UniProtKB - P69805 (PTND_ECOLI)
Protein
PTS system mannose-specific EIID component
Gene
manZ
Organism
Escherichia coli (strain K12)
Status
Functioni
The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II ManXYZ PTS system is involved in mannose transport.2 Publications
Also functions as a receptor for bacterial chemotaxis and is required for infection of the cell by bacteriophage lambda where it most likely functions as a pore for penetration of lambda DNA.2 Publications
GO - Molecular functioni
- protein-N(PI)-phosphohistidine-mannose phosphotransferase system transporter activity Source: EcoCyc
GO - Biological processi
- glucose import across plasma membrane Source: EcoCyc
- mannose transmembrane transport Source: EcoCyc
- phosphoenolpyruvate-dependent sugar phosphotransferase system Source: EcoCyc
Keywordsi
Biological process | Phosphotransferase system, Sugar transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:MANZ-MONOMER MetaCyc:MANZ-MONOMER |
Protein family/group databases
TCDBi | 4.A.6.1.1, the pts mannose-fructose-sorbose (man) family |
Names & Taxonomyi
Protein namesi | Recommended name: PTS system mannose-specific EIID component1 PublicationAlternative name(s): EII-M-Man1 Publication EIID-Man1 Publication Mannose permease IID component1 Publication |
Gene namesi | Name:manZ Synonyms:gptB, ptsM Ordered Locus Names:b1819, JW1808 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 3 Publications; Multi-pass membrane protein 1 Publication
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Other locations
- membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 14 | Cytoplasmic2 Publications1 PublicationAdd BLAST | 14 | |
Intramembranei | 15 – 52 | 1 PublicationAdd BLAST | 38 | |
Topological domaini | 53 – 59 | Cytoplasmic1 Publication | 7 | |
Intramembranei | 60 – 92 | 1 PublicationAdd BLAST | 33 | |
Topological domaini | 93 – 100 | Cytoplasmic1 Publication | 8 | |
Transmembranei | 101 – 140 | 1 PublicationAdd BLAST | 40 | |
Topological domaini | 141 – 144 | Periplasmic1 Publication | 4 | |
Transmembranei | 145 – 173 | 1 PublicationAdd BLAST | 29 | |
Topological domaini | 174 – 183 | Cytoplasmic1 Publication | 10 | |
Transmembranei | 184 – 209 | 1 PublicationAdd BLAST | 26 | |
Topological domaini | 210 – 241 | Periplasmic1 PublicationAdd BLAST | 32 | |
Transmembranei | 242 – 255 | 1 PublicationAdd BLAST | 14 | |
Topological domaini | 256 – 261 | Cytoplasmic1 Publication | 6 | |
Transmembranei | 262 – 280 | 1 PublicationAdd BLAST | 19 | |
Topological domaini | 281 – 283 | Periplasmic1 Publication3 Publications | 3 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000186650 | 1 – 283 | PTS system mannose-specific EIID componentAdd BLAST | 283 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-formylmethionine1 Publication | 1 |
Keywords - PTMi
FormylationProteomic databases
jPOSTi | P69805 |
PaxDbi | P69805 |
PRIDEi | P69805 |
Expressioni
Inductioni
Transcriptionally regulated by the Mlc transcriptional repressor and by the cAMP-CRP complex. Also weakly repressed by NagC.1 Publication
Interactioni
Subunit structurei
Homotrimer of protomers that are composed of two subunits, IIC and IID.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4259146, 324 interactors |
ComplexPortali | CPX-5968, D-mannose-specific enzyme II complex |
IntActi | P69805, 1 interactor |
STRINGi | 511145.b1819 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 11 – 281 | PTS EIIDPROSITE-ProRule annotationAdd BLAST | 271 |
Domaini
The EIID domain, with its homologous EIIC domain, forms the PTS system translocation channel and contains part of its specific substrate-binding site.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG3716, Bacteria |
HOGENOMi | CLU_060742_2_0_6 |
InParanoidi | P69805 |
Family and domain databases
InterProi | View protein in InterPro IPR004704, PTS_IID_man |
Pfami | View protein in Pfam PF03613, EIID-AGA, 1 hit |
TIGRFAMsi | TIGR00828, EIID-AGA, 1 hit |
PROSITEi | View protein in PROSITE PS51108, PTS_EIID, 1 hit |
i Sequence
Sequence statusi: Complete.
