UniProtKB - P70460 (VASP_MOUSE)
Vasodilator-stimulated phosphoprotein
Vasp
Functioni
GO - Molecular functioni
- actin binding Source: UniProtKB-KW
- profilin binding Source: MGI
- SH3 domain binding Source: GO_Central
GO - Biological processi
- actin cytoskeleton organization Source: MGI
- actin polymerization or depolymerization Source: InterPro
- axon guidance Source: MGI
- neural tube closure Source: MGI
- positive regulation of actin filament polymerization Source: MGI
- protein homotetramerization Source: InterPro
Keywordsi
Molecular function | Actin-binding |
Enzyme and pathway databases
Reactomei | R-MMU-376176, Signaling by ROBO receptors R-MMU-446353, Cell-extracellular matrix interactions |
Names & Taxonomyi
Protein namesi | Recommended name: Vasodilator-stimulated phosphoproteinShort name: VASP |
Gene namesi | Name:Vasp |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:109268, Vasp |
Subcellular locationi
Cytoskeleton
- cytoskeleton 1 Publication
Plasma membrane
- lamellipodium membrane 1 Publication
- filopodium membrane 1 Publication
Other locations
- Cytoplasm 1 Publication
- focal adhesion 1 Publication
- tight junction By similarity
Note: Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions. Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions (By similarity). In pre-apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes) (By similarity).By similarity
Cytoskeleton
- cytoskeleton Source: UniProtKB-SubCell
Cytosol
- cytosol Source: MGI
Plasma Membrane
- filopodium membrane Source: UniProtKB-SubCell
- lamellipodium membrane Source: UniProtKB-SubCell
- plasma membrane Source: MGI
Other locations
- bicellular tight junction Source: UniProtKB-SubCell
- filopodium Source: MGI
- focal adhesion Source: MGI
- lamellipodium Source: MGI
Keywords - Cellular componenti
Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Tight junctionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 153 | S → D: Reduces actin polymerization to a lesser extent than wild-type. Greater effect on actin polymerization; when associated with D-235 and E-274. 1 Publication | 1 | |
Mutagenesisi | 232 – 233 | RK → GE or EE: Reduced binding to monomeric actin. No VASP-induced actin polymerization. 1 Publication | 2 | |
Mutagenesisi | 235 | S → D: Reduces actin polymerization to a lesser extent than wild-type. 1 Publication | 1 | |
Mutagenesisi | 274 | T → E: Reduces actin polymerization to a lesser extent than wild-type. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000065768 | 2 – 375 | Vasodilator-stimulated phosphoproteinAdd BLAST | 374 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 39 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 46 | PhosphoserineBy similarity | 1 | |
Modified residuei | 153 | Phosphoserine; by PKA, PKC, PKG/PRKG1 and ROCK11 Publication | 1 | |
Modified residuei | 235 | Phosphoserine; by PKA and PKGCombined sources1 Publication | 1 | |
Modified residuei | 274 | Phosphothreonine; by PKA, PKG/PRKG1 and AMPKBy similarity | 1 | |
Modified residuei | 279 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 309 | PhosphoserineBy similarity | 1 | |
Modified residuei | 311 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 317 | PhosphoserineCombined sources | 1 | |
Modified residuei | 318 | Phosphoserine; by AMPK1 Publication | 1 | |
Modified residuei | 320 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | P70460 |
jPOSTi | P70460 |
MaxQBi | P70460 |
PaxDbi | P70460 |
PeptideAtlasi | P70460 |
PRIDEi | P70460 |
PTM databases
iPTMneti | P70460 |
PhosphoSitePlusi | P70460 |
SwissPalmi | P70460 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000030403, Expressed in granulocyte and 291 other tissues |
Genevisiblei | P70460, MM |
Interactioni
Subunit structurei
Homotetramer (By similarity).
Interacts with PFN1, PFN2, LPP, ACTN1 and ACTG1.
Interacts, via the EVH1 domain, with the Pro-rich regions of ZYX. This interaction is important for targeting to focal adhesions and the formation of actin-rich structures at the apical surface of cells.
Interacts, via the EVH1 domain, with the Pro-rich domain of Listeria monocytogenes actA.
Interacts with APBB1IP.
Interacts, via the Pro-rich domain, with the C-terminal SH3 domain of DNMBP.
