Was

Functionⁱ

Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria. In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. Promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).By similarity

GO - Molecular functionⁱ

actin filament binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
identical protein binding
Source: MGI
Inferred from direct assayⁱ
- 12147689
phospholipase binding Source: MGI
protein kinase binding Source: MGI
Rac GTPase binding Source: MGI
SH3 domain binding Source: MGI
small GTPase binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

actin cortical patch assembly
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
actin cortical patch localization
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
actin filament-based movement
Source: MGI
Inferred from direct assayⁱ
- 12147689
actin filament polymerization
Source: MGI
Inferred from direct assayⁱ
- 12147689
actin polymerization or depolymerization
Source: MGI
Inferred from direct assayⁱ
- 12147689
Cdc42 protein signal transduction Source: MGI
cellular response to interferon-gamma
Source: MGI
Inferred from direct assayⁱ
- 19144319
endocytosis
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
endosomal transport
Source: MGI
Inferred from direct assayⁱ
- 12147689
immune response Source: MGI
negative regulation of cell motility Source: MGI
negative regulation of stress fiber assembly Source: MGI
positive regulation of actin nucleation
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
positive regulation of Arp2/3 complex-mediated actin nucleation Source: InterPro
positive regulation of double-strand break repair via homologous recombination Source: UniProtKB
positive regulation of transcription by RNA polymerase II Source: UniProtKB
regulation of actin polymerization or depolymerization Source: MGI
regulation of lamellipodium assembly Source: MGI
regulation of stress fiber assembly Source: MGI
regulation of T cell antigen processing and presentation Source: MGI
T cell activation
Source: MGI
Inferred from mutant phenotypeⁱ
- 11070165

View the complete GO annotation on QuickGO ...

Enzyme and pathway databases

Reactomeⁱ	R-MMU-202433 Generation of second messenger molecules R-MMU-2029482 Regulation of actin dynamics for phagocytic cup formation R-MMU-5663213 RHO GTPases Activate WASPs and WAVEs

Reactomeⁱ

R-MMU-202433 Generation of second messenger molecules
R-MMU-2029482 Regulation of actin dynamics for phagocytic cup formation
R-MMU-5663213 RHO GTPases Activate WASPs and WAVEs

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Wiskott-Aldrich syndrome protein homolog Short name: WASp
Gene namesⁱ	Name:Was Synonyms:Wasp
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome X

Organism-specific databases

MGIⁱ	MGI:105059 Was

Keywords - Cellular componentⁱ

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000188991	1 – 520	Wiskott-Aldrich syndrome protein homologAdd BLAST		520

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	293	PhosphotyrosineCombined sources	1
Modified residueⁱ	501	PhosphoserineCombined sources	1
Modified residueⁱ	502	PhosphoserineCombined sources	1

Post-translational modificationⁱ

Phosphorylated at Tyr-293 by FYN and HCK, inducing WAS effector activity after TCR engagement. Phosphorylation at Tyr-293 enhances WAS activity in promoting actin polymerization and filopodia formation.By similarity

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

EPDⁱ	P70315
PaxDbⁱ	P70315
PeptideAtlas More...PeptideAtlasⁱ	P70315
PRIDEⁱ	P70315

PTM databases

iPTMnetⁱ	P70315
PhosphoSitePlusⁱ	P70315

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000031165 Expressed in 85 organ(s), highest expression level in bone marrow
CleanExⁱ	MM_WAS
ExpressionAtlasⁱ	P70315 baseline and differential
Genevisibleⁱ	P70315 MM

Interactionⁱ

Subunit structureⁱ

Binds the Arp2/3 complex (By similarity). Interacts with CDC42, RAC, NCK, HCK, FYN, SRC kinase FGR, BTK, ABL1, PSTPIP1, WIP, and to the p85 subunit of PLC-gamma (PubMed:9488710). Interacts (via C-terminus) with ALDOA (By similarity). Interacts with NCK1 (via SH3 domains) (PubMed:8910519).By similarity2 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
Ipo5	Q8BKC5	8	EBI-644195,EBI-643605
Src	P05480	2	EBI-644195,EBI-298680
Wipf1	Q8K1I7	3	EBI-644195,EBI-644216

GO - Molecular functionⁱ

actin filament binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
identical protein binding
Source: MGI
Inferred from direct assayⁱ
- 12147689
phospholipase binding Source: MGI
protein kinase binding Source: MGI
Rac GTPase binding Source: MGI
SH3 domain binding Source: MGI
small GTPase binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	204544, 6 interactors
CORUMⁱ	P70315
Database of interacting proteins More...DIPⁱ	DIP-36426N
IntActⁱ	P70315, 16 interactors
Molecular INTeraction database More...MINTⁱ	P70315
STRINGⁱ	10090.ENSMUSP00000033505

