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Protein

MyoD family inhibitor

Gene

Mdfi

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways.4 Publications

GO - Molecular functioni

  • identical protein binding Source: MGI
  • transcription factor binding Source: UniProtKB

GO - Biological processi

  • activation of JUN kinase activity Source: UniProtKB
  • dorsal/ventral axis specification Source: UniProtKB
  • embryonic skeletal system morphogenesis Source: MGI
  • negative regulation of DNA binding Source: UniProtKB
  • negative regulation of transcription by RNA polymerase II Source: UniProtKB
  • negative regulation of Wnt signaling pathway Source: UniProtKB
  • regulation of Wnt signaling pathway Source: UniProtKB
  • trophoblast giant cell differentiation Source: MGI

Keywordsi

Molecular functionDevelopmental protein
Biological processDifferentiation

Names & Taxonomyi

Protein namesi
Recommended name:
MyoD family inhibitor
Alternative name(s):
Myogenic repressor I-mf
Gene namesi
Name:Mdfi
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:107687 Mdfi

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000963251 – 251MyoD family inhibitorAdd BLAST251

Proteomic databases

PaxDbiP70331
PRIDEiP70331

PTM databases

iPTMnetiP70331
PhosphoSitePlusiP70331

Expressioni

Tissue specificityi

In the embryo, highly expressed in the sclerotome. Also expressed in the notochord, neural tube, limb buds, heart, branchial arches and head mesenchyme. In the adult, highly expressed in skeletal muscle. Expressed at lower levels in most other tissues.1 Publication

Gene expression databases

BgeeiENSMUSG00000032717 Expressed in 160 organ(s), highest expression level in cardiac ventricle
CleanExiMM_MDFI
ExpressionAtlasiP70331 baseline and differential
GenevisibleiP70331 MM

Interactioni

Subunit structurei

The C-terminus interacts with AXIN1 and LEF1 (PubMed:12192039). Interacts with CCNT2 (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201368, 2 interactors
STRINGi10090.ENSMUSP00000037888

Structurei

3D structure databases

ProteinModelPortaliP70331
SMRiP70331
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MDFI family.Curated

Phylogenomic databases

eggNOGiENOG410IYH9 Eukaryota
ENOG410YK34 LUCA
GeneTreeiENSGT00390000010600
HOGENOMiHOG000001577
InParanoidiP70331
OrthoDBiEOG091G0RJH
PhylomeDBiP70331
TreeFamiTF332113

Family and domain databases

InterProiView protein in InterPro
IPR026134 MDFI/MDFIC
PANTHERiPTHR15304 PTHR15304, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform I-mfB (identifier: P70331-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSQVSGQCPS RCDAPHGVPS AALDPAQTMS LLPGLEVARS THPVEASSEE
60 70 80 90 100
GFPEEAAPSM PHDSGLRAQQ ALNSIDLDVP TEAVTCQPQG NPQGCTPLLP
110 120 130 140 150
NGSSHDHLSE PGSAGHAGNG ALGGSKAHRK LQTHPSLGSQ AGRKSRGSAR
160 170 180 190 200
SASQVPLQAQ EGKAPAVRIH RQTASPTCCL RNAQLSGTAL RSLRLESQGH
210 220 230 240 250
RELNNKTLSQ SNNKKPGVAA HAAIIPALTR PKQNCHDPSL LPGTHGVGKE

F
Length:251
Mass (Da):26,013
Last modified:February 1, 1997 - v1
Checksum:iE77D693D935F1B83
GO
Isoform I-mfA (identifier: P70331-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     162-251: GKAPAVRIHR...PGTHGVGKEF → DCCVHCILSC...MECCGLCFSS

Show »
Length:246
Mass (Da):25,270
Checksum:i138A37A2B4A10463
GO
Isoform I-mfC (identifier: P70331-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     136-163: SLGSQAGRKSRGSARSASQVPLQAQEGK → PQPPNRSAFRRVAWSPPRVTRNTASRAW
     164-251: Missing.

