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Protein

Wiskott-Aldrich syndrome protein homolog

Gene

Was

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria. In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. Promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).By similarity

GO - Molecular functioni

  • actin filament binding Source: GO_Central
  • identical protein binding Source: MGI
  • phospholipase binding Source: MGI
  • protein kinase binding Source: MGI
  • Rac GTPase binding Source: MGI
  • SH3 domain binding Source: MGI
  • small GTPase binding Source: MGI

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-202433 Generation of second messenger molecules
R-MMU-2029482 Regulation of actin dynamics for phagocytic cup formation
R-MMU-5663213 RHO GTPases Activate WASPs and WAVEs

Names & Taxonomyi

Protein namesi
Recommended name:
Wiskott-Aldrich syndrome protein homolog
Short name:
WASp
Gene namesi
Name:Was
Synonyms:Wasp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:105059 Was

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001889911 – 520Wiskott-Aldrich syndrome protein homologAdd BLAST520

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei293PhosphotyrosineCombined sources1
Modified residuei501PhosphoserineCombined sources1
Modified residuei502PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated at Tyr-293 by FYN and HCK, inducing WAS effector activity after TCR engagement. Phosphorylation at Tyr-293 enhances WAS activity in promoting actin polymerization and filopodia formation.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP70315
PaxDbiP70315
PeptideAtlasiP70315
PRIDEiP70315

PTM databases

iPTMnetiP70315
PhosphoSitePlusiP70315

Expressioni

Gene expression databases

BgeeiENSMUSG00000031165 Expressed in 85 organ(s), highest expression level in bone marrow
CleanExiMM_WAS
ExpressionAtlasiP70315 baseline and differential
GenevisibleiP70315 MM

Interactioni

Subunit structurei

Binds the Arp2/3 complex (By similarity). Interacts with CDC42, RAC, NCK, HCK, FYN, SRC kinase FGR, BTK, ABL1, PSTPIP1, WIP, and to the p85 subunit of PLC-gamma (PubMed:9488710). Interacts (via C-terminus) with ALDOA (By similarity). Interacts with NCK1 (via SH3 domains) (PubMed:8910519).By similarity2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204544, 6 interactors
CORUMiP70315
DIPiDIP-36426N
IntActiP70315, 16 interactors
MINTiP70315
STRINGi10090.ENSMUSP00000033505

Structurei

3D structure databases

ProteinModelPortaliP70315
SMRiP70315
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 150WH1PROSITE-ProRule annotationAdd BLAST110
Domaini240 – 253CRIBPROSITE-ProRule annotationAdd BLAST14
Repeati354 – 363GRSGPLPPXP motif 110
Repeati393 – 402GRSGPLPPXP motif 210
Domaini448 – 465WH2PROSITE-ProRule annotationAdd BLAST18

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi162 – 167Poly-Pro6
Compositional biasi314 – 321Poly-Pro8
Compositional biasi324 – 341Poly-GlyAdd BLAST18
Compositional biasi368 – 373Poly-Pro6
Compositional biasi376 – 379Poly-Pro4
Compositional biasi384 – 390Poly-Pro7
Compositional biasi397 – 403Poly-Pro7
Compositional biasi408 – 424Poly-ProAdd BLAST17
Compositional biasi503 – 520Asp/Glu-rich (acidic)Add BLAST18

Domaini

The WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.
The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3671 Eukaryota
ENOG4111M1J LUCA
GeneTreeiENSGT00730000110895
HOVERGENiHBG000222
InParanoidiP70315
KOiK05747
OMAiFAADDCQ
OrthoDBiEOG091G0VCD
PhylomeDBiP70315
TreeFamiTF316736

Family and domain databases

CDDicd00132 CRIB, 1 hit
cd01205 EVH1_WASP-like, 1 hit
Gene3Di2.30.29.30, 1 hit
3.90.810.10, 2 hits
InterProiView protein in InterPro
IPR000095 CRIB_dom
IPR036936 CRIB_dom_sf
IPR011993 PH-like_dom_sf
IPR027641 WASP
IPR011026 WASP_C
IPR033927 WASPfam_EVH1
IPR000697 WH1/EVH1_dom
IPR003124 WH2_dom
PANTHERiPTHR23202:SF35 PTHR23202:SF35, 1 hit
PfamiView protein in Pfam
PF00786 PBD, 1 hit
PF00568 WH1, 1 hit
PF02205 WH2, 1 hit
SMARTiView protein in SMART
SM00285 PBD, 1 hit
SM00461 WH1, 1 hit
SM00246 WH2, 1 hit
SUPFAMiSSF47912 SSF47912, 2 hits
PROSITEiView protein in PROSITE
PS50108 CRIB, 1 hit
PS50229 WH1, 1 hit
PS51082 WH2, 1 hit

Sequencei

Sequence statusi: Complete.

