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Protein

Proteasome subunit beta type-7

Gene

Psmb7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Within the 20S core complex, PSMB7 displays a trypsin-like activity.2 Publications

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei44NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-MMU-1169091 Activation of NF-kappaB in B cells
R-MMU-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-MMU-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-MMU-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-MMU-174113 SCF-beta-TrCP mediated degradation of Emi1
R-MMU-174154 APC/C:Cdc20 mediated degradation of Securin
R-MMU-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-MMU-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-MMU-187577 SCF(Skp2)-mediated degradation of p27/p21
R-MMU-195253 Degradation of beta-catenin by the destruction complex
R-MMU-202424 Downstream TCR signaling
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2871837 FCERI mediated NF-kB activation
R-MMU-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-MMU-350562 Regulation of ornithine decarboxylase (ODC)
R-MMU-382556 ABC-family proteins mediated transport
R-MMU-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-MMU-4608870 Asymmetric localization of PCP proteins
R-MMU-4641257 Degradation of AXIN
R-MMU-4641258 Degradation of DVL
R-MMU-5358346 Hedgehog ligand biogenesis
R-MMU-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-MMU-5607764 CLEC7A (Dectin-1) signaling
R-MMU-5610780 Degradation of GLI1 by the proteasome
R-MMU-5610785 GLI3 is processed to GLI3R by the proteasome
R-MMU-5632684 Hedgehog 'on' state
R-MMU-5658442 Regulation of RAS by GAPs
R-MMU-5668541 TNFR2 non-canonical NF-kB pathway
R-MMU-5676590 NIK-->noncanonical NF-kB signaling
R-MMU-5687128 MAPK6/MAPK4 signaling
R-MMU-5689603 UCH proteinases
R-MMU-5689880 Ub-specific processing proteases
R-MMU-6798695 Neutrophil degranulation
R-MMU-68827 CDT1 association with the CDC6:ORC:origin complex
R-MMU-68949 Orc1 removal from chromatin
R-MMU-69017 CDK-mediated phosphorylation and removal of Cdc6
R-MMU-69229 Ubiquitin-dependent degradation of Cyclin D1
R-MMU-69481 G2/M Checkpoints
R-MMU-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-MMU-8852276 The role of GTSE1 in G2/M progression after G2 checkpoint
R-MMU-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-MMU-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-MMU-8939902 Regulation of RUNX2 expression and activity
R-MMU-8941858 Regulation of RUNX3 expression and activity
R-MMU-8948751 Regulation of PTEN stability and activity
R-MMU-9020702 Interleukin-1 signaling
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Protein family/group databases

MEROPSiT01.011

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-7 (EC:3.4.25.1)
Alternative name(s):
Macropain chain Z
Multicatalytic endopeptidase complex chain Z
Proteasome subunit Z
Gene namesi
Name:Psmb7
Synonyms:Mmc14
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:107637 Psmb7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000266471 – 43Removed in mature formBy similarityAdd BLAST43
ChainiPRO_000002664844 – 277Proteasome subunit beta type-7Add BLAST234

Keywords - PTMi

Zymogen

Proteomic databases

EPDiP70195
MaxQBiP70195
PaxDbiP70195
PeptideAtlasiP70195
PRIDEiP70195

2D gel databases

REPRODUCTION-2DPAGEiIPI00136483
P70195

PTM databases

iPTMnetiP70195
PhosphoSitePlusiP70195
SwissPalmiP70195

Expressioni

Inductioni

Up-regulated by the antioxidant dithiolethione (D3T) in colon (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000026750
GenevisibleiP70195 MM

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7.By similarity2 Publications

Protein-protein interaction databases

BioGridi202424, 1 interactor
CORUMiP70195
IntActiP70195, 5 interactors
MINTiP70195
STRINGi10090.ENSMUSP00000028083

