UniProtKB - P70351 (EZH1_MOUSE)
Histone-lysine N-methyltransferase EZH1
Ezh1
Functioni
Catalytic activityi
- L-lysyl27-[histone H3] + 3 S-adenosyl-L-methionine = 3 H+ + N6,N6,N6-trimethyl-L-lysyl27-[histone H3] + 3 S-adenosyl-L-homocysteine1 PublicationEC:2.1.1.3561 Publication
GO - Molecular functioni
- chromatin binding Source: MGI
- histone methyltransferase activity (H3-K27 specific) Source: MGI
- transcription corepressor activity Source: ARUK-UCL
GO - Biological processi
- chromatin remodeling Source: ARUK-UCL
- chromatin silencing at telomere Source: ARUK-UCL
- hepatocyte homeostasis Source: MGI
- heterochromatin assembly Source: ARUK-UCL
- histone H3-K27 trimethylation Source: MGI
- liver regeneration Source: MGI
- negative regulation of transcription by RNA polymerase II Source: ARUK-UCL
- positive regulation of transcription by RNA polymerase II Source: MGI
- response to tetrachloromethane Source: MGI
Keywordsi
Molecular function | Chromatin regulator, Methyltransferase, Repressor, Transferase |
Biological process | Transcription, Transcription regulation |
Ligand | S-adenosyl-L-methionine |
Names & Taxonomyi
Protein namesi | Recommended name: Histone-lysine N-methyltransferase EZH1 (EC:2.1.1.3561 Publication)Alternative name(s): ENX-2 Enhancer of zeste homolog 1 |
Gene namesi | Name:Ezh1 Synonyms:Enx2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1097695, Ezh1 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Note: Colocalizes with trimethylated 'Lys-27' of histone H3.By similarity
Nucleus
- ESC/E(Z) complex Source: UniProtKB
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- chromosome, telomeric region Source: ARUK-UCL
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000213991 | 1 – 747 | Histone-lysine N-methyltransferase EZH1Add BLAST | 747 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 327 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
EPDi | P70351 |
MaxQBi | P70351 |
PaxDbi | P70351 |
PeptideAtlasi | P70351 |
PRIDEi | P70351 |
PTM databases
iPTMneti | P70351 |
PhosphoSitePlusi | P70351 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000006920, Expressed in adult mammalian kidney and 293 other tissues |
ExpressionAtlasi | P70351, baseline and differential |
Genevisiblei | P70351, MM |
Interactioni
Subunit structurei
Component of the PRC2/EED-EZH1 complex, which includes EED, EZH1, SUZ12, RBBP4 and AEBP2. The PRC2/EED-EZH1 is less abundant than the PRC2/EED-EZH2 complex, has weak methyltransferase activity and compacts chromatin in the absence of the methyltransferase cofactor S-adenosyl-L-methionine (SAM).
Interacts with EZHIP; the interaction blocks EZH1 methyltransferase activity (PubMed:31451685).
3 PublicationsBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 199563, 19 interactors |
DIPi | DIP-56992N |
IntActi | P70351, 8 interactors |
STRINGi | 10090.ENSMUSP00000102906 |
Miscellaneous databases
RNActi | P70351, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 504 – 606 | CXCPROSITE-ProRule annotationAdd BLAST | 103 | |
Domaini | 613 – 728 | SETPROSITE-ProRule annotationAdd BLAST | 116 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 491 – 496 | Nuclear localization signalSequence analysis | 6 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 524 – 606 | Cys-richAdd BLAST | 83 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1079, Eukaryota |
