UniProtKB - P70340 (SMAD1_MOUSE)
Mothers against decapentaplegic homolog 1
Smad1
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 64 | Zinc | 1 | |
Metal bindingi | 109 | Zinc | 1 | |
Metal bindingi | 121 | Zinc | 1 | |
Metal bindingi | 126 | Zinc | 1 |
GO - Molecular functioni
- co-SMAD binding Source: MGI
- DEAD/H-box RNA helicase binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
- DNA-binding transcription factor activity Source: MGI
- identical protein binding Source: MGI
- I-SMAD binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- primary miRNA binding Source: BHF-UCL
- protein kinase binding Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific DNA binding Source: UniProtKB
- transcription factor binding Source: GO_Central
- ubiquitin protein ligase binding Source: GO_Central
GO - Biological processi
- anatomical structure morphogenesis Source: GO_Central
- BMP signaling pathway Source: MGI
- bone development Source: MGI
- cardiac muscle cell proliferation Source: MGI
- cartilage development Source: MGI
- cell differentiation Source: GO_Central
- cellular response to BMP stimulus Source: BHF-UCL
- cellular response to organic cyclic compound Source: MGI
- embryonic pattern specification Source: UniProtKB
- gamete generation Source: MGI
- hindbrain development Source: MGI
- homeostatic process Source: MGI
- inflammatory response Source: UniProtKB
- MAPK cascade Source: MGI
- mesodermal cell fate commitment Source: MGI
- midbrain development Source: MGI
- negative regulation of cell population proliferation Source: MGI
- osteoblast fate commitment Source: MGI
- positive regulation of cartilage development Source: MGI
- positive regulation of cell differentiation Source: MGI
- positive regulation of gene expression Source: MGI
- positive regulation of osteoblast differentiation Source: MGI
- positive regulation of pri-miRNA transcription by RNA polymerase II Source: BHF-UCL
- positive regulation of sprouting angiogenesis Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus Source: BHF-UCL
- protein phosphorylation Source: MGI
- regulation of transcription by RNA polymerase II Source: MGI
- response to drug Source: MGI
- SMAD protein complex assembly Source: MGI
- SMAD protein signal transduction Source: BHF-UCL
- transforming growth factor beta receptor signaling pathway Source: GO_Central
- ureteric bud development Source: UniProtKB
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-201451, Signaling by BMP R-MMU-5689880, Ub-specific processing proteases R-MMU-8941326, RUNX2 regulates bone development |
Names & Taxonomyi
Protein namesi | Recommended name: Mothers against decapentaplegic homolog 1Short name: MAD homolog 1 Short name: Mothers against DPP homolog 1 Alternative name(s): Dwarfin-A Short name: Dwf-A Mothers-against-DPP-related 1 Short name: Mad-related protein 1 Short name: mMad1 SMAD family member 1 Short name: SMAD 1 Short name: Smad1 |
Gene namesi | Name:Smad1 Synonyms:Madh1, Madr1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:109452, Smad1 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Other locations
- Cytoplasm 1 Publication
Note: In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4. Colocalizes with LEMD3 at the nucleus inner membrane. Exported from the nucleus to the cytoplasm when dephosphorylated (PubMed:25755279).By similarity1 Publication
Nucleus
- heteromeric SMAD protein complex Source: GO_Central
- nuclear inner membrane Source: MGI
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- protein-containing complex Source: MGI
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000090848 | 1 – 465 | Mothers against decapentaplegic homolog 1Add BLAST | 465 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 322 | Phosphothreonine; by MINK1, TNIK and MAP4K4By similarity | 1 | |
Modified residuei | 463 | PhosphoserinePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 465 | PhosphoserinePROSITE-ProRule annotationBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P70340 |
MaxQBi | P70340 |
PaxDbi | P70340 |
PeptideAtlasi | P70340 |
PRIDEi | P70340 |
PTM databases
iPTMneti | P70340 |
PhosphoSitePlusi | P70340 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000031681, Expressed in pineal body and 342 other tissues |
ExpressionAtlasi | P70340, baseline and differential |
Genevisiblei | P70340, MM |
Interactioni
Subunit structurei
Upon C-terminus phosphorylation: forms trimers with another SMAD1 and the co-SMAD SMAD4.
Interacts with PEBP2-alpha subunit, CREB-binding protein (CBP), p300, SMURF1, SMURF2, USP15 and HOXC8. Associates with ZNF423 or ZNF521 in response to BMP2 leading to activate transcription of BMP target genes.
Interacts with SKOR1.
Interacts (via MH2 domain) with LEMD3. Binding to LEMD3 results in at least a partial reduction of receptor-mediated phosphorylation (By similarity).
Also interacts with HGS, NANOG and ZCCHC12.
Forms a ternary complex with PSMB4 and OAZ1 before PSMB4 is incorporated into the 20S proteasome (By similarity).
