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UniProtKB - P69922 (FUCI_ECOLI)
Protein
L-fucose isomerase
Gene
fucI
Organism
Escherichia coli (strain K12)
Status
Functioni
Converts the aldose L-fucose into the corresponding ketose L-fuculose. Is also able to convert D-arabinose into D-ribulose, but this isomerase has a higher affinity for fucose and fuculose than for arabinose and ribulose, respectively.
Catalytic activityi
Cofactori
Kineticsi
- KM=17 mM for L-fucose
- KM=35 mM for D-arabinose
- KM=1.4 mM for L-fuculose
- KM=11 mM for D-ribulose
: L-fucose degradation Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-lactaldehyde and glycerone phosphate from L-fucose. This subpathway is part of the pathway L-fucose degradation, which is itself part of Carbohydrate degradation.View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-lactaldehyde and glycerone phosphate from L-fucose, the pathway L-fucose degradation and in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 337 | Proton acceptor1 Publication | 1 | |
Metal bindingi | 337 | Manganese | 1 | |
Active sitei | 361 | Proton acceptor1 Publication | 1 | |
Metal bindingi | 361 | Manganese | 1 | |
Metal bindingi | 528 | Manganese | 1 |
GO - Molecular functioni
- arabinose isomerase activity Source: EcoCyc
- identical protein binding Source: IntAct
- L-fucose isomerase activity Source: EcoCyc
- manganese ion binding Source: UniProtKB-UniRule
GO - Biological processi
- D-arabinose catabolic process Source: EcoCyc
- L-fucose catabolic process Source: EcoCyc
Keywordsi
Molecular function | Isomerase |
Biological process | Carbohydrate metabolism, Fucose metabolism |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:FUCISOM-MONOMER |
BRENDAi | 5.3.1.25, 2026 |
UniPathwayi | UPA00563;UER00624 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:fucI Ordered Locus Names:b2802, JW2773 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000204145 | 1 – 591 | L-fucose isomeraseAdd BLAST | 591 |
Proteomic databases
jPOSTi | P69922 |
PaxDbi | P69922 |
PRIDEi | P69922 |
Expressioni
Inductioni
By L-fucose.
Interactioni
Subunit structurei
Homohexamer.
Binary interactionsi
P69922
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-908978,EBI-908978 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4259466, 8 interactors 850555, 1 interactor |
IntActi | P69922, 2 interactors |
STRINGi | 511145.b2802 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P69922 |
SMRi | P69922 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P69922 |
Family & Domainsi
Sequence similaritiesi
Belongs to the L-fucose isomerase family.Curated
Phylogenomic databases
eggNOGi | COG2407, Bacteria |
HOGENOMi | CLU_033326_1_0_6 |
InParanoidi | P69922 |
OMAi | QDYRACA |
PhylomeDBi | P69922 |
Family and domain databases
Gene3Di | 3.20.14.10, 1 hit 3.40.275.10, 1 hit 3.40.50.1070, 1 hit |
HAMAPi | MF_01254, Fucose_iso, 1 hit |
InterProi | View protein in InterPro IPR004216, Fuc/Ara_isomerase_C IPR038393, Fuc_iso_dom3_sf IPR015888, Fuc_isomerase_C IPR038391, Fucose_iso_dom1_sf IPR012888, Fucose_iso_N1 IPR005763, Fucose_isomerase IPR038392, Fucose_isomerase_dom2_sf IPR009015, Fucose_isomerase_N/cen_sf IPR012889, Fucose_isomerase_N2 |
Pfami | View protein in Pfam PF02952, Fucose_iso_C, 1 hit PF07881, Fucose_iso_N1, 1 hit PF07882, Fucose_iso_N2, 1 hit |
SUPFAMi | SSF50443, SSF50443, 1 hit SSF53743, SSF53743, 1 hit |
TIGRFAMsi | TIGR01089, fucI, 1 hit |
i Sequence
Sequence statusi: Complete.