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P70315
SMRⁱ	P70315
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	41 – 150	WH1PROSITE-ProRule annotationAdd BLAST	110
Domainⁱ	240 – 253	CRIBPROSITE-ProRule annotationAdd BLAST	14
Repeatⁱ	354 – 363	GRSGPLPPXP motif 1	10
Repeatⁱ	393 – 402	GRSGPLPPXP motif 2	10
Domainⁱ	448 – 465	WH2PROSITE-ProRule annotationAdd BLAST	18

Compositional bias

Feature key	Position(s)	DescriptionActions	Length
Compositional biasⁱ	162 – 167	Poly-Pro	6
Compositional biasⁱ	314 – 321	Poly-Pro	8
Compositional biasⁱ	324 – 341	Poly-GlyAdd BLAST	18
Compositional biasⁱ	368 – 373	Poly-Pro	6
Compositional biasⁱ	376 – 379	Poly-Pro	4
Compositional biasⁱ	384 – 390	Poly-Pro	7
Compositional biasⁱ	397 – 403	Poly-Pro	7
Compositional biasⁱ	408 – 424	Poly-ProAdd BLAST	17
Compositional biasⁱ	503 – 520	Asp/Glu-rich (acidic)Add BLAST	18

Domainⁱ

The WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.

The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.

Keywords - Domainⁱ

Repeat

Phylogenomic databases

eggNOGⁱ	KOG3671 Eukaryota ENOG4111M1J LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00730000110895
HOVERGENⁱ	HBG000222
InParanoidⁱ	P70315
KEGG Orthology (KO) More...KOⁱ	K05747
OMAⁱ	FAADDCQ
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0VCD
PhylomeDBⁱ	P70315
TreeFamⁱ	TF316736

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00132 CRIB, 1 hit cd01205 EVH1_WASP-like, 1 hit
Gene3Dⁱ	2.30.29.30, 1 hit 3.90.810.10, 2 hits
InterProⁱ	View protein in InterPro IPR000095 CRIB_dom IPR036936 CRIB_dom_sf IPR011993 PH-like_dom_sf IPR027641 WASP IPR011026 WASP_C IPR033927 WASPfam_EVH1 IPR000697 WH1/EVH1_dom IPR003124 WH2_dom
PANTHERⁱ	PTHR23202:SF35 PTHR23202:SF35, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00786 PBD, 1 hit PF00568 WH1, 1 hit PF02205 WH2, 1 hit
SMARTⁱ	View protein in SMART SM00285 PBD, 1 hit SM00461 WH1, 1 hit SM00246 WH2, 1 hit
SUPFAMⁱ	SSF47912 SSF47912, 2 hits
PROSITEⁱ	View protein in PROSITE PS50108 CRIB, 1 hit PS50229 WH1, 1 hit PS51082 WH2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P70315-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MNSGPGPVGG RPGGRGGPAV QQNIPSNLLQ DHENQRLFEL LGRKCWTLAT 
        60         70         80         90        100
TVVQLYLALP PGAEHWTMEH CGAVCFVKDN PQKSYFIRLY GLQAGRLLWE 
       110        120        130        140        150
QELYSQLVYL TPTPFFHTFA GDDCQVGLNF ADESEAQAFR ALVQEKIQKR 
       160        170        180        190        200
NQRQSGERRQ LPPPPAPINE ERRGGLPPVP PHPGGDHGGP SGGPLSLGLV 
       210        220        230        240        250
TVDIQNPDIT SSRYRGLPAP GPGPTDKKRS GKKKISKADI GAPSGFKHVS 
       260        270        280        290        300
HVGWDPQNGF DVNNLDPDLR SLFSRAGISE AQLTDAETSK LIYDFIEDQG 
       310        320        330        340        350
GLEAVRQEMR RQEPLPPPPP PCRGGGGGGG GGGGGGGGGG GQPLRPPVVG 
       360        370        380        390        400
SNKGRSGPLP PVPMGGAPPP PTPRGPPPPG RGGPPPPPPP ATGRSGPPPP 
       410        420        430        440        450
PLPGAGGPPA PPPPPPPPPP PPCPGSGPAP PPLPPTPVSG GSPAPGGGRG 
       460        470        480        490        500
ALLDQIRQGI QLNKTPGALE NSVQQPPAQQ SEGLVGALMH VMQKRSRVIH 
       510        520
SSDEGEDQTG EDEEDDEWDD

Length:520

Mass (Da):54,192

Last modified:February 1, 1997 - v1

Checksum:ⁱ9C223733C59F0C8A

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U54788 mRNA Translation: AAC52556.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS29984.1
NCBI Reference Sequences More...RefSeqⁱ	NP_033541.1, NM_009515.2
UniGeneⁱ	Mm.4735