Show »
Length:163
Mass (Da):17,012
Checksum:iDD58E66BF9793D27
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z6Q8D3Z6Q8_MOUSE
MyoD family inhibitor
Mdfi
186Annotation score:
D3YZ53D3YZ53_MOUSE
MyoD family inhibitor
Mdfi
143Annotation score:
D3Z3N7D3Z3N7_MOUSE
MyoD family inhibitor
Mdfi
153Annotation score:
D3YVA0D3YVA0_MOUSE
MyoD family inhibitor
Mdfi
124Annotation score:
D3YUT8D3YUT8_MOUSE
MyoD family inhibitor
Mdfi
68Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004055136 – 163SLGSQ…AQEGK → PQPPNRSAFRRVAWSPPRVT RNTASRAW in isoform I-mfC. 1 PublicationAdd BLAST28
Alternative sequenceiVSP_004056162 – 251GKAPA…VGKEF → DCCVHCILSCLFCEFLTLCN ILLDCATCGSCSSEDSCLCC CCCGSGECADCDLPCDLDCG IVDACCESADCLEICMECCG LCFSS in isoform I-mfA. 2 PublicationsAdd BLAST90
Alternative sequenceiVSP_004057164 – 251Missing in isoform I-mfC. 1 PublicationAdd BLAST88

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57820 mRNA Translation: AAC52791.1
U57821 mRNA Translation: AAC52792.1
U57822 mRNA Translation: AAC52793.1
BC006018 mRNA Translation: AAH06018.1
BC010259 mRNA Translation: AAH10259.1
BC083058 mRNA Translation: AAH83058.1
BC085233 mRNA Translation: AAH85233.1
CCDSiCCDS50134.1 [P70331-2]
RefSeqiNP_001103443.1, NM_001109973.2 [P70331-2]
NP_001263319.1, NM_001276390.1 [P70331-2]
NP_034913.2, NM_010783.3 [P70331-2]
XP_006523806.1, XM_006523743.3 [P70331-1]
XP_006523807.1, XM_006523744.3 [P70331-1]
XP_006523808.1, XM_006523745.3 [P70331-1]
UniGeneiMm.329100

Genome annotation databases

EnsembliENSMUST00000035375; ENSMUSP00000037888; ENSMUSG00000032717 [P70331-2]
ENSMUST00000066368; ENSMUSP00000069915; ENSMUSG00000032717 [P70331-2]
GeneIDi17240
KEGGimmu:17240
UCSCiuc008cwh.3 mouse [P70331-2]
uc008cwj.3 mouse [P70331-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57820 mRNA Translation: AAC52791.1
U57821 mRNA Translation: AAC52792.1
U57822 mRNA Translation: AAC52793.1
BC006018 mRNA Translation: AAH06018.1
BC010259 mRNA Translation: AAH10259.1
BC083058 mRNA Translation: AAH83058.1
BC085233 mRNA Translation: AAH85233.1
CCDSiCCDS50134.1 [P70331-2]
RefSeqiNP_001103443.1, NM_001109973.2 [P70331-2]
NP_001263319.1, NM_001276390.1 [P70331-2]
NP_034913.2, NM_010783.3 [P70331-2]
XP_006523806.1, XM_006523743.3 [P70331-1]
XP_006523807.1, XM_006523744.3 [P70331-1]
XP_006523808.1, XM_006523745.3 [P70331-1]
UniGeneiMm.329100

3D structure databases

ProteinModelPortaliP70331
SMRiP70331
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201368, 2 interactors
STRINGi10090.ENSMUSP00000037888

PTM databases

iPTMnetiP70331
PhosphoSitePlusiP70331

Proteomic databases

PaxDbiP70331
PRIDEiP70331

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035375; ENSMUSP00000037888; ENSMUSG00000032717 [P70331-2]
ENSMUST00000066368; ENSMUSP00000069915; ENSMUSG00000032717 [P70331-2]
GeneIDi17240
KEGGimmu:17240
UCSCiuc008cwh.3 mouse [P70331-2]
uc008cwj.3 mouse [P70331-1]

Organism-specific databases

CTDi4188
MGIiMGI:107687 Mdfi

Phylogenomic databases

eggNOGiENOG410IYH9 Eukaryota
ENOG410YK34 LUCA
GeneTreeiENSGT00390000010600
HOGENOMiHOG000001577
InParanoidiP70331
OrthoDBiEOG091G0RJH
PhylomeDBiP70331
TreeFamiTF332113

Miscellaneous databases

PROiPR:P70331
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032717 Expressed in 160 organ(s), highest expression level in cardiac ventricle
CleanExiMM_MDFI
ExpressionAtlasiP70331 baseline and differential
GenevisibleiP70331 MM

Family and domain databases

InterProiView protein in InterPro
IPR026134 MDFI/MDFIC
PANTHERiPTHR15304 PTHR15304, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMDFI_MOUSE
AccessioniPrimary (citable) accession number: P70331
Secondary accession number(s): P70330
, P70332, Q5XK64, Q99JM9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: February 1, 1997
Last modified: November 7, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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