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Protein

Exostosin-2

Gene

Ext2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Glycosyltransferase required for the biosynthesis of heparan-sulfate. The EXT1/EXT2 complex possesses substantially higher glycosyltransferase activity than EXT1 or EXT2 alone. Appears to be a tumor suppressor. Required for the exosomal release of SDCBP, CD63 and syndecan.By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan = UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan.
UDP-alpha-D-glucuronate + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei490SubstrateBy similarity1
Metal bindingi540Manganese; catalyticBy similarity1
Binding sitei569SubstrateBy similarity1
Active sitei628By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandManganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-2022928 HS-GAG biosynthesis
UniPathwayi
UPA00378

Protein family/group databases

CAZyiGT47 Glycosyltransferase Family 47
GT64 Glycosyltransferase Family 64

Names & Taxonomyi

Protein namesi
Recommended name:
Exostosin-2 (EC:2.4.1.224, EC:2.4.1.225)
Alternative name(s):
Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase
Multiple exostoses protein 2 homolog
Gene namesi
Name:Ext2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:108050 Ext2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 25CytoplasmicSequence analysisAdd BLAST25
Transmembranei26 – 46Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini47 – 718LumenalSequence analysisAdd BLAST672

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001496521 – 718Exostosin-2Add BLAST718

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi288N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi626 ↔ 676By similarity
Glycosylationi637N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiP70428
MaxQBiP70428
PaxDbiP70428
PeptideAtlasiP70428
PRIDEiP70428

PTM databases

iPTMnetiP70428
PhosphoSitePlusiP70428

Expressioni

Tissue specificityi

Expressed in heart, brain, spleen, lung, liver, skeletal muscle and testis. Heart shows a high expression.2 Publications

Developmental stagei

Expressed in early stages of embryonic development.

Gene expression databases

BgeeiENSMUSG00000027198 Expressed in 280 organ(s), highest expression level in fibroblast
CleanExiMM_EXT2
ExpressionAtlasiP70428 baseline and differential
GenevisibleiP70428 MM

Interactioni

Subunit structurei

Interacts with GALNT5 (By similarity). Forms a homo/hetero-oligomeric complex with EXT1. Interacts with NDST1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Ndst1Q3UHN92EBI-15693102,EBI-15693148

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-29858N
IntActiP70428, 1 interactor
STRINGi10090.ENSMUSP00000028623

Structurei

3D structure databases

ProteinModelPortaliP70428
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni517 – 522Substrate bindingBy similarity6
Regioni538 – 540Substrate bindingBy similarity3
Regioni624 – 628Substrate bindingBy similarity5
Regioni662 – 673Substrate bindingBy similarityAdd BLAST12

Sequence similaritiesi

Belongs to the glycosyltransferase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1021 Eukaryota
ENOG410XTFH LUCA
GeneTreeiENSGT00550000074496
HOVERGENiHBG101211
InParanoidiP70428
KOiK02367
OMAiAFHHKYW
OrthoDBiEOG091G02GW
TreeFamiTF314231

Family and domain databases

Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR004263 Exostosin
IPR027673 Exostosin-2
IPR015338 EXT_C
IPR029044 Nucleotide-diphossugar_trans
PANTHERiPTHR11062 PTHR11062, 1 hit
PTHR11062:SF128 PTHR11062:SF128, 1 hit
PfamiView protein in Pfam
PF03016 Exostosin, 1 hit
PF09258 Glyco_transf_64, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All