Interacts weakly with MEFV (By similarity).
By similarityGO - Molecular functioni
- actin binding Source: UniProtKB-KW
- profilin binding Source: MGI
- SH3 domain binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 204499, 6 interactors |
DIPi | DIP-29360N |
IntActi | P70460, 5 interactors |
MINTi | P70460 |
STRINGi | 10090.ENSMUSP00000032561 |
Miscellaneous databases
RNActi | P70460, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 113 | WH1PROSITE-ProRule annotationAdd BLAST | 112 | |
Repeati | 340 – 354 | 1Add BLAST | 15 | |
Repeati | 355 – 369 | 2Add BLAST | 15 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 221 – 373 | EVH2Add BLAST | 153 | |
Regioni | 221 – 241 | EVH2 block AAdd BLAST | 21 | |
Regioni | 257 – 274 | EVH2 block BAdd BLAST | 18 | |
Regioni | 338 – 372 | EVH2 block CAdd BLAST | 35 | |
Regioni | 339 – 368 | 2 X 15 AA tandem repeats of L-[EQ]-[KR] [MV]-K-[EQ]-E-[IL]-[IL]-E-[AEV]-[FV]-[KRV]-[KQ]-EAdd BLAST | 30 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 337 – 367 | Sequence analysisAdd BLAST | 31 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 230 – 233 | KLKR | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 166 – 182 | Poly-ProAdd BLAST | 17 | |
Compositional biasi | 317 – 320 | Poly-Ser | 4 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coil, Repeat, SH3-bindingPhylogenomic databases
eggNOGi | KOG4590, Eukaryota |
GeneTreei | ENSGT00940000156765 |
HOGENOMi | CLU_017790_0_0_1 |
InParanoidi | P70460 |
OMAi | IRGVKYN |
OrthoDBi | 972128at2759 |
PhylomeDBi | P70460 |
TreeFami | TF321411 |
Family and domain databases
Gene3Di | 1.20.5.1160, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR042180, IF_rod_dom_coil1B IPR011993, PH-like_dom_sf IPR034367, VASP IPR017354, VASP/EVL IPR038023, VASP_sf IPR014885, VASP_tetra IPR000697, WH1/EVH1_dom |
PANTHERi | PTHR11202:SF12, PTHR11202:SF12, 1 hit |
Pfami | View protein in Pfam PF08776, VASP_tetra, 1 hit PF00568, WH1, 1 hit |
PIRSFi | PIRSF038010, Vasodilator_Phospo, 1 hit |
SMARTi | View protein in SMART SM00461, WH1, 1 hit |
SUPFAMi | SSF118370, SSF118370, 1 hit |
PROSITEi | View protein in PROSITE PS50229, WH1, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSETVICSSR ATVMLYDDSN KRWLPAGTGP QAFSRVQIYH NPTANSFRVV
60 70 80 90 100
GRKMQPDQQV VINCAIIRGV KYNQATPIFH QWRDARQVWG LNFGSKEDAI
110 120 130 140 150
QFATGMANAL EALEGGGPPP APAPPAWSAQ NGPSPEELEQ QKRQPEHMER
160 170 180 190 200
RVSNAGGPPA PPAGGPPPPP GPPPPPGPPP PPGLPSSGVS GAGHGAGAAP
210 220 230 240 250
PPAPPLPTAQ GPNSGGSGAP GLAAAIAGAK LRKVSKQEEA SGGPLAPKAE
260 270 280 290 300
NSRSTGGGLM EEMNAMLARR RKATQVGEKP PKDESASEES EARLPAQSEP
310 320 330 340 350
VRRPWEKNST TLPRMKSSSS VTTSEAHPST PCSSDDSDLE RVKQELLEEV
360 370
RKELQKMKEE IIEVFVQELR KRGSP
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 106 – 115 | Missing in BAE42025 (PubMed:16141072).Curated | 10 | |
Sequence conflicti | 237 | Missing in BAE33933 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 337 | S → Y in BAE29310 (PubMed:16141072).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 209 | A → T2 Publications | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X98475 Genomic DNA Translation: CAA67108.1 Sequence problems. AF084548 mRNA Translation: AAD16045.1 AK140329 mRNA Translation: BAE24338.1 AK150103 mRNA Translation: BAE29310.1 AK157021 mRNA Translation: BAE33933.1 AK170780 mRNA Translation: BAE42025.1 AK171715 mRNA Translation: BAE42628.1 BC010223 mRNA Translation: AAH10223.1 BC015289 mRNA Translation: AAH15289.1 |