P70315-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNSGPGPVGG RPGGRGGPAV QQNIPSNLLQ DHENQRLFEL LGRKCWTLAT
60 70 80 90 100
TVVQLYLALP PGAEHWTMEH CGAVCFVKDN PQKSYFIRLY GLQAGRLLWE
110 120 130 140 150
QELYSQLVYL TPTPFFHTFA GDDCQVGLNF ADESEAQAFR ALVQEKIQKR
160 170 180 190 200
NQRQSGERRQ LPPPPAPINE ERRGGLPPVP PHPGGDHGGP SGGPLSLGLV
210 220 230 240 250
TVDIQNPDIT SSRYRGLPAP GPGPTDKKRS GKKKISKADI GAPSGFKHVS
260 270 280 290 300
HVGWDPQNGF DVNNLDPDLR SLFSRAGISE AQLTDAETSK LIYDFIEDQG
310 320 330 340 350
GLEAVRQEMR RQEPLPPPPP PCRGGGGGGG GGGGGGGGGG GQPLRPPVVG
360 370 380 390 400
SNKGRSGPLP PVPMGGAPPP PTPRGPPPPG RGGPPPPPPP ATGRSGPPPP
410 420 430 440 450
PLPGAGGPPA PPPPPPPPPP PPCPGSGPAP PPLPPTPVSG GSPAPGGGRG
460 470 480 490 500
ALLDQIRQGI QLNKTPGALE NSVQQPPAQQ SEGLVGALMH VMQKRSRVIH
510 520
SSDEGEDQTG EDEEDDEWDD
Length:520
Mass (Da):54,192
Last modified:February 1, 1997 - v1
Checksum:i9C223733C59F0C8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54788 mRNA Translation: AAC52556.1
CCDSiCCDS29984.1
RefSeqiNP_033541.1, NM_009515.2
UniGeneiMm.4735

Genome annotation databases

EnsembliENSMUST00000033505; ENSMUSP00000033505; ENSMUSG00000031165
GeneIDi22376
KEGGimmu:22376
UCSCiuc009sns.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54788 mRNA Translation: AAC52556.1
CCDSiCCDS29984.1
RefSeqiNP_033541.1, NM_009515.2
UniGeneiMm.4735

3D structure databases

ProteinModelPortaliP70315
SMRiP70315
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204544, 6 interactors
CORUMiP70315
DIPiDIP-36426N
IntActiP70315, 16 interactors
MINTiP70315
STRINGi10090.ENSMUSP00000033505

PTM databases

iPTMnetiP70315
PhosphoSitePlusiP70315

Proteomic databases

EPDiP70315
PaxDbiP70315
PeptideAtlasiP70315
PRIDEiP70315

Protocols and materials databases

DNASUi22376
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033505; ENSMUSP00000033505; ENSMUSG00000031165
GeneIDi22376
KEGGimmu:22376
UCSCiuc009sns.2 mouse

Organism-specific databases

CTDi7454
MGIiMGI:105059 Was

Phylogenomic databases

eggNOGiKOG3671 Eukaryota
ENOG4111M1J LUCA
GeneTreeiENSGT00730000110895
HOVERGENiHBG000222
InParanoidiP70315
KOiK05747
OMAiFAADDCQ
OrthoDBiEOG091G0VCD
PhylomeDBiP70315
TreeFamiTF316736

Enzyme and pathway databases

ReactomeiR-MMU-202433 Generation of second messenger molecules
R-MMU-2029482 Regulation of actin dynamics for phagocytic cup formation
R-MMU-5663213 RHO GTPases Activate WASPs and WAVEs

Miscellaneous databases

PROiPR:P70315
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031165 Expressed in 85 organ(s), highest expression level in bone marrow
CleanExiMM_WAS
ExpressionAtlasiP70315 baseline and differential
GenevisibleiP70315 MM

Family and domain databases

CDDicd00132 CRIB, 1 hit
cd01205 EVH1_WASP-like, 1 hit
Gene3Di2.30.29.30, 1 hit
3.90.810.10, 2 hits
InterProiView protein in InterPro
IPR000095 CRIB_dom
IPR036936 CRIB_dom_sf
IPR011993 PH-like_dom_sf
IPR027641 WASP
IPR011026 WASP_C
IPR033927 WASPfam_EVH1
IPR000697 WH1/EVH1_dom
IPR003124 WH2_dom
PANTHERiPTHR23202:SF35 PTHR23202:SF35, 1 hit
PfamiView protein in Pfam
PF00786 PBD, 1 hit
PF00568 WH1, 1 hit
PF02205 WH2, 1 hit
SMARTiView protein in SMART
SM00285 PBD, 1 hit
SM00461 WH1, 1 hit
SM00246 WH2, 1 hit
SUPFAMiSSF47912 SSF47912, 2 hits
PROSITEiView protein in PROSITE
PS50108 CRIB, 1 hit
PS50229 WH1, 1 hit
PS51082 WH2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiWASP_MOUSE
AccessioniPrimary (citable) accession number: P70315
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 7, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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