UniProtKB - P70208 (PLXA3_MOUSE)
Plexin-A3
Plxna3
Functioni
Coreceptor for SEMA3A and SEMA3F. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance in the developing nervous system. Regulates the migration of sympathetic neurons, but not of neural crest precursors. Required for normal dendrite spine morphology in pyramidal neurons. May play a role in regulating semaphorin-mediated programmed cell death in the developing nervous system. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm.
6 PublicationsGO - Molecular functioni
- semaphorin receptor activity Source: UniProtKB
GO - Biological processi
- axon guidance Source: MGI
- branchiomotor neuron axon guidance Source: ParkinsonsUK-UCL
- facial nerve structural organization Source: ParkinsonsUK-UCL
- hippocampus development Source: MGI
- negative chemotaxis Source: MGI
- negative regulation of axon extension involved in axon guidance Source: MGI
- negative regulation of cell adhesion Source: GO_Central
- neuron projection extension Source: MGI
- neuron projection guidance Source: MGI
- positive regulation of axonogenesis Source: GO_Central
- positive regulation of cytoskeleton organization Source: UniProtKB
- pyramidal neuron development Source: MGI
- regulation of cell migration Source: GO_Central
- regulation of cell shape Source: GO_Central
- regulation of GTPase activity Source: GO_Central
- semaphorin-plexin signaling pathway involved in axon guidance Source: ParkinsonsUK-UCL
- trigeminal nerve structural organization Source: ParkinsonsUK-UCL
Keywordsi
Molecular function | Receptor |
Enzyme and pathway databases
Reactomei | R-MMU-399954, Sema3A PAK dependent Axon repulsion R-MMU-399955, SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion R-MMU-399956, CRMPs in Sema3A signaling |
Names & Taxonomyi
Protein namesi | Recommended name: Plexin-A3 |
Gene namesi | Name:Plxna3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:107683, Plxna3 |
VEuPathDBi | HostDB:ENSMUSG00000031398 |
Subcellular locationi
Plasma membrane
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
Other locations
- semaphorin receptor complex Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 20 – 1220 | ExtracellularSequence analysisAdd BLAST | 1201 | |
Transmembranei | 1221 – 1241 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1242 – 1872 | CytoplasmicSequence analysisAdd BLAST | 631 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 1279 | L → R: Strongly reduced response to semaphorin. 1 Publication | 1 | |
Mutagenesisi | 1300 | Y → A: Almost abolishes response to semaphorin. 1 Publication | 1 | |
Mutagenesisi | 1309 | F → A: Almost abolishes response to semaphorin. 1 Publication | 1 | |
Mutagenesisi | 1407 – 1408 | RR → AA: Abolishes response to semaphorin. 1 Publication | 2 | |
Mutagenesisi | 1453 | I → R: Abolishes response to semaphorin. 1 Publication | 1 | |
Mutagenesisi | 1464 | L → G: Almost abolishes response to semaphorin. 1 Publication | 1 | |
Mutagenesisi | 1506 | Q → A: Abolishes response to semaphorin. 1 Publication | 1 | |
Mutagenesisi | 1631 – 1632 | HL → AA: Abolishes response to semaphorin. 1 Publication | 2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
ChainiPRO_0000411104 | 20 – 1872 | Plexin-A3Add BLAST | 1853 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 60 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 78 ↔ 87 | PROSITE-ProRule annotation | ||
Disulfide bondi | 113 ↔ 121 | PROSITE-ProRule annotation | ||