Found in a complex with SMAD4 and YY1.
Interacts (via MH2 domain) with FAM83G (via MH2 domain); in a SMAD4-independent manner.
Interacts with ZC3H3 (PubMed:16115198).
Interacts with TMEM119 (PubMed:21239498).
Interacts (via MH1 and MH2 domains) with ZNF8 (PubMed:12370310).
Interacts with RANBP3L; the interaction increases when SMAD1 is not phosphorylated and mediates SMAD1 nuclear export (PubMed:25755279).
By similarity9 PublicationsBinary interactionsi
P70340
With | #Exp. | IntAct |
---|---|---|
Map3k7 [Q62073] | 3 | EBI-6992047,EBI-1775345 |
GO - Molecular functioni
- co-SMAD binding Source: MGI
- DEAD/H-box RNA helicase binding Source: MGI
- identical protein binding Source: MGI
- I-SMAD binding Source: MGI
- protein kinase binding Source: MGI
- transcription factor binding Source: GO_Central
- ubiquitin protein ligase binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 201274, 34 interactors |
ComplexPortali | CPX-145, SMAD1 homotrimer CPX-146, SMAD1-SMAD4 complex |
CORUMi | P70340 |
IntActi | P70340, 9 interactors |
MINTi | P70340 |
STRINGi | 10090.ENSMUSP00000071035 |
Miscellaneous databases
RNActi | P70340, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P70340 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P70340 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 12 – 136 | MH1PROSITE-ProRule annotationAdd BLAST | 125 | |
Domaini | 271 – 465 | MH2PROSITE-ProRule annotationAdd BLAST | 195 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 418 – 428 | L3 loopBy similarityAdd BLAST | 11 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 39 – 45 | Poly-Lys | 7 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3701, Eukaryota |
GeneTreei | ENSGT00940000154391 |
HOGENOMi | CLU_026736_0_2_1 |
InParanoidi | P70340 |
OMAi | EVFHCNS |
OrthoDBi | 608001at2759 |
TreeFami | TF314923 |
Family and domain databases
Gene3Di | 2.60.200.10, 1 hit 3.90.520.10, 1 hit |
InterProi | View protein in InterPro IPR013790, Dwarfin IPR003619, MAD_homology1_Dwarfin-type IPR013019, MAD_homology_MH1 IPR017855, SMAD-like_dom_sf IPR001132, SMAD_dom_Dwarfin-type IPR008984, SMAD_FHA_dom_sf IPR036578, SMAD_MH1_sf |
PANTHERi | PTHR13703, PTHR13703, 1 hit |
Pfami | View protein in Pfam PF03165, MH1, 1 hit PF03166, MH2, 1 hit |
SMARTi | View protein in SMART SM00523, DWA, 1 hit SM00524, DWB, 1 hit |
SUPFAMi | SSF49879, SSF49879, 1 hit SSF56366, SSF56366, 1 hit |
PROSITEi | View protein in PROSITE PS51075, MH1, 1 hit PS51076, MH2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MNVTSLFSFT SPAVKRLLGW KQGDEEEKWA EKAVDALVKK LKKKKGAMEE
60 70 80 90 100
LEKALSCPGQ PSNCVTIPRS LDGRLQVSHR KGLPHVIYCR VWRWPDLQSH
110 120 130 140 150
HELKPLECCE FPFGSKQKEV CINPYHYKRV ESPVLPPVLV PRHSEYNPQH
160 170 180 190 200
SLLAQFRNLG QNEPHMPLNA TFPDSFQQPN SHPFPHSPNS SYPNSPGGSS
210 220 230 240 250
STYPHSPTSS DPGSPFQMPA DTPPPAYLPP EDPMAQDGSQ PMDTNMMAPP
260 270 280 290 300
LPAEISRGDV QAVAYEEPKH WCSIVYYELN NRVGEAFHAS STSVLVDGFT
310 320 330 340 350
DPSNNKNRFC LGLLSNVNRN STIENTRRHI GKGVHLYYVG GEVYAECLSD
360 370 380 390 400
SSIFVQSRNC NYHHGFHPTT VCKIPSGCSL KIFNNQEFAQ LLAQSVNHGF
410 420 430 440 450
ETVYELTKMC TIRMSFVKGW GAEYHRQDVT STPCWIEIHL HGPLQWLDKV
460
LTQMGSPHNP ISSVS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ8C3Y6 | Q8C3Y6_MOUSE | Mothers against decapentaplegic hom... | Smad1 | 428 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 95 | P → S in AAC52785 (PubMed:8799132).Curated | 1 | |
Sequence conflicti | 95 | P → S in AAG41407 (PubMed:11111041).Curated | 1 | |
Sequence conflicti | 142 | R → K in AAC52785 (PubMed:8799132).Curated | 1 | |
Sequence conflicti | 142 | R → K in AAG41407 (PubMed:11111041).Curated | 1 | |