Genome annotation databases

Ensemblⁱ	ENSMUST00000033505; ENSMUSP00000033505; ENSMUSG00000031165
GeneIDⁱ	22376
KEGGⁱ	mmu:22376
UCSC genome browser More...UCSCⁱ	uc009sns.2 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P70315	Wiskott-Aldrich syndrome protein (WASP)		MOUSE		520	UniRef100_P70315

Protein	Similar proteins		Species	Score	Length	Source
P70315	Wiskott-Aldrich syndrome protein (WASP)		MOUSE		520	UniRef90_P70315
wiskott-Aldrich syndrome protein		MESAU		515
Wiskott-Aldrich syndrome		RAT		503
UPI00042AB29E		PERMB		516
UPI000A30EE5B		MUSPA		511
+2

Protein	Similar proteins		Species	Score	Length	Source
P70315	Wiskott-Aldrich syndrome protein (WASP)		MOUSE		520	UniRef50_P70315
wiskott-Aldrich syndrome protein		MESAU		515
Was protein		RAT		503
Wiskott-Aldrich syndrome protein like protein		TUPCH		484
Uncharacterized protein		MONDO		504
+90

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U54788 mRNA Translation: AAC52556.1
CCDSⁱ	CCDS29984.1
RefSeqⁱ	NP_033541.1, NM_009515.2
UniGeneⁱ	Mm.4735

3D structure databases

ProteinModelPortalⁱ	P70315
SMRⁱ	P70315
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	204544, 6 interactors
CORUMⁱ	P70315
DIPⁱ	DIP-36426N
IntActⁱ	P70315, 16 interactors
MINTⁱ	P70315
STRINGⁱ	10090.ENSMUSP00000033505

PTM databases

iPTMnetⁱ	P70315
PhosphoSitePlusⁱ	P70315

Proteomic databases

EPDⁱ	P70315
PaxDbⁱ	P70315
PeptideAtlasⁱ	P70315
PRIDEⁱ	P70315

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	22376
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000033505; ENSMUSP00000033505; ENSMUSG00000031165
GeneIDⁱ	22376
KEGGⁱ	mmu:22376
UCSCⁱ	uc009sns.2 mouse

Organism-specific databases

CTDⁱ	7454
MGIⁱ	MGI:105059 Was

Phylogenomic databases

eggNOGⁱ	KOG3671 Eukaryota ENOG4111M1J LUCA
GeneTreeⁱ	ENSGT00730000110895
HOVERGENⁱ	HBG000222
InParanoidⁱ	P70315
KOⁱ	K05747
OMAⁱ	FAADDCQ
OrthoDBⁱ	EOG091G0VCD
PhylomeDBⁱ	P70315
TreeFamⁱ	TF316736

Enzyme and pathway databases

Reactomeⁱ	R-MMU-202433 Generation of second messenger molecules R-MMU-2029482 Regulation of actin dynamics for phagocytic cup formation R-MMU-5663213 RHO GTPases Activate WASPs and WAVEs

Reactomeⁱ

R-MMU-202433 Generation of second messenger molecules
R-MMU-2029482 Regulation of actin dynamics for phagocytic cup formation
R-MMU-5663213 RHO GTPases Activate WASPs and WAVEs

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P70315
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000031165 Expressed in 85 organ(s), highest expression level in bone marrow
CleanExⁱ	MM_WAS
ExpressionAtlasⁱ	P70315 baseline and differential
Genevisibleⁱ	P70315 MM

Family and domain databases

CDDⁱ	cd00132 CRIB, 1 hit cd01205 EVH1_WASP-like, 1 hit
Gene3Dⁱ	2.30.29.30, 1 hit 3.90.810.10, 2 hits
InterProⁱ	View protein in InterPro IPR000095 CRIB_dom IPR036936 CRIB_dom_sf IPR011993 PH-like_dom_sf IPR027641 WASP IPR011026 WASP_C IPR033927 WASPfam_EVH1 IPR000697 WH1/EVH1_dom IPR003124 WH2_dom
PANTHERⁱ	PTHR23202:SF35 PTHR23202:SF35, 1 hit
Pfamⁱ	View protein in Pfam PF00786 PBD, 1 hit PF00568 WH1, 1 hit PF02205 WH2, 1 hit
SMARTⁱ	View protein in SMART SM00285 PBD, 1 hit SM00461 WH1, 1 hit SM00246 WH2, 1 hit
SUPFAMⁱ	SSF47912 SSF47912, 2 hits
PROSITEⁱ	View protein in PROSITE PS50108 CRIB, 1 hit PS50229 WH1, 1 hit PS51082 WH2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	WASP_MOUSE
Accessionⁱ	P70315Primary (citable) accession number: P70315
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
	Last sequence update:	February 1, 1997
	Last modified:	November 7, 2018
	This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P70315 (WASP_MOUSE)

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Wiskott-Aldrich syndrome protein homolog

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Cytoskeleton

Cytoskeleton

Cytosol

Nucleus

Other locations

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Compositional bias

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