P69922-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKISLPKIG IRPVIDGRRM GVRESLEEQT MNMAKATAAL LTEKLRHACG
60 70 80 90 100
AAVECVISDT CIAGMAEAAA CEEKFSSQNV GLTITVTPCW CYGSETIDMD
110 120 130 140 150
PTRPKAIWGF NGTERPGAVY LAAALAAHSQ KGIPAFSIYG HDVQDADDTS
160 170 180 190 200
IPADVEEKLL RFARAGLAVA SMKGKSYLSL GGVSMGIAGS IVDHNFFESW
210 220 230 240 250
LGMKVQAVDM TELRRRIDQK IYDEAELEMA LAWADKNFRY GEDENNKQYQ
260 270 280 290 300
RNAEQSRAVL RESLLMAMCI RDMMQGNSKL ADIGRVEESL GYNAIAAGFQ
310 320 330 340 350
GQRHWTDQYP NGDTAEAILN SSFDWNGVRE PFVVATENDS LNGVAMLMGH
360 370 380 390 400
QLTGTAQVFA DVRTYWSPEA IERVTGHKLD GLAEHGIIHL INSGSAALDG
410 420 430 440 450
SCKQRDSEGN PTMKPHWEIS QQEADACLAA TEWCPAIHEY FRGGGYSSRF
460 470 480 490 500
LTEGGVPFTM TRVNIIKGLG PVLQIAEGWS VELPKDVHDI LNKRTNSTWP
510 520 530 540 550
TTWFAPRLTG KGPFTDVYSV MANWGANHGV LTIGHVGADF ITLASMLRIP
560 570 580 590
VCMHNVEETK VYRPSAWAAH GMDIEGQDYR ACQNYGPLYK R
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 8 | K → P AA sequence (PubMed:8052131).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15025 Genomic DNA Translation: CAA33127.1 U29581 Genomic DNA Translation: AAB40452.1 U00096 Genomic DNA Translation: AAC75844.1 AP009048 Genomic DNA Translation: BAE76874.1 |
PIRi | JS0185, ISECFI |
RefSeqi | NP_417282.1, NC_000913.3 WP_000724153.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75844; AAC75844; b2802 BAE76874; BAE76874; BAE76874 |
GeneIDi | 946195 |
KEGGi | ecj:JW2773 eco:b2802 |
PATRICi | fig|1411691.4.peg.3931 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15025 Genomic DNA Translation: CAA33127.1 U29581 Genomic DNA Translation: AAB40452.1 U00096 Genomic DNA Translation: AAC75844.1 AP009048 Genomic DNA Translation: BAE76874.1 |
PIRi | JS0185, ISECFI |
RefSeqi | NP_417282.1, NC_000913.3 WP_000724153.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1FUI | X-ray | 2.50 | A/B/C/D/E/F | 1-591 | [»] | |
AlphaFoldDBi | P69922 | |||||
SMRi | P69922 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259466, 8 interactors 850555, 1 interactor |
IntActi | P69922, 2 interactors |
STRINGi | 511145.b2802 |
Proteomic databases
jPOSTi | P69922 |
PaxDbi | P69922 |
PRIDEi | P69922 |
Genome annotation databases
EnsemblBacteriai | AAC75844; AAC75844; b2802 BAE76874; BAE76874; BAE76874 |
GeneIDi | 946195 |
KEGGi | ecj:JW2773 eco:b2802 |
PATRICi | fig|1411691.4.peg.3931 |
Organism-specific databases
EchoBASEi | EB0345 |
Phylogenomic databases
eggNOGi | COG2407, Bacteria |
HOGENOMi | CLU_033326_1_0_6 |
InParanoidi | P69922 |
OMAi | QDYRACA |
PhylomeDBi | P69922 |
Enzyme and pathway databases
UniPathwayi | UPA00563;UER00624 |
BioCyci | EcoCyc:FUCISOM-MONOMER |
BRENDAi | 5.3.1.25, 2026 |
Miscellaneous databases
EvolutionaryTracei | P69922 |
PROi | PR:P69922 |
Family and domain databases
Gene3Di | 3.20.14.10, 1 hit 3.40.275.10, 1 hit 3.40.50.1070, 1 hit |
HAMAPi | MF_01254, Fucose_iso, 1 hit |
InterProi | View protein in InterPro IPR004216, Fuc/Ara_isomerase_C IPR038393, Fuc_iso_dom3_sf IPR015888, Fuc_isomerase_C IPR038391, Fucose_iso_dom1_sf IPR012888, Fucose_iso_N1 IPR005763, Fucose_isomerase IPR038392, Fucose_isomerase_dom2_sf IPR009015, Fucose_isomerase_N/cen_sf IPR012889, Fucose_isomerase_N2 |
Pfami | View protein in Pfam PF02952, Fucose_iso_C, 1 hit PF07881, Fucose_iso_N1, 1 hit PF07882, Fucose_iso_N2, 1 hit |
SUPFAMi | SSF50443, SSF50443, 1 hit SSF53743, SSF53743, 1 hit |
TIGRFAMsi | TIGR01089, fucI, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FUCI_ECOLI | |
Accessioni | P69922Primary (citable) accession number: P69922 Secondary accession number(s): P11552, Q2MA32 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 4, 2005 |
Last sequence update: | January 4, 2005 | |
Last modified: | May 25, 2022 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families