Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nuclear factor 1 X-type

Gene

Nfix

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Isoform NFIX1 acts as a transcriptional activator while isoform NFIX3 acts as a repressor.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi1 – 194CTF/NF-IPROSITE-ProRule annotationAdd BLAST194

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processDNA replication, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear factor 1 X-type
Short name:
NF1-X
Short name:
Nuclear factor 1/X
Alternative name(s):
CCAAT-box-binding transcription factor
Short name:
CTF
Nuclear factor I/X
Short name:
NF-I/X
Short name:
NFI-X
TGGCA-binding protein
Gene namesi
Name:Nfix
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:97311 Nfix

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001002051 – 488Nuclear factor 1 X-typeAdd BLAST488

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei265PhosphoserineCombined sources1
Cross-linki279Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei280PhosphoserineCombined sources1
Modified residuei288PhosphoserineCombined sources1
Modified residuei301PhosphoserineCombined sources1
Modified residuei341PhosphoserineCombined sources1
Modified residuei343Asymmetric dimethylarginineCombined sources1
Modified residuei390Asymmetric dimethylarginineCombined sources1
Isoform NFIX2 (identifier: P70257-2)
Modified residuei301PhosphoserineCombined sources1
Modified residuei320PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP70257
PeptideAtlasiP70257
PRIDEiP70257

PTM databases

iPTMnetiP70257
PhosphoSitePlusiP70257

Expressioni

Gene expression databases

BgeeiENSMUSG00000001911 Expressed in 302 organ(s), highest expression level in rostral migratory stream
CleanExiMM_NFIX
ExpressionAtlasiP70257 baseline and differential
GenevisibleiP70257 MM

Interactioni

Subunit structurei

Binds DNA as a homodimer.

Binary interactionsi

Protein-protein interaction databases

BioGridi201750, 1 interactor
IntActiP70257, 5 interactors
MINTiP70257
STRINGi10090.ENSMUSP00000105386

Structurei

3D structure databases

ProteinModelPortaliP70257
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CTF/NF-I family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3663 Eukaryota
ENOG410Y16G LUCA
GeneTreeiENSGT00390000009905
HOGENOMiHOG000013028
HOVERGENiHBG006561
InParanoidiP70257
KOiK09171
PhylomeDBiP70257

Family and domain databases

InterProiView protein in InterPro
IPR000647 CTF/NFI
IPR020604 CTF/NFI_DNA-bd-dom
IPR019739 CTF/NFI_DNA-bd_CS
IPR019548 CTF/NFI_DNA-bd_N
IPR003619 MAD_homology1_Dwarfin-type
PANTHERiPTHR11492 PTHR11492, 1 hit
PfamiView protein in Pfam
PF00859 CTF_NFI, 1 hit
PF03165 MH1, 1 hit
PF10524 NfI_DNAbd_pre-N, 1 hit
SMARTiView protein in SMART
SM00523 DWA, 1 hit
PROSITEiView protein in PROSITE
PS00349 CTF_NFI_1, 1 hit
PS51080 CTF_NFI_2, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform NFIX3 (identifier: P70257-3) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MYSPYCLTQD EFHPFIEALL PHVRAFSYTW FNLQARKRKY FKKHEKRMSK
60 70 80 90 100
DEERAVKDEL LGEKPEIKQK WASRLLAKLR KDIRPEFRED FVLTITGKKP
110 120 130 140 150
PCCVLSNPDQ KGKIRRIDCL RQADKVWRLD LVMVILFKGI PLESTDGERL
160 170 180 190 200
YKSPQCSNPG LCVQPHHIGV TIKELDLYLA YFVHTPESGQ SDSSNQQGDA
210 220 230 240 250
DIKPLPNGHL SFQDCFVTSG VWNVTELVRV SQTPVATASG PNFSLADLES
260 270 280 290 300
PSYYNINQVT LGRRSITSPP STSSTKRPKS IDDSEMESPV DDVFYPGTGR
310 320 330 340 350
SPAAGSSQSS GWPNDVDAGP ASLKKSGKLD FCSALSSQGS SPRMAFTHHP
360 370 380 390 400
LPVLAGVRPG SPRATASALH FPSTSIIQQS SPYFTHPTIR YHHHHGQDSL
410 420 430 440 450
KEFVQFVCSD GSGQATGQPN GSGQGKVPGS FLLPPPPPVA RPVPLPMPDS
460 470 480
KTTSTAPDGA ALTPPSPSFT TTGASSANRF VGIGPRDG
Length:488
Mass (Da):53,394
Last modified:June 16, 2003 - v2
Checksum:i157F67EC17C96AD0
GO
Isoform NFIX1 (identifier: P70257-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     419-441: PNGSGQGKVPGSFLLPPPPPVAR → HSQRQAPPLPTGLSASDPGTATF
     442-488: Missing.

Show »
Length:441
Mass (Da):48,853
Checksum:iEB46ABAD96CE9E80
GO
Isoform NFIX2 (identifier: P70257-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     320-360: Missing.
     419-441: PNGSGQGKVPGSFLLPPPPPVAR → HSQRQAPPLPTGLSASDPGTATF
     442-488: Missing.