P70428-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MCASVKSNIR GPALIPRMKT KHRIYYVTLF SIVLLGLIAT GMFQFWPHSI
60 70 80 90 100
ESSSDGGVEK RSIREVPVVR LPTDSPIPER GDLSCRMHTC FDVYRCGFNP
110 120 130 140 150
KNKIKVYIYP LKKYVDDAGV PVSSAISREY NELLTAISDS DYYTDDINRA
160 170 180 190 200
CLFVPSIDVL NQNPLRIKET AQALAQLSRW DRGTNHLLFN MLPGAPPDYN
210 220 230 240 250
TALDVPRDRA LLAGGGFSTW TYRQGYDVSI PVFSPLSAEM ALPEKAPGPR
260 270 280 290 300
RYFLLSSQMA IHPEYREELE ALQAKHQESV LVLDKCTNLS EGVLSVRKRC
310 320 330 340 350
HQHQVFDYPQ VLQEATFCTV LRGARLGQAV LSDVLQAGCV PVVIADSYIL
360 370 380 390 400
PFSEVLDWKR ASVVVPEEKM SDVYSILQNI PQRQIEEMQR QARWFWEAYF
410 420 430 440 450
QSIKAIALAT LQIINDRIYP YAAISYEEWN DPPAVKWASV SNPLFLPLIP
460 470 480 490 500
PQSQGFTAIV LTYDRVESLF RVITEVSKVP SLSKLLVVWN NQNKNPPEES
510 520 530 540 550
LWPKIRVPLK VVRTAENKLS NRFFPYDEIE TEAVLAIDDD IIMLTSDELQ
560 570 580 590 600
FGYEVWREFP DRLVGYPGRL HLWDHEMNKW KYESEWTNEV SMVLTGAAFY
610 620 630 640 650
HKYFNYLYTY KMPGDIKNWV DTHMNCEDIA MNFLVANVTG KAVIKVTPRK
660 670 680 690 700
KFKCPECTAI DGLSLDQTHM VERSECINKF ASVFGTMPLK VVEHRADPVL
710
YKDDFPEKLK SFPNIGSL
Length:718
Mass (Da):82,064
Last modified:July 27, 2011 - v2
Checksum:i45D947BF6678378B
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2AIG6A2AIG6_MOUSE
Exostosin-2
Ext2
122Annotation score:
V9GX32V9GX32_MOUSE
Exostosin-2
Ext2
586Annotation score:
A2AIG3A2AIG3_MOUSE
Exostosin-2
Ext2
213Annotation score:
E9Q1M5E9Q1M5_MOUSE
Exostosin-2
Ext2
407Annotation score:
F6WHC9F6WHC9_MOUSE
Exostosin-2
Ext2
100Annotation score:
B0QZZ7B0QZZ7_MOUSE
Exostosin-2
Ext2
20Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti62S → T in AAC53143 (PubMed:9110175).Curated1
Sequence conflicti160L → V in AAC53143 (PubMed:9110175).Curated1
Sequence conflicti323G → R in AAC53143 (PubMed:9110175).Curated1
Sequence conflicti355V → I in AAC53143 (PubMed:9110175).Curated1
Sequence conflicti360R → K in AAC53143 (PubMed:9110175).Curated1
Sequence conflicti389Q → H in AAC53143 (PubMed:9110175).Curated1
Sequence conflicti622T → A in AAC53143 (PubMed:9110175).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72141 mRNA Translation: AAB17006.1
U67837 mRNA Translation: AAC53143.1
AK164342 mRNA Translation: BAE37749.1
AL732493, AL732472 Genomic DNA Translation: CAM22097.1
AL732472, AL732493 Genomic DNA Translation: CAM23881.1
CH466519 Genomic DNA Translation: EDL27629.1
BC006597 mRNA Translation: AAH06597.1
CCDSiCCDS16456.1
RefSeqiNP_034293.2, NM_010163.3
XP_006498796.1, XM_006498733.3
UniGeneiMm.4336

Genome annotation databases

EnsembliENSMUST00000028623; ENSMUSP00000028623; ENSMUSG00000027198
GeneIDi14043
KEGGimmu:14043
UCSCiuc008lgf.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72141 mRNA Translation: AAB17006.1
U67837 mRNA Translation: AAC53143.1
AK164342 mRNA Translation: BAE37749.1
AL732493, AL732472 Genomic DNA Translation: CAM22097.1
AL732472, AL732493 Genomic DNA Translation: CAM23881.1
CH466519 Genomic DNA Translation: EDL27629.1
BC006597 mRNA Translation: AAH06597.1
CCDSiCCDS16456.1
RefSeqiNP_034293.2, NM_010163.3
XP_006498796.1, XM_006498733.3
UniGeneiMm.4336

3D structure databases

ProteinModelPortaliP70428
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29858N
IntActiP70428, 1 interactor
STRINGi10090.ENSMUSP00000028623

Protein family/group databases

CAZyiGT47 Glycosyltransferase Family 47
GT64 Glycosyltransferase Family 64

PTM databases

iPTMnetiP70428
PhosphoSitePlusiP70428

Proteomic databases

EPDiP70428
MaxQBiP70428
PaxDbiP70428
PeptideAtlasiP70428
PRIDEiP70428

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028623; ENSMUSP00000028623; ENSMUSG00000027198
GeneIDi14043
KEGGimmu:14043
UCSCiuc008lgf.2 mouse

Organism-specific databases

CTDi2132
MGIiMGI:108050 Ext2

Phylogenomic databases

eggNOGiKOG1021 Eukaryota
ENOG410XTFH LUCA
GeneTreeiENSGT00550000074496
HOVERGENiHBG101211
InParanoidiP70428
KOiK02367
OMAiAFHHKYW
OrthoDBiEOG091G02GW
TreeFamiTF314231

Enzyme and pathway databases

UniPathwayi
UPA00378

ReactomeiR-MMU-2022928 HS-GAG biosynthesis

Miscellaneous databases

ChiTaRSiExt2 mouse
PROiPR:P70428
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027198 Expressed in 280 organ(s), highest expression level in fibroblast
CleanExiMM_EXT2
ExpressionAtlasiP70428 baseline and differential
GenevisibleiP70428 MM

Family and domain databases

Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR004263 Exostosin
IPR027673 Exostosin-2
IPR015338 EXT_C
IPR029044 Nucleotide-diphossugar_trans
PANTHERiPTHR11062 PTHR11062, 1 hit
PTHR11062:SF128 PTHR11062:SF128, 1 hit
PfamiView protein in Pfam
PF03016 Exostosin, 1 hit
PF09258 Glyco_transf_64, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEXT2_MOUSE
AccessioniPrimary (citable) accession number: P70428
Secondary accession number(s): P70395, Q3TPI7, Q923D6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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