UniProtKB - P70315 (WASP_MOUSE)
Protein
Wiskott-Aldrich syndrome protein homolog
Gene
Was
Organism
Mus musculus (Mouse)
Status
Functioni
Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria. In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. Promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).By similarity
GO - Molecular functioni
- actin binding Source: InterPro
- identical protein binding Source: MGI
- phospholipase binding Source: MGI
- protein kinase binding Source: MGI
- Rac GTPase binding Source: MGI
- SH3 domain binding Source: MGI
- small GTPase binding Source: MGI
GO - Biological processi
- actin filament-based movement Source: MGI
- actin filament polymerization Source: MGI
- actin polymerization or depolymerization Source: MGI
- Cdc42 protein signal transduction Source: MGI
- cellular response to interferon-gamma Source: MGI
- endosomal transport Source: MGI
- immune response Source: MGI
- negative regulation of cell motility Source: MGI
- negative regulation of stress fiber assembly Source: MGI
- positive regulation of Arp2/3 complex-mediated actin nucleation Source: InterPro
- positive regulation of double-strand break repair via homologous recombination Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- regulation of actin polymerization or depolymerization Source: MGI
- regulation of lamellipodium assembly Source: MGI
- regulation of stress fiber assembly Source: MGI
- regulation of T cell antigen processing and presentation Source: MGI
- T cell activation Source: MGI
Names & Taxonomyi
Protein namesi | Recommended name: Wiskott-Aldrich syndrome protein homologShort name: WASp |
Gene namesi | Name:Was Synonyms:Wasp |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:105059, Was |
Subcellular locationi
Cytoskeleton
- cytoskeleton By similarity
Nucleus
- Nucleus By similarity
Cytoskeleton
- actin filament Source: MGI
Cytosol
- cytosol Source: MGI
Nucleus
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- cell-cell junction Source: MGI
- phagocytic vesicle Source: MGI
- site of double-strand break Source: UniProtKB
- vesicle membrane Source: MGI
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000188991 | 1 – 520 | Wiskott-Aldrich syndrome protein homologAdd BLAST | 520 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 293 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 501 | PhosphoserineCombined sources | 1 | |
Modified residuei | 502 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Phosphorylated at Tyr-293 by FYN and HCK, inducing WAS effector activity after TCR engagement. Phosphorylation at Tyr-293 enhances WAS activity in promoting actin polymerization and filopodia formation.By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P70315 |
jPOSTi | P70315 |
PaxDbi | P70315 |
PeptideAtlasi | P70315 |
PRIDEi | P70315 |
PTM databases
iPTMneti | P70315 |
PhosphoSitePlusi | P70315 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000031165, Expressed in granulocyte and 114 other tissues |
ExpressionAtlasi | P70315, baseline and differential |
Genevisiblei | P70315, MM |
Interactioni
Subunit structurei
Binds the Arp2/3 complex (By similarity).
Interacts with CDC42, RAC, NCK, HCK, FYN, SRC kinase FGR, BTK, ABL1, PSTPIP1, WIP, and to the p85 subunit of PLC-gamma (PubMed:9488710).
Interacts (via C-terminus) with ALDOA (By similarity).
Interacts with NCK1 (via SH3 domains) (PubMed:8910519).
Interacts with FCHSD2 (PubMed:23437151).
