UniProtKB - P69797 (PTNAB_ECOLI)
PTS system mannose-specific EIIAB component
manX
Functioni
The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II ManXYZ PTS system is involved in mannose transport.
4 PublicationsAlso functions as a receptor for bacterial chemotaxis and is required for infection of the cell by bacteriophage lambda where it most likely functions as a pore for penetration of lambda DNA.
2 PublicationsCatalytic activityi
- D-mannose(out) + Nπ-phospho-L-histidyl-[protein] = D-mannose 6-phosphate(in) + L-histidyl-[protein]2 PublicationsEC:2.7.1.1912 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 10 | Tele-phosphohistidine intermediate; for EIIA activityPROSITE-ProRule annotation4 Publications | 1 | |
Sitei | 89 | Involved in the phosphoryl transfer between H-10 and H-1751 Publication | 1 | |
Active sitei | 175 | Pros-phosphohistidine intermediate; for EIIB activity2 Publications | 1 |
GO - Molecular functioni
- kinase activity Source: EcoCyc
- protein-N(PI)-phosphohistidine-mannose phosphotransferase system transporter activity Source: EcoCyc
GO - Biological processi
- glucose import across plasma membrane Source: EcoCyc
- mannose transmembrane transport Source: EcoCyc
- phosphoenolpyruvate-dependent sugar phosphotransferase system Source: ComplexPortal
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Phosphotransferase system, Sugar transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:MANX-MONOMER |
Protein family/group databases
TCDBi | 4.A.6.1.1, the pts mannose-fructose-sorbose (man) family |
Names & Taxonomyi
Protein namesi | Recommended name: PTS system mannose-specific EIIAB component1 Publication (EC:2.7.1.1912 Publications)Alternative name(s): EIIAB-Man1 Publication EIII-Man1 Publication Including the following 2 domains: Mannose-specific phosphotransferase enzyme IIA component1 Publication Alternative name(s): PTS system mannose-specific EIIA component1 Publication Mannose-specific phosphotransferase enzyme IIB component1 Publication Alternative name(s): PTS system mannose-specific EIIB component1 Publication |
Gene namesi | Name:manX1 Publication Synonyms:gptB, ptsL1 Publication Ordered Locus Names:b1817, JW1806 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 2 Publications; Peripheral membrane protein 2 Publications
Cytoplasm and Cytosol
- Cytoplasm 3 Publications
Cytosol
- cytosol Source: EcoCyc
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
Other locations
- cytoplasm Source: EcoCyc
- membrane Source: UniProtKB
- transmembrane transporter complex Source: ComplexPortal
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, Cytoplasm, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 10 | H → C: Loss of phosphotransferase activity. Unable to dimerize. 1 Publication | 1 | |
Mutagenesisi | 10 | H → E: Results in the formation of a single complex corresponding to the productive phosphoryl transfer complex. 1 Publication | 1 | |
Mutagenesisi | 12 | W → F: Slight phosphotransferase activity. Unable to dimerize. 1 Publication | 1 | |
Mutagenesisi | 48 | K → C: Retains more than 50% of phosphotransferase activity. 1 Publication | 1 | |
Mutagenesisi | 72 | S → C: Slight phosphotransferase activity. Unable to dimerize. 1 Publication | 1 | |
Mutagenesisi | 86 | H → N: Loss of phosphotransferase activity. 1 Publication | 1 | |
Mutagenesisi | 110 | S → C: Retains more than 50% of phosphotransferase activity. 1 Publication | 1 | |
Mutagenesisi | 175 | H → C: Loss of phosphotransferase activity. 1 Publication | 1 | |
Mutagenesisi | 219 | H → Q: Slight phosphotransferase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication1 Publication | |||
ChainiPRO_0000186653 | 2 – 323 | PTS system mannose-specific EIIAB componentAdd BLAST | 322 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 10 | Phosphohistidine; by HPr2 Publications | 1 | |
Modified residuei | 55 | N6-acetyllysine1 Publication | 1 | |
Modified residuei | 175 | Phosphohistidine; by EIIAPROSITE-ProRule annotation2 Publications | 1 | |
Modified residuei | 234 | N6-acetyllysine1 Publication | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | P69797 |
PaxDbi | P69797 |
PRIDEi | P69797 |
2D gel databases
SWISS-2DPAGEi | P69797 |
PTM databases
iPTMneti | P69797 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Homodimer.