GeneTreei | ENSGT00940000156604 |
HOGENOMi | CLU_011342_0_0_1 |
InParanoidi | P70351 |
OrthoDBi | 875190at2759 |
PhylomeDBi | P70351 |
TreeFami | TF314509 |
Family and domain databases
CDDi | cd00167, SANT, 1 hit |
InterProi | View protein in InterPro IPR026489, CXC_dom IPR032926, EZH1 IPR021654, EZH1/EZH2 IPR041343, PRC2_HTH_1 IPR041355, Pre-SET_CXC IPR001005, SANT/Myb IPR001214, SET_dom IPR033467, Tesmin/TSO1-like_CXC |
PANTHERi | PTHR45747:SF1, PTHR45747:SF1, 1 hit |
Pfami | View protein in Pfam PF11616, EZH2_WD-Binding, 1 hit PF18118, PRC2_HTH_1, 1 hit PF18264, preSET_CXC, 1 hit PF00856, SET, 1 hit |
SMARTi | View protein in SMART SM01114, CXC, 1 hit SM00717, SANT, 2 hits SM00317, SET, 1 hit |
PROSITEi | View protein in PROSITE PS51633, CXC, 1 hit PS50280, SET, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDIASPPTSK CITYWKRKVK SEYMRLRQLK RLQANMGAKA LYVANFAKVQ
60 70 80 90 100
EKTQILNEEW KKLRVQPVQP MKPVSGHPFL KKCTIESIFP GFDSQDMLMR
110 120 130 140 150
SLNTVALVPI MYSWSPLQQN FMVEDETVLC NIPYMGDEVK EEDETFIEEL
160 170 180 190 200
INNYDGKVHG EEEMIPGSVL ISDAVFLELV DALNQYSDEE EDGHNDPSDG
210 220 230 240 250
KQDDSKEDLP VTRKRKRHAI EGNKKSSKKQ FPNDMIFSAI ASMFPENGVP
260 270 280 290 300
DDMKERYREL TEMSDPNALP PQCTPNIDGP NAKSVQREQS LHSFHTLFCR
310 320 330 340 350
RCFKYDCFLH PFHATPNVYK RKNKEIKIEP EPCGTDCFLL LEGAKEYAML
360 370 380 390 400
HNPRSKCSGR RRRRHPVVSA SCSNASASAM AETKEGDSDR DTGNDWASSS
410 420 430 440 450
SEANSRCQTP TKQKASPAPA QLCVVEAPSE PVEWTGAEES LFRVFHGTYF
460 470 480 490 500
NNFCSIARLL GTKTCKQVFQ FAVKESLILK LPTDELMNPA QKKKRKHRLW
510 520 530 540 550
AAHCRKIQLK KDNNSTQVYN YQPCDHPDRP CDSTCPCIMT QNFCEKFCQC
560 570 580 590 600
SPDCQNRFPG CRCKTQCNTK QCPCYLAVRE CDPDLCLTCG ASEHWDCKVV
610 620 630 640 650
SCKNCSIQRG LKKHLLLAPS DVAGWGTFIK ESVQKNEFIS EYCGELISQD
660 670 680 690 700
EADRRGKVYD KYMSSFLFNL NNDFVVDATR KGNKIRFANH SVNPNCYAKV
710 720 730 740
VMVNGDHRIG IFAKRAIQAG EELFFDYRYS QADALKYVGI ERETDVF
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0R4J1C0 | A0A0R4J1C0_MOUSE | [Histone H3]-lysine(27) N-trimethyl... | Ezh1 | 750 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 84 | T → A in BAE32680 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 446 | H → Y in AAD54021 (PubMed:9473645).Curated | 1 | |
Sequence conflicti | 452 | N → Y in AAD54021 (PubMed:9473645).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_036388 | 555 – 747 | QNRFP…ETDVF → KSTLLSPSSTQVVGLGVPRL FSPAP in isoform 2. 1 PublicationAdd BLAST | 193 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U60453 mRNA Translation: AAC53279.1 AB004817 mRNA Translation: BAA25018.1 Different initiation. AF104360 Genomic DNA Translation: AAD54021.1 AF483490 mRNA Translation: AAL90764.1 Different initiation. AF483491 mRNA Translation: AAL90765.1 Different initiation. AK045374 mRNA Translation: BAC32334.1 AK138942 mRNA Translation: BAE23827.1 Different initiation. AK140694 mRNA Translation: BAE24446.1 Different initiation. AK154565 mRNA Translation: BAE32680.1 Different initiation. AK164192 mRNA Translation: BAE37674.1 Different initiation. AL590969 Genomic DNA No translation available. CH466677 Genomic DNA Translation: EDL03899.1 Different initiation. BC007135 mRNA Translation: AAH07135.1 Different initiation. |
RefSeqi | NP_031996.1, NM_007970.3 XP_006532241.1, XM_006532178.3 [P70351-1] |
Genome annotation databases
