Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Double C2-like domain-containing protein beta

Gene

Doc2b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calcium sensor which positively regulates SNARE-dependent fusion of vesicles with membranes. Binds phospholipids in a calcium-dependent manner and may act at the priming stage of fusion by modifying membrane curvature to stimulate fusion. Involved in calcium-triggered exocytosis in chromaffin cells and calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Involved both in glucose-stimulated insulin secretion in pancreatic cells and insulin-dependent GLUT4 transport to the plasma membrane in adipocytes.4 Publications

GO - Molecular functioni

  • calcium-dependent phospholipid binding Source: UniProtKB
  • calcium ion binding Source: GO_Central
  • clathrin binding Source: GO_Central
  • syntaxin binding Source: UniProtKB

GO - Biological processi

  • calcium ion-regulated exocytosis of neurotransmitter Source: UniProtKB
  • positive regulation of calcium ion-dependent exocytosis Source: UniProtKB
  • positive regulation of insulin secretion Source: UniProtKB
  • positive regulation of vesicle fusion Source: UniProtKB
  • protein localization Source: UniProtKB
  • regulation of calcium ion-dependent exocytosis Source: GO_Central
  • vesicle fusion Source: GO_Central

Keywordsi

LigandCalcium, Calcium/phospholipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Double C2-like domain-containing protein beta
Short name:
Doc2-beta
Gene namesi
Name:Doc2b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1100497 Doc2b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are viable and fertile without gross abnormalities.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi157D → N: Loss of interaction with STX4 and insulin-dependent translocation to the cell membrane; when associated with N-163, N-297 and N-303. 1 Publication1
Mutagenesisi163D → N: Loss of interaction with STX4 and insulin-dependent translocation to the cell membrane; when associated with N-157, N-297 and N-303. 1 Publication1
Mutagenesisi297D → N: Loss of interaction with STX4 and insulin-dependent translocation to the cell membrane; when associated with N-157, N-163 and N-303. 1 Publication1
Mutagenesisi303D → N: Loss of interaction with STX4 and insulin-dependent translocation to the cell membrane; when associated with N-157, N-163 and N-297. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000799691 – 412Double C2-like domain-containing protein betaAdd BLAST412

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei411PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP70169
PaxDbiP70169
PeptideAtlasiP70169
PRIDEiP70169

PTM databases

iPTMnetiP70169
PhosphoSitePlusiP70169

Expressioni

Tissue specificityi

Widely expressed. Expressed in pancreatic islet cells (at protein level).2 Publications

Gene expression databases

BgeeiENSMUSG00000020848
CleanExiMM_DOC2B
GenevisibleiP70169 MM

Interactioni

Subunit structurei

Interacts with cytoplasmic dynein light chain DYNLT1. May interact with UNC13A; the interaction mediates targeting to the plasma membrane (By similarity). Probably interacts with the SNARE (soluble N-ethylmaleimide-sensitive factor attached protein receptor) complex composed of SNAP25, STX1A and VAMP2; the interaction is calcium-dependent and competitive with SYT1. Interacts with STX4; the interaction is calcium-dependent, increased by insulin and glucose, and mediates vesicle fusion with plasma membrane in pancreatic cells and adipocytes. Interacts with STXBP3; the interaction is direct, occurs at the cell membrane and regulates glucose-stimulated insulin secretion.By similarity4 Publications

GO - Molecular functioni

  • clathrin binding Source: GO_Central
  • syntaxin binding Source: UniProtKB

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021209

Structurei

3D structure databases

ProteinModelPortaliP70169
SMRiP70169
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini128 – 232C2 1PROSITE-ProRule annotationAdd BLAST105
Domaini268 – 371C2 2PROSITE-ProRule annotationAdd BLAST104

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 90Mediates interaction with DYNLT1By similarityAdd BLAST90
Regioni1 – 36Negatively regulates targeting to plasma membraneAdd BLAST36
Regioni257 – 375Mediates interaction with STXBP31 PublicationAdd BLAST119

Domaini

C2 domain 1 is involved in binding calcium and phospholipids. According to PubMed:19033398, the C2 domain 2 may also play a role in the calcium-dependent targeting to membranes.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1013 Eukaryota
ENOG410XQXA LUCA
GeneTreeiENSGT00810000125376
HOGENOMiHOG000232127
HOVERGENiHBG051388
InParanoidiP70169
KOiK19917
OMAiKANHTKT
OrthoDBiEOG091G0FPP
TreeFamiTF351844

Family and domain databases

Gene3Di2.60.40.150, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR014638 Doc2
IPR030534 Doc2b
IPR001565 Synaptotagmin
PANTHERiPTHR10024:SF204 PTHR10024:SF204, 2 hits
PfamiView protein in Pfam
PF00168 C2, 2 hits
PIRSFiPIRSF036931 Doc2, 1 hit
PRINTSiPR00360 C2DOMAIN
PR00399 SYNAPTOTAGMN
SMARTiView protein in SMART
SM00239 C2, 2 hits
PROSITEiView protein in PROSITE
PS50004 C2, 2 hits

Sequencei

Sequence statusi: Complete.

P70169-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLRRRGEKA TISIQEHMAI DVCPGPIRPI KQISDYFPRF PRGLPPTAAP
60 70 80 90 100
RAPAPPDAPA RSPAASASPR SPSDGARDDD EDVDQLFGAY GASPGPSPGP
110 120 130 140 150
SPARPPAKPP EDEPDVDGYE SDDCTALGTL DFSLLYDQEN NALHCTISKA
160 170 180 190 200
KGLKPMDHNG LADPYVKLHL LPGASKANKL RTKTLRNTLN PSWNETLTYY
210 220 230 240 250
GITDEDMVRK TLRISVCDED KFRHNEFIGE TRVPLKKLKP NHTKTFSICL
260 270 280 290 300
EKQLPVDKAE DKSLEERGRI LISLKYSSQK QGLLVGIVRC AHLAAMDANG
310 320 330 340 350
YSDPYVKTYL KPDVDKKSKH KTAVKKKTLN PEFNEEFCYE IKHGDLAKKT
360 370 380 390 400
LEVTVWDYDI GKSNDFIGGV VLGINAKGER LKHWFDCLKN KDKRIERWHT
410
LTNELPGAVL SD
Length:412
Mass (Da):45,853
Last modified:July 27, 2011 - v2
Checksum:i4E94480BEDE91722
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti389K → N in BAA12714 (PubMed:8902635).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85037 mRNA Translation: BAA12714.1
AL669897 Genomic DNA Translation: CAI26032.1
CH466596 Genomic DNA Translation: EDL12842.1
BC067030 mRNA Translation: AAH67030.1
CCDSiCCDS25057.1
PIRiJC4921
RefSeqiNP_031899.2, NM_007873.3
UniGeneiMm.5137

Genome annotation databases

EnsembliENSMUST00000021209; ENSMUSP00000021209; ENSMUSG00000020848
GeneIDi13447
KEGGimmu:13447
UCSCiuc007kev.1 mouse

Similar proteinsi

Entry informationi

Entry nameiDOC2B_MOUSE
AccessioniPrimary (citable) accession number: P70169
Secondary accession number(s): Q5SS41, Q6NXK3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: July 27, 2011
Last modified: March 28, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health