By similarity3 PublicationsBinary interactionsi
Hide detailsP70315
With | #Exp. | IntAct |
---|---|---|
Ipo5 [Q8BKC5] | 8 | EBI-644195,EBI-643605 |
Src [P05480] | 2 | EBI-644195,EBI-298680 |
Wipf1 [Q8K1I7] | 3 | EBI-644195,EBI-644216 |
GO - Molecular functioni
- actin binding Source: InterPro
- identical protein binding Source: MGI
- phospholipase binding Source: MGI
- protein kinase binding Source: MGI
- Rac GTPase binding Source: MGI
- SH3 domain binding Source: MGI
- small GTPase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 204544, 10 interactors |
CORUMi | P70315 |
DIPi | DIP-36426N |
IntActi | P70315, 29 interactors |
MINTi | P70315 |
STRINGi | 10090.ENSMUSP00000033505 |
Miscellaneous databases
RNActi | P70315, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 41 – 150 | WH1PROSITE-ProRule annotationAdd BLAST | 110 | |
Domaini | 240 – 253 | CRIBPROSITE-ProRule annotationAdd BLAST | 14 | |
Repeati | 354 – 363 | GRSGPLPPXP motif 1 | 10 | |
Repeati | 393 – 402 | GRSGPLPPXP motif 2 | 10 | |
Domaini | 448 – 465 | WH2PROSITE-ProRule annotationAdd BLAST | 18 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 162 – 167 | Poly-Pro | 6 | |
Compositional biasi | 314 – 321 | Poly-Pro | 8 | |
Compositional biasi | 324 – 341 | Poly-GlyAdd BLAST | 18 | |
Compositional biasi | 368 – 373 | Poly-Pro | 6 | |
Compositional biasi | 376 – 379 | Poly-Pro | 4 | |
Compositional biasi | 384 – 390 | Poly-Pro | 7 | |
Compositional biasi | 397 – 403 | Poly-Pro | 7 | |
Compositional biasi | 408 – 424 | Poly-ProAdd BLAST | 17 | |
Compositional biasi | 503 – 520 | Asp/Glu-rich (acidic)Add BLAST | 18 |
Domaini
The WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.
The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG3671, Eukaryota |
GeneTreei | ENSGT00730000110895 |
HOGENOMi | CLU_015385_3_2_1 |
InParanoidi | P70315 |
OMAi | NRQEKKQ |
OrthoDBi | 1407277at2759 |
PhylomeDBi | P70315 |
TreeFami | TF316736 |
Family and domain databases
CDDi | cd01205, EVH1_WASP-like, 1 hit |
Gene3Di | 2.30.29.30, 1 hit 3.90.810.10, 2 hits |
InterProi | View protein in InterPro IPR000095, CRIB_dom IPR036936, CRIB_dom_sf IPR011993, PH-like_dom_sf IPR027641, WASP IPR011026, WASP_C IPR033927, WASPfam_EVH1 IPR000697, WH1/EVH1_dom IPR003124, WH2_dom |
PANTHERi | PTHR23202:SF79, PTHR23202:SF79, 2 hits |
Pfami | View protein in Pfam PF00786, PBD, 1 hit PF00568, WH1, 1 hit PF02205, WH2, 1 hit |
SMARTi | View protein in SMART SM00285, PBD, 1 hit SM00461, WH1, 1 hit SM00246, WH2, 1 hit |
SUPFAMi | SSF47912, SSF47912, 2 hits |
PROSITEi | View protein in PROSITE PS50108, CRIB, 1 hit PS50229, WH1, 1 hit PS51082, WH2, 1 hit |
i Sequence
Sequence statusi: Complete.
