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Entry version 120 (05 Jun 2019)
Sequence version 1 (10 May 2005)
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Protein

Multiphosphoryl transfer protein

Gene

fruB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II FruAB PTS system is involved in fructose transport.1 Publication1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei62Tele-phosphohistidine intermediate; for EIIA activityPROSITE-ProRule annotation1
Active sitei299Pros-phosphohistidine intermediate; for HPr activityPROSITE-ProRule annotation1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionKinase, Transferase
Biological processPhosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:FRUB-MONOMER
ECOL316407:JW2156-MONOMER
MetaCyc:FRUB-MONOMER

Protein family/group databases

Transport Classification Database

More...
TCDBi
4.A.2.1.1 the pts fructose-mannitol (fru) family

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Multiphosphoryl transfer proteinBy similarity
Short name:
MTPBy similarity
Alternative name(s):
Diphosphoryl transfer protein1 Publication
Short name:
DTP1 Publication
Phosphotransferase FPr proteinBy similarity
Pseudo-HPrBy similarity
Including the following 2 domains:
Phosphocarrier protein HPrBy similarity
Short name:
Protein HBy similarity
PTS system fructose-specific EIIA component1 Publication
Alternative name(s):
EIIA-FruBy similarity
EIII-FruBy similarity
Fructose-specific phosphotransferase enzyme IIA component1 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:fruB1 Publication
Synonyms:fpr, fruF
Ordered Locus Names:b2169, JW2156
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

Escherichia coli strain K12 genome database

More...
EcoGenei
EG10005 fruB

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001865161 – 376Multiphosphoryl transfer proteinAdd BLAST376

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei62Phosphohistidine; by HPrCurated1
Modified residuei299Phosphohistidine; by EICurated1

Keywords - PTMi

Phosphoprotein

Proteomic databases

Encyclopedia of Proteome Dynamics

More...
EPDi
P69811

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
P69811

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P69811

PRoteomics IDEntifications database

More...
PRIDEi
P69811

2D gel databases

Two-dimensional polyacrylamide gel electrophoresis database from the Geneva University Hospital

More...
SWISS-2DPAGEi
P69811

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Induced by fructose and repressed by FruR.By similarity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
4260464, 19 interactors

Database of interacting proteins

More...
DIPi
DIP-48096N

Protein interaction database and analysis system

More...
IntActi
P69811, 7 interactors

STRING: functional protein association networks

More...
STRINGi
511145.b2169

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P69811

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini2 – 142PTS EIIA type-2PROSITE-ProRule annotationAdd BLAST141
Domaini285 – 375HPrPROSITE-ProRule annotationAdd BLAST91

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni156 – 284M domainBy similarityAdd BLAST129

<p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The PTS EIIA type-2 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the PTS EIIB type-2 domain.PROSITE-ProRule annotation
In contrast to classical PTS systems, the fructose-specific PTS has no requirement for HPr; FruB combines a IIA domain with a HPr domain.1 Publication

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4105DTZ Bacteria
COG1925 LUCA
COG4668 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000278131

KEGG Orthology (KO)

More...
KOi
K02768
K11183

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00367 PTS-HPr_like, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.30.1340.10, 1 hit
3.40.930.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR000032 HPr-like
IPR035895 HPr-like_sf
IPR016152 PTrfase/Anion_transptr
IPR002178 PTS_EIIA_type-2_dom
IPR001020 PTS_HPr_His_P_site
IPR002114 PTS_HPr_Ser_P_site

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00381 PTS-HPr, 1 hit
PF00359 PTS_EIIA_2, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR00107 PHOSPHOCPHPR

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF55594 SSF55594, 1 hit
SSF55804 SSF55804, 2 hits

