UniProtKB - P70673 (KCJ11_RAT)
Protein
ATP-sensitive inward rectifier potassium channel 11
Gene
Kcnj11
Organism
Rattus norvegicus (Rat)
Status
Functioni
This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium. Can form cardiac and smooth muscle-type KATP channels with ABCC9. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 160 | Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity | 1 |
GO - Molecular functioni
- ankyrin binding Source: BHF-UCL
- ATP-activated inward rectifier potassium channel activity Source: RGD
- ATPase-coupled cation transmembrane transporter activity Source: ARUK-UCL
- ATP binding Source: RGD
- heat shock protein binding Source: RGD
- inward rectifier potassium channel activity Source: GO_Central
- ion channel binding Source: RGD
- protein C-terminus binding Source: RGD
- voltage-gated potassium channel activity Source: RGD
GO - Biological processi
- cellular response to glucose stimulus Source: RGD
- cellular response to nicotine Source: RGD
- cellular response to tumor necrosis factor Source: RGD
- glucose metabolic process Source: RGD
- inorganic cation transmembrane transport Source: ARUK-UCL
- negative regulation of insulin secretion Source: RGD
- nervous system process Source: RGD
- positive regulation of cation channel activity Source: RGD
- positive regulation of protein localization to plasma membrane Source: RGD
- potassium ion import across plasma membrane Source: RGD
- potassium ion transmembrane transport Source: RGD
- potassium ion transport Source: RGD
- regulation of insulin secretion Source: RGD
- regulation of ion transmembrane transport Source: GO_Central
- regulation of membrane potential Source: RGD
- response to ATP Source: ARUK-UCL
- response to drug Source: RGD
- response to estradiol Source: RGD
- response to ischemia Source: RGD
- response to testosterone Source: RGD
Keywordsi
Molecular function | Ion channel, Voltage-gated channel |
Biological process | Ion transport, Potassium transport, Transport |
Ligand | Potassium |
Enzyme and pathway databases
Reactomei | R-RNO-1296025, ATP sensitive Potassium channels R-RNO-382556, ABC-family proteins mediated transport R-RNO-422356, Regulation of insulin secretion R-RNO-5578775, Ion homeostasis |
Protein family/group databases
TCDBi | 1.A.2.1.7, the inward rectifier k(+) channel (irk-c) family |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-sensitive inward rectifier potassium channel 11Alternative name(s): BIR Inward rectifier K(+) channel Kir6.2 Potassium channel, inwardly rectifying subfamily J member 11 |
Gene namesi | Name:Kcnj11 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 69247, Kcnj11 |
Subcellular locationi
Other locations
Cytosol
- cytosol Source: RGD
Endoplasmic reticulum
- endoplasmic reticulum Source: RGD
Endosome
- endosome Source: RGD
Mitochondrion
- mitochondrion Source: RGD
Nucleus
- nuclear envelope Source: RGD
Plasma Membrane
- axolemma Source: RGD
- inward rectifying potassium channel Source: RGD
- plasma membrane Source: RGD
- sarcolemma Source: RGD
- T-tubule Source: RGD
Other locations
- acrosomal vesicle Source: RGD
- cell body fiber Source: RGD
- intercalated disc Source: RGD
- intracellular membrane-bounded organelle Source: RGD
- myelin sheath Source: RGD
- neuronal cell body Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 68 | CytoplasmicBy similarityAdd BLAST | 68 | |
Transmembranei | 69 – 93 | Helical; Name=M1By similarityAdd BLAST | 25 | |
Topological domaini | 94 – 116 | ExtracellularBy similarityAdd BLAST | 23 | |
Intramembranei | 117 – 128 | Helical; Pore-forming; Name=H5By similarityAdd BLAST | 12 | |
Intramembranei | 129 – 135 | Pore-formingBy similarity | 7 | |
Topological domaini | 136 – 144 | ExtracellularBy similarity | 9 | |
Transmembranei | 145 – 166 | Helical; Name=M2By similarityAdd BLAST | 22 | |
Topological domaini | 167 – 390 | CytoplasmicBy similarityAdd BLAST | 224 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000154960 | 1 – 390 | ATP-sensitive inward rectifier potassium channel 11Add BLAST | 390 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 341 | Phosphothreonine; by MAPK1By similarity | 1 | |
Modified residuei | 385 | Phosphoserine; by MAPK1By similarity | 1 |
Post-translational modificationi
Phosphorylation by MAPK1 results in changes in channel gating that destabilize the closed states and reduce the ATP sensitivity.By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P70673 |
PRIDEi | P70673 |
PTM databases
iPTMneti | P70673 |
PhosphoSitePlusi | P70673 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000021128, Expressed in skeletal muscle tissue and 20 other tissues |
Genevisiblei | P70673, RN |
Interactioni
Subunit structurei
Interacts with ABCC9/SUR2 (By similarity).
