase biosynthetic process Source: GO_Central
- T cell proliferation Source: UniProtKB
Keywordsi
Molecular function | Ligase |
Biological process | Immunity, Pyrimidine biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 6.3.4.2, 3474 |
Reactomei | R-MMU-499943, Interconversion of nucleotide di- and triphosphates |
UniPathwayi | UPA00159;UER00277 |
Protein family/group databases
MEROPSi | C26.A36 |
Names & Taxonomyi
Protein namesi | Recommended name: CTP synthase 1Curated (EC:6.3.4.2By similarity)Alternative name(s): CTP synthetase 1 UTP--ammonia ligase 1 |
Gene namesi | Name:Ctps1 Synonyms:CtpsImported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1858304, Ctps |
Subcellular locationi
Cytosol
- cytosol Source: UniProtKB-SubCell
Other locations
- cytoophidium Source: MGI
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000138276 | 1 – 591 | CTP synthase 1Add BLAST | 591 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 100 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 562 | PhosphoserineBy similarity | 1 | |
Modified residuei | 568 | PhosphoserineBy similarity | 1 | |
Modified residuei | 571 | PhosphoserineBy similarity | 1 | |
Modified residuei | 573 | PhosphoserineBy similarity | 1 | |
Modified residuei | 574 | PhosphoserineCombined sources | 1 | |
Modified residuei | 575 | PhosphoserineCombined sources | 1 | |
Modified residuei | 578 | PhosphoserineCombined sources | 1 | |
Modified residuei | 587 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | P70698 |
jPOSTi | P70698 |
MaxQBi | P70698 |
PaxDbi | P70698 |
PeptideAtlasi | P70698 |
PRIDEi | P70698 |
PTM databases
iPTMneti | P70698 |
PhosphoSitePlusi | P70698 |
SwissPalmi | P70698 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000028633, Expressed in secondary oocyte and 321 other tissues |
Genevisiblei | P70698, MM |
Interactioni
GO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 206180, 67 interactors |
IntActi | P70698, 3 interactors |
MINTi | P70698 |
STRINGi | 10090.ENSMUSP00000030381 |
Miscellaneous databases
RNActi | P70698, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 300 – 554 | Glutamine amidotransferase type-1Add BLAST | 255 |
Sequence similaritiesi
Belongs to the CTP synthase family.Curated
Keywords - Domaini
Glutamine amidotransferasePhylogenomic databases
eggNOGi | KOG2387, Eukaryota |
GeneTreei | ENSGT00910000144179 |
HOGENOMi | CLU_011675_5_0_1 |
InParanoidi | P70698 |
OMAi | EFNNAYR |
OrthoDBi | 810128at2759 |
PhylomeDBi | P70698 |
TreeFami | TF300379 |
Family and domain databases
CDDi | cd01746, GATase1_CTP_Synthase, 1 hit |
Gene3Di | 3.40.50.880, 1 hit |
HAMAPi | MF_01227, PyrG, 1 hit |
InterProi | View protein in InterPro IPR029062, Class_I_gatase-like IPR004468, CTP_synthase IPR017456, CTP_synthase_N IPR017926, GATASE IPR033828, GATase1_CTP_Synthase IPR027417, P-loop_NTPase |
PANTHERi | PTHR11550, PTHR11550, 1 hit |
Pfami | View protein in Pfam PF06418, CTP_synth_N, 1 hit PF00117, GATase, 1 hit |
SUPFAMi | SSF52317, SSF52317, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00337, PyrG, 1 hit |
PROSITEi | View protein in PROSITE PS51273, GATASE_TYPE_1, 1 hit |
i Sequence
Sequence statusi: Complete.
