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Entry version 118 (13 Nov 2019)
Sequence version 2 (24 Nov 2009)
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Protein

DnaA regulatory inactivator Hda

Gene

hda

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Mediates the interactions of DNA replication initiator protein DnaA with DNA polymerase subunit beta sliding clamp (dnaN). Stimulates hydrolysis of ATP-DnaA to ADP-DnaA, rendering DnaA inactive for reinitiation, a process called regulatory inhibition of DnaA or RIDA. ADP-binding activates Hda to hydrolyze DnaA-ATP; Hda monomers bind to ADP with about 200-fold greater affinity than for ATP. RIDA function can be genetically separated from viability, suggesting this protein has another function as well.
Suppresses the toxic effect of overexpressing a TrfA N-terminal 163 residue fragment. Inhibits inner membrane-associated plasmid IncP-alpha RK2 replication probably by interacting with plasmid-encoded TrfA.

Miscellaneous

Starts with a CTG codon.
There are about 50 homodimers per cell in strains C600 and K12 / MG1655.

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA replication inhibitor
Biological processDNA replication
LigandNucleotide-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:G7313-MONOMER
ECOL316407:JW5397-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
DnaA regulatory inactivator Hda
Alternative name(s):
DnaA paralog
Short name:
Dp
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:hda
Synonyms:idaB, yfgE
Ordered Locus Names:b2496, JW5397
ORF Names:f248c
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Essential in strain C600. Disruption in MG1655 confers cold-sensitivity with asynchronously replicating DNA, which is quickly suppressed. Increased levels of plasmid IncP-alpha RK2 in strain BL21-DE3, increased plasmid replication in vitro.3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi6Q → A: Severely impaired in beta clamp binding and DnaA-ATP hydrolysis, very poor growth at 30 degrees Celsius. 2 Publications1
Mutagenesisi7 – 11LSLPL → ASAPA: Decreased binding to beta clamp. 1 Publication5
Mutagenesisi9 – 10LP → AA: Decreased binding to beta clamp. 1 Publication2
Mutagenesisi9L → A: Impaired in beta clamp binding and DnaA-ATP hydrolysis, very poor growth at 30 degrees Celsius. 2 Publications1
Mutagenesisi56R → A: Severely impaired in ADP-binding but still has beta clamp-binding activity in vitro, defective in DnaA-ATP hydrolysis in vivo; when associated with 102-A-A-103. 1 Publication1
Mutagenesisi98L → P: No growth at 30 degrees Celsius, impaired RIDA. 1 Publication1
Mutagenesisi102 – 103DN → AA: Impaired in ADP-binding, has beta clamp-binding activity in vitro. 1 Publication2
Mutagenesisi136G → D: No growth at 30 degrees Celsius, impaired RIDA. 1 Publication1
Mutagenesisi153R → A or M: Defective in DnaA-ATP hydrolysis, binds beta clamp normally, no growth at 30 degrees Celsius. 2 Publications1
Mutagenesisi157G → V: Grows at 30 degrees Celsius, impaired RIDA. 1 Publication1
Mutagenesisi167D → N: Grows at 30 degrees Celsius, impaired RIDA. 1 Publication1
Mutagenesisi196R → Q: Grows at 30 degrees Celsius, impaired RIDA. 1 Publication1
Mutagenesisi208 – 210Missing : No growth at 30 degrees Celsius, impaired RIDA. 1 Publication3
Mutagenesisi210Q → QLDQ: No growth at 30 degrees Celsius, impaired RIDA. 1 Publication1
Mutagenesisi212D → N: Grows at 30 degrees Celsius, impaired RIDA. 1 Publication1
Mutagenesisi233L → F: No growth at 30 degrees Celsius, impaired RIDA. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001143141 – 233DnaA regulatory inactivator HdaAdd BLAST233

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P69931

PRoteomics IDEntifications database

More...
PRIDEi
P69931

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

The active form seems to be an ADP-bound monomer; apo-Hda forms homo-multimers that do not hydrolzye DnaA-bound ATP. Forms the RIDA complex (regulatory inactivation of DnaA) of ATP-DnaA, ADP-Hda and the DNA-loaded beta sliding clamp (dnaN).

