UniProtKB - P69937 (GDX_ECOLI)
Protein
Guanidinium exporter
Gene
gdx
Organism
Escherichia coli (strain K12)
Status
Functioni
Guanidinium ion exporter. Couples guanidinium export to the proton motive force, exchanging one guanidinium ion for two protons (PubMed:29507227). Overexpression leads to resistance to a subset of toxic quaternary ammonium compounds such as cetylpyridinium, cetyldimethylethyl ammonium and cetrimide cations (PubMed:11948170).2 Publications
Caution
Was originally (PubMed:8096175) thought to suppress a groEL mutation and mimic the effect of groE overexpression. This was later shown (PubMed:11948170) to be probably due to an artifact.2 Publications
GO - Molecular functioni
- solute:proton antiporter activity Source: EcoCyc
- transmembrane transporter activity Source: GO_Central
GO - Biological processi
- transmembrane transport Source: GO_Central
- xenobiotic detoxification by transmembrane export across the plasma membrane Source: EcoCyc
Keywordsi
Biological process | Ion transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:SUGE-MONOMER MetaCyc:SUGE-MONOMER |
Protein family/group databases
TCDBi | 2.A.7.1.4, the drug/metabolite transporter (dmt) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Guanidinium exporter1 PublicationAlternative name(s): Quaternary ammonium compound-resistance protein SugECurated |
Gene namesi | Name:gdx1 Publication Synonyms:sugE1 Publication Ordered Locus Names:b4148, JW5738 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 2 Publications; Multi-pass membrane protein 1 Publication
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 1 – 21 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 22 – 28 | Cytoplasmic1 Publication | 7 | |
Transmembranei | 29 – 49 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 50 – 57 | Periplasmic1 Publication | 8 | |
Transmembranei | 58 – 78 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 79 – 81 | Cytoplasmic1 Publication | 3 | |
Transmembranei | 82 – 102 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 103 – 105 | Periplasmic1 Publication | 3 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000108095 | 1 – 105 | Guanidinium exporterAdd BLAST | 105 |
Proteomic databases
PaxDbi | P69937 |
Expressioni
Inductioni
Transcriptionally regulated by guanidine, via a guanidine-sensing riboswitch.1 Publication
Interactioni
Subunit structurei
Homodimer. The subunits assemble with antiparallel topology.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4262698, 178 interactors |
STRINGi | 511145.b4148 |
Family & Domainsi
Sequence similaritiesi
Belongs to the drug/metabolite transporter (DMT) superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family. Gdx/SugE subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2076, Bacteria |
HOGENOMi | CLU_133067_1_2_6 |
InParanoidi | P69937 |
PhylomeDBi | P69937 |
Family and domain databases
InterProi | View protein in InterPro IPR000390, Small_multidrug_res |
PANTHERi | PTHR30561, PTHR30561, 1 hit |
Pfami | View protein in Pfam PF00893, Multi_Drug_Res, 1 hit |
i Sequence
Sequence statusi: Complete.
P69937-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSWIILVIAG LLEVVWAVGL KYTHGFSRLT PSVITVTAMI VSMALLAWAM
60 70 80 90 100
KSLPVGTAYA VWTGIGAVGA AITGIVLLGE SANPMRLASL ALIVLGIIGL
KLSTH
Sequence cautioni
The sequence AAA97047 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence CAA49570 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X69949 Genomic DNA Translation: CAA49570.1 Different initiation. X69949 Genomic DNA Translation: CAA49571.1 U21726 Genomic DNA Translation: AAC46453.1 U14003 Genomic DNA Translation: AAA97047.1 Different initiation. U00096 Genomic DNA Translation: AAC77108.2 AP009048 Genomic DNA Translation: BAE78152.1 |
PIRi | S56376 |
RefSeqi | NP_418572.4, NC_000913.3 WP_000118482.1, NZ_STEB01000014.1 |
Genome annotation databases
EnsemblBacteriai | AAC77108; AAC77108; b4148 BAE78152; BAE78152; BAE78152 |
GeneIDi | 58391490 948671 |
KEGGi | ecj:JW5738 eco:b4148 |
PATRICi | fig|1411691.4.peg.2550 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X69949 Genomic DNA Translation: CAA49570.1 Different initiation. X69949 Genomic DNA Translation: CAA49571.1 U21726 Genomic DNA Translation: AAC46453.1 U14003 Genomic DNA Translation: AAA97047.1 Different initiation. U00096 Genomic DNA Translation: AAC77108.2 AP009048 Genomic DNA Translation: BAE78152.1 |
PIRi | S56376 |
RefSeqi | NP_418572.4, NC_000913.3 WP_000118482.1, NZ_STEB01000014.1 |
3D structure databases
SMRi | P69937 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262698, 178 interactors |
STRINGi | 511145.b4148 |
Protein family/group databases
TCDBi | 2.A.7.1.4, the drug/metabolite transporter (dmt) superfamily |
Proteomic databases
PaxDbi | P69937 |
Genome annotation databases
EnsemblBacteriai | AAC77108; AAC77108; b4148 BAE78152; BAE78152; BAE78152 |
GeneIDi | 58391490 948671 |
KEGGi | ecj:JW5738 eco:b4148 |
PATRICi | fig|1411691.4.peg.2550 |
Organism-specific databases
EchoBASEi | EB1573 |
Phylogenomic databases
eggNOGi | COG2076, Bacteria |
HOGENOMi | CLU_133067_1_2_6 |
InParanoidi | P69937 |
PhylomeDBi | P69937 |
Enzyme and pathway databases
BioCyci | EcoCyc:SUGE-MONOMER MetaCyc:SUGE-MONOMER |
Miscellaneous databases
PROi | PR:P69937 |
Family and domain databases
InterProi | View protein in InterPro IPR000390, Small_multidrug_res |
PANTHERi | PTHR30561, PTHR30561, 1 hit |
Pfami | View protein in Pfam PF00893, Multi_Drug_Res, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GDX_ECOLI | |
Accessioni | P69937Primary (citable) accession number: P69937 Secondary accession number(s): P30743, Q2M6F4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 4, 2005 |
Last sequence update: | January 4, 2005 | |
Last modified: | April 7, 2021 | |
This is version 116 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families