UniProtKB - P70290 (EM55_MOUSE)
Protein
55 kDa erythrocyte membrane protein
Gene
Mpp1
Organism
Mus musculus (Mouse)
Status
Functioni
Essential regulator of neutrophil polarity. Regulates neutrophil polarization by regulating AKT1 phosphorylation through a mechanism that is independent of PIK3CG activity.1 Publication
GO - Biological processi
- regulation of neutrophil chemotaxis Source: UniProtKB
Names & Taxonomyi
Protein namesi | Recommended name: 55 kDa erythrocyte membrane proteinShort name: p55 Alternative name(s): Membrane protein, palmitoylated 1 |
Gene namesi | Name:Mpp1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:105941, Mpp1 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Lipid-anchor By similarity
Other locations
- stereocilium 2 Publications
Note: Colocalizes with WHRN at stereocilium tip during hair cell development (PubMed:16829577). Colocalizes with MPP5 in the retina, at the outer limiting membrane (OLM) (PubMed:17584769). Colocalizes with WHRN in the retina, at the outer limiting membrane (OLM), outer plexifirm layer (OPL), basal bodies, and at connecting cilium (CC) (PubMed:17584769). Colocalizes with NF2 in non-myelin-forming Schwann cells (By similarity).By similarity2 Publications
Cytoskeleton
- centriolar satellite Source: MGI
- cortical cytoskeleton Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- membrane Source: MGI
- stereocilium Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, Cell projection, MembranePathology & Biotechi
Disruption phenotypei
Neutrophils form multiple transient pseudopods upon chemotactic stimulation, and do not migrate efficiently in vitro.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000094566 | 2 – 466 | 55 kDa erythrocyte membrane proteinAdd BLAST | 465 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylthreonineBy similarity | 1 | |
Modified residuei | 13 | PhosphoserineBy similarity | 1 | |
Modified residuei | 19 | PhosphoserineBy similarity | 1 | |
Modified residuei | 49 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 52 | PhosphoserineCombined sources | 1 | |
Modified residuei | 57 | PhosphoserineCombined sources | 1 | |
Modified residuei | 110 | PhosphoserineCombined sources | 1 | |
Modified residuei | 243 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Palmitoylated.By similarity
Keywords - PTMi
Acetylation, Lipoprotein, Palmitate, PhosphoproteinProteomic databases
EPDi | P70290 |
jPOSTi | P70290 |
PaxDbi | P70290 |
PeptideAtlasi | P70290 |
PRIDEi | P70290 |
PTM databases
iPTMneti | P70290 |
PhosphoSitePlusi | P70290 |
SwissPalmi | P70290 |
Expressioni
Tissue specificityi
Abundantly expressed in the erythrocytes (at protein level).1 Publication
Developmental stagei
During development of the retina, expression is highest in the inner, neuroblastic layer, while at later stages it is also present in the photoreceptor cell layer. Detected in the eye from 14.5 dpc onwards. Highly expressed in the liver and primitive gut, and at lower levels in the umbilical vein, the ventricular layer of the CNS and upper/lower jaw region. At 14.5 dpc and 16.5 dpc, expression in the liver and stomach is maintained. In addition, expression is present in bone structures (e.g. zygomatic bone, lower jawbone), cranial nerve ganglia [e.g. trigeminal (V) ganglion] and cochlea. The expression in the eye is seen in the neuroblastic layer. At 16.5 dpc and 18.5 dpc, a slightly higher expression is seen in the neuroblastic layer. At 16.5 dpc, strong expression in the upper part of the gut is maintained and with the onset of ossification, expression is seen in all bone structures of the body. At P7 and P90, expression in the eye is seen in the ganglion cell layer, the inner nuclear layer (INL) and photoreceptor cell layer.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000031402, Expressed in blood and 309 other tissues |
ExpressionAtlasi | P70290, baseline and differential |
Genevisiblei | P70290, MM |
Interactioni
Subunit structurei
Heterodimer with MPP5.