Ensembli | ENSMUST00000100417; ENSMUSP00000097984; ENSMUSG00000006920 [P70351-2] ENSMUST00000107284; ENSMUSP00000102905; ENSMUSG00000006920 [P70351-1] |
GeneIDi | 14055 |
KEGGi | mmu:14055 |
UCSCi | uc007lnw.3, mouse [P70351-2] uc011yfk.2, mouse [P70351-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U60453 mRNA Translation: AAC53279.1 AB004817 mRNA Translation: BAA25018.1 Different initiation. AF104360 Genomic DNA Translation: AAD54021.1 AF483490 mRNA Translation: AAL90764.1 Different initiation. AF483491 mRNA Translation: AAL90765.1 Different initiation. AK045374 mRNA Translation: BAC32334.1 AK138942 mRNA Translation: BAE23827.1 Different initiation. AK140694 mRNA Translation: BAE24446.1 Different initiation. AK154565 mRNA Translation: BAE32680.1 Different initiation. AK164192 mRNA Translation: BAE37674.1 Different initiation. AL590969 Genomic DNA No translation available. CH466677 Genomic DNA Translation: EDL03899.1 Different initiation. BC007135 mRNA Translation: AAH07135.1 Different initiation. |
RefSeqi | NP_031996.1, NM_007970.3 XP_006532241.1, XM_006532178.3 [P70351-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 199563, 19 interactors |
DIPi | DIP-56992N |
IntActi | P70351, 8 interactors |
STRINGi | 10090.ENSMUSP00000102906 |
PTM databases
iPTMneti | P70351 |
PhosphoSitePlusi | P70351 |
Proteomic databases
EPDi | P70351 |
MaxQBi | P70351 |
PaxDbi | P70351 |
PeptideAtlasi | P70351 |
PRIDEi | P70351 |
Protocols and materials databases
Antibodypediai | 1432, 389 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000100417; ENSMUSP00000097984; ENSMUSG00000006920 [P70351-2] ENSMUST00000107284; ENSMUSP00000102905; ENSMUSG00000006920 [P70351-1] |
GeneIDi | 14055 |
KEGGi | mmu:14055 |
UCSCi | uc007lnw.3, mouse [P70351-2] uc011yfk.2, mouse [P70351-1] |
Organism-specific databases
CTDi | 2145 |
MGIi | MGI:1097695, Ezh1 |
Phylogenomic databases
eggNOGi | KOG1079, Eukaryota |
GeneTreei | ENSGT00940000156604 |
HOGENOMi | CLU_011342_0_0_1 |
InParanoidi | P70351 |
OrthoDBi | 875190at2759 |
PhylomeDBi | P70351 |
TreeFami | TF314509 |
Miscellaneous databases
BioGRID-ORCSi | 14055, 1 hit in 19 CRISPR screens |
ChiTaRSi | Ezh1, mouse |
PROi | PR:P70351 |
RNActi | P70351, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000006920, Expressed in adult mammalian kidney and 293 other tissues |
ExpressionAtlasi | P70351, baseline and differential |
Genevisiblei | P70351, MM |
Family and domain databases
CDDi | cd00167, SANT, 1 hit |
InterProi | View protein in InterPro IPR026489, CXC_dom IPR032926, EZH1 IPR021654, EZH1/EZH2 IPR041343, PRC2_HTH_1 IPR041355, Pre-SET_CXC IPR001005, SANT/Myb IPR001214, SET_dom IPR033467, Tesmin/TSO1-like_CXC |
PANTHERi | PTHR45747:SF1, PTHR45747:SF1, 1 hit |
Pfami | View protein in Pfam PF11616, EZH2_WD-Binding, 1 hit PF18118, PRC2_HTH_1, 1 hit PF18264, preSET_CXC, 1 hit PF00856, SET, 1 hit |
SMARTi | View protein in SMART SM01114, CXC, 1 hit SM00717, SANT, 2 hits SM00317, SET, 1 hit |
PROSITEi | View protein in PROSITE PS51633, CXC, 1 hit PS50280, SET, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EZH1_MOUSE | |
Accessioni | P70351Primary (citable) accession number: P70351 Secondary accession number(s): A2A4K5 Q9R089 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | February 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 161 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families