UniProtKB - P70615 (LMNB1_RAT)
Protein
Lamin-B1
Gene
Lmnb1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.By similarity
Miscellaneous
The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively.By similarity
GO - Molecular functioni
- double-stranded DNA binding Source: RGD
- phospholipase binding Source: RGD
- sequence-specific double-stranded DNA binding Source: RGD
GO - Biological processi
- apoptotic process Source: RGD
- cellular response to corticosterone stimulus Source: RGD
- cellular response to monosodium L-glutamate Source: RGD
- cellular response to organic cyclic compound Source: RGD
- neurogenesis Source: RGD
- response to antibiotic Source: RGD
- response to drug Source: RGD
Enzyme and pathway databases
Reactomei | R-RNO-2559584, Formation of Senescence-Associated Heterochromatin Foci (SAHF) R-RNO-2980766, Nuclear Envelope Breakdown R-RNO-2995383, Initiation of Nuclear Envelope (NE) Reformation R-RNO-352238, Breakdown of the nuclear lamina R-RNO-4419969, Depolymerisation of the Nuclear Lamina |
Names & Taxonomyi
Protein namesi | Recommended name: Lamin-B1 |
Gene namesi | Name:Lmnb1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620522, Lmnb1 |
Subcellular locationi
Nucleus
- Nucleus inner membrane By similarity; Lipid-anchor By similarity; Nucleoplasmic side By similarity
Cytoskeleton
- lamin filament Source: RGD
Nucleus
- lamin filament Source: RGD
- nuclear envelope Source: RGD
- nuclear inner membrane Source: RGD
- nuclear lamina Source: RGD
- nuclear matrix Source: RGD
- nuclear membrane Source: RGD
- nucleoplasm Source: RGD
- nucleus Source: RGD
Other locations
- cytoplasm Source: RGD
Keywords - Cellular componenti
Intermediate filament, Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000063818 | 2 – 584 | Lamin-B1Add BLAST | 583 | |
PropeptideiPRO_0000403468 | 585 – 587 | Removed in mature formBy similarity | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 3 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 5 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 14 | Omega-N-methylarginineBy similarity | 1 | |
Modified residuei | 16 | PhosphoserineBy similarity | 1 | |
Modified residuei | 20 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 23 | PhosphoserineBy similarity | 1 | |
Modified residuei | 25 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 28 | PhosphoserineBy similarity | 1 | |
Cross-linki | 102 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 111 | N6-acetyllysineBy similarity | 1 | |
Cross-linki | 123 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 126 | PhosphoserineCombined sources | 1 | |
Cross-linki | 145 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 157 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 157 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 158 | PhosphoserineCombined sources | 1 | |
Cross-linki | 181 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 200 | PhosphoserineBy similarity | 1 | |
Modified residuei | 232 | PhosphoserineBy similarity | 1 | |
Cross-linki | 241 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 261 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 271 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 271 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 278 | PhosphoserineBy similarity | 1 | |
Modified residuei | 302 | PhosphoserineBy similarity | 1 | |
Cross-linki | 312 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Disulfide bondi | 317 | InterchainBy similarity | ||
Modified residuei | 330 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 330 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 375 | PhosphoserineBy similarity | 1 | |
Modified residuei | 413 | Omega-N-methylarginineBy similarity | 1 | |
Modified residuei | 483 | N6-acetyllysineBy similarity | 1 | |
Cross-linki | 532 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 534 | PhosphoserineBy similarity | 1 | |
Cross-linki | 547 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 576 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 584 | Cysteine methyl esterBy similarity | 1 | |
Lipidationi | 584 | S-farnesyl cysteineBy similarity | 1 |
Post-translational modificationi
B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations (By similarity).By similarity
Keywords - PTMi
Acetylation, Disulfide bond, Isopeptide bond, Lipoprotein, Methylation, Phosphoprotein, Prenylation, Ubl conjugationProteomic databases
jPOSTi | P70615 |
PaxDbi | P70615 |
PRIDEi | P70615 |
2D gel databases
World-2DPAGEi | 0004:P70615 |
PTM databases
CarbonylDBi | P70615 |
iPTMneti | P70615 |
PhosphoSitePlusi | P70615 |
Interactioni
Subunit structurei
Homodimer.
Interacts with lamin-associated polypeptides IA, IB and 2.
Interacts with SPAG4 and SEPT12 (By similarity).