P70315-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNSGPGPVGG RPGGRGGPAV QQNIPSNLLQ DHENQRLFEL LGRKCWTLAT
60 70 80 90 100
TVVQLYLALP PGAEHWTMEH CGAVCFVKDN PQKSYFIRLY GLQAGRLLWE
110 120 130 140 150
QELYSQLVYL TPTPFFHTFA GDDCQVGLNF ADESEAQAFR ALVQEKIQKR
160 170 180 190 200
NQRQSGERRQ LPPPPAPINE ERRGGLPPVP PHPGGDHGGP SGGPLSLGLV
210 220 230 240 250
TVDIQNPDIT SSRYRGLPAP GPGPTDKKRS GKKKISKADI GAPSGFKHVS
260 270 280 290 300
HVGWDPQNGF DVNNLDPDLR SLFSRAGISE AQLTDAETSK LIYDFIEDQG
310 320 330 340 350
GLEAVRQEMR RQEPLPPPPP PCRGGGGGGG GGGGGGGGGG GQPLRPPVVG
360 370 380 390 400
SNKGRSGPLP PVPMGGAPPP PTPRGPPPPG RGGPPPPPPP ATGRSGPPPP
410 420 430 440 450
PLPGAGGPPA PPPPPPPPPP PPCPGSGPAP PPLPPTPVSG GSPAPGGGRG
460 470 480 490 500
ALLDQIRQGI QLNKTPGALE NSVQQPPAQQ SEGLVGALMH VMQKRSRVIH
510 520
SSDEGEDQTG EDEEDDEWDD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U54788 mRNA Translation: AAC52556.1 |
CCDSi | CCDS29984.1 |
RefSeqi | NP_033541.1, NM_009515.2 |
Genome annotation databases
Ensembli | ENSMUST00000033505; ENSMUSP00000033505; ENSMUSG00000031165 |
GeneIDi | 22376 |
KEGGi | mmu:22376 |
UCSCi | uc009sns.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U54788 mRNA Translation: AAC52556.1 |
CCDSi | CCDS29984.1 |
RefSeqi | NP_033541.1, NM_009515.2 |
3D structure databases
BMRBi | P70315 |
SMRi | P70315 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204544, 10 interactors |
CORUMi | P70315 |
DIPi | DIP-36426N |
IntActi | P70315, 29 interactors |
MINTi | P70315 |
STRINGi | 10090.ENSMUSP00000033505 |
PTM databases
iPTMneti | P70315 |
PhosphoSitePlusi | P70315 |
Proteomic databases
EPDi | P70315 |
jPOSTi | P70315 |
PaxDbi | P70315 |
PeptideAtlasi | P70315 |
PRIDEi | P70315 |
Protocols and materials databases
Antibodypediai | 701, 486 antibodies |
DNASUi | 22376 |
Genome annotation databases
Ensembli | ENSMUST00000033505; ENSMUSP00000033505; ENSMUSG00000031165 |
GeneIDi | 22376 |
KEGGi | mmu:22376 |
UCSCi | uc009sns.2, mouse |
Organism-specific databases
CTDi | 7454 |
MGIi | MGI:105059, Was |
Phylogenomic databases
eggNOGi | KOG3671, Eukaryota |
GeneTreei | ENSGT00730000110895 |
HOGENOMi | CLU_015385_3_2_1 |
InParanoidi | P70315 |
OMAi | NRQEKKQ |
OrthoDBi | 1407277at2759 |
PhylomeDBi | P70315 |
TreeFami | TF316736 |
Miscellaneous databases
BioGRID-ORCSi | 22376, 2 hits in 17 CRISPR screens |
PROi | PR:P70315 |
RNActi | P70315, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031165, Expressed in granulocyte and 114 other tissues |
ExpressionAtlasi | P70315, baseline and differential |
Genevisiblei | P70315, MM |
Family and domain databases
CDDi | cd01205, EVH1_WASP-like, 1 hit |
Gene3Di | 2.30.29.30, 1 hit 3.90.810.10, 2 hits |
InterProi | View protein in InterPro IPR000095, CRIB_dom IPR036936, CRIB_dom_sf IPR011993, PH-like_dom_sf IPR027641, WASP IPR011026, WASP_C IPR033927, WASPfam_EVH1 IPR000697, WH1/EVH1_dom IPR003124, WH2_dom |
PANTHERi | PTHR23202:SF79, PTHR23202:SF79, 2 hits |
Pfami | View protein in Pfam PF00786, PBD, 1 hit PF00568, WH1, 1 hit PF02205, WH2, 1 hit |
SMARTi | View protein in SMART SM00285, PBD, 1 hit SM00461, WH1, 1 hit SM00246, WH2, 1 hit |
SUPFAMi | SSF47912, SSF47912, 2 hits |
PROSITEi | View protein in PROSITE PS50108, CRIB, 1 hit PS50229, WH1, 1 hit PS51082, WH2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | WASP_MOUSE | |
Accessioni | P70315Primary (citable) accession number: P70315 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 175 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot