Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Hepatocyte nuclear factor 6

Gene

Onecut1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription. The affinity of HNF-6-alpha and HNF-6-beta for DNA differs depending on the target sequence.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi283 – 369CUTPROSITE-ProRule annotationAdd BLAST87
DNA bindingi385 – 444HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

  • DNA binding Source: RGD
  • transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: BHF-UCL
  • transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding Source: RGD

GO - Biological processi

  • cell differentiation Source: GO_Central
  • glucose metabolic process Source: RGD
  • positive regulation of transcription by RNA polymerase II Source: BHF-UCL
  • system development Source: GO_Central

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Hepatocyte nuclear factor 6
Short name:
HNF-6
Alternative name(s):
One cut domain family member 1
One cut homeobox 1
Gene namesi
Name:Onecut1
Synonyms:Hnf6, Hnf6a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2811 Onecut1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002024041 – 465Hepatocyte nuclear factor 6Add BLAST465

Proteomic databases

PRIDEiP70512

Expressioni

Tissue specificityi

Expressed in liver, brain, spleen and testis.

Interactioni

Subunit structurei

Binds DNA as a monomer.

Structurei

Secondary structure

1465
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi294 – 307Combined sources14
Helixi312 – 318Combined sources7
Helixi324 – 332Combined sources9
Helixi337 – 339Combined sources3
Helixi344 – 355Combined sources12
Helixi358 – 364Combined sources7
Helixi394 – 406Combined sources13
Helixi412 – 422Combined sources11
Helixi426 – 438Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D5VX-ray2.00A/B289-444[»]
ProteinModelPortaliP70512
SMRiP70512
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP70512

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi69 – 72Poly-His4
Compositional biasi124 – 138Poly-HisAdd BLAST15
Compositional biasi455 – 460Poly-Ser6

Sequence similaritiesi

Belongs to the CUT homeobox family.Curated

Keywords - Domaini

Homeobox

Phylogenomic databases

HOGENOMiHOG000232114
HOVERGENiHBG005981
InParanoidiP70512
KOiK08026

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR003350 CUT_dom
IPR009057 Homeobox-like_sf
IPR001356 Homeobox_dom
IPR010982 Lambda_DNA-bd_dom_sf
PfamiView protein in Pfam
PF02376 CUT, 1 hit
PF00046 Homeobox, 1 hit
SMARTiView protein in SMART
SM01109 CUT, 1 hit
SM00389 HOX, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
SSF47413 SSF47413, 1 hit
PROSITEiView protein in PROSITE
PS51042 CUT, 1 hit
PS50071 HOMEOBOX_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha (identifier: P70512-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNAQLTMEAI GELHGVSHEP VPAPADLLGG SPHARSSVGH RGSHLPPAHP
60 70 80 90 100
RSMGMASLLD GGSGGSDYHH HHRAPEHSLA GPLHPTMTMA CETPPGMSMP
110 120 130 140 150
TTYTTLTPLQ PLPPISTVSD KFPHHHHHHH HHHHPHHHQR LAGNVSGSFT
160 170 180 190 200
LMRDERGLAS MNNLYTPYHK DVAGMGQSLS PLSGSGLGSI HNSQQGLPHY
210 220 230 240 250
AHPGAAMPTD KMLTPNDFEA HHPAMLGRHG EQHLTPTSAG MVPINGLPPH
260 270 280 290 300
HPHAHLNAQG HGQLLGTARE PNPSVTGAQV SNGSNSGQME EINTKEVAQR
310 320 330 340 350
ITTELKRYSI PQAIFAQRVL CRSQGTLSDL LRNPKPWSKL KSGRETFRRM
360 370 380 390 400
WKWLQEPEFQ RMSALRLAAC KRKEQEHGKD RGNTPKKPRL VFTDVQRRTL
410 420 430 440 450
HAIFKENKRP SKELQITISQ QLGLELSTVS NFFMNARRRS LDKWQDEGSS
460
NSGNSSSSSS TCTKA
Length:465
Mass (Da):51,067
Last modified:February 1, 1997 - v1
Checksum:iBD651267FD7AC896
GO
Isoform Beta (identifier: P70512-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     368-368: A → AESAMGGSVPSLRITSGGPQLSVPPLP

Show »
Length:491
Mass (Da):53,584
Checksum:iC0B87B4FB6F6C978
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002312368A → AESAMGGSVPSLRITSGGPQ LSVPPLP in isoform Beta. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96553 Genomic DNA Translation: CAA65389.1
Y14933 mRNA Translation: CAA75150.1
RefSeqiNP_073162.1, NM_022671.2 [P70512-1]
UniGeneiRn.48812

Genome annotation databases

GeneIDi25231
KEGGirno:25231
UCSCiRGD:2811 rat [P70512-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiHNF6_RAT
AccessioniPrimary (citable) accession number: P70512
Secondary accession number(s): O88755
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: February 1, 1997
Last modified: May 23, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health