Interacts with ABCC8/SUR.
By similarityGO - Molecular functioni
- ankyrin binding Source: BHF-UCL
- heat shock protein binding Source: RGD
- ion channel binding Source: RGD
- protein C-terminus binding Source: RGD
Protein-protein interaction databases
ComplexPortali | CPX-181, Inward rectifying potassium channel complex, Kir6.2-SUR2A CPX-185, Inward rectifying potassium channel complex, Kir6.2-SUR2B CPX-186, Inward rectifying potassium channel complex, Kir6.2-SUR1 |
CORUMi | P70673 |
DIPi | DIP-58641N |
IntActi | P70673, 2 interactors |
STRINGi | 10116.ENSRNOP00000028685 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 130 – 135 | Selectivity filterBy similarity | 6 |
Sequence similaritiesi
Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ11 subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3827, Eukaryota |
GeneTreei | ENSGT01020000230334 |
HOGENOMi | CLU_022738_4_0_1 |
InParanoidi | P70673 |
OMAi | DNPVGTN |
OrthoDBi | 956263at2759 |
PhylomeDBi | P70673 |
TreeFami | TF313676 |
Family and domain databases
Gene3Di | 2.60.40.1400, 1 hit |
InterProi | View protein in InterPro IPR014756, Ig_E-set IPR041647, IRK_C IPR016449, K_chnl_inward-rec_Kir IPR003279, K_chnl_inward-rec_Kir6.2 IPR013518, K_chnl_inward-rec_Kir_cyto IPR040445, Kir_TM |
PANTHERi | PTHR11767, PTHR11767, 1 hit PTHR11767:SF44, PTHR11767:SF44, 1 hit |
Pfami | View protein in Pfam PF01007, IRK, 1 hit PF17655, IRK_C, 1 hit |
PIRSFi | PIRSF005465, GIRK_kir, 1 hit |
PRINTSi | PR01332, KIR62CHANNEL PR01320, KIRCHANNEL |
SUPFAMi | SSF81296, SSF81296, 1 hit |
i Sequence
Sequence statusi: Complete.
P70673-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLSRKGIIPE EYVLTRLAED PTEPRYRTRE RRARFVSKKG NCNVAHKNIR
60 70 80 90 100
EQGRFLQDVF TTLVDLKWPH TLLIFTMSFL CSWLLFAMVW WLIAFAHGDL
110 120 130 140 150
APGEGTNVPC VTSIHSFSSA FLFSIEVQVT IGFGGRMVTE ECPLAILILI
160 170 180 190 200
VQNIVGLMIN AIMLGCIFMK TAQAHRRAET LIFSKHAVIT LRHGRLCFML
210 220 230 240 250
RVGDLRKSMI ISATIHMQVV RKTTSPEGEV VPLHQVDIPM ENGVGGNSIF
260 270 280 290 300
LVAPLIIYHV IDSNSPLYDL APSDLHHHQD LEIIVILEGV VETTGITTQA
310 320 330 340 350
RTSYLADEIL WGQRFVPIVA EEDGRYSVDY SKFGNTVKVP TPLCTARQLD
360 370 380 390
EDRSLLDALT LASSRGPLRK RSVAVAKAKP KFSISPDSLS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 191 | L → P in BAA96239 (Ref. 5) Curated | 1 | |
Sequence conflicti | 365 | R → Q in AAB01810 (PubMed:8607800).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D86039 mRNA Translation: BAA12971.1 X97041 mRNA Translation: CAA65754.1 U44897 mRNA Translation: AAB01810.1 D61687 mRNA Translation: BAA23630.1 AB043638 mRNA Translation: BAA96239.1 |
PIRi | JC4689 |
RefSeqi | NP_112648.2, NM_031358.3 |
Genome annotation databases
Ensembli | ENSRNOT00000028685; ENSRNOP00000028685; ENSRNOG00000021128 |
GeneIDi | 83535 |
KEGGi | rno:83535 |
