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Protein

Histidine triad nucleotide-binding protein 1

Gene

Hint1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes purine nucleotide phosphoramidates with a single phosphate group, including adenosine 5'monophosphoramidate (AMP-NH2), adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His-AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester. Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O-phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide. In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1. Modulates p53/TP53 levels and p53/TP53-mediated apoptosis. Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin-protein ligase complex (By similarity).By similarity

Caution

Was originally thought to be a protein kinase C inhibitor and to bind zinc in solution. Both seem to be incorrect.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei99Purine nucleotide phosphoramidateBy similarity1
Active sitei112Tele-AMP-histidine intermediateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi43 – 44Purine nucleotide phosphoramidateBy similarity2
Nucleotide bindingi105 – 107Purine nucleotide phosphoramidateBy similarity3
Nucleotide bindingi112 – 114Purine nucleotide phosphoramidateBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processApoptosis, Transcription, Transcription regulation
LigandNucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine triad nucleotide-binding protein 1 (EC:3.-.-.-)
Alternative name(s):
Adenosine 5'-monophosphoramidase
Protein kinase C inhibitor 1
Protein kinase C-interacting protein 1
Short name:
PKCI-1
Gene namesi
Name:Hint1
Synonyms:Hint, Pkci, Pkci1, Prkcnh1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1321133 Hint1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Mice display increased susceptibility to carcinogens.1 Publication

Chemistry databases

ChEMBLiCHEMBL1250364

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001097822 – 126Histidine triad nucleotide-binding protein 1Add BLAST125

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei21N6-acetyllysineCombined sources1
Modified residuei30N6-acetyllysineBy similarity1
Modified residuei45PhosphoserineCombined sources1
Modified residuei72PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP70349
MaxQBiP70349
PaxDbiP70349
PeptideAtlasiP70349
PRIDEiP70349

2D gel databases

REPRODUCTION-2DPAGEiP70349

PTM databases

iPTMnetiP70349
PhosphoSitePlusiP70349
SwissPalmiP70349

Expressioni

Gene expression databases

BgeeiENSMUSG00000020267 Expressed in 287 organ(s), highest expression level in indifferent gonad
CleanExiMM_HINT1
ExpressionAtlasiP70349 baseline and differential
GenevisibleiP70349 MM

Interactioni

Subunit structurei

Homodimer. Interacts with CDK7. Interacts with RUVBL1 and RUVBL2 and is associated with the LEF1/TCF1-CTNNB1 complex and with a KAT5 histone acetyltransferase complex. Identified in a complex with MITF and CTNNB1. Interacts with CDC34 and RBX1, and is part of a SCF (SKP2-CUL1-F-box protein) E3 ubiquitin-protein ligase complex (By similarity).By similarity

Protein-protein interaction databases

BioGridi200305, 2 interactors
IntActiP70349, 4 interactors
MINTiP70349
STRINGi10090.ENSMUSP00000020504

Structurei

3D structure databases

ProteinModelPortaliP70349
SMRiP70349
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 126HITPROSITE-ProRule annotationAdd BLAST109

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi110 – 114Histidine triad motif5

Sequence similaritiesi

Belongs to the HINT family.Curated

Phylogenomic databases

eggNOGiKOG3275 Eukaryota
COG0537 LUCA
GeneTreeiENSGT00510000046448
HOGENOMiHOG000061064
HOVERGENiHBG051906
InParanoidiP70349
KOiK02503
OMAiDQSIFHT
OrthoDBiEOG091G1001
PhylomeDBiP70349
TreeFamiTF314862