Disulfide bondi | 267 ↔ 388 | PROSITE-ProRule annotation | ||
Disulfide bondi | 283 ↔ 339 | PROSITE-ProRule annotation | ||
Disulfide bondi | 357 ↔ 376 | PROSITE-ProRule annotation | ||
Disulfide bondi | 492 ↔ 509 | PROSITE-ProRule annotation | ||
Disulfide bondi | 498 ↔ 540 | PROSITE-ProRule annotation | ||
Disulfide bondi | 501 ↔ 518 | PROSITE-ProRule annotation | ||
Disulfide bondi | 512 ↔ 524 | PROSITE-ProRule annotation | ||
Glycosylationi | 549 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 575 ↔ 595 | PROSITE-ProRule annotation | ||
Glycosylationi | 1163 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 1597 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
MaxQBi | P70208 |
PaxDbi | P70208 |
PeptideAtlasi | P70208 |
PRIDEi | P70208 |
ProteomicsDBi | 289630 [P70208-1] 289631 [P70208-2] |
PTM databases
GlyConnecti | 2591, 1 N-Linked glycan (1 site) |
GlyGeni | P70208, 4 sites, 1 N-linked glycan (1 site) |
iPTMneti | P70208 |
PhosphoSitePlusi | P70208 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000031398, Expressed in cerebral cortex and 287 other tissues |
ExpressionAtlasi | P70208, baseline and differential |
Genevisiblei | P70208, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 202263, 4 interactors |
DIPi | DIP-48959N |
STRINGi | 10090.ENSMUSP00000004326 |
Miscellaneous databases
RNActi | P70208, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P70208 |
SMRi | P70208 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P70208 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 20 – 489 | SemaPROSITE-ProRule annotationAdd BLAST | 470 | |
Domaini | 841 – 934 | IPT/TIG 1Add BLAST | 94 | |
Domaini | 936 – 1021 | IPT/TIG 2Add BLAST | 86 | |
Domaini | 1024 – 1123 | IPT/TIG 3Add BLAST | 100 | |
Domaini | 1126 – 1212 | IPT/TIG 4Add BLAST | 87 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 1240 – 1294 | Sequence analysisAdd BLAST | 55 |
Sequence similaritiesi
Keywords - Domaini
Coiled coil, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3610, Eukaryota |
GeneTreei | ENSGT01020000230473 |
HOGENOMi | CLU_001436_2_0_1 |
InParanoidi | P70208 |
OMAi | VYYNCSV |
PhylomeDBi | P70208 |
TreeFami | TF312962 |
Family and domain databases
Gene3Di | 1.10.506.10, 1 hit 2.130.10.10, 1 hit 2.60.40.10, 5 hits |
InterProi | View protein in InterPro IPR013783, Ig-like_fold IPR014756, Ig_E-set IPR002909, IPT_dom IPR031148, Plexin IPR013548, Plexin_cytoplasmic_RasGAP_dom IPR002165, Plexin_repeat IPR016201, PSI IPR008936, Rho_GTPase_activation_prot IPR001627, Semap_dom IPR036352, Semap_dom_sf IPR041019, TIG1_plexin IPR041362, TIG2_plexin IPR015943, WD40/YVTN_repeat-like_dom_sf |
PANTHERi | PTHR22625, PTHR22625, 1 hit |
Pfami | View protein in Pfam PF08337, Plexin_cytopl, 1 hit PF01437, PSI, 2 hits PF01403, Sema, 1 hit PF01833, TIG, 4 hits PF18020, TIG_2, 1 hit PF17960, TIG_plexin, 1 hit |
SMARTi | View protein in SMART SM00429, IPT, 4 hits SM00423, PSI, 3 hits SM00630, Sema, 1 hit |
SUPFAMi | SSF101912, SSF101912, 1 hit SSF48350, SSF48350, 1 hit SSF81296, SSF81296, 4 hits |
PROSITEi | View protein in PROSITE PS51004, SEMA, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPTVCLLPLL FFTIGGCLGS SRPFRTFVVT DTTLTHLAVH RVTGEVFVGA
60 70 80 90 100
VNRVFKLAPN LTELRAHVTG PIEDNARCYP PPSMRVCSHR LVPVDNVNKL
110 120 130 140 150
LLIDYAARRL VACGSIWQGI CQFLRLDDLF KLGEPHHRKE HYLSGAQEPD
160 170 180 190 200
SMAGVIVEQV QGPSKLFVGT AVDGKSEYFP TLSSRKLIDD EDSGDMFSLV
