UniProtKB - P69910 (DCEB_ECOLI)
Protein
Glutamate decarboxylase beta
Gene
gadB
Organism
Escherichia coli (strain K12)
Status
Functioni
Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed to extremely acidic conditions. The ability to survive transit through the acidic conditions of the stomach is essential for successful colonization of the mammalian host by commensal and pathogenic bacteria.
Miscellaneous
Changing Lys-276 to Ala increases thermal stability and protease resistance. The unfolding temperature is increased by 11 degrees Celsius.
Catalytic activityi
- EC:4.1.1.15
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 62 | Substrate | 1 | |
Binding sitei | 83 | Substrate | 1 | |
Binding sitei | 212 | Pyridoxal phosphate | 1 | |
Binding sitei | 275 | Pyridoxal phosphate | 1 |
GO - Molecular functioni
- glutamate decarboxylase activity Source: EcoCyc
- pyridoxal phosphate binding Source: InterPro
GO - Biological processi
- glutamate catabolic process Source: GO_Central
- intracellular pH elevation Source: EcoCyc
Keywordsi
Molecular function | Decarboxylase, Lyase |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | EcoCyc:GLUTDECARBOXB-MONOMER MetaCyc:GLUTDECARBOXB-MONOMER |
BRENDAi | 4.1.1.15, 2026 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:gadB Ordered Locus Names:b1493, JW1488 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 276 | K → A: Strongly reduces pyridoxal phosphate binding and increases stability of the polypeptide. 1 Publication | 1 | |
Mutagenesisi | 276 | K → H: Abolishes pyridoxal phosphate binding. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000146982 | 1 – 466 | Glutamate decarboxylase betaAdd BLAST | 466 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 276 | N6-(pyridoxal phosphate)lysine | 1 | |
Modified residuei | 446 | N6-acetyllysine1 Publication | 1 | |
Modified residuei | 453 | N6-acetyllysine1 Publication | 1 | |
Modified residuei | 464 | N6-acetyllysine1 Publication | 1 |
Keywords - PTMi
AcetylationProteomic databases
jPOSTi | P69910 |
PaxDbi | P69910 |
PRIDEi | P69910 |
PTM databases
iPTMneti | P69910 |
Expressioni
Inductioni
By acidic conditions. Expression is regulated by a complex system involving RpoS, cAMP, CRP, EvgAS, H-NS, GadE, GadW and GadX. The level of involvement for each regulator varies depending upon the growth phase and the medium.7 Publications
Interactioni
Subunit structurei
Homohexamer composed of three dimers.
1 PublicationBinary interactionsi
P69910
With | #Exp. | IntAct |
---|---|---|
yadG [P36879] | 2 | EBI-549514,EBI-550911 |
Protein-protein interaction databases
BioGRIDi | 4260788, 5 interactors 850419, 2 interactors |
DIPi | DIP-36202N |
IntActi | P69910, 16 interactors |
MINTi | P69910 |
STRINGi | 511145.b1493 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P69910 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P69910 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 126 – 127 | Pyridoxal phosphate binding | 2 |
Sequence similaritiesi
Belongs to the group II decarboxylase family.Curated
Phylogenomic databases
eggNOGi | COG0076, Bacteria |
HOGENOMi | CLU_019582_0_0_6 |
InParanoidi | P69910 |
PhylomeDBi | P69910 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit |
InterProi | View protein in InterPro IPR010107, Glutamate_decarboxylase IPR002129, PyrdxlP-dep_de-COase IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR021115, Pyridoxal-P_BS |
PANTHERi | PTHR43321, PTHR43321, 1 hit |
Pfami | View protein in Pfam PF00282, Pyridoxal_deC, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR01788, Glu-decarb-GAD, 1 hit |
PROSITEi | View protein in PROSITE PS00392, DDC_GAD_HDC_YDC, 1 hit |
i Sequence
Sequence statusi: Complete.