By similarityGO - Molecular functioni
- phospholipase binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 250568, 13 interactors |
STRINGi | 10116.ENSRNOP00000019351 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 32 – 388 | IF rodPROSITE-ProRule annotationAdd BLAST | 357 | |
Domaini | 430 – 546 | LTDPROSITE-ProRule annotationAdd BLAST | 117 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 34 | HeadAdd BLAST | 33 | |
Regioni | 35 – 69 | Coil 1AAdd BLAST | 35 | |
Regioni | 70 – 81 | Linker 1Add BLAST | 12 | |
Regioni | 82 – 215 | Coil 1BAdd BLAST | 134 | |
Regioni | 216 – 243 | Linker 2Add BLAST | 28 | |
Regioni | 244 – 386 | Coil 2Add BLAST | 143 | |
Regioni | 387 – 587 | TailAdd BLAST | 201 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 415 – 420 | Nuclear localization signalSequence analysis | 6 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 552 – 560 | Glu-rich (highly acidic; could be involved in chromatin binding) | 9 |
Sequence similaritiesi
Belongs to the intermediate filament family.PROSITE-ProRule annotation
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0977, Eukaryota |
InParanoidi | P70615 |
OrthoDBi | 701388at2759 |
PhylomeDBi | P70615 |
Family and domain databases
Gene3Di | 1.20.5.1160, 2 hits 2.60.40.1260, 1 hit |
InterProi | View protein in InterPro IPR018039, IF_conserved IPR039008, IF_rod_dom IPR042180, IF_rod_dom_coil1B IPR001322, Lamin_tail_dom IPR036415, Lamin_tail_dom_sf |
Pfami | View protein in Pfam PF00038, Filament, 1 hit PF00932, LTD, 1 hit |
SMARTi | View protein in SMART SM01391, Filament, 1 hit |
SUPFAMi | SSF74853, SSF74853, 1 hit |
PROSITEi | View protein in PROSITE PS00226, IF_ROD_1, 1 hit PS51842, IF_ROD_2, 1 hit PS51841, LTD, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P70615-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATATPVQQR AGSRASAPAT PFSPTRLSRL QEKEELRELN DRLAVYIDKV
60 70 80 90 100
RSLETENSAL QLQVTEREEV RGRELTGLKA LYETELADAR RALDDTARER
110 120 130 140 150
AKLQIELGKF KAEHDQLLLN YAKKESDLSG AQIKLREYEA ALNSKDAALA
160 170 180 190 200
TALGDKKSLE GDLEDLKDQI AQLEASLSAA KKQLADETLL KVDLENRCQS
210 220 230 240 250
LTEDLEFRKN MYEEEINETR RKHETRLVEV DSGRQIEYEY KLAQALHEMR
260 270 280 290 300
EQHDAQVRLY KEELEQTYHA KLENARLSSE MNTSTVNSAR GGMMESRMRI
310 320 330 340 350
ESLSSQLSNL QKDSRACLER IQELEDMLAK ERDNSRRMLS DKEREMAEIR
360 370 380 390 400
DQMQQQLNDY EQLLDVKLAL DMEISAYRKL LEGEEERLKL SPSPSSRVTV
410 420 430 440 450
SRASSSRSVR TTRGKRKRVD VEESEASSSV SISHSASATG NVCIEEIDVD
460 470 480 490 500
GKFIRLKNTS EQDQPMGGWE MIRKIGDTSV SYKYTSRYVL KAGQTVTVWA
510 520 530 540 550
ANAGVTASPP TDLIWKNQNS WGTGEDVKVV LKNSQGEEVA QRSTVFKTTI
560 570 580
PEEEEEEEEE PIGVPLEEER FHQQGTPRAS NKSCAIM
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG3V7U4 | G3V7U4_RAT | Lamin-B1 | Lmnb1 rCG_46767 | 587 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U72353 mRNA Translation: AAB09600.1 |
RefSeqi | NP_446357.1, NM_053905.1 |
Genome annotation databases
GeneIDi | 116685 |
KEGGi | rno:116685 |
UCSCi | RGD:620522, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U72353 mRNA Translation: AAB09600.1 |
RefSeqi | NP_446357.1, NM_053905.1 |
3D structure databases
BMRBi | P70615 |
SMRi | P70615 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 250568, 13 interactors |
STRINGi | 10116.ENSRNOP00000019351 |
PTM databases
CarbonylDBi | P70615 |
iPTMneti | P70615 |
PhosphoSitePlusi | P70615 |
2D gel databases
World-2DPAGEi | 0004:P70615 |
Proteomic databases
jPOSTi | P70615 |
PaxDbi | P70615 |
PRIDEi | P70615 |
Genome annotation databases
GeneIDi | 116685 |
KEGGi | rno:116685 |
UCSCi | RGD:620522, rat |
Organism-specific databases
CTDi | 4001 |
RGDi | 620522, Lmnb1 |
Phylogenomic databases
eggNOGi | KOG0977, Eukaryota |
InParanoidi | P70615 |
OrthoDBi | 701388at2759 |
PhylomeDBi | P70615 |
Enzyme and pathway databases
Reactomei | R-RNO-2559584, Formation of Senescence-Associated Heterochromatin Foci (SAHF) R-RNO-2980766, Nuclear Envelope Breakdown R-RNO-2995383, Initiation of Nuclear Envelope (NE) Reformation R-RNO-352238, Breakdown of the nuclear lamina R-RNO-4419969, Depolymerisation of the Nuclear Lamina |
Miscellaneous databases
PROi | PR:P70615 |
Family and domain databases
Gene3Di | 1.20.5.1160, 2 hits 2.60.40.1260, 1 hit |
InterProi | View protein in InterPro IPR018039, IF_conserved IPR039008, IF_rod_dom IPR042180, IF_rod_dom_coil1B IPR001322, Lamin_tail_dom IPR036415, Lamin_tail_dom_sf |
Pfami | View protein in Pfam PF00038, Filament, 1 hit PF00932, LTD, 1 hit |
SMARTi | View protein in SMART SM01391, Filament, 1 hit |
SUPFAMi | SSF74853, SSF74853, 1 hit |
PROSITEi | View protein in PROSITE PS00226, IF_ROD_1, 1 hit PS51842, IF_ROD_2, 1 hit PS51841, LTD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LMNB1_RAT | |
Accessioni | P70615Primary (citable) accession number: P70615 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 13, 2001 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 146 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families