210 220 230 240 250
YQDEFVSSQI KIPSDTLSLY PAFDIYYIYG FVSASFVYFL TLQLDTQQTL
260 270 280 290 300
LDTAGEKFFT SKIVRMCAGD SEFYSYVEFP IGCSWRGVEY RLVQSAHLAK
310 320 330 340 350
PGLLLAQALG VPADEDVLFT IFSQGQKNRA NPPRQTILCL FTLSSINAHI
360 370 380 390 400
RRRIQSCYRG EGTLALPWLL NKELPCINTP LQINGNFCGL VLNQPLGGLH
410 420 430 440 450
VIEGLPLLAD STDGMASVAA YTYHQHSVVF IGTRSGNLKK VRVDGSQDAQ
460 470 480 490 500
LYETVSVVQG SPILRDLLFS PDHRHIYLLS EKQVSQLPVE TCEQYLSCAA
510 520 530 540 550
CLGSGDPHCG WCVLQHRCCR EGACPGASAP HGFAEELSKC IQVRVRPNNV
560 570 580 590 600
SVTSSGVQLT VAMRNVPDLS VGVSCSFEEV TESEAILLPS GELRCPSPSL
610 620 630 640 650
QELQTLTRGH GATHTVRLQL LSMETGVRFA GVDFVFYNCS ALQSCMSCVG
660 670 680 690 700
SPYPCHWCKY RHVCTSHPHE CSFQEGRVHS PEGCPEILPQ GDLLIPVGVM
710 720 730 740 750
QPLTLRAKNL PQPQSGQKNY ECVVRVQGRQ HRVPAVRFNS SSVQCQNASY
760 770 780 790 800
FYEGDEFGDT ELDFSVVWDG DFPIDKPPSF RALLYKCWAQ RPSCGLCLKA
810 820 830 840 850
DPRFNCGWCI SEHRCQLRAH CPAPKSNWMH PSQKGARCSH PRITQIHPLT
860 870 880 890 900
GPKEGGTRVT IVGENLGLTS REVGLRVAGV RCNSIPTEYV SAERIVCEME
910 920 930 940 950
ESLVPSPPPG PAELCVGDCS ADFRTQSQQL YSFVTPTFDR VSPSRGPASG
960 970 980 990 1000
GTRLTISGIS LDAGSRVTVI IRDGECQFVR RDAEAIVCIS PVSTLGPSQS
1010 1020 1030 1040 1050
PITLAIDHAN ISNTGVIYTY TQDPTVTHLE PTWSIINGST SITVSGTHLL
1060 1070 1080 1090 1100
TVQEPRVRAK YRGIETTNTC QVINDTAMLC KAPGIFLGHP QPRAQGEHPD
1110 1120 1130 1140 1150
EFGFLLDHVQ AARSLNRSSF TYYPDPSFEP LGPSGVLDVK PGSHVVLKGK
1160 1170 1180 1190 1200
NLIPAAAGSS RLNYTVLIGG QPCALTVSDT QLLCDSPSQT GRQPVMVLVG
1210 1220 1230 1240 1250
GLEFWLGTLH ITADRALTLP AMVGLAAGGG LLLLAITVVL VAYKRKTQDA
1260 1270 1280 1290 1300
DRTLKRLQLQ MDNLESRVAL ECKEAFAELQ TDINELTNHM DGVQIPFLDY
1310 1320 1330 1340 1350
RTYAVRVLFP GIEAHPVLKE LDTPPNVEKA LRLFGQLLHS RAFLLTFIHT
1360 1370 1380 1390 1400
LEAQSSFSMR DRGTVASLTM VALQSRLDYA TGLLKQLLAD LIEKNLESKN
1410 1420 1430 1440 1450
HPKLLLRRTE SVAEKMLTNW FTFLLHKFLK ECAGEPLFLL YCAIKQQMEK
1460 1470 1480 1490 1500
GPIDAITGEA RYSLSEDKLI RQQIDYKTLT LHCVCPESEG SAQVPVKVLN
1510 1520 1530 1540 1550
CDSITQAKDK LLDTVYKGIP YSQRPKAEDM DLEWRQGRMA RIILQDEDIT
1560 1570 1580 1590 1600
TKIECDWKRV NSLAHYQVTD GSLVALVPKQ VSAYNMANSF TFTRSLSRYE
1610 1620 1630 1640 1650
SLLRAASSPD SLRSRAPMLT PDQEAGTKLW HLVRNHDHTD HREGDRGSKM
1660 1670 1680 1690 1700
VSEIYLTRLL ATKGTLQKFV DDLFETVFST AHRGSALPLA IKYMFDFLDE
1710 1720 1730 1740 1750
QADQRQISDP DVRHTWKSNC LPLRFWVNVI KNPQFVFDIH KNSITDACLS
1760 1770 1780 1790 1800
VVAQTFMDSC STSEHRLGKD SPSNKLLYAK DIPNYKSWVE RYYRDIAKMA
1810 1820 1830 1840 1850
SISDQDMDAY LVEQSRLHAN DFNVLSALSE LYFYVTKYRQ EILTSLDRDA
1860 1870
SCRKHKLRQK LEQIITLVSS SS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1627 | T → A in BAC33681 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 1861 | L → P in BAA13190 (PubMed:8806646).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_041609 | 1072 – 1148 | Missing in isoform 2. 1 PublicationAdd BLAST | 77 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D86950 mRNA Translation: BAA13190.1 BC093482 mRNA Translation: AAH93482.1 AJ748653 mRNA Translation: CAG38687.1 AK049319 mRNA Translation: BAC33681.1 |
CCDSi | CCDS30228.1 [P70208-1] |
PIRi | JC4976 |
RefSeqi | NP_032909.2, NM_008883.2 [P70208-1] XP_006527963.1, XM_006527900.2 |
Genome annotation databases
Ensembli | ENSMUST00000004326; ENSMUSP00000004326; ENSMUSG00000031398 [P70208-1] |
GeneIDi | 18846 |
KEGGi | mmu:18846 |