Family and domain databases

Gene3Di3.30.428.10, 1 hit
InterProiView protein in InterPro
IPR019808 Histidine_triad_CS
IPR001310 Histidine_triad_HIT
IPR011146 HIT-like
IPR036265 HIT-like_sf
PANTHERiPTHR23089 PTHR23089, 1 hit
PfamiView protein in Pfam
PF01230 HIT, 1 hit
PRINTSiPR00332 HISTRIAD
SUPFAMiSSF54197 SSF54197, 1 hit
PROSITEiView protein in PROSITE
PS00892 HIT_1, 1 hit
PS51084 HIT_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P70349-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MADEIAKAQV AQPGGDTIFG KIIRKEIPAK IIFEDDRCLA FHDISPQAPT
60 70 80 90 100
HFLVIPKKHI SQISVADDDD ESLLGHLMIV GKKCAADLGL KRGYRMVVNE
110 120
GADGGQSVYH IHLHVLGGRQ MNWPPG
Length:126
Mass (Da):13,777
Last modified:January 23, 2007 - v3
Checksum:i30DA241476389805
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B0R1E3B0R1E3_MOUSE
Histidine triad nucleotide-binding ...
Hint1
119Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60001 mRNA Translation: AAC71076.1
AK002965 mRNA Translation: BAB22484.1
AK012433 mRNA Translation: BAB28235.1
BC070415 mRNA Translation: AAH70415.1
BC080296 mRNA Translation: AAH80296.1
CCDSiCCDS24702.1
PIRiPN0044
RefSeqiNP_032274.1, NM_008248.2
UniGeneiMm.425

Genome annotation databases

EnsembliENSMUST00000020504; ENSMUSP00000020504; ENSMUSG00000020267
GeneIDi15254
KEGGimmu:15254
UCSCiuc007iyj.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60001 mRNA Translation: AAC71076.1
AK002965 mRNA Translation: BAB22484.1
AK012433 mRNA Translation: BAB28235.1
BC070415 mRNA Translation: AAH70415.1
BC080296 mRNA Translation: AAH80296.1
CCDSiCCDS24702.1
PIRiPN0044
RefSeqiNP_032274.1, NM_008248.2
UniGeneiMm.425

3D structure databases

ProteinModelPortaliP70349
SMRiP70349
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200305, 2 interactors
IntActiP70349, 4 interactors
MINTiP70349
STRINGi10090.ENSMUSP00000020504

Chemistry databases

ChEMBLiCHEMBL1250364

PTM databases

iPTMnetiP70349
PhosphoSitePlusiP70349
SwissPalmiP70349

2D gel databases

REPRODUCTION-2DPAGEiP70349

Proteomic databases

EPDiP70349
MaxQBiP70349
PaxDbiP70349
PeptideAtlasiP70349
PRIDEiP70349

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020504; ENSMUSP00000020504; ENSMUSG00000020267
GeneIDi15254
KEGGimmu:15254
UCSCiuc007iyj.1 mouse

Organism-specific databases

CTDi3094
MGIiMGI:1321133 Hint1

Phylogenomic databases

eggNOGiKOG3275 Eukaryota
COG0537 LUCA
GeneTreeiENSGT00510000046448
HOGENOMiHOG000061064
HOVERGENiHBG051906
InParanoidiP70349
KOiK02503
OMAiDQSIFHT
OrthoDBiEOG091G1001
PhylomeDBiP70349
TreeFamiTF314862

Miscellaneous databases

PROiPR:P70349
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020267 Expressed in 287 organ(s), highest expression level in indifferent gonad
CleanExiMM_HINT1
ExpressionAtlasiP70349 baseline and differential
GenevisibleiP70349 MM

Family and domain databases

Gene3Di3.30.428.10, 1 hit
InterProiView protein in InterPro
IPR019808 Histidine_triad_CS
IPR001310 Histidine_triad_HIT
IPR011146 HIT-like
IPR036265 HIT-like_sf
PANTHERiPTHR23089 PTHR23089, 1 hit
PfamiView protein in Pfam
PF01230 HIT, 1 hit
PRINTSiPR00332 HISTRIAD
SUPFAMiSSF54197 SSF54197, 1 hit
PROSITEiView protein in PROSITE
PS00892 HIT_1, 1 hit
PS51084 HIT_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHINT1_MOUSE
AccessioniPrimary (citable) accession number: P70349
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 147 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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