CCDSi | CCDS52056.1 |
RefSeqi | NP_001268950.1, NM_001282021.1 NP_001268951.1, NM_001282022.1 NP_033525.2, NM_009499.3 |
Genome annotation databases
Ensembli | ENSMUST00000032561; ENSMUSP00000032561; ENSMUSG00000030403 |
GeneIDi | 22323 |
KEGGi | mmu:22323 |
UCSCi | uc009fle.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X98475 Genomic DNA Translation: CAA67108.1 Sequence problems. AF084548 mRNA Translation: AAD16045.1 AK140329 mRNA Translation: BAE24338.1 AK150103 mRNA Translation: BAE29310.1 AK157021 mRNA Translation: BAE33933.1 AK170780 mRNA Translation: BAE42025.1 AK171715 mRNA Translation: BAE42628.1 BC010223 mRNA Translation: AAH10223.1 BC015289 mRNA Translation: AAH15289.1 |
CCDSi | CCDS52056.1 |
RefSeqi | NP_001268950.1, NM_001282021.1 NP_001268951.1, NM_001282022.1 NP_033525.2, NM_009499.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2V8C | X-ray | 1.98 | C | 165-184 | [»] | |
SMRi | P70460 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 204499, 6 interactors |
DIPi | DIP-29360N |
IntActi | P70460, 5 interactors |
MINTi | P70460 |
STRINGi | 10090.ENSMUSP00000032561 |
PTM databases
iPTMneti | P70460 |
PhosphoSitePlusi | P70460 |
SwissPalmi | P70460 |
Proteomic databases
EPDi | P70460 |
jPOSTi | P70460 |
MaxQBi | P70460 |
PaxDbi | P70460 |
PeptideAtlasi | P70460 |
PRIDEi | P70460 |
Protocols and materials databases
Antibodypediai | 1491, 1061 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000032561; ENSMUSP00000032561; ENSMUSG00000030403 |
GeneIDi | 22323 |
KEGGi | mmu:22323 |
UCSCi | uc009fle.2, mouse |
Organism-specific databases
CTDi | 7408 |
MGIi | MGI:109268, Vasp |
Phylogenomic databases
eggNOGi | KOG4590, Eukaryota |
GeneTreei | ENSGT00940000156765 |
HOGENOMi | CLU_017790_0_0_1 |
InParanoidi | P70460 |
OMAi | IRGVKYN |
OrthoDBi | 972128at2759 |
PhylomeDBi | P70460 |
TreeFami | TF321411 |
Enzyme and pathway databases
Reactomei | R-MMU-376176, Signaling by ROBO receptors R-MMU-446353, Cell-extracellular matrix interactions |
Miscellaneous databases
BioGRID-ORCSi | 22323, 1 hit in 17 CRISPR screens |
ChiTaRSi | Vasp, mouse |
PROi | PR:P70460 |
RNActi | P70460, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000030403, Expressed in granulocyte and 291 other tissues |
Genevisiblei | P70460, MM |
Family and domain databases
Gene3Di | 1.20.5.1160, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR042180, IF_rod_dom_coil1B IPR011993, PH-like_dom_sf IPR034367, VASP IPR017354, VASP/EVL IPR038023, VASP_sf IPR014885, VASP_tetra IPR000697, WH1/EVH1_dom |
PANTHERi | PTHR11202:SF12, PTHR11202:SF12, 1 hit |
Pfami | View protein in Pfam PF08776, VASP_tetra, 1 hit PF00568, WH1, 1 hit |
PIRSFi | PIRSF038010, Vasodilator_Phospo, 1 hit |
SMARTi | View protein in SMART SM00461, WH1, 1 hit |
SUPFAMi | SSF118370, SSF118370, 1 hit |
PROSITEi | View protein in PROSITE PS50229, WH1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VASP_MOUSE | |
Accessioni | P70460Primary (citable) accession number: P70460 Secondary accession number(s): Q3TAP0 Q9R214 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 172 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families