1 Publication4 PublicationsBinary interactionsi
P69797
With | #Exp. | IntAct |
---|---|---|
cytR [P0ACN7] | 2 | EBI-554089,EBI-1125696 |
Protein-protein interaction databases
BioGRIDi | 4259144, 308 interactors 850691, 2 interactors |
ComplexPortali | CPX-5968, D-mannose-specific enzyme II complex |
DIPi | DIP-35846N |
IntActi | P69797, 15 interactors |
MINTi | P69797 |
STRINGi | 511145.b1817 |
Structurei
Secondary structure
3D structure databases
SMRi | P69797 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P69797 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 124 | PTS EIIA type-4PROSITE-ProRule annotation2 PublicationsAdd BLAST | 123 | |
Domaini | 157 – 320 | PTS EIIB type-4PROSITE-ProRule annotation1 PublicationAdd BLAST | 164 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 137 – 155 | Hinge1 PublicationAdd BLAST | 19 |
Domaini
Phylogenomic databases
eggNOGi | COG2893, Bacteria COG3444, Bacteria |
HOGENOMi | CLU_074797_0_0_6 |
InParanoidi | P69797 |
PhylomeDBi | P69797 |
Family and domain databases
CDDi | cd00006, PTS_IIA_man, 1 hit cd00001, PTS_IIB_man, 1 hit |
Gene3Di | 3.40.35.10, 1 hit 3.40.50.510, 1 hit |
InterProi | View protein in InterPro IPR013789, PTS_EIIA_man IPR004701, PTS_EIIA_man-typ IPR036662, PTS_EIIA_man-typ_sf IPR033887, PTS_IIA_man IPR004720, PTS_IIB_sorbose-sp IPR036667, PTS_IIB_sorbose-sp_sf IPR018455, PTS_IIB_sorbose-sp_subgr |
Pfami | View protein in Pfam PF03610, EIIA-man, 1 hit PF03830, PTSIIB_sorb, 1 hit |
SUPFAMi | SSF52728, SSF52728, 1 hit SSF53062, SSF53062, 1 hit |
TIGRFAMsi | TIGR00824, EIIA-man, 1 hit TIGR00854, pts-sorbose, 1 hit |
PROSITEi | View protein in PROSITE PS51096, PTS_EIIA_TYPE_4, 1 hit PS51101, PTS_EIIB_TYPE_4, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MTIAIVIGTH GWAAEQLLKT AEMLLGEQEN VGWIDFVPGE NAETLIEKYN
60 70 80 90 100
AQLAKLDTTK GVLFLVDTWG GSPFNAASRI VVDKEHYEVI AGVNIPMLVE
110 120 130 140 150
TLMARDDDPS FDELVALAVE TGREGVKALK AKPVEKAAPA PAAAAPKAAP
160 170 180 190 200
TPAKPMGPND YMVIGLARID DRLIHGQVAT RWTKETNVSR IIVVSDEVAA
210 220 230 240 250
DTVRKTLLTQ VAPPGVTAHV VDVAKMIRVY NNPKYAGERV MLLFTNPTDV
260 270 280 290 300
ERLVEGGVKI TSVNVGGMAF RQGKTQVNNA VSVDEKDIEA FKKLNARGIE
310 320
LEVRKVSTDP KLKMMDLISK IDK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 2 – 23 | TIAIV…KTAEM → GWGCRAGCLKRQKW in AAA24110 (Ref. 1) CuratedAdd BLAST | 22 | |
Sequence conflicti | 35 | D → N in AAA24110 (Ref. 1) Curated | 1 | |
Sequence conflicti | 101 | T → R in AAA24110 (Ref. 1) Curated | 1 | |
Sequence conflicti | 142 | A → G in AAA24110 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M36404 Genomic DNA Translation: AAA24110.1 J02699 Genomic DNA Translation: AAA24443.1 U00096 Genomic DNA Translation: AAC74887.1 AP009048 Genomic DNA Translation: BAA15624.1 |
PIRi | A30286, WQECM3 |
RefSeqi | NP_416331.1, NC_000913.3 WP_000150543.1, NZ_STEB01000009.1 |
Genome annotation databases