UCSCi | uc009too.1, mouse [P70208-1] uc012hku.1, mouse [P70208-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D86950 mRNA Translation: BAA13190.1 BC093482 mRNA Translation: AAH93482.1 AJ748653 mRNA Translation: CAG38687.1 AK049319 mRNA Translation: BAC33681.1 |
CCDSi | CCDS30228.1 [P70208-1] |
PIRi | JC4976 |
RefSeqi | NP_032909.2, NM_008883.2 [P70208-1] XP_006527963.1, XM_006527900.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3IG3 | X-ray | 1.99 | A | 1247-1872 | [»] | |
AlphaFoldDBi | P70208 | |||||
SMRi | P70208 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 202263, 4 interactors |
DIPi | DIP-48959N |
STRINGi | 10090.ENSMUSP00000004326 |
PTM databases
GlyConnecti | 2591, 1 N-Linked glycan (1 site) |
GlyGeni | P70208, 4 sites, 1 N-linked glycan (1 site) |
iPTMneti | P70208 |
PhosphoSitePlusi | P70208 |
Proteomic databases
MaxQBi | P70208 |
PaxDbi | P70208 |
PeptideAtlasi | P70208 |
PRIDEi | P70208 |
ProteomicsDBi | 289630 [P70208-1] 289631 [P70208-2] |
Protocols and materials databases
Antibodypediai | 31267, 57 antibodies from 18 providers |
DNASUi | 18846 |
Genome annotation databases
Ensembli | ENSMUST00000004326; ENSMUSP00000004326; ENSMUSG00000031398 [P70208-1] |
GeneIDi | 18846 |
KEGGi | mmu:18846 |
UCSCi | uc009too.1, mouse [P70208-1] uc012hku.1, mouse [P70208-2] |
Organism-specific databases
CTDi | 55558 |
MGIi | MGI:107683, Plxna3 |
VEuPathDBi | HostDB:ENSMUSG00000031398 |
Phylogenomic databases
eggNOGi | KOG3610, Eukaryota |
GeneTreei | ENSGT01020000230473 |
HOGENOMi | CLU_001436_2_0_1 |
InParanoidi | P70208 |
OMAi | VYYNCSV |
PhylomeDBi | P70208 |
TreeFami | TF312962 |
Enzyme and pathway databases
Reactomei | R-MMU-399954, Sema3A PAK dependent Axon repulsion R-MMU-399955, SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion R-MMU-399956, CRMPs in Sema3A signaling |
Miscellaneous databases
BioGRID-ORCSi | 18846, 1 hit in 72 CRISPR screens |
ChiTaRSi | Plxna3, mouse |
EvolutionaryTracei | P70208 |
PROi | PR:P70208 |
RNActi | P70208, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031398, Expressed in cerebral cortex and 287 other tissues |
ExpressionAtlasi | P70208, baseline and differential |
Genevisiblei | P70208, MM |
Family and domain databases
Gene3Di | 1.10.506.10, 1 hit 2.130.10.10, 1 hit 2.60.40.10, 5 hits |
InterProi | View protein in InterPro IPR013783, Ig-like_fold IPR014756, Ig_E-set IPR002909, IPT_dom IPR031148, Plexin IPR013548, Plexin_cytoplasmic_RasGAP_dom IPR002165, Plexin_repeat IPR016201, PSI IPR008936, Rho_GTPase_activation_prot IPR001627, Semap_dom IPR036352, Semap_dom_sf IPR041019, TIG1_plexin IPR041362, TIG2_plexin IPR015943, WD40/YVTN_repeat-like_dom_sf |
PANTHERi | PTHR22625, PTHR22625, 1 hit |
Pfami | View protein in Pfam PF08337, Plexin_cytopl, 1 hit PF01437, PSI, 2 hits PF01403, Sema, 1 hit PF01833, TIG, 4 hits PF18020, TIG_2, 1 hit PF17960, TIG_plexin, 1 hit |
SMARTi | View protein in SMART SM00429, IPT, 4 hits SM00423, PSI, 3 hits SM00630, Sema, 1 hit |
SUPFAMi | SSF101912, SSF101912, 1 hit SSF48350, SSF48350, 1 hit SSF81296, SSF81296, 4 hits |
PROSITEi | View protein in PROSITE PS51004, SEMA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PLXA3_MOUSE | |
Accessioni | P70208Primary (citable) accession number: P70208 Secondary accession number(s): A5D6Q5, Q684J0, Q8BWZ5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 27, 2011 |
Last sequence update: | July 27, 2011 | |
Last modified: | May 25, 2022 | |
This is version 150 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families