Interacts with plasmid IncP-alpha RK2-encoded protein TrfA in strain B / BL21-DE3.

4 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
4261436, 151 interactors
851316, 5 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...
ComplexPortali
CPX-1945 DnaA-Hda complex
CPX-1954 Hda-beta clamp complex

Database of interacting proteins

More...
DIPi
DIP-48006N

Protein interaction database and analysis system

More...
IntActi
P69931, 14 interactors

STRING: functional protein association networks

More...
STRINGi
511145.b2496

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P69931

Database of comparative protein structure models

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ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni6 – 11Beta clamp binding motif6

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the DnaA family. HdA subfamily.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4108KZ1 Bacteria
COG0593 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000256538

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P69931

KEGG Orthology (KO)

More...
KOi
K10763

Database for complete collections of gene phylogenies

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PhylomeDBi
P69931

Family and domain databases

HAMAP database of protein families

More...
HAMAPi
MF_01158 Hda, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR020591 Chromosome_initiator_DnaA-like
IPR013317 DnaA
IPR017788 Hda
IPR022864 Hda_Enterobact
IPR027417 P-loop_NTPase

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00308 Bac_DnaA, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR00051 DNAA

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF52540 SSF52540, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR03420 DnaA_homol_Hda, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P69931-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNTPAQLSLP LYLPDDETFA SFWPGDNSSL LAALQNVLRQ EHSGYIYLWA
60 70 80 90 100
REGAGRSHLL HAACAELSQR GDAVGYVPLD KRTWFVPEVL DGMEHLSLVC
110 120 130 140 150
IDNIECIAGD ELWEMAIFDL YNRILESGKT RLLITGDRPP RQLNLGLPDL
160 170 180 190 200
ASRLDWGQIY KLQPLSDEDK LQALQLRARL RGFELPEDVG RFLLKRLDRE
210 220 230
MRTLFMTLDQ LDRASITAQR KLTIPFVKEI LKL
Length:233
Mass (Da):26,633
Last modified:November 24, 2009 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iD25C7CDF31DAF7DC
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75549.2
AP009048 Genomic DNA Translation: BAA16384.1

Protein sequence database of the Protein Information Resource

More...
PIRi
G65025

NCBI Reference Sequences

More...
RefSeqi
NP_416991.2, NC_000913.3

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC75549; AAC75549; b2496
BAA16384; BAA16384; BAA16384

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
946977

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW5397
eco:b2496

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|511145.12.peg.2592

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75549.2
AP009048 Genomic DNA Translation: BAA16384.1
PIRiG65025
RefSeqiNP_416991.2, NC_000913.3

3D structure databases

SMRiP69931
ModBaseiSearch...

Protein-protein interaction databases

BioGridi4261436, 151 interactors
851316, 5 interactors
ComplexPortaliCPX-1945 DnaA-Hda complex
CPX-1954 Hda-beta clamp complex
DIPiDIP-48006N
IntActiP69931, 14 interactors
STRINGi511145.b2496

Proteomic databases

PaxDbiP69931
PRIDEiP69931

Genome annotation databases

EnsemblBacteriaiAAC75549; AAC75549; b2496
BAA16384; BAA16384; BAA16384
GeneIDi946977
KEGGiecj:JW5397
eco:b2496
PATRICifig|511145.12.peg.2592

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB3953

Phylogenomic databases

eggNOGiENOG4108KZ1 Bacteria
COG0593 LUCA
HOGENOMiHOG000256538
InParanoidiP69931
KOiK10763
PhylomeDBiP69931

Enzyme and pathway databases

BioCyciEcoCyc:G7313-MONOMER
ECOL316407:JW5397-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P69931

Family and domain databases

HAMAPiMF_01158 Hda, 1 hit
InterProiView protein in InterPro
IPR020591 Chromosome_initiator_DnaA-like
IPR013317 DnaA
IPR017788 Hda
IPR022864 Hda_Enterobact
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00308 Bac_DnaA, 1 hit
PRINTSiPR00051 DNAA
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR03420 DnaA_homol_Hda, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiHDA_ECOLI
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P69931
Secondary accession number(s): P76570, P76979
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: November 24, 2009
Last modified: November 13, 2019
This is version 118 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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