EnsemblBacteriai | AAC74887; AAC74887; b1817 BAA15624; BAA15624; BAA15624 |
GeneIDi | 66674294 946334 |
KEGGi | ecj:JW1806 eco:b1817 |
PATRICi | fig|1411691.4.peg.434 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M36404 Genomic DNA Translation: AAA24110.1 J02699 Genomic DNA Translation: AAA24443.1 U00096 Genomic DNA Translation: AAC74887.1 AP009048 Genomic DNA Translation: BAA15624.1 |
PIRi | A30286, WQECM3 |
RefSeqi | NP_416331.1, NC_000913.3 WP_000150543.1, NZ_STEB01000009.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1PDO | X-ray | 1.70 | A | 2-133 | [»] | |
1VRC | NMR | - | A/B | 1-133 | [»] | |
1VSQ | NMR | - | A/B | 2-134 | [»] | |
C | 159-323 | [»] | ||||
2JZH | NMR | - | A | 154-323 | [»] | |
2JZN | NMR | - | A/B | 2-134 | [»] | |
C | 159-323 | [»] | ||||
2JZO | NMR | - | A/B | 2-134 | [»] | |
D | 159-323 | [»] | ||||
SMRi | P69797 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259144, 308 interactors 850691, 2 interactors |
ComplexPortali | CPX-5968, D-mannose-specific enzyme II complex |
DIPi | DIP-35846N |
IntActi | P69797, 15 interactors |
MINTi | P69797 |
STRINGi | 511145.b1817 |
Protein family/group databases
TCDBi | 4.A.6.1.1, the pts mannose-fructose-sorbose (man) family |
PTM databases
iPTMneti | P69797 |
2D gel databases
SWISS-2DPAGEi | P69797 |
Proteomic databases
jPOSTi | P69797 |
PaxDbi | P69797 |
PRIDEi | P69797 |
Genome annotation databases
EnsemblBacteriai | AAC74887; AAC74887; b1817 BAA15624; BAA15624; BAA15624 |
GeneIDi | 66674294 946334 |
KEGGi | ecj:JW1806 eco:b1817 |
PATRICi | fig|1411691.4.peg.434 |
Organism-specific databases
EchoBASEi | EB0562 |
Phylogenomic databases
eggNOGi | COG2893, Bacteria COG3444, Bacteria |
HOGENOMi | CLU_074797_0_0_6 |
InParanoidi | P69797 |
PhylomeDBi | P69797 |
Enzyme and pathway databases
BioCyci | EcoCyc:MANX-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P69797 |
PROi | PR:P69797 |
Family and domain databases
CDDi | cd00006, PTS_IIA_man, 1 hit cd00001, PTS_IIB_man, 1 hit |
Gene3Di | 3.40.35.10, 1 hit 3.40.50.510, 1 hit |
InterProi | View protein in InterPro IPR013789, PTS_EIIA_man IPR004701, PTS_EIIA_man-typ IPR036662, PTS_EIIA_man-typ_sf IPR033887, PTS_IIA_man IPR004720, PTS_IIB_sorbose-sp IPR036667, PTS_IIB_sorbose-sp_sf IPR018455, PTS_IIB_sorbose-sp_subgr |
Pfami | View protein in Pfam PF03610, EIIA-man, 1 hit PF03830, PTSIIB_sorb, 1 hit |
SUPFAMi | SSF52728, SSF52728, 1 hit SSF53062, SSF53062, 1 hit |
TIGRFAMsi | TIGR00824, EIIA-man, 1 hit TIGR00854, pts-sorbose, 1 hit |
PROSITEi | View protein in PROSITE PS51096, PTS_EIIA_TYPE_4, 1 hit PS51101, PTS_EIIB_TYPE_4, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PTNAB_ECOLI | |
Accessioni | P69797Primary (citable) accession number: P69797 Secondary accession number(s): P08186, Q47350 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 149 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references