Show »
Length:400
Mass (Da):44,637
Checksum:i4E89D0236A3F7235
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PUH7E9PUH7_MOUSE
Nuclear factor 1
Nfix
494Annotation score:
Q3TYK3Q3TYK3_MOUSE
Nuclear factor 1
Nfix
391Annotation score:
D3YZ00D3YZ00_MOUSE
Nuclear factor 1
Nfix
488Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti353V → L in CAA68952 (PubMed:12568726).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003563320 – 360Missing in isoform NFIX2. 3 PublicationsAdd BLAST41
Alternative sequenceiVSP_007546419 – 441PNGSG…PPVAR → HSQRQAPPLPTGLSASDPGT ATF in isoform NFIX1 and isoform NFIX2. 4 PublicationsAdd BLAST23
Alternative sequenceiVSP_007547442 – 488Missing in isoform NFIX1 and isoform NFIX2. 4 PublicationsAdd BLAST47

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S81451 mRNA Translation: AAB36083.2
Y07688 mRNA Translation: CAA68952.1
Y07689 mRNA Translation: CAA68953.1
BC003766 mRNA Translation: AAH03766.1
U57636 mRNA Translation: AAB49931.1
AF111266 Genomic DNA Translation: AAD39101.1
CCDSiCCDS40412.1 [P70257-2]
CCDS40413.1 [P70257-1]
RefSeqiNP_001075451.1, NM_001081982.2 [P70257-1]
NP_035036.1, NM_010906.3 [P70257-2]
UniGeneiMm.487058
Mm.9394

Genome annotation databases

EnsembliENSMUST00000076715; ENSMUSP00000076005; ENSMUSG00000001911 [P70257-2]
ENSMUST00000099070; ENSMUSP00000096669; ENSMUSG00000001911 [P70257-1]
GeneIDi18032
KEGGimmu:18032
UCSCiuc009mne.2 mouse [P70257-1]
uc009mnf.2 mouse [P70257-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S81451 mRNA Translation: AAB36083.2
Y07688 mRNA Translation: CAA68952.1
Y07689 mRNA Translation: CAA68953.1
BC003766 mRNA Translation: AAH03766.1
U57636 mRNA Translation: AAB49931.1
AF111266 Genomic DNA Translation: AAD39101.1
CCDSiCCDS40412.1 [P70257-2]
CCDS40413.1 [P70257-1]
RefSeqiNP_001075451.1, NM_001081982.2 [P70257-1]
NP_035036.1, NM_010906.3 [P70257-2]
UniGeneiMm.487058
Mm.9394

3D structure databases

ProteinModelPortaliP70257
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201750, 1 interactor
IntActiP70257, 5 interactors
MINTiP70257
STRINGi10090.ENSMUSP00000105386

PTM databases

iPTMnetiP70257
PhosphoSitePlusiP70257

Proteomic databases

PaxDbiP70257
PeptideAtlasiP70257
PRIDEiP70257

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000076715; ENSMUSP00000076005; ENSMUSG00000001911 [P70257-2]
ENSMUST00000099070; ENSMUSP00000096669; ENSMUSG00000001911 [P70257-1]
GeneIDi18032
KEGGimmu:18032
UCSCiuc009mne.2 mouse [P70257-1]
uc009mnf.2 mouse [P70257-2]

Organism-specific databases

CTDi4784
MGIiMGI:97311 Nfix

Phylogenomic databases

eggNOGiKOG3663 Eukaryota
ENOG410Y16G LUCA
GeneTreeiENSGT00390000009905
HOGENOMiHOG000013028
HOVERGENiHBG006561
InParanoidiP70257
KOiK09171
PhylomeDBiP70257

Miscellaneous databases

PROiPR:P70257
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000001911 Expressed in 302 organ(s), highest expression level in rostral migratory stream
CleanExiMM_NFIX
ExpressionAtlasiP70257 baseline and differential
GenevisibleiP70257 MM

Family and domain databases

InterProiView protein in InterPro
IPR000647 CTF/NFI
IPR020604 CTF/NFI_DNA-bd-dom
IPR019739 CTF/NFI_DNA-bd_CS
IPR019548 CTF/NFI_DNA-bd_N
IPR003619 MAD_homology1_Dwarfin-type
PANTHERiPTHR11492 PTHR11492, 1 hit
PfamiView protein in Pfam
PF00859 CTF_NFI, 1 hit
PF03165 MH1, 1 hit
PF10524 NfI_DNAbd_pre-N, 1 hit
SMARTiView protein in SMART
SM00523 DWA, 1 hit
PROSITEiView protein in PROSITE
PS00349 CTF_NFI_1, 1 hit
PS51080 CTF_NFI_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNFIX_MOUSE
AccessioniPrimary (citable) accession number: P70257
Secondary accession number(s): O08519
, P70258, Q64192, Q99L78, Q9R1G2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 16, 2003
Last modified: November 7, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again