P69805-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVDTTQTTTE KKLTQSDIRG VFLRSNLFQG SWNFERMQAL GFCFSMVPAI
60 70 80 90 100
RRLYPENNEA RKQAIRRHLE FFNTQPFVAA PILGVTLALE EQRANGAEID
110 120 130 140 150
DGAINGIKVG LMGPLAGVGD PIFWGTVRPV FAALGAGIAM SGSLLGPLLF
160 170 180 190 200
FILFNLVRLA TRYYGVAYGY SKGIDIVKDM GGGFLQKLTE GASILGLFVM
210 220 230 240 250
GALVNKWTHV NIPLVVSRIT DQTGKEHVTT VQTILDQLMP GLVPLLLTFA
260 270 280
CMWLLRKKVN PLWIIVGFFV IGIAGYACGL LGL
Sequence cautioni
The sequence AAA24445 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence BAA15631 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02699 Genomic DNA Translation: AAA24445.1 Different initiation. U00096 Genomic DNA Translation: AAC74889.2 AP009048 Genomic DNA Translation: BAA15631.1 Different initiation. |
PIRi | A30288, WQECMM |
RefSeqi | NP_416333.4, NC_000913.3 WP_000228655.1, NZ_STEB01000009.1 |
Genome annotation databases
EnsemblBacteriai | AAC74889; AAC74889; b1819 BAA15631; BAA15631; BAA15631 |
GeneIDi | 57731216 946342 |
KEGGi | ecj:JW1808 eco:b1819 |
PATRICi | fig|511145.12.peg.1896 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02699 Genomic DNA Translation: AAA24445.1 Different initiation. U00096 Genomic DNA Translation: AAC74889.2 AP009048 Genomic DNA Translation: BAA15631.1 Different initiation. |
PIRi | A30288, WQECMM |
RefSeqi | NP_416333.4, NC_000913.3 WP_000228655.1, NZ_STEB01000009.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6K1H | electron microscopy | 3.52 | C/F/Z | 1-283 | [»] | |
SMRi | P69805 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259146, 324 interactors |
ComplexPortali | CPX-5968, D-mannose-specific enzyme II complex |
IntActi | P69805, 1 interactor |
STRINGi | 511145.b1819 |
Protein family/group databases
TCDBi | 4.A.6.1.1, the pts mannose-fructose-sorbose (man) family |
Proteomic databases
jPOSTi | P69805 |
PaxDbi | P69805 |
PRIDEi | P69805 |
Genome annotation databases
EnsemblBacteriai | AAC74889; AAC74889; b1819 BAA15631; BAA15631; BAA15631 |
GeneIDi | 57731216 946342 |
KEGGi | ecj:JW1808 eco:b1819 |
PATRICi | fig|511145.12.peg.1896 |
Organism-specific databases
EchoBASEi | EB0564 |
Phylogenomic databases
eggNOGi | COG3716, Bacteria |
HOGENOMi | CLU_060742_2_0_6 |
InParanoidi | P69805 |
Enzyme and pathway databases
BioCyci | EcoCyc:MANZ-MONOMER MetaCyc:MANZ-MONOMER |
Miscellaneous databases
PROi | PR:P69805 |
Family and domain databases
InterProi | View protein in InterPro IPR004704, PTS_IID_man |
Pfami | View protein in Pfam PF03613, EIID-AGA, 1 hit |
TIGRFAMsi | TIGR00828, EIID-AGA, 1 hit |
PROSITEi | View protein in PROSITE PS51108, PTS_EIID, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PTND_ECOLI | |
Accessioni | P69805Primary (citable) accession number: P69805 Secondary accession number(s): P08188 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | November 16, 2011 | |
Last modified: | April 7, 2021 | |
This is version 114 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references