Interacts with DLG5 and NF2.
Interacts (via guanylate kinase-like domain) with WHRN (via third PDZ domain).
2 PublicationsBinary interactionsi
P70290
With | #Exp. | IntAct |
---|---|---|
Whrn [Q80VW5] | 4 | EBI-8315951,EBI-7417603 |
Protein-protein interaction databases
BioGRIDi | 201480, 3 interactors |
DIPi | DIP-61219N |
IntActi | P70290, 5 interactors |
MINTi | P70290 |
STRINGi | 10090.ENSMUSP00000033775 |
Miscellaneous databases
RNActi | P70290, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 71 – 152 | PDZPROSITE-ProRule annotationAdd BLAST | 82 | |
Domaini | 158 – 228 | SH3PROSITE-ProRule annotationAdd BLAST | 71 | |
Domaini | 282 – 451 | Guanylate kinase-likePROSITE-ProRule annotationAdd BLAST | 170 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 268 – 466 | Interaction with MPP5By similarityAdd BLAST | 199 |
Sequence similaritiesi
Belongs to the MAGUK family.Curated
Keywords - Domaini
SH3 domainPhylogenomic databases
eggNOGi | KOG0609, Eukaryota |
GeneTreei | ENSGT00940000158744 |
HOGENOMi | CLU_001715_5_1_1 |
InParanoidi | P70290 |
OMAi | AMTEDIY |
OrthoDBi | 95102at2759 |
PhylomeDBi | P70290 |
TreeFami | TF314263 |
Family and domain databases
CDDi | cd12080, SH3_MPP1, 1 hit |
Gene3Di | 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR008145, GK/Ca_channel_bsu IPR008144, Guanylate_kin-like_dom IPR020590, Guanylate_kinase_CS IPR035475, MPP1_SH3 IPR027417, P-loop_NTPase IPR001478, PDZ IPR036034, PDZ_sf IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
Pfami | View protein in Pfam PF00625, Guanylate_kin, 1 hit PF00595, PDZ, 1 hit PF00018, SH3_1, 1 hit |
SMARTi | View protein in SMART SM00072, GuKc, 1 hit SM00228, PDZ, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit SSF50156, SSF50156, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00856, GUANYLATE_KINASE_1, 1 hit PS50052, GUANYLATE_KINASE_2, 1 hit PS50106, PDZ, 1 hit PS50002, SH3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All
P70290-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTLKSSEGEG GNSMRTALSD LYLEHLLQKR NRPETSLNQS NVTTEDMYTN
60 70 80 90 100
GSPAPGSPAH AKGQEARRVR LIQFEKITEE PMGITLKLNE KQSCTVARIL
110 120 130 140 150
HGGMIHRQGS LHVGDEILEI NGTNVTNHSV DQLQKAMKET KGMISLKVIA
160 170 180 190 200
NQQSRLPALQ MFMRAQFDYD PQKDNLIPCK EAGLKFVTGD IIQIINKDDS
210 220 230 240 250
NWWQGRVEGS SKESAGLIPS PELQEWRVAS VAHSAPSEAP SCSPFGKKKK
260 270 280 290 300
CKDKYLAKHS SIFDQLDVVS YEEVVRLPAF KRKTLVLIGA SGVGRSHIKN
310 320 330 340 350
GLLSHNPEKF AYPAPYTTRP PKKSEEDGKE YHFISTEEMT KNISANEFLE
360 370 380 390 400
FGSYQGNMFG TKFETVHQIH KQDKIAILDI EPQTLKTVRT AELSPFIVFI
410 420 430 440 450
APTDQGTQTE ALQQLQKDSE AIRSQYAHYF DLSLVNNSVD ETLKKLQEAF
460
DQACSSPQWV PVSWVY
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2AN84 | A2AN84_MOUSE | 55 kDa erythrocyte membrane protein | Mpp1 | 452 | Annotation score: | ||
B7ZCL8 | B7ZCL8_MOUSE | 55 kDa erythrocyte membrane protein | Mpp1 | 446 | Annotation score: | ||
B7ZCL9 | B7ZCL9_MOUSE | 55 kDa erythrocyte membrane protein | Mpp1 | 205 | Annotation score: | ||