Structurei

Secondary structure

1277
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi46 – 50Combined sources5
Beta strandi52 – 60Combined sources9
Beta strandi63 – 65Combined sources3
Beta strandi68 – 73Combined sources6
Beta strandi77 – 91Combined sources15
Helixi92 – 113Combined sources22
Helixi119 – 132Combined sources14
Turni133 – 135Combined sources3
Beta strandi139 – 146Combined sources8
Beta strandi151 – 156Combined sources6
Beta strandi162 – 164Combined sources3
Beta strandi166 – 171Combined sources6
Helixi174 – 184Combined sources11
Helixi191 – 208Combined sources18
Beta strandi216 – 224Combined sources9
Beta strandi226 – 233Combined sources8
Beta strandi254 – 261Combined sources8

3D structure databases

ProteinModelPortaliP70195
SMRiP70195
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0173 Eukaryota
COG0638 LUCA
GeneTreeiENSGT00510000046533
HOGENOMiHOG000182856
HOVERGENiHBG093416
InParanoidiP70195
KOiK02739
OMAiDLCVIRK
OrthoDBiEOG091G0E5S
PhylomeDBiP70195
TreeFamiTF106222

Family and domain databases

Gene3Di3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR029055 Ntn_hydrolases_N
IPR000243 Pept_T1A_subB
IPR035216 Proteasome_beta7
IPR024689 Proteasome_bsu_C
IPR016050 Proteasome_bsu_CS
IPR001353 Proteasome_sua/b
IPR023333 Proteasome_suB-type
PANTHERiPTHR11599:SF42 PTHR11599:SF42, 1 hit
PfamiView protein in Pfam
PF12465 Pr_beta_C, 1 hit
PF00227 Proteasome, 1 hit
PRINTSiPR00141 PROTEASOME
SUPFAMiSSF56235 SSF56235, 1 hit
PROSITEiView protein in PROSITE
PS00854 PROTEASOME_BETA_1, 1 hit
PS51476 PROTEASOME_BETA_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P70195-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVSVFQPP VGGFSFDNCR RNAVLEADFA KKGFKLPKAR KTGTTIAGVV
60 70 80 90 100
YKDGIVLGAD TRATEGMVVA DKNCSKIHFI SPNIYCCGAG TAADTDMTTQ
110 120 130 140 150
LISSNLELHS LTTGRLPRVV TANRMLKQML FRYQGYIGAA LVLGGVDVTG
160 170 180 190 200
PHLYSIYPHG STDKLPYVTM GSGSLAAMAV FEDKFRPDME EEEAKKLVSE
210 220 230 240 250
AIAAGIFNDL GSGSNIDLCV ISKSKLDFLR PFSVPNKKGT RLGRYRCEKG
260 270
TTAVLTEKVT PLEIEVLEET VQTMDTS
Length:277
Mass (Da):29,891
Last modified:February 1, 1997 - v1
Checksum:i3B8BA01B1E6392F2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti108L → F in BAB28354 (PubMed:16141072).Curated1
Sequence conflicti237K → E in BAB22385 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83585 mRNA Translation: BAA12017.1
Y10874 mRNA Translation: CAA71824.1
D85570 Genomic DNA Translation: BAA22857.1
AK002823 mRNA Translation: BAB22385.1
AK012613 mRNA Translation: BAB28354.1
AK013961 mRNA Translation: BAB29085.1
AK075759 mRNA Translation: BAC35937.1
AK088276 mRNA Translation: BAC40251.1
AK088765 mRNA Translation: BAC40556.1
AK168823 mRNA Translation: BAE40650.1
BC057662 mRNA Translation: AAH57662.1
CCDSiCCDS16010.1
PIRiJC6122
RefSeqiNP_035317.1, NM_011187.1
UniGeneiMm.389251

Genome annotation databases

EnsembliENSMUST00000028083; ENSMUSP00000028083; ENSMUSG00000026750
GeneIDi19177
KEGGimmu:19177
UCSCiuc008jnp.1 mouse

Similar proteinsi

Entry informationi

Entry nameiPSB7_MOUSE
AccessioniPrimary (citable) accession number: P70195
Secondary accession number(s): O09084
, Q542F7, Q9CZH4, Q9DCF7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: February 1, 1997
Last modified: June 20, 2018
This is version 168 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

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