P70698-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKYILVTGGV ISGIGKGVIA SSVGTILKSC GLHVTSIKID PYINIDAGTF
60 70 80 90 100
SPYEHGEVFV LDDGGEVDLD LGNYERFLDI RLTKDNNLTT GKIYQYVINK
110 120 130 140 150
ERKGDYLGKT VQVVPHITDA IQEWVMRQAL IPVDEDGLEP QVCVIELGGT
160 170 180 190 200
VGDIESMPFI EAFRQFQFKV KRENFCNIHV SLVPQPSSTG EQKTKPTQNS
210 220 230 240 250
VRELRGLGLS PDLVVCRCSN PLDTSVKEKI SMFCHVEPEQ VICVHDVSSI
260 270 280 290 300
YRVPLLLEEQ GVVDYFLRRL DLPIERQSRK MLMKWKEMAD RYDRLLETCS
310 320 330 340 350
IALVGKYTKF SDSYASVIKA LEHSALAINH KLEIKYIDST DLEPSTLQEE
360 370 380 390 400
PVRYHEAWQK LCSAHGVLVP GGFGVRGTEG KIQAIAWARK QKKPFLGVCL
410 420 430 440 450
GMQLAVVEFS RNVLGWQDAN STEFDPKTSH PVVIDMPEHN PGQMGGTMRL
460 470 480 490 500
GKRRTLFQTK NSVMRKLYGD TDYLEERHRH RFEVNPVLKK CLEEQGLKFV
510 520 530 540 550
GQDVEGERME IVELEDHPFF VGVQYHPEFL SRPIKPSPPY FGLLLASVGR
560 570 580 590
LPHYLQKGCR LSPRDTYSDR SGSSSPDSEI TELKFPSISQ D
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 37 | I → F in AAB06942 (Ref. 1) Curated | 1 | |
Sequence conflicti | 308 | T → P in AAB06942 (Ref. 1) Curated | 1 | |
Sequence conflicti | 388 | A → S in AAB06942 (Ref. 1) Curated | 1 | |
Sequence conflicti | 402 | M → I in AAB06942 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U49350 mRNA Translation: AAB06942.1 AK148259 mRNA Translation: BAE28443.1 BC006698 mRNA Translation: AAH06698.1 |
CCDSi | CCDS18591.1 |
RefSeqi | NP_058028.2, NM_016748.2 XP_006503288.1, XM_006503225.3 |
Genome annotation databases
Ensembli | ENSMUST00000030381; ENSMUSP00000030381; ENSMUSG00000028633 |
GeneIDi | 51797 |
KEGGi | mmu:51797 |
UCSCi | uc008unj.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U49350 mRNA Translation: AAB06942.1 AK148259 mRNA Translation: BAE28443.1 BC006698 mRNA Translation: AAH06698.1 |
CCDSi | CCDS18591.1 |
RefSeqi | NP_058028.2, NM_016748.2 XP_006503288.1, XM_006503225.3 |
3D structure databases
SMRi | P70698 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 206180, 67 interactors |
IntActi | P70698, 3 interactors |
MINTi | P70698 |
STRINGi | 10090.ENSMUSP00000030381 |
Protein family/group databases
MEROPSi | C26.A36 |
PTM databases
iPTMneti | P70698 |
PhosphoSitePlusi | P70698 |
SwissPalmi | P70698 |
Proteomic databases
EPDi | P70698 |
jPOSTi | P70698 |
MaxQBi | P70698 |
PaxDbi | P70698 |
PeptideAtlasi | P70698 |
PRIDEi | P70698 |
Protocols and materials databases
Antibodypediai | 4476, 165 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000030381; ENSMUSP00000030381; ENSMUSG00000028633 |
GeneIDi | 51797 |
KEGGi | mmu:51797 |
UCSCi | uc008unj.2, mouse |
Organism-specific databases
CTDi | 51797 |
MGIi | MGI:1858304, Ctps |
Phylogenomic databases
eggNOGi | KOG2387, Eukaryota |
GeneTreei | ENSGT00910000144179 |
HOGENOMi | CLU_011675_5_0_1 |
InParanoidi | P70698 |
OMAi | EFNNAYR |
OrthoDBi | 810128at2759 |
PhylomeDBi | P70698 |
TreeFami | TF300379 |
Enzyme and pathway databases
UniPathwayi | UPA00159;UER00277 |
BRENDAi | 6.3.4.2, 3474 |
Reactomei | R-MMU-499943, Interconversion of nucleotide di- and triphosphates |
Miscellaneous databases
BioGRID-ORCSi | 51797, 7 hits in 22 CRISPR screens |
ChiTaRSi | Ctps, mouse |
PROi | PR:P70698 |
RNActi | P70698, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000028633, Expressed in secondary oocyte and 321 other tissues |
Genevisiblei | P70698, MM |
Family and domain databases
CDDi | cd01746, GATase1_CTP_Synthase, 1 hit |
Gene3Di | 3.40.50.880, 1 hit |
HAMAPi | MF_01227, PyrG, 1 hit |
InterProi | View protein in InterPro IPR029062, Class_I_gatase-like IPR004468, CTP_synthase IPR017456, CTP_synthase_N IPR017926, GATASE IPR033828, GATase1_CTP_Synthase IPR027417, P-loop_NTPase |
PANTHERi | PTHR11550, PTHR11550, 1 hit |
Pfami | View protein in Pfam PF06418, CTP_synth_N, 1 hit PF00117, GATase, 1 hit |
SUPFAMi | SSF52317, SSF52317, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00337, PyrG, 1 hit |
PROSITEi | View protein in PROSITE PS51273, GATASE_TYPE_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PYRG1_MOUSE | |
Accessioni | P70698Primary (citable) accession number: P70698 Secondary accession number(s): Q922Y4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | July 25, 2006 | |
Last modified: | December 2, 2020 | |
This is version 157 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families