UCSCi | RGD:69247, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D86039 mRNA Translation: BAA12971.1 X97041 mRNA Translation: CAA65754.1 U44897 mRNA Translation: AAB01810.1 D61687 mRNA Translation: BAA23630.1 AB043638 mRNA Translation: BAA96239.1 |
PIRi | JC4689 |
RefSeqi | NP_112648.2, NM_031358.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5TWV | electron microscopy | 6.30 | A/C/E/G | 1-390 | [»] | |
6BAA | electron microscopy | 3.63 | A/B/C/D | 1-390 | [»] | |
6PZ9 | electron microscopy | 3.65 | D | 1-390 | [»] | |
6PZA | electron microscopy | 3.74 | D | 1-390 | [»] | |
SMRi | P70673 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
ComplexPortali | CPX-181, Inward rectifying potassium channel complex, Kir6.2-SUR2A CPX-185, Inward rectifying potassium channel complex, Kir6.2-SUR2B CPX-186, Inward rectifying potassium channel complex, Kir6.2-SUR1 |
CORUMi | P70673 |
DIPi | DIP-58641N |
IntActi | P70673, 2 interactors |
STRINGi | 10116.ENSRNOP00000028685 |
Chemistry databases
ChEMBLi | CHEMBL3038487 |
Protein family/group databases
TCDBi | 1.A.2.1.7, the inward rectifier k(+) channel (irk-c) family |
PTM databases
iPTMneti | P70673 |
PhosphoSitePlusi | P70673 |
Proteomic databases
PaxDbi | P70673 |
PRIDEi | P70673 |
Protocols and materials databases
ABCDi | P70673, 1 sequenced antibody |
Genome annotation databases
Ensembli | ENSRNOT00000028685; ENSRNOP00000028685; ENSRNOG00000021128 |
GeneIDi | 83535 |
KEGGi | rno:83535 |
UCSCi | RGD:69247, rat |
Organism-specific databases
CTDi | 3767 |
RGDi | 69247, Kcnj11 |
Phylogenomic databases
eggNOGi | KOG3827, Eukaryota |
GeneTreei | ENSGT01020000230334 |
HOGENOMi | CLU_022738_4_0_1 |
InParanoidi | P70673 |
OMAi | DNPVGTN |
OrthoDBi | 956263at2759 |
PhylomeDBi | P70673 |
TreeFami | TF313676 |
Enzyme and pathway databases
Reactomei | R-RNO-1296025, ATP sensitive Potassium channels R-RNO-382556, ABC-family proteins mediated transport R-RNO-422356, Regulation of insulin secretion R-RNO-5578775, Ion homeostasis |
Miscellaneous databases
PROi | PR:P70673 |
Gene expression databases
Bgeei | ENSRNOG00000021128, Expressed in skeletal muscle tissue and 20 other tissues |
Genevisiblei | P70673, RN |
Family and domain databases
Gene3Di | 2.60.40.1400, 1 hit |
InterProi | View protein in InterPro IPR014756, Ig_E-set IPR041647, IRK_C IPR016449, K_chnl_inward-rec_Kir IPR003279, K_chnl_inward-rec_Kir6.2 IPR013518, K_chnl_inward-rec_Kir_cyto IPR040445, Kir_TM |
PANTHERi | PTHR11767, PTHR11767, 1 hit PTHR11767:SF44, PTHR11767:SF44, 1 hit |
Pfami | View protein in Pfam PF01007, IRK, 1 hit PF17655, IRK_C, 1 hit |
PIRSFi | PIRSF005465, GIRK_kir, 1 hit |
PRINTSi | PR01332, KIR62CHANNEL PR01320, KIRCHANNEL |
SUPFAMi | SSF81296, SSF81296, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KCJ11_RAT | |
Accessioni | P70673Primary (citable) accession number: P70673 Secondary accession number(s): Q62906, Q9JM48 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 163 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families