P69910-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDKKQVTDLR SELLDSRFGA KSISTIAESK RFPLHEMRDD VAFQIINDEL
60 70 80 90 100
YLDGNARQNL ATFCQTWDDE NVHKLMDLSI NKNWIDKEEY PQSAAIDLRC
110 120 130 140 150
VNMVADLWHA PAPKNGQAVG TNTIGSSEAC MLGGMAMKWR WRKRMEAAGK
160 170 180 190 200
PTDKPNLVCG PVQICWHKFA RYWDVELREI PMRPGQLFMD PKRMIEACDE
210 220 230 240 250
NTIGVVPTFG VTYTGNYEFP QPLHDALDKF QADTGIDIDM HIDAASGGFL
260 270 280 290 300
APFVAPDIVW DFRLPRVKSI SASGHKFGLA PLGCGWVIWR DEEALPQELV
310 320 330 340 350
FNVDYLGGQI GTFAINFSRP AGQVIAQYYE FLRLGREGYT KVQNASYQVA
360 370 380 390 400
AYLADEIAKL GPYEFICTGR PDEGIPAVCF KLKDGEDPGY TLYDLSERLR
410 420 430 440 450
LRGWQVPAFT LGGEATDIVV MRIMCRRGFE MDFAELLLED YKASLKYLSD
460
HPKLQGIAQQ NSFKHT
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M84025 Genomic DNA Translation: AAA23834.1 U00096 Genomic DNA Translation: AAC74566.1 AP009048 Genomic DNA Translation: BAA15163.1 X71917 Genomic DNA Translation: CAA50736.1 Sequence problems. |
PIRi | B43332 |
RefSeqi | NP_416010.1, NC_000913.3 WP_000358930.1, NZ_SSUV01000024.1 |
Genome annotation databases
EnsemblBacteriai | AAC74566; AAC74566; b1493 BAA15163; BAA15163; BAA15163 |
GeneIDi | 58391401 946058 |
KEGGi | ecj:JW1488 eco:b1493 |
PATRICi | fig|1411691.4.peg.774 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M84025 Genomic DNA Translation: AAA23834.1 U00096 Genomic DNA Translation: AAC74566.1 AP009048 Genomic DNA Translation: BAA15163.1 X71917 Genomic DNA Translation: CAA50736.1 Sequence problems. |
PIRi | B43332 |
RefSeqi | NP_416010.1, NC_000913.3 WP_000358930.1, NZ_SSUV01000024.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1PMM | X-ray | 2.00 | A/B/C/D/E/F | 1-466 | [»] | |
1PMO | X-ray | 2.30 | A/B/C/D/E/F | 1-466 | [»] | |
2DGK | X-ray | 1.90 | A/B/C/D/E/F | 15-466 | [»] | |
2DGL | X-ray | 3.15 | A/B/C/D/E/F | 1-466 | [»] | |
2DGM | X-ray | 1.95 | A/B/C/D/E/F | 1-466 | [»] | |
3FZ6 | X-ray | 2.82 | A/B/C/D/E/F | 1-466 | [»] | |
3FZ7 | X-ray | 2.50 | A/B/C/D/E/F | 1-466 | [»] | |
3FZ8 | X-ray | 3.00 | A/B/C/D/E/F | 1-466 | [»] | |
SMRi | P69910 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260788, 5 interactors 850419, 2 interactors |
DIPi | DIP-36202N |
IntActi | P69910, 16 interactors |
MINTi | P69910 |
STRINGi | 511145.b1493 |
PTM databases
iPTMneti | P69910 |
Proteomic databases
jPOSTi | P69910 |
PaxDbi | P69910 |
PRIDEi | P69910 |
Genome annotation databases
EnsemblBacteriai | AAC74566; AAC74566; b1493 BAA15163; BAA15163; BAA15163 |
GeneIDi | 58391401 946058 |
KEGGi | ecj:JW1488 eco:b1493 |
PATRICi | fig|1411691.4.peg.774 |
Organism-specific databases
EchoBASEi | EB1453 |
Phylogenomic databases
eggNOGi | COG0076, Bacteria |
HOGENOMi | CLU_019582_0_0_6 |
InParanoidi | P69910 |
PhylomeDBi | P69910 |
Enzyme and pathway databases
BioCyci | EcoCyc:GLUTDECARBOXB-MONOMER MetaCyc:GLUTDECARBOXB-MONOMER |
BRENDAi | 4.1.1.15, 2026 |
Miscellaneous databases
EvolutionaryTracei | P69910 |
PROi | PR:P69910 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit |
InterProi | View protein in InterPro IPR010107, Glutamate_decarboxylase IPR002129, PyrdxlP-dep_de-COase IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR021115, Pyridoxal-P_BS |
PANTHERi | PTHR43321, PTHR43321, 1 hit |
Pfami | View protein in Pfam PF00282, Pyridoxal_deC, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR01788, Glu-decarb-GAD, 1 hit |
PROSITEi | View protein in PROSITE PS00392, DDC_GAD_HDC_YDC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DCEB_ECOLI | |
Accessioni | P69910Primary (citable) accession number: P69910 Secondary accession number(s): P28302, P76873 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 4, 2005 |
Last sequence update: | January 4, 2005 | |
Last modified: | February 10, 2021 | |
This is version 134 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families