UniProtKB - P70684 (PGDH_CAVPO)
Protein
15-hydroxyprostaglandin dehydrogenase [NAD(+)]
Gene
HPGD
Organism
Cavia porcellus (Guinea pig)
Status
Functioni
Primary enzyme catalyzing the conversion of hydroxylated arachidonic acid species to their corresponding oxidized metabolites. Prostaglandin inactivation, catalyzes the first step in the catabolic pathway of the prostaglandins. Contributes to the regulation of events that are under the control of prostaglandin levels. Catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. Converts 11(R)-HETE to 11-oxo-5,8,12,14-(Z,Z,E,Z)-eicosatetraenoic acid (ETE). Has hydroxylated docosahexaenoic acid metabolites as substrates. Converts resolvins E1, D1 and D2 to their oxo products which represents a mode of resolvins inactivation and stabilizes their anti-inflammatory actions.By similarity
Catalytic activityi
- EC:1.1.1.141By similarityThis reaction proceeds in the forwardBy similarity direction.
- lipoxin A4 + NAD+ = 15-oxo-(5S,6R)-dihydroxy-(7E,9E,11Z,13E)-eicosatetraenoate + H+ + NADHBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 15-oxo-(5S,6R)-dihydroxy-(7E,9E,11Z)-eicosatrienoate + H+ + NADH = (5S,6R,15S)-trihydroxy-(7E,9E,11Z)-eicosatrienoate + NAD+By similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- (5Z,8Z,11Z,13E,15S)-hydroxyeicosatetraenoate + NAD+ = 15-oxo-(5Z,8Z,11Z,13E)-eicosatetraenoate + H+ + NADHBy similarityThis reaction proceeds in the forwardBy similarity direction.
- (11R)-hydroxy-(5Z,8Z,12E,14Z)-eicosatetraenoate + NAD+ = 11-oxo-(5Z,8Z,12E,14Z)-eicosatetraenoate + H+ + NADHBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 14-hydroxy-(4Z,7Z,10Z,12E,16Z,19Z)-docosahexaenoate + NAD+ = 14-oxo-(4Z,7Z,10Z,12E,16Z,19Z)-docosahexaenoate + H+ + NADHBy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 91 | NAD; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 138 | SubstrateBy similarity | 1 | |
Binding sitei | 148 | SubstrateBy similarity | 1 | |
Active sitei | 151 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 12 – 20 | NADBy similarity | 9 | |
Nucleotide bindingi | 36 – 37 | NADBy similarity | 2 | |
Nucleotide bindingi | 63 – 65 | NADBy similarity | 3 | |
Nucleotide bindingi | 151 – 155 | NADBy similarity | 5 | |
Nucleotide bindingi | 186 – 188 | NADBy similarity | 3 |
GO - Molecular functioni
- 15-hydroxyprostaglandin dehydrogenase (NAD+) activity Source: UniProtKB
- NAD+ binding Source: UniProtKB
- NAD binding Source: UniProtKB
- oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: UniProtKB
- prostaglandin E receptor activity Source: UniProtKB
GO - Biological processi
- ductus arteriosus closure Source: UniProtKB
- female pregnancy Source: UniProtKB
- negative regulation of cell cycle Source: UniProtKB
- ovulation Source: UniProtKB
- parturition Source: UniProtKB
- prostaglandin metabolic process Source: UniProtKB
- regulation of prostaglandin catabolic process Source: UniProtKB
- thrombin-activated receptor signaling pathway Source: UniProtKB
- transforming growth factor beta receptor signaling pathway Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Fatty acid metabolism, Lipid metabolism, Prostaglandin metabolism |
Ligand | NAD |
Names & Taxonomyi
Protein namesi | Recommended name: 15-hydroxyprostaglandin dehydrogenase [NAD(+)] (EC:1.1.1.141)Short name: 15-PGDH Alternative name(s): Prostaglandin dehydrogenase 1 |
Gene namesi | Name:HPGD Synonyms:PGDH1 |
Organismi | Cavia porcellus (Guinea pig) |
Taxonomic identifieri | 10141 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricomorpha › Caviidae › Cavia |
Proteomesi |
|
Subcellular locationi
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000253625 | 1 – 265 | 15-hydroxyprostaglandin dehydrogenase [NAD(+)]Add BLAST | 265 |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
STRINGi | 10141.ENSCPOP00000001789 |
Family & Domainsi
Sequence similaritiesi
Belongs to the short-chain dehydrogenases/reductases (SDR) family.Curated
Phylogenomic databases
eggNOGi | KOG4169, Eukaryota |
InParanoidi | P70684 |
OrthoDBi | 1053465at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
Pfami | View protein in Pfam PF00106, adh_short, 1 hit |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
i Sequence
Sequence statusi: Complete.
P70684-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MHVNGKVALV TGAAQGIGRA FAEGLLHKGA KVALVDWNLE AGVKCKAALD
60 70 80 90 100
EEFEPQKTLF IQCDVADQEQ LRDTFTKVVD YFGRLDILVN NAGVNNEKNW
110 120 130 140 150
EKTLQINLVS VISGTYLGLD YMSKQHGGEG GVIINMSSLA GLMPVAQQPV
160 170 180 190 200
YCASKHGIIG FTRSAAMARK LMNSGVRMNA ICPGFVNTSI LQSIEKEENM
210 220 230 240 250
GPYIEYTGHI KDMMKCYGIL DPEMIANGLI TLIEDDDLNG AIMKITTSNG
260
IHFQDYDTAP SYAKD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y07953 mRNA Translation: CAA69251.1 |
RefSeqi | NP_001166432.1, NM_001172961.1 |
Genome annotation databases
GeneIDi | 100135538 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y07953 mRNA Translation: CAA69251.1 |
RefSeqi | NP_001166432.1, NM_001172961.1 |
3D structure databases
SMRi | P70684 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10141.ENSCPOP00000001789 |
Genome annotation databases
GeneIDi | 100135538 |
Organism-specific databases
CTDi | 3248 |
Phylogenomic databases
eggNOGi | KOG4169, Eukaryota |
InParanoidi | P70684 |
OrthoDBi | 1053465at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
Pfami | View protein in Pfam PF00106, adh_short, 1 hit |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PGDH_CAVPO | |
Accessioni | P70684Primary (citable) accession number: P70684 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 17, 2006 |
Last sequence update: | February 1, 1997 | |
Last modified: | August 12, 2020 | |
This is version 107 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families