Sequence conflicti | 199 – 200 | SS → QG in AAB18256 (PubMed:9076678).Curated | 2 | |
Sequence conflicti | 393 | A → E in AAB18256 (PubMed:9076678).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U58992 mRNA Translation: AAC52785.1 U74359 mRNA Translation: AAB18256.1 AF295768 , AF295763, AF295764, AF295765, AF295766, AF295767 Genomic DNA Translation: AAG41407.1 AK017583 mRNA Translation: BAB30820.1 AK054104 mRNA Translation: BAC35658.1 BC058693 mRNA Translation: AAH58693.1 |
CCDSi | CCDS22437.1 |
RefSeqi | NP_032565.2, NM_008539.3 XP_006530809.1, XM_006530746.3 |
Genome annotation databases
Ensembli | ENSMUST00000066091; ENSMUSP00000071035; ENSMUSG00000031681 |
GeneIDi | 17125 |
KEGGi | mmu:17125 |
UCSCi | uc009mip.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U58992 mRNA Translation: AAC52785.1 U74359 mRNA Translation: AAB18256.1 AF295768 , AF295763, AF295764, AF295765, AF295766, AF295767 Genomic DNA Translation: AAG41407.1 AK017583 mRNA Translation: BAB30820.1 AK054104 mRNA Translation: BAC35658.1 BC058693 mRNA Translation: AAH58693.1 |
CCDSi | CCDS22437.1 |
RefSeqi | NP_032565.2, NM_008539.3 XP_006530809.1, XM_006530746.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3KMP | X-ray | 2.70 | A/B | 9-132 | [»] | |
SMRi | P70340 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 201274, 34 interactors |
ComplexPortali | CPX-145, SMAD1 homotrimer CPX-146, SMAD1-SMAD4 complex |
CORUMi | P70340 |
IntActi | P70340, 9 interactors |
MINTi | P70340 |
STRINGi | 10090.ENSMUSP00000071035 |
Chemistry databases
ChEMBLi | CHEMBL3883282 |
PTM databases
iPTMneti | P70340 |
PhosphoSitePlusi | P70340 |
Proteomic databases
EPDi | P70340 |
MaxQBi | P70340 |
PaxDbi | P70340 |
PeptideAtlasi | P70340 |
PRIDEi | P70340 |
Protocols and materials databases
Antibodypediai | 3950, 1295 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000066091; ENSMUSP00000071035; ENSMUSG00000031681 |
GeneIDi | 17125 |
KEGGi | mmu:17125 |
UCSCi | uc009mip.2, mouse |
Organism-specific databases
CTDi | 4086 |
MGIi | MGI:109452, Smad1 |
Phylogenomic databases
eggNOGi | KOG3701, Eukaryota |
GeneTreei | ENSGT00940000154391 |
HOGENOMi | CLU_026736_0_2_1 |
InParanoidi | P70340 |
OMAi | EVFHCNS |
OrthoDBi | 608001at2759 |
TreeFami | TF314923 |
Enzyme and pathway databases
Reactomei | R-MMU-201451, Signaling by BMP R-MMU-5689880, Ub-specific processing proteases R-MMU-8941326, RUNX2 regulates bone development |
Miscellaneous databases
BioGRID-ORCSi | 17125, 2 hits in 19 CRISPR screens |
ChiTaRSi | Smad1, mouse |
EvolutionaryTracei | P70340 |
PROi | PR:P70340 |
RNActi | P70340, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031681, Expressed in pineal body and 342 other tissues |
ExpressionAtlasi | P70340, baseline and differential |
Genevisiblei | P70340, MM |
Family and domain databases
Gene3Di | 2.60.200.10, 1 hit 3.90.520.10, 1 hit |
InterProi | View protein in InterPro IPR013790, Dwarfin IPR003619, MAD_homology1_Dwarfin-type IPR013019, MAD_homology_MH1 IPR017855, SMAD-like_dom_sf IPR001132, SMAD_dom_Dwarfin-type IPR008984, SMAD_FHA_dom_sf IPR036578, SMAD_MH1_sf |
PANTHERi | PTHR13703, PTHR13703, 1 hit |
Pfami | View protein in Pfam PF03165, MH1, 1 hit PF03166, MH2, 1 hit |
SMARTi | View protein in SMART SM00523, DWA, 1 hit SM00524, DWB, 1 hit |
SUPFAMi | SSF49879, SSF49879, 1 hit SSF56366, SSF56366, 1 hit |
PROSITEi | View protein in PROSITE PS51075, MH1, 1 hit PS51076, MH2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SMAD1_MOUSE | |
Accessioni | P70340Primary (citable) accession number: P70340 Secondary accession number(s): P70442, Q6GT95, Q9CYK6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 10, 2021 | |
This is version 197 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families