B7ZCM0 | B7ZCM0_MOUSE | 55 kDa erythrocyte membrane protein | Mpp1 | 156 | Annotation score: | ||
B7ZCM1 | B7ZCM1_MOUSE | 55 kDa erythrocyte membrane protein | Mpp1 | 130 | Annotation score: | ||
D6RFD5 | D6RFD5_MOUSE | 55 kDa erythrocyte membrane protein | Mpp1 | 45 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U38196 mRNA Translation: AAC52970.1 BC013444 mRNA Translation: AAH13444.1 |
CCDSi | CCDS30236.1 |
RefSeqi | NP_032647.1, NM_008621.3 |
Genome annotation databases
Ensembli | ENSMUST00000033775; ENSMUSP00000033775; ENSMUSG00000031402 |
GeneIDi | 17524 |
KEGGi | mmu:17524 |
UCSCi | uc009tpp.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U38196 mRNA Translation: AAC52970.1 BC013444 mRNA Translation: AAH13444.1 |
CCDSi | CCDS30236.1 |
RefSeqi | NP_032647.1, NM_008621.3 |
3D structure databases
SMRi | P70290 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201480, 3 interactors |
DIPi | DIP-61219N |
IntActi | P70290, 5 interactors |
MINTi | P70290 |
STRINGi | 10090.ENSMUSP00000033775 |
PTM databases
iPTMneti | P70290 |
PhosphoSitePlusi | P70290 |
SwissPalmi | P70290 |
Proteomic databases
EPDi | P70290 |
jPOSTi | P70290 |
PaxDbi | P70290 |
PeptideAtlasi | P70290 |
PRIDEi | P70290 |
Protocols and materials databases
Antibodypediai | 419, 326 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000033775; ENSMUSP00000033775; ENSMUSG00000031402 |
GeneIDi | 17524 |
KEGGi | mmu:17524 |
UCSCi | uc009tpp.1, mouse |
Organism-specific databases
CTDi | 4354 |
MGIi | MGI:105941, Mpp1 |
Phylogenomic databases
eggNOGi | KOG0609, Eukaryota |
GeneTreei | ENSGT00940000158744 |
HOGENOMi | CLU_001715_5_1_1 |
InParanoidi | P70290 |
OMAi | AMTEDIY |
OrthoDBi | 95102at2759 |
PhylomeDBi | P70290 |
TreeFami | TF314263 |
Miscellaneous databases
BioGRID-ORCSi | 17524, 1 hit in 17 CRISPR screens |
ChiTaRSi | Mpp1, mouse |
PROi | PR:P70290 |
RNActi | P70290, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031402, Expressed in blood and 309 other tissues |
ExpressionAtlasi | P70290, baseline and differential |
Genevisiblei | P70290, MM |
Family and domain databases
CDDi | cd12080, SH3_MPP1, 1 hit |
Gene3Di | 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR008145, GK/Ca_channel_bsu IPR008144, Guanylate_kin-like_dom IPR020590, Guanylate_kinase_CS IPR035475, MPP1_SH3 IPR027417, P-loop_NTPase IPR001478, PDZ IPR036034, PDZ_sf IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
Pfami | View protein in Pfam PF00625, Guanylate_kin, 1 hit PF00595, PDZ, 1 hit PF00018, SH3_1, 1 hit |
SMARTi | View protein in SMART SM00072, GuKc, 1 hit SM00228, PDZ, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit SSF50156, SSF50156, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00856, GUANYLATE_KINASE_1, 1 hit PS50052, GUANYLATE_KINASE_2, 1 hit PS50106, PDZ, 1 hit PS50002, SH3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EM55_MOUSE | |
Accessioni | P70290Primary (citable) accession number: P70290 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 171 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families