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR01003 PTS_HPr_family, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51094 PTS_EIIA_TYPE_2, 1 hit
PS00372 PTS_EIIA_TYPE_2_HIS, 1 hit
PS51350 PTS_HPR_DOM, 1 hit
PS00369 PTS_HPR_HIS, 1 hit
PS00589 PTS_HPR_SER, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P69811-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFQLSVQDIH PGEKAGDKEE AIRQVAAALV QAGNVAEGYV NGMLAREQQT
60 70 80 90 100
STFLGNGIAI PHGTTDTRDQ VLKTGVQVFQ FPEGVTWGDG QVAYVAIGIA
110 120 130 140 150
ASSDEHLGLL RQLTHVLSDD SVAEQLKSAT TAEELRALLM GEKQSEQLKL
160 170 180 190 200
DNEMLTLDIV ASDLLTLQAL NAARLKEAGA VDATFVTKAI NEQPLNLGQG
210 220 230 240 250
IWLSDSAEGN LRSAIAVSRA ANAFDVDGET AAMLVSVAMN DDQPIAVLKR
260 270 280 290 300
LADLLLDNKA DRLLKADAAT LLALLTSDDA PTDDVLSAEF VVRNEHGLHA
310 320 330 340 350
RPGTMLVNTI KQFNSDITVT NLDGTGKPAN GRSLMKVVAL GVKKGHRLRF
360 370
TAQGADAEQA LKAIGDAIAA GLGEGA
Length:376
Mass (Da):39,648
Last modified:May 10, 2005 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iD4D953E69E2090FF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti59A → T in BAA15978 (PubMed:9097040).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
S72443 Genomic DNA Translation: AAB31084.1
U00007 Genomic DNA Translation: AAA60534.1
U00096 Genomic DNA Translation: AAC75230.1
AP009048 Genomic DNA Translation: BAA15978.2
X53948 Genomic DNA Translation: CAA37895.1

Protein sequence database of the Protein Information Resource

More...
PIRi
I53564

NCBI Reference Sequences

More...
RefSeqi
NP_416674.1, NC_000913.3
WP_000487246.1, NZ_LN832404.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC75230; AAC75230; b2169
BAA15978; BAA15978; BAA15978

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
946677

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW2156
eco:b2169

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.70

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S72443 Genomic DNA Translation: AAB31084.1
U00007 Genomic DNA Translation: AAA60534.1
U00096 Genomic DNA Translation: AAC75230.1
AP009048 Genomic DNA Translation: BAA15978.2
X53948 Genomic DNA Translation: CAA37895.1
PIRiI53564
RefSeqiNP_416674.1, NC_000913.3
WP_000487246.1, NZ_LN832404.1

3D structure databases

SMRiP69811
ModBaseiSearch...

Protein-protein interaction databases

BioGridi4260464, 19 interactors
DIPiDIP-48096N
IntActiP69811, 7 interactors
STRINGi511145.b2169

Protein family/group databases

TCDBi4.A.2.1.1 the pts fructose-mannitol (fru) family

2D gel databases

SWISS-2DPAGEiP69811

Proteomic databases

EPDiP69811
jPOSTiP69811
PaxDbiP69811
PRIDEiP69811

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75230; AAC75230; b2169
BAA15978; BAA15978; BAA15978
GeneIDi946677
KEGGiecj:JW2156
eco:b2169
PATRICifig|1411691.4.peg.70

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB0005
EcoGeneiEG10005 fruB

Phylogenomic databases

eggNOGiENOG4105DTZ Bacteria
COG1925 LUCA
COG4668 LUCA
HOGENOMiHOG000278131
KOiK02768
K11183

Enzyme and pathway databases

BioCyciEcoCyc:FRUB-MONOMER
ECOL316407:JW2156-MONOMER
MetaCyc:FRUB-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P69811

Family and domain databases

CDDicd00367 PTS-HPr_like, 1 hit
Gene3Di3.30.1340.10, 1 hit
3.40.930.10, 1 hit
InterProiView protein in InterPro
IPR000032 HPr-like
IPR035895 HPr-like_sf
IPR016152 PTrfase/Anion_transptr
IPR002178 PTS_EIIA_type-2_dom
IPR001020 PTS_HPr_His_P_site
IPR002114 PTS_HPr_Ser_P_site
PfamiView protein in Pfam
PF00381 PTS-HPr, 1 hit
PF00359 PTS_EIIA_2, 1 hit
PRINTSiPR00107 PHOSPHOCPHPR
SUPFAMiSSF55594 SSF55594, 1 hit
SSF55804 SSF55804, 2 hits
TIGRFAMsiTIGR01003 PTS_HPr_family, 1 hit
PROSITEiView protein in PROSITE
PS51094 PTS_EIIA_TYPE_2, 1 hit
PS00372 PTS_EIIA_TYPE_2_HIS, 1 hit
PS51350 PTS_HPR_DOM, 1 hit
PS00369 PTS_HPR_HIS, 1 hit
PS00589 PTS_HPR_SER, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPTFAH_ECOLI
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P69811
Secondary accession number(s): P24217, P94759
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: